BR112016022737A2 - método para cultivar microalgas do gênero aurantiochytrium em meio de cultura sem cloreto e sem sódio para a produção de dha - Google Patents
método para cultivar microalgas do gênero aurantiochytrium em meio de cultura sem cloreto e sem sódio para a produção de dhaInfo
- Publication number
- BR112016022737A2 BR112016022737A2 BR112016022737A BR112016022737A BR112016022737A2 BR 112016022737 A2 BR112016022737 A2 BR 112016022737A2 BR 112016022737 A BR112016022737 A BR 112016022737A BR 112016022737 A BR112016022737 A BR 112016022737A BR 112016022737 A2 BR112016022737 A2 BR 112016022737A2
- Authority
- BR
- Brazil
- Prior art keywords
- free
- chloride
- culture medium
- sodium
- genus
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 3
- 239000001963 growth medium Substances 0.000 title abstract 2
- 241001306132 Aurantiochytrium Species 0.000 title 1
- 241000341438 Aurantiochytrium mangrovei Species 0.000 abstract 2
- 150000002632 lipids Chemical class 0.000 abstract 2
- 239000011734 sodium Substances 0.000 abstract 2
- DVSZKTAMJJTWFG-SKCDLICFSA-N (2e,4e,6e,8e,10e,12e)-docosa-2,4,6,8,10,12-hexaenoic acid Chemical compound CCCCCCCCC\C=C\C=C\C=C\C=C\C=C\C=C\C(O)=O DVSZKTAMJJTWFG-SKCDLICFSA-N 0.000 abstract 1
- GZJLLYHBALOKEX-UHFFFAOYSA-N 6-Ketone, O18-Me-Ussuriedine Natural products CC=CCC=CCC=CCC=CCC=CCC=CCCCC(O)=O GZJLLYHBALOKEX-UHFFFAOYSA-N 0.000 abstract 1
- 239000002028 Biomass Substances 0.000 abstract 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 abstract 1
- -1 chloride (Cl-) ions Chemical class 0.000 abstract 1
- 235000020669 docosahexaenoic acid Nutrition 0.000 abstract 1
- KAUVQQXNCKESLC-UHFFFAOYSA-N docosahexaenoic acid (DHA) Natural products COC(=O)C(C)NOCC1=CC=CC=C1 KAUVQQXNCKESLC-UHFFFAOYSA-N 0.000 abstract 1
- 239000002609 medium Substances 0.000 abstract 1
- 229910052708 sodium Inorganic materials 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6409—Fatty acids
- C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
- C12P7/6434—Docosahexenoic acids [DHA]
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
- A23D9/00—Other edible oils or fats, e.g. shortenings, cooking oils
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/115—Fatty acids or derivatives thereof; Fats or oils
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/10—Production of fats or fatty oils from raw materials by extracting
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Polymers & Plastics (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Food Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Animal Husbandry (AREA)
- General Chemical & Material Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Birds (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Insects & Arthropods (AREA)
- Marine Sciences & Fisheries (AREA)
- Cell Biology (AREA)
- Biomedical Technology (AREA)
- Botany (AREA)
- Nutrition Science (AREA)
- Mycology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Fodder In General (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
a invenção refere-se a um método de cultivo de um protista do gênero aurantiochytrium mangrovei. o gênero é caracterizado geneticamente e em virtude do seu perfil lipídico. o método torna possível obter um alto rendimento de biomassa e enriquecimento dos protistas assim cultivados em um lipídio e, mais particularmente, em ácido docosa-hexaenoico (dha). a invenção se refere ao desenvolvimento de um meio de cultura que permite a produção, com alta densidade celular, de um protista rico em dha do gênero aurantiochytrium mangrovei. o meio é quimicamente definido com baixo teor de íons sódio (na+) e cloreto (cl-).
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR1452960A FR3019559B1 (fr) | 2014-04-03 | 2014-04-03 | Procede de culture des microalgues du genre aurantiochytrium dans un milieu de culture a teneur reduite en sodium et en chlorure pour la production de dha |
| PCT/FR2015/050881 WO2015150716A2 (fr) | 2014-04-03 | 2015-04-03 | Procede de culture des microalgues du genre aurantiochytrium dans un milieu de culture sans chlorure et sans sodium pour la production de dha |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR112016022737A2 true BR112016022737A2 (pt) | 2017-10-31 |
Family
ID=51210550
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112016022737A BR112016022737A2 (pt) | 2014-04-03 | 2015-04-03 | método para cultivar microalgas do gênero aurantiochytrium em meio de cultura sem cloreto e sem sódio para a produção de dha |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US10100340B2 (pt) |
| EP (1) | EP3126508A2 (pt) |
| JP (1) | JP6705754B2 (pt) |
| KR (1) | KR102502571B1 (pt) |
| CN (1) | CN106795538B (pt) |
| AU (2) | AU2015242493A1 (pt) |
| BR (1) | BR112016022737A2 (pt) |
| CA (1) | CA2944546C (pt) |
| FR (1) | FR3019559B1 (pt) |
| MY (1) | MY178364A (pt) |
| UA (1) | UA124963C2 (pt) |
| WO (1) | WO2015150716A2 (pt) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11122817B2 (en) | 2014-07-25 | 2021-09-21 | Smallfood Inc. | Protein rich food ingredient from biomass and methods of production |
| US11213048B2 (en) | 2014-07-25 | 2022-01-04 | Smallfood, Inc. | Protein rich food ingredient from biomass and methods of preparation |
| FR3045069B1 (fr) * | 2015-12-14 | 2019-01-25 | Metabolium | Procede d'enrichissement de protistes en lipides riches en acides gras polyinsatures, plus particulierement de classe omega 3, et sa mise en oeuvre pour la production de ces lipides |
| ES2617081B1 (es) * | 2015-12-15 | 2018-03-23 | Interquim, S.A. | Procedimiento para la producción de dha |
| JP6865456B2 (ja) * | 2016-05-17 | 2021-04-28 | 株式会社ＭｏＢｉｏｌ藻類研究所 | 飼料に好適なアミノ酸組成である海産従属栄養性藻類を飼料のタンパク質成分として利用する方法 |
| CN110168071A (zh) | 2016-12-22 | 2019-08-23 | 玛拉可再生能源公司 | 用于使用真核微生物产生富含dha、棕榈酸和蛋白质的生物质的方法 |
| WO2019151452A1 (ja) * | 2018-01-31 | 2019-08-08 | 日本水産株式会社 | 微生物の乾燥粉末及びその製造方法 |
| FR3085825B1 (fr) | 2018-09-14 | 2021-07-16 | Fermentalg | Huile de microorganismes riche en acide docosahexaenoique |
| FR3085962B1 (fr) | 2018-09-14 | 2021-06-18 | Fermentalg | Procede d'extracton d'une huile riche en pufa |
| GB201905593D0 (en) * | 2019-04-18 | 2019-06-05 | Bavington Charles | Compositions and uses thereof |
| CN114525312A (zh) * | 2020-11-06 | 2022-05-24 | 嘉必优生物技术（武汉）股份有限公司 | 一种使用裂殖壶菌发酵生产dha的方法 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5340742A (en) | 1988-09-07 | 1994-08-23 | Omegatech Inc. | Process for growing thraustochytrium and schizochytrium using non-chloride salts to produce a microfloral biomass having omega-3-highly unsaturated fatty acids |
| US6410281B1 (en) | 1992-07-10 | 2002-06-25 | Omegatech, Inc. | Reducing corrosion in a fermentor by providing sodium with a non-chloride sodium salt |
| MX338455B (es) * | 2003-10-02 | 2016-04-18 | Dsm Ip Assets Bv | Produccion de altos niveles de dha en microalgas usando cantidades modificadas de cloruro y potasio. |
| DE10352838A1 (de) * | 2003-11-10 | 2005-07-07 | Nutrinova Nutrition Specialties & Food Ingredients Gmbh | Verfahren zur Kultivierung von Mikroorganismen der Gattung Thraustochytriales unter Verwendung eines optimierten Niedrigsalzmediums |
| US7989195B2 (en) * | 2008-02-20 | 2011-08-02 | Washington State University Research Foundation | Heterotrophic algal high cell density production method and system |
| CN101886044A (zh) * | 2010-07-17 | 2010-11-17 | 厦门大学 | 二十二碳六烯酸的制备方法 |
| CA2864277A1 (en) * | 2012-02-13 | 2013-08-22 | Heliae Development Llc | Microalgae enriched with trace minerals |
| FR2988100B1 (fr) * | 2012-03-16 | 2016-02-05 | Fermentalg | Production d'acide docosahexaenoique et d'astaxanthine en mode mixotrophe par schizochytrium |
| JPWO2013176261A1 (ja) * | 2012-05-25 | 2016-01-14 | 味の素株式会社 | 微細藻類を用いた栄養添加剤の製造法 |
| CN103627640B (zh) * | 2013-08-10 | 2018-03-02 | 青岛海智源生命科技有限公司 | 一种培养裂殖壶菌的低氯培养基及用该培养基产生dha的方法 |
-
2014
- 2014-04-03 FR FR1452960A patent/FR3019559B1/fr active Active
-
2015
- 2015-04-03 BR BR112016022737A patent/BR112016022737A2/pt not_active Application Discontinuation
- 2015-04-03 WO PCT/FR2015/050881 patent/WO2015150716A2/fr active Application Filing
- 2015-04-03 EP EP15719804.5A patent/EP3126508A2/fr active Pending
- 2015-04-03 US US15/301,091 patent/US10100340B2/en active Active
- 2015-04-03 CA CA2944546A patent/CA2944546C/fr active Active
- 2015-04-03 UA UAA201609969A patent/UA124963C2/uk unknown
- 2015-04-03 MY MYPI2016703593A patent/MY178364A/en unknown
- 2015-04-03 AU AU2015242493A patent/AU2015242493A1/en not_active Abandoned
- 2015-04-03 CN CN201580017876.XA patent/CN106795538B/zh active Active
- 2015-04-03 KR KR1020167029534A patent/KR102502571B1/ko active IP Right Grant
- 2015-04-03 JP JP2016559541A patent/JP6705754B2/ja active Active
-
2019
- 2019-02-07 AU AU2019200857A patent/AU2019200857B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN106795538A (zh) | 2017-05-31 |
| MY178364A (en) | 2020-10-09 |
| CA2944546C (fr) | 2023-08-15 |
| UA124963C2 (uk) | 2021-12-22 |
| EP3126508A2 (fr) | 2017-02-08 |
| WO2015150716A2 (fr) | 2015-10-08 |
| FR3019559A1 (fr) | 2015-10-09 |
| JP2017511134A (ja) | 2017-04-20 |
| US20170016036A1 (en) | 2017-01-19 |
| AU2019200857A1 (en) | 2019-02-28 |
| WO2015150716A3 (fr) | 2016-02-18 |
| JP6705754B2 (ja) | 2020-06-03 |
| KR20170012205A (ko) | 2017-02-02 |
| AU2019200857B2 (en) | 2020-05-14 |
| CA2944546A1 (fr) | 2015-10-08 |
| KR102502571B1 (ko) | 2023-03-07 |
| FR3019559B1 (fr) | 2018-01-05 |
| AU2015242493A1 (en) | 2016-10-27 |
| US10100340B2 (en) | 2018-10-16 |
| CN106795538B (zh) | 2022-07-08 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B15K | Others concerning applications: alteration of classification |
Ipc: C12P 7/64 (2006.01) |
|
| B06U | Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette] | ||
| B06A | Patent application procedure suspended [chapter 6.1 patent gazette] | ||
| B09B | Patent application refused [chapter 9.2 patent gazette] | ||
| B154 | Notification of filing of divisional application [chapter 15.50 patent gazette] |
Free format text: O PEDIDO FOI DIVIDIDO NO BR122022024842-0 PROTOCOLO 870220113191 EM 05/12/2022 15:38. |
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| B12B | Appeal against refusal [chapter 12.2 patent gazette] |