BR112016014155A2 - ácido nucleico indutor do rna de interferência modificado para não causar ligações inespecíficas e uso do mesmo - Google Patents

ácido nucleico indutor do rna de interferência modificado para não causar ligações inespecíficas e uso do mesmo

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Publication number
BR112016014155A2
BR112016014155A2 BR112016014155A BR112016014155A BR112016014155A2 BR 112016014155 A2 BR112016014155 A2 BR 112016014155A2 BR 112016014155 A BR112016014155 A BR 112016014155A BR 112016014155 A BR112016014155 A BR 112016014155A BR 112016014155 A2 BR112016014155 A2 BR 112016014155A2
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Brazil
Prior art keywords
nucleic acid
interfering rna
inducing nucleic
binding
effects
Prior art date
Application number
BR112016014155A
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English (en)
Inventor
Jang Eun-Sook
Chi Sung-Wook
Original Assignee
Encodegen Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Encodegen Co Ltd filed Critical Encodegen Co Ltd
Priority claimed from PCT/KR2014/011994 external-priority patent/WO2015093769A1/ko
Publication of BR112016014155A2 publication Critical patent/BR112016014155A2/pt

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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
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    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1131—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against viruses
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2310/14—Type of nucleic acid interfering N.A.
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    • C12N2310/34—Spatial arrangement of the modifications
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    • C12N2320/00—Applications; Uses
    • C12N2320/50—Methods for regulating/modulating their activity
    • C12N2320/53—Methods for regulating/modulating their activity reducing unwanted side-effects

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Abstract

a presente invenção refere-se a um ácido nucleico indutor do rna de interferência e o uso do mesmo, e mais particularmente a um ácido nucleico indutor do rna de interferência compreendendo pelo menos uma fita simples de fitas duplas, pelo menos uma fita simples, compreendendo uma modificação substituída por um espaçador, que não é capaz de formar um par de base na região de extremidade 5' ou extremidade 3'. o ácido nucleico indutor do rna de interferência da presente invenção é uma nova forma modificada do nucleotídeo provido para evitar os efeitos ligação-inespecífica, oferecendo um método para reprimir seletivamente a expressão de genes alvo. além disso, de acordo com a presente invenção, o ácido nucleico indutor do rna de interferência provê novas formas modificadas que são seletivas e alvo-específicas como um método para bloquear efeitos ligação-inespecífica enquanto silencia a expressão de genes alvo, enquanto o uso de ácido nucleico de indução de interferência dos rnas convencional causa imprecisão e efeitos adversos por meio das ligações não específicas, assim, o ácido nucleico indutor do rna de interferência foi oferecido para resolver o problema, em que ele será amplamente usado como um método para reprimir a expressão de genes em pesquisa e para terapia de genes sem se preocupar com efeitos ligação-inespecífica.
BR112016014155A 2013-12-17 2014-12-08 ácido nucleico indutor do rna de interferência modificado para não causar ligações inespecíficas e uso do mesmo BR112016014155A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR20130157498 2013-12-17
KR1020140173038A KR101696704B1 (ko) 2013-12-17 2014-12-04 오프-타겟을 막기 위해 변형된 rna 간섭 유도 핵산 및 그 용도
PCT/KR2014/011994 WO2015093769A1 (ko) 2013-12-17 2014-12-08 오프-타겟을 막기 위해 변형된 ｒｎａ 간섭 유도 핵산 및 그 용도

Publications (1)

Publication Number Publication Date
BR112016014155A2 true BR112016014155A2 (pt) 2018-01-09

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ID=53517374

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BR112016014155A BR112016014155A2 (pt) 2013-12-17 2014-12-08 ácido nucleico indutor do rna de interferência modificado para não causar ligações inespecíficas e uso do mesmo

Country Status (10)

Country Link
US (1) US10227592B2 (pt)
EP (1) EP3085784B1 (pt)
JP (2) JP2017502665A (pt)
KR (1) KR101696704B1 (pt)
CN (1) CN105829535B (pt)
AU (1) AU2014367550B2 (pt)
BR (1) BR112016014155A2 (pt)
CA (1) CA2933171C (pt)
MX (1) MX2016007980A (pt)
RU (1) RU2016129053A (pt)

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AU2018216509B2 (en) 2017-01-31 2024-02-29 Curigin Co.,Ltd. Nucleic acid simultaneously inhibiting expression of mTOR gene and STAT3 gene
KR102034764B1 (ko) * 2018-01-17 2019-10-22 (주)큐리진 mTOR 유전자 및 STAT3 유전자의 발현을 동시에 억제하는 핵산
KR102193864B1 (ko) * 2019-05-31 2020-12-22 고려대학교 산학협력단 마이크로rna의 비정규 표적을 억제하는 rna 간섭 유도 핵산 및 그 용도
WO2019231299A1 (ko) * 2018-05-31 2019-12-05 고려대학교 산학협력단 마이크로rna를 억제하는 변형 핵산 및 이의 용도
KR102193874B1 (ko) * 2018-05-31 2020-12-23 고려대학교 산학협력단 마이크로rna를 억제하는 변형 핵산 및 이의 용도
KR102012943B1 (ko) * 2018-05-31 2019-08-21 고려대학교 산학협력단 마이크로rna의 비정규 표적을 억제하는 rna 간섭 유도 핵산 및 그 용도
JP7432929B2 (ja) * 2018-05-31 2024-02-19 コリア ユニバーシティ リサーチ アンド ビジネス ファウンデーション マイクロｒｎａの非正規標的を抑制するｒｎａ干渉誘導核酸およびその用途
WO2021010449A1 (ja) * 2019-07-16 2021-01-21 国立大学法人 東京大学 Ｒｎａ分子、キメラ型ｎａ分子、二本鎖ｒｎａ分子、および二本鎖キメラ型ｎａ分子
US20220401466A1 (en) 2019-08-23 2022-12-22 National University Corporation Tokai National Higher Education And Research System Rna action inhibitor and use thereof
JPWO2022154123A1 (pt) * 2021-01-15 2022-07-21
EP4176061A1 (en) * 2021-01-30 2023-05-10 E-Therapeutics plc Nucleic acids containing abasic nucleotides
TW202321451A (zh) * 2021-08-18 2023-06-01 北京大學 工程化的 adar 招募 rnas 及其使用方法
WO2023055774A1 (en) * 2021-09-30 2023-04-06 Sarepta Therapeutics, Inc. Antisense oligonucleotides having one or more abasic units
CA3232053A1 (en) * 2021-10-08 2023-04-13 E-Therapeutics Plc Nucleic acids containing abasic nucleosides
EP4183825A1 (en) 2021-11-23 2023-05-24 SHPP Global Technologies B.V. Thermoplastic film compositions having improved blue led light stability
CN118159655A (zh) * 2022-01-28 2024-06-07 中国医学科学院基础医学研究所 小rna及其在高血脂治疗中的用途

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Also Published As

Publication number Publication date
RU2016129053A (ru) 2018-01-23
EP3085784A4 (en) 2017-07-19
KR20150070944A (ko) 2015-06-25
JP2019030312A (ja) 2019-02-28
CN105829535A (zh) 2016-08-03
JP6748687B2 (ja) 2020-09-02
US10227592B2 (en) 2019-03-12
AU2014367550B2 (en) 2018-04-19
AU2014367550A1 (en) 2016-07-07
MX2016007980A (es) 2016-12-16
CA2933171C (en) 2022-12-06
CA2933171A1 (en) 2015-06-25
EP3085784A1 (en) 2016-10-26
CN105829535B (zh) 2021-01-15
EP3085784B1 (en) 2020-10-28
US20160304878A1 (en) 2016-10-20
KR101696704B1 (ko) 2017-01-16
JP2017502665A (ja) 2017-01-26

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Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2544 DE 08-10-2019 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.