BR112012032744A2 - moléculas de rna e usos das mesmas. - Google Patents

moléculas de rna e usos das mesmas.

Info

Publication number
BR112012032744A2
BR112012032744A2 BR112012032744A BR112012032744A BR112012032744A2 BR 112012032744 A2 BR112012032744 A2 BR 112012032744A2 BR 112012032744 A BR112012032744 A BR 112012032744A BR 112012032744 A BR112012032744 A BR 112012032744A BR 112012032744 A2 BR112012032744 A2 BR 112012032744A2
Authority
BR
Brazil
Prior art keywords
sequence
encoding
determined
rna molecules
rna
Prior art date
Application number
BR112012032744A
Other languages
English (en)
Inventor
Pal Saetrom
Original Assignee
Mina Therapeutics Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mina Therapeutics Ltd filed Critical Mina Therapeutics Ltd
Publication of BR112012032744A2 publication Critical patent/BR112012032744A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B5/00—ICT specially adapted for modelling or simulations in systems biology, e.g. gene-regulatory networks, protein interaction networks or metabolic networks
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C12N2310/111—Antisense spanning the whole gene, or a large part of it
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Physiology (AREA)
  • Evolutionary Biology (AREA)
  • Medical Informatics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Theoretical Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Oncology (AREA)

Abstract

moléculas de rna e usos das mesmas - a invenção refere-se a um processo de planejamento de uma molécula de rna curta para aumentar a expressão de um gene alvo em uma célula através da regulação para menos de um produto de transcrição de rna não codificador, o dito processo compreendendo as etapas de: a) obtenção da sequência de nucleotídeos do filamento codificador do gene alvo, pelo menos entre 200 nucleotídeos a montante do sítio de in[icio da transcrição do gene e 200 nucleotídeos a jusante do sítio da transcrição do gene; b) determinação da sequência de rna complementar inversa à sequência de nucleotídeos determinada na etapa a); e c) planejamento de uma molécula de rna curta que é o complemento inverso ou possui pelo menos 80% de identidade de sequência com the complemento inverso de uma região da sequência determinada na etapa b); em que o dito processo não inclui uma etapa em que a existência do dito produto da transcrição de rna não codificação é dweterminada; bem como a tais moléculas de rna curtas e usos das mesmas.
BR112012032744A 2010-06-23 2011-06-23 moléculas de rna e usos das mesmas. BR112012032744A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1010557.5A GB201010557D0 (en) 2010-06-23 2010-06-23 RNA molecules and uses thereof
PCT/GB2011/051185 WO2011161460A2 (en) 2010-06-23 2011-06-23 Rna molecules and uses thereof

Publications (1)

Publication Number Publication Date
BR112012032744A2 true BR112012032744A2 (pt) 2015-10-06

Family

ID=42582867

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112012032744A BR112012032744A2 (pt) 2010-06-23 2011-06-23 moléculas de rna e usos das mesmas.

Country Status (7)

Country Link
US (3) US20130164846A1 (pt)
EP (1) EP2585595B8 (pt)
JP (1) JP2013532973A (pt)
CN (1) CN103201384A (pt)
BR (1) BR112012032744A2 (pt)
GB (1) GB201010557D0 (pt)
WO (1) WO2011161460A2 (pt)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2817191A1 (en) 2010-10-08 2012-04-12 Pal Saetrom Short rna molecules
US9920317B2 (en) 2010-11-12 2018-03-20 The General Hospital Corporation Polycomb-associated non-coding RNAs
DK2638163T3 (en) 2010-11-12 2017-07-24 Massachusetts Gen Hospital POLYCOMB-ASSOCIATED NON-CODING RNAs
AU2012308320C1 (en) 2011-09-14 2018-08-23 Translate Bio Ma, Inc. Multimeric oligonucleotide compounds
EA201492122A1 (ru) 2012-05-16 2015-10-30 Рана Терапьютикс, Инк. Композиции и способы для модулирования экспрессии utrn
KR20150030205A (ko) 2012-05-16 2015-03-19 라나 테라퓨틱스, 인크. Smn 유전자 패밀리 발현을 조절하기 위한 조성물 및 방법
US10174323B2 (en) 2012-05-16 2019-01-08 The General Hospital Corporation Compositions and methods for modulating ATP2A2 expression
US10837014B2 (en) 2012-05-16 2020-11-17 Translate Bio Ma, Inc. Compositions and methods for modulating SMN gene family expression
EP2850188A4 (en) 2012-05-16 2016-01-20 Rana Therapeutics Inc COMPOSITIONS AND METHODS FOR MODULATING THE EXPRESSION OF THE MULTIGENIC FAMILY OF HEMOGLOBIN
AU2013262709A1 (en) 2012-05-16 2015-01-22 Rana Therapeutics, Inc. Compositions and methods for modulating MECP2 expression
EP2895200B1 (en) 2012-09-14 2019-11-06 Translate Bio MA, Inc. Multimeric oligonucleotide compounds
CA2921457A1 (en) * 2013-08-16 2015-02-19 Rana Therapeutics, Inc. Heterochromatin forming non-coding rnas
CA2921459A1 (en) 2013-08-16 2015-02-19 Rana Therapeutics, Inc. Oligonucleotides targeting euchromatin regions of genes
WO2015023975A1 (en) * 2013-08-16 2015-02-19 Rana Therapeutics, Inc. Compositions and methods for modulating rna
JP6621409B2 (ja) 2013-11-22 2019-12-18 ミナ セラピューティクス リミテッド Ｃ／ＥＢＰα小分子活性化ＲＮＡ組成物
US20170044540A1 (en) * 2014-04-22 2017-02-16 Mina Therapeutics Limited Sarna compositions and methods of use
WO2016070060A1 (en) 2014-10-30 2016-05-06 The General Hospital Corporation Methods for modulating atrx-dependent gene repression
EP3256591A4 (en) 2015-02-13 2018-08-08 Translate Bio Ma, Inc. Hybrid oligonucleotides and uses thereof
WO2016149455A2 (en) 2015-03-17 2016-09-22 The General Hospital Corporation The rna interactome of polycomb repressive complex 1 (prc1)
WO2016170349A1 (en) * 2015-04-22 2016-10-27 Mina Therapeutics Limited C/ebp alpha sarna compositions and methods of use
WO2016170348A2 (en) * 2015-04-22 2016-10-27 Mina Therapeutics Limited Sarna compositions and methods of use
US20200208152A1 (en) 2017-09-08 2020-07-02 Mina Therapeutics Limited Stabilized sarna compositions and methods of use
WO2019048632A1 (en) 2017-09-08 2019-03-14 Mina Therapeutics Limited STABILIZED COMPOSITIONS OF SMALL ACTIVATORY RNA (PARNA) OF HNF4A AND METHODS OF USE
US20210332366A1 (en) * 2018-04-10 2021-10-28 Ractigen Therapeutics Novel Small Activating RNA
EP4242307A3 (en) 2018-04-12 2023-12-27 MiNA Therapeutics Limited Sirt1-sarna compositions and methods of use
EP3953473A1 (en) 2019-04-12 2022-02-16 MiNA Therapeutics Limited Sirt1-sarna compositions and methods of use
CA3151996A1 (en) 2019-08-19 2021-02-25 Mina Therapeutics Limited Oligonucleotide conjugate compositions and methods of use
AU2022246144A1 (en) 2021-03-26 2023-09-21 Mina Therapeutics Limited Tmem173 sarna compositions and methods of use
WO2023099884A1 (en) * 2021-12-01 2023-06-08 Mina Therapeutics Limited Pax6 sarna compositions and methods of use
WO2023170435A1 (en) 2022-03-07 2023-09-14 Mina Therapeutics Limited Il10 sarna compositions and methods of use
WO2024134199A1 (en) 2022-12-22 2024-06-27 Mina Therapeutics Limited Chemically modified sarna compositions and methods of use

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1386004A4 (en) * 2001-04-05 2005-02-16 Ribozyme Pharm Inc MODULATION OF GENE EXPRESSION ASSOCIATED WITH INFLAMMATORY PROLIFERATION AND GROWTH OF NEURITIES BY METHODS INVOLVING NUCLEIC ACID
EP1976567B1 (en) * 2005-12-28 2020-05-13 The Scripps Research Institute Natural antisense and non-coding rna transcripts as drug targets
JP2010539990A (ja) * 2007-10-04 2010-12-24 ボード オブ リージェンツ ザ ユニバーシティー オブ テキサス システム アンチセンス転写物を標的とするａｇＲＮＡおよびギャップマーを用いた遺伝子発現の調節方法
EP2363467B1 (en) * 2008-10-23 2015-12-16 The University of Tokyo Method for inhibiting function of micro-rna

Also Published As

Publication number Publication date
US20130164846A1 (en) 2013-06-27
CN103201384A (zh) 2013-07-10
WO2011161460A3 (en) 2012-02-16
EP2585595A2 (en) 2013-05-01
US20170107514A1 (en) 2017-04-20
US20140316126A1 (en) 2014-10-23
EP2585595B8 (en) 2017-09-27
GB201010557D0 (en) 2010-08-11
JP2013532973A (ja) 2013-08-22
WO2011161460A2 (en) 2011-12-29
EP2585595B1 (en) 2017-08-16

Similar Documents

Publication Publication Date Title
BR112012032744A2 (pt) moléculas de rna e usos das mesmas.
BR112015022507A2 (pt) ácidos ribonucleicos com nucleotídeos 4'-tio-modificados, composição compreendendo o mesmo e usos relacionados
Ma et al. MicroRNA-466l upregulates IL-10 expression in TLR-triggered macrophages by antagonizing RNA-binding protein tristetraprolin-mediated IL-10 mRNA degradation
BR112016014155A2 (pt) ácido nucleico indutor do rna de interferência modificado para não causar ligações inespecíficas e uso do mesmo
BR112016012264A2 (pt) sonda de ácido nucleico e método de detectar fragmentos genômicos
BR112012013875A2 (pt) preparações de rna compreendendo rna modificado purificado para reprogramar células
Marq et al. Unpaired 5′ ppp-nucleotides, as found in arenavirus double-stranded RNA panhandles, are not recognized by RIG-I
BR112015000710A8 (pt) Oligonucleotídeos para fazer uma alteração na sequência de um presente alvo de molécula de rna em uma célula viva
BRPI0819526B8 (pt) Sequência de ácido nucleico de precursor de mirna artificial, construção recombinante e método para reduzir a expressão de uma sequência alvo
BR112021018159A2 (pt) Método para gerar uma molécula de rna com atividade de silenciamento, célula geneticamente modificada, planta geneticamente modificada, célula vegetal, planta, semente, métodos de produção de uma planta, de tratamento de uma doença e de introdução da atividade de silenciamento
BR112015030491A8 (pt) método de modular a expressão de um ácido nucleico alvo em uma célula, bem como método de alterar um ácido nucleico de dna alvo em uma célula
BR112013031021A2 (pt) composições e métodos para a inibição da expressão de gene do vírus da hepatite b'
Li et al. Identification and characterization of microRNAs in the spleen of common carp immune organ
AR088263A1 (es) Moleculas de acido nucleico que se dirigen a rps6 y confieren resistencia a plagas de coleopteros
WO2012046085A3 (en) Methods of inducing insulin production
BRPI0821034A8 (pt) Vírus influenza b recombinante, seu uso, metodo de preparação, composição de vacina o contendo e seu gene m, bem como ácido nucléico isolado, molécula do vírus influenza m e seu uso e método para aumentar a taxa de crescimento de vírus influenza b deficiente de replicação
Guo et al. Selected isomiR expression profiles via arm switching?
BR112016014162A2 (pt) uso de um conjugado, conjugado e métodos para a fabricação de um conjugado e para transfectar uma célula
BR112014026206A2 (pt) sistemas e métodos celulares para melhorar a síntese de ácidos graxos através da expressão da desidrogenase
BR112013025819A2 (pt) promotor específico para semente em algodão
RU2016129283A (ru) Ras opposite (rop) и родственные молекулы нуклеиновых кислот, которые придают устойчивость жесткокрылым и полужесткокрылым насекомым-вредителям
Zheng et al. Genome-wide discovery and analysis of phased small interfering RNAs in Chinese sacred lotus
Krchňáková et al. On the possibility of an early evolutionary origin for the spliced leader trans-splicing
BR112016013420A2 (pt) células eucarióticas e métodos para expressar recombinantemente um produto de interesse
BR112013014082A2 (pt) "moléculas de rna, micro-rna e de ácido nucleico, composições e construtos compreendendo as mesmas, bem como métodos para melhorar a resistência 5 de uma planta à infecção, controlar a infecção e reduzir o desenvolvimento do cisto de um nematódeo, para produção de uma célula ou de uma planta possuindo resistência a nematódeo e para melhorar a produtividade".

Legal Events

Date Code Title Description
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 4A ANUIDADE.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2344 DE 08-12-2015 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.