BR112015003839A2 - método para produzir lâmina de células do epitélio pigmentar da retina - Google Patents

método para produzir lâmina de células do epitélio pigmentar da retina

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Publication number
BR112015003839A2
BR112015003839A2 BR112015003839A BR112015003839A BR112015003839A2 BR 112015003839 A2 BR112015003839 A2 BR 112015003839A2 BR 112015003839 A BR112015003839 A BR 112015003839A BR 112015003839 A BR112015003839 A BR 112015003839A BR 112015003839 A2 BR112015003839 A2 BR 112015003839A2
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BR
Brazil
Prior art keywords
cells
retinal pigment
angiogenic
layer
sheet
Prior art date
Application number
BR112015003839A
Other languages
English (en)
Other versions
BR112015003839B1 (pt
Inventor
Kamao Hiroyuki
Takahashi Masayo
Original Assignee
Riken
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Riken filed Critical Riken
Publication of BR112015003839A2 publication Critical patent/BR112015003839A2/pt
Publication of BR112015003839B1 publication Critical patent/BR112015003839B1/pt

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/44—Vessels; Vascular smooth muscle cells; Endothelial cells; Endothelial progenitor cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • A61L27/3808—Endothelial cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • A61L27/3834—Cells able to produce different cell types, e.g. hematopoietic stem cells, mesenchymal stem cells, marrow stromal cells, embryonic stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3886—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells comprising two or more cell types
    • A61L27/3891—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells comprising two or more cell types as distinct cell layers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P27/00—Drugs for disorders of the senses
    • A61P27/02—Ophthalmic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0621—Eye cells, e.g. cornea, iris pigmented cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/069—Vascular Endothelial cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2430/00—Materials or treatment for tissue regeneration
    • A61L2430/16—Materials or treatment for tissue regeneration for reconstruction of eye parts, e.g. intraocular lens, cornea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/08—Coculture with; Conditioned medium produced by cells of the nervous system
    • C12N2502/085—Coculture with; Conditioned medium produced by cells of the nervous system eye cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/28—Vascular endothelial cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Medicinal Chemistry (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Epidemiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dermatology (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Botany (AREA)
  • Transplantation (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Urology & Nephrology (AREA)
  • Vascular Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Ophthalmology & Optometry (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)

Abstract

resumo patente de invenção: “método para produzir lâmina de células do epitélio pigmentar da retina”. a invenção refere-se a um método para produzir uma lâmina de células incluindo uma camada de célula do epitélio pigmentar da retina e uma camada de célula angiogênica, o método incluindo uma etapa para laminar uma camada de célula do epitélio pigmentar da retina e uma camada de célula angiogênica construída de células constituintes de vaso sanguíneo ou células-tronco tendo potência angiogênica; e fornece uma lâmina de células obtido por este método. a invenção também fornece uma lâmina de células para transplante incluindo uma camada de célula formada por células do epitélio pigmentar da retina obtidas induzindo-se a diferenciação in vitro de células-tronco ou células progenitoras, uma membrana basal secretada destas células, e uma camada de célula angiogênica construída de células constituintes de vaso sanguíneo ou células-tronco tendo potência angiogênica.
BR112015003839-5A 2012-08-24 2013-08-23 Método para produzir lâmina de células do epitélio pigmentar da retina BR112015003839B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2012-185932 2012-08-24
JP2012185932 2012-08-24
PCT/JP2013/072589 WO2014030749A1 (ja) 2012-08-24 2013-08-23 網膜色素上皮細胞シートの製造方法

Publications (2)

Publication Number Publication Date
BR112015003839A2 true BR112015003839A2 (pt) 2017-07-04
BR112015003839B1 BR112015003839B1 (pt) 2021-12-28

Family

ID=50150052

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112015003839-5A BR112015003839B1 (pt) 2012-08-24 2013-08-23 Método para produzir lâmina de células do epitélio pigmentar da retina

Country Status (12)

Country Link
US (1) US11033586B2 (pt)
EP (1) EP2889374B1 (pt)
JP (1) JP6292557B2 (pt)
KR (1) KR102254082B1 (pt)
CN (2) CN113088481A (pt)
AU (1) AU2013306720B2 (pt)
BR (1) BR112015003839B1 (pt)
CA (1) CA2882802C (pt)
HK (1) HK1210496A1 (pt)
SG (1) SG11201501319PA (pt)
WO (1) WO2014030749A1 (pt)
ZA (1) ZA201501976B (pt)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2721952C (en) * 2008-04-22 2019-11-12 Regenerative Research Foundation Retinal pigment epithelial stem cells
WO2012115244A1 (ja) 2011-02-25 2012-08-30 独立行政法人理化学研究所 網膜色素上皮細胞シートの製造方法
JPWO2015125941A1 (ja) * 2014-02-21 2017-03-30 株式会社ヘリオス 眼疾患治療剤、そのスクリーニング方法または網膜色素上皮細胞移植に伴う拒絶反応の予測方法
CN106282096A (zh) * 2016-10-19 2017-01-04 江苏艾尔康生物医药科技有限公司 一种人类视网膜色素上皮细胞层的分离培养方法
CA3043174A1 (en) 2016-11-09 2018-05-17 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Tissue clamp and implantation method
AU2017359330B2 (en) * 2016-11-09 2022-03-10 The United States Of America, As Represented By The Secretary Department Of Health And Human Services 3D vascularized human ocular tissue for cell therapy and drug discovery
EP3831419A4 (en) * 2018-07-31 2022-04-20 Rokit Healthcare Inc. METHOD FOR MAKING A MULTILAYER CELL SHEET, AND MULTILAYER CELL SHEET MADE BY MEANS OF THE SAME METHOD
CN109464705B (zh) * 2018-11-19 2021-08-17 爱尔眼科医院集团股份有限公司 一种rpe细胞片及其应用和制备方法
CN114450418A (zh) 2019-07-19 2022-05-06 东京毅力科创株式会社 细胞的分化状态的评价方法
WO2023210609A1 (ja) * 2022-04-27 2023-11-02 ファーマバイオ株式会社 細胞シート作製方法
JP2024077544A (ja) * 2022-11-28 2024-06-07 ニッタ株式会社 細胞シート作製装置及び細胞シート作製装置用無菌閉鎖系回路

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH06104061B2 (ja) 1989-02-10 1994-12-21 花王株式会社 細胞培養支持体材料
IL94611A (en) 1989-06-05 1994-12-29 Organogenesis Inc Medium for cell cultures containing insulin or growth factor similar to insulin, transferrin or iron ion, triiodothyronine or thyroxine and method of use
SG49267A1 (en) * 1989-08-14 1998-05-18 Photogenesis Inc Surgical instrument and cell isolation and transplantation
JPH05192138A (ja) 1992-01-22 1993-08-03 Kao Corp 皮膚細胞培養法及び培養皮膚
EP0700429B1 (en) * 1993-04-30 2000-10-11 PHOTOGENESIS Incorporated Retinal pigment epithelium transplantation
KR20110102961A (ko) * 2002-07-25 2011-09-19 더 스크립스 리서치 인스티튜트 조혈 줄기세포 및 이를 사용한 신생혈관 안구 질환의 치료방법
AU2003279132A1 (en) * 2002-10-04 2004-05-04 Tissuetech, Inc. Retinal pigment epithelial cell cultures on amniotic membrane and transplantation
WO2005012506A2 (en) 2003-07-30 2005-02-10 Acucela, Inc. Extended primary retinal cell culture and stress models, and methods of use
CA2550312A1 (en) 2003-12-24 2005-07-21 Pfizer Products Inc. Retinal toxicity screening methods
SG188122A1 (en) * 2004-01-23 2013-03-28 Advanced Cell Tech Inc Improved modalities for the treatment of degenerative diseases of the retina
EP1723974A4 (en) 2004-02-25 2010-09-01 Ihara & Company Ltd COLLAGENGEL AND METHOD FOR ITS MANUFACTURE
JP2005261292A (ja) * 2004-03-18 2005-09-29 Ihara Suisan Kk 細胞シートおよびその製造方法
US20060153815A1 (en) * 2004-12-21 2006-07-13 Agnieszka Seyda Tissue engineering devices for the repair and regeneration of tissue
US7846467B2 (en) * 2005-01-13 2010-12-07 Minas Theodore Coroneo Ocular scaffold for stem cell cultivation and methods of use
WO2008056779A1 (fr) 2006-11-09 2008-05-15 Japan As Represented By The President Of International Medical Center Of Japan Procédé destiné à la culture et au passage d'une cellule souche embryonnaire de primate, et procédé destiné à induire la différenciation de la cellule souche embryonnaire
JP5641669B2 (ja) 2007-03-14 2014-12-17 株式会社セルシード 細胞表層蛋白修復方法
KR100986149B1 (ko) 2007-09-14 2010-10-07 차의과학대학교 산학협력단 저 산소 배양액 조건을 이용한 배아 줄기 세포로부터유래된 배상체로부터 혈관 내피 전구 세포의 분화방법
GB0806746D0 (en) 2008-04-14 2008-05-14 Ucl Business Plc Membrane
CN101629162B (zh) * 2009-08-26 2011-05-25 暨南大学 组织工程细胞片及其制备方法
JP5867926B2 (ja) * 2010-05-10 2016-02-24 公立大学法人名古屋市立大学 細胞シート作製方法
EP3549617B1 (en) * 2010-07-12 2023-09-13 University of Southern California Biocompatible substrate for facilitating interconnections between stem cells and target tissues and methods for implanting same
CN101940591B (zh) * 2010-08-27 2013-09-18 上海士腾生物技术有限公司 促血管再生或新生的制剂及其制备方法
WO2012115244A1 (ja) * 2011-02-25 2012-08-30 独立行政法人理化学研究所 網膜色素上皮細胞シートの製造方法

Also Published As

Publication number Publication date
BR112015003839B1 (pt) 2021-12-28
AU2013306720A1 (en) 2015-03-26
SG11201501319PA (en) 2015-04-29
US20150250828A1 (en) 2015-09-10
JP6292557B2 (ja) 2018-03-14
CA2882802C (en) 2021-07-06
CA2882802A1 (en) 2014-02-27
KR102254082B1 (ko) 2021-05-18
KR20150047538A (ko) 2015-05-04
US11033586B2 (en) 2021-06-15
CN113088481A (zh) 2021-07-09
HK1210496A1 (en) 2016-04-22
CN104755611A (zh) 2015-07-01
JPWO2014030749A1 (ja) 2016-08-08
WO2014030749A1 (ja) 2014-02-27
EP2889374A1 (en) 2015-07-01
EP2889374A4 (en) 2016-03-30
AU2013306720B2 (en) 2018-10-18
EP2889374B1 (en) 2017-12-13
ZA201501976B (en) 2018-11-28

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