BR112014019798B1 - Aparelho e método para automaticamente analisar amostras biológicas - Google Patents
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- BR112014019798B1 BR112014019798B1 BR112014019798-9A BR112014019798A BR112014019798B1 BR 112014019798 B1 BR112014019798 B1 BR 112014019798B1 BR 112014019798 A BR112014019798 A BR 112014019798A BR 112014019798 B1 BR112014019798 B1 BR 112014019798B1
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- pipette
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- nucleic acid
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Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020120013757A KR101762295B1 (ko) | 2012-02-10 | 2012-02-10 | 생체시료의 자동 분석 장치 및 방법 |
| KR10-2012-0013757 | 2012-02-10 | ||
| PCT/KR2013/000985 WO2013119049A1 (ko) | 2012-02-10 | 2013-02-07 | 생물학적 시료의 자동 분석 장치 및 방법 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| BR112014019798A8 BR112014019798A8 (pt) | 2017-07-11 |
| BR112014019798A2 BR112014019798A2 (pt) | 2021-06-01 |
| BR112014019798B1 true BR112014019798B1 (pt) | 2022-03-03 |
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Families Citing this family (104)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10072927B2 (en) | 2016-01-07 | 2018-09-11 | Rarecyte, Inc. | Detecting a substrate |
| CN203866295U (zh) * | 2013-11-01 | 2014-10-08 | 艾康生物技术(杭州)有限公司 | 一种核酸提取仪 |
| WO2015072757A1 (ko) * | 2013-11-15 | 2015-05-21 | 바디텍메드 주식회사 | 일체화된 반응 및 검출 수단을 구비한 시험 장치에 사용되는 스테이션 |
| KR101653302B1 (ko) * | 2014-02-26 | 2016-09-01 | (주)한울티피씨 | 핵산추출장치 |
| WO2015199423A1 (ko) * | 2014-06-24 | 2015-12-30 | (주)바이오니아 | 자성 입자를 이용한 핵산 분리 방법 |
| KR101686049B1 (ko) * | 2014-10-15 | 2016-12-13 | 주식회사 노블바이오 | 유전자 정제 방법 및 그 장치 |
| WO2016117726A1 (en) * | 2015-01-23 | 2016-07-28 | Infopia Co., Ltd. | Cartridge |
| US20160238627A1 (en) | 2015-02-13 | 2016-08-18 | Abbott Laboratories | Decapping and capping apparatus, systems and methods for use in diagnostic analyzers |
| KR101707987B1 (ko) * | 2015-06-16 | 2017-02-21 | 휴스텝스 주식회사 | 자동 정량 분주기 및 상기 분주기를 포함하는 연속식 유전자 추출-증폭 장비와 연속식 유전자 추출-증폭 방법 |
| CN108139420A (zh) * | 2015-08-10 | 2018-06-08 | 考希尔股份有限公司 | 模块化液体处理系统 |
| CN106701527A (zh) * | 2015-08-11 | 2017-05-24 | 广州康昕瑞基因健康科技有限公司 | 一种试剂工作站 |
| CN105085719B (zh) * | 2015-09-09 | 2017-03-22 | 中国科学院华南植物园 | 聚合箱 |
| CN105400680B (zh) * | 2015-12-11 | 2017-12-15 | 舟山医院 | 一种荧光定量pcr仪 |
| EP3187595B1 (en) * | 2015-12-30 | 2019-01-16 | Fundación Gaiker | Method for the detection of legionella spp. strains in environmental samples based on loop-mediated isothermal amplification (lamp), detection reagent and set of primers. |
| WO2017131254A1 (ko) * | 2016-01-28 | 2017-08-03 | (주)바이오필리아 | 유전자 또는 생물학적 물질의 분리 및 검사 키트 |
| CN105624032B (zh) * | 2016-02-25 | 2017-11-28 | 东南大学 | 一种病原体现场快速检测系统 |
| EP3213819B1 (en) * | 2016-03-02 | 2021-09-29 | F. Hoffmann-La Roche AG | Device and method for separation |
| JP6466027B2 (ja) * | 2016-04-20 | 2019-02-13 | シスメックス株式会社 | 核酸分析装置 |
| CN105969899A (zh) * | 2016-07-19 | 2016-09-28 | 湖州高亿诺生物科技有限公司 | 基于多孔板的痕量靶标核酸的富集方法和装置 |
| CN109716048B (zh) * | 2016-07-21 | 2021-05-28 | 西门子医疗保健诊断公司 | 用于存储冷藏的校准和质量控制材料的机构 |
| KR101802807B1 (ko) | 2016-08-10 | 2017-11-29 | (주)플렉스시스템 | 시료 검출장치의 밸브 시스템 |
| KR101866195B1 (ko) * | 2016-08-26 | 2018-06-12 | (주)에스엠션전자 | 휴대용 핵산 증폭장치 및 이를 이용한 핵산 증폭방법 |
| CN106367307A (zh) * | 2016-08-30 | 2017-02-01 | 冯晓均 | 一种自动化核酸定量分析装置及分析方法 |
| SG10201707410VA (en) * | 2016-09-12 | 2018-04-27 | Delta Electronics Intl Singapore Pte Ltd | Nucleic Acid Analysis Apparatus |
| US11426735B2 (en) | 2016-09-12 | 2022-08-30 | Delta Electronics Int'l (Singapore) Pte Ltd | Nucleic acid analysis apparatus |
| US11478791B2 (en) | 2016-09-12 | 2022-10-25 | Delta Electronics Int'l (Singapore) Pte Ltd | Flow control and processing cartridge |
| US11376581B2 (en) | 2016-09-12 | 2022-07-05 | Delta Electronics Int'l (Singapore) Pte Ltd | Flow control and processing cartridge |
| US10850281B2 (en) | 2016-09-12 | 2020-12-01 | Delta Electronics Int'l (Singapore) Pte Ltd | Nucleic acid analysis apparatus |
| WO2018053783A1 (zh) * | 2016-09-23 | 2018-03-29 | 深圳华大智造科技有限公司 | 一种用于制备基因测序样本的系统及其应用 |
| EP3308852B1 (en) * | 2016-10-11 | 2020-12-02 | Ion Beam Applications | Apparatus for the synthesis of radiopharmaceutical products |
| WO2018074614A1 (ko) * | 2016-10-17 | 2018-04-26 | 휴스텝스 주식회사 | 자동 정량 분주기 및 상기 분주기를 포함하는 연속식 유전자 추출-증폭 장비와 연속식 유전자 추출-증폭 방법 |
| JP6694796B2 (ja) * | 2016-10-18 | 2020-05-20 | ヤマハ発動機株式会社 | 細胞移動装置 |
| CN106526202B (zh) * | 2016-11-17 | 2018-07-13 | 百奥森（江苏）食品安全科技有限公司 | 一种封闭式病原体检测卡盒 |
| CN106442454A (zh) * | 2016-11-17 | 2017-02-22 | 中国科学院上海光学精密机械研究所 | 荧光定量基因快速扩增检测装置和扩增检测方法 |
| EP3526578A4 (en) * | 2016-11-18 | 2020-10-28 | SM Research Inc. | BIOLOGICAL SAMPLE PREPARATION SYSTEM AND ASSOCIATED PROCESS |
| EP3568235B1 (en) * | 2017-01-13 | 2024-01-24 | Beckman Coulter, Inc. | Motion systems for loading tips |
| US11173491B2 (en) | 2017-01-25 | 2021-11-16 | U-gene & Cell Co. | Apparatus for high-speed nucleic acid amplification and method for temperature control of nucleic acid amplification reaction |
| KR102081480B1 (ko) * | 2017-01-25 | 2020-04-24 | 주식회사 유진셀 | 고속 핵산증폭 장치 |
| CN106867883B (zh) * | 2017-03-03 | 2019-12-27 | 安徽为臻生物工程技术有限公司 | 一种自动核酸提取仪 |
| CN106676002A (zh) * | 2017-03-08 | 2017-05-17 | 深圳市爱康生物科技有限公司 | 一种应用于自动核酸提取仪的自动磁力架 |
| EP3605107A4 (en) * | 2017-04-28 | 2020-12-30 | Ezdia Tech Inc. | DEVICE AND PROCESS FOR AUTOMATED IMMUNOLOGICAL ASSAY USING A LARGE MAGNETIC PARTICLE COMPLEX |
| KR20190001797A (ko) * | 2017-06-28 | 2019-01-07 | (주)오상헬스케어 | 자동화된 핵산 분리 장치 |
| WO2019004276A1 (en) | 2017-06-29 | 2019-01-03 | Sony Semiconductor Solutions Corporation | BACKLIT IMAGEER LINKED TO A SLICER |
| WO2019017501A1 (ko) * | 2017-07-17 | 2019-01-24 | 주식회사 스몰머신즈 | 표적물질의 정량 분석 방법 및 이를 이용한 표적물질의 정량 분석 디바이스 |
| KR101912942B1 (ko) * | 2017-07-24 | 2018-10-30 | 한국과학기술원 | 전자동 유전자 판별 통합설비 |
| KR102022060B1 (ko) * | 2017-08-29 | 2019-09-19 | 신진메딕스(주) | 카트리지의 자동공급이 가능한 감마카운터 |
| KR102022054B1 (ko) * | 2017-08-29 | 2019-09-18 | 신진메딕스(주) | 시약 공급이 용이한 자동분주기 |
| KR101990599B1 (ko) * | 2017-09-19 | 2019-06-18 | (주)로봇앤드디자인 | Dna 처리 장치 |
| CN107760561B (zh) * | 2017-10-26 | 2019-05-28 | 深圳市汇研科创生物科技有限公司 | 核酸提取装置及核酸提取方法 |
| CN107841450B (zh) * | 2017-12-21 | 2023-09-29 | 山西省农业科学院棉花研究所 | 一种用于核酸提取实验的乙醇清除装置及其使用方法 |
| CN108315231A (zh) * | 2018-01-26 | 2018-07-24 | 楼德耀 | 一种新型生物检测设备 |
| RU2714734C1 (ru) * | 2018-03-19 | 2020-02-19 | Общество с ограниченной активностью "Синтол" (ООО "Синтол") | Устройство для автоматической очистки биологических образцов |
| WO2019195836A1 (en) * | 2018-04-07 | 2019-10-10 | Lumacyte, LLC | Fluidic autosampler and incubator |
| CN108277148B (zh) * | 2018-04-25 | 2024-05-28 | 江苏硕世生物科技股份有限公司 | 核酸提取分液装置 |
| WO2020014296A1 (en) * | 2018-07-12 | 2020-01-16 | Luminex Corporation | Systems and methods for performing variable sample preparation and analysis processes |
| CN108823069A (zh) * | 2018-08-14 | 2018-11-16 | 南京唯诺尔科技项目管理有限公司 | 一种用于生物技术基因检测装置 |
| WO2020044688A1 (ja) * | 2018-08-28 | 2020-03-05 | 株式会社日立ハイテクノロジーズ | 自動分析装置 |
| CN109456876A (zh) * | 2018-11-15 | 2019-03-12 | 上海量能生物科技有限公司 | 带有搅拌功能的生物反应器 |
| CN109526305B (zh) * | 2018-11-21 | 2021-05-04 | 广东海洋大学 | 一种水稻耐盐碱性种质筛选用实验台 |
| CN109628441A (zh) * | 2018-12-07 | 2019-04-16 | 山东森芃生物科技有限公司 | 一种全自动核酸纯化仪 |
| CN109811038B (zh) * | 2019-01-17 | 2021-02-26 | 浙江大学 | 一种底照式核酸等温扩增检测便携仪器 |
| EP3918345A4 (en) * | 2019-01-28 | 2022-04-13 | Formulatrix, Inc. | LIQUID HANDLING INSTRUMENT AND PIPETTING HEAD FOR AND METHODS FOR Aspiration AND/OR DISPENSING OF LIQUIDS |
| CN109821585A (zh) * | 2019-02-19 | 2019-05-31 | 苏州奥素液芯电子科技有限公司 | 数字微流控系统中滴液加样或取样方法、移液器吸头组件 |
| US20220119799A1 (en) * | 2019-03-12 | 2022-04-21 | Hui Chen | Nucleic acid extraction apparatus |
| KR102266758B1 (ko) * | 2019-04-09 | 2021-06-18 | 주식회사 케이엠씨로보틱스 | 시료 분석용 전처리 자동화 장치 |
| GB201905196D0 (en) | 2019-04-12 | 2019-05-29 | Randox Laboratories Ltd | Sterilisation assembly |
| CN110146478B (zh) * | 2019-06-17 | 2020-03-24 | 滨州医学院 | 一种微生物培养皿的在线监测装置 |
| US12343722B2 (en) | 2019-07-29 | 2025-07-01 | Mgi Holdings Co., Limited | Reagent cartridges for in-vitro devices |
| WO2021074970A1 (ja) * | 2019-10-15 | 2021-04-22 | 株式会社日立ハイテク | 核酸試料処理装置および核酸試料処理方法 |
| CN110849701B (zh) * | 2019-11-04 | 2023-02-28 | 山东见微生物科技有限公司 | 样本处理设备 |
| CN216550412U (zh) | 2019-12-13 | 2022-05-17 | 贝克顿·迪金森公司 | 用于分析核酸的系统 |
| CN111154641B (zh) * | 2020-02-17 | 2024-08-13 | 广州市宝创生物技术有限公司 | 一种全自动核酸提取扩增检测仪 |
| CN111235008A (zh) * | 2020-02-29 | 2020-06-05 | 深圳市朗司医疗科技有限公司 | 一种封闭性一体化病原体检测卡盒 |
| EP4107247A2 (en) | 2020-03-10 | 2022-12-28 | Cellares Corporation | Systems, devices, and methods for cell processing |
| CN111534427A (zh) * | 2020-05-13 | 2020-08-14 | 深圳市飞梭医疗科技有限责任公司 | 一种全自动检测仪器 |
| KR102375818B1 (ko) * | 2020-05-28 | 2022-03-18 | 주식회사 디앤에이보이 | 검체 분석 장치 및 방법 |
| CN111624355A (zh) * | 2020-06-10 | 2020-09-04 | 波音特生物科技（南京）有限公司 | 一种胰岛素检测用易于大量筛查的诊断试剂盒 |
| KR102458032B1 (ko) * | 2020-06-16 | 2022-10-24 | 주식회사 에이아이바이오틱스 | Rt-pcr 디바이스 |
| KR102403439B1 (ko) * | 2020-07-16 | 2022-06-02 | 주식회사 에이아이바이오틱스 | 올인원 rt-pcr 장치 |
| WO2022068937A1 (zh) * | 2020-09-30 | 2022-04-07 | 富佳生技股份有限公司 | 核酸检测设备 |
| TWD218529S (zh) * | 2020-09-30 | 2022-05-01 | 富佳生技股份有限公司 | 核酸檢測儀之圖形化使用者介面 |
| KR20230066096A (ko) * | 2020-10-15 | 2023-05-12 | 주식회사 씨젠 | 용액 혼합 장치 및 용액 혼합 방법 |
| CN114276924B (zh) * | 2020-10-19 | 2023-12-19 | 成都瀚辰光翼生物工程有限公司 | 基因检测设备 |
| CN112175809A (zh) * | 2020-10-27 | 2021-01-05 | 广州和实生物技术有限公司 | 一体化核酸检测工作站 |
| EP4252908A4 (en) * | 2020-12-07 | 2025-02-19 | Bioneer Corporation | ADAPTER FOR MOUNTING A CONDUCTIVE PIPETTE, DEVICE FOR OPEN/CLOSING A SAMPLE TUBE AND AUTOMATIC SAMPLE ANALYSIS SYSTEM |
| KR102581063B1 (ko) * | 2020-12-31 | 2023-09-22 | 한국기계연구원 | 고속 온도제어가 가능한 고속 분자진단장치 및 이를 이용한 고속 분자진단방법 |
| KR102620858B1 (ko) * | 2021-04-29 | 2024-01-04 | 주식회사 제놀루션 | 핵산추출장치 및 핵산추출장치에 사용되는 캡 |
| KR102528711B1 (ko) * | 2021-06-02 | 2023-05-04 | 주식회사 어큐진 | 핵산추출장치 |
| CN113214982B (zh) * | 2021-06-09 | 2021-11-26 | 北京卓诚惠生生物科技股份有限公司 | 一种pcr腔室 |
| WO2023284844A1 (zh) * | 2021-07-15 | 2023-01-19 | 南京金斯瑞生物科技有限公司 | 一种自动提取设备及其使用方法 |
| CN116773841B (zh) * | 2022-03-08 | 2024-09-13 | 上海萨迦生物科技有限公司 | 一种基于激光共聚焦技术的全自动免疫分析仪及其检测方法 |
| KR102867764B1 (ko) * | 2022-04-01 | 2025-10-14 | 주식회사 앱솔로지 | 모듈 방식 기반의 체외진단장치 |
| CN114854533B (zh) * | 2022-05-24 | 2025-10-21 | 北京金豪制药股份有限公司 | 一种加热装置及核酸提取仪 |
| CN114736787A (zh) * | 2022-06-10 | 2022-07-12 | 至美时代生物智能科技(北京)有限公司 | 一种核酸洗脱与检测装置 |
| KR102674123B1 (ko) * | 2022-06-13 | 2024-06-12 | (주)옵토레인 | 검체 처리 장치 |
| CN115369016B (zh) * | 2022-07-29 | 2025-12-09 | 东莞市李群自动化技术有限公司 | 一种核酸检测装置及方法 |
| KR102786026B1 (ko) * | 2022-08-10 | 2025-03-27 | 주식회사 에이블랩스 | 자동 세포 분주 장치, 이를 포함하는 액체 핸들링 장치 및 자동 세포 분주 방법 |
| CN116081057A (zh) * | 2023-01-04 | 2023-05-09 | 天津迪安医学检验实验室有限公司 | 一种用于不同种试剂性能比对的矩阵式拼接式试剂盒 |
| KR102861226B1 (ko) * | 2023-02-28 | 2025-09-18 | (주)한국바이오셀프 | 액체생검을 위한 인체유래 물질 추출 자동화 기기 및 이를 이용한 인체유래 물질 추출 방법 |
| WO2024233080A1 (en) * | 2023-05-09 | 2024-11-14 | Cellares Corporation | Systems, devices, and methods for automated analysis of fluid samples from a cell processing system |
| KR102635367B1 (ko) * | 2023-05-23 | 2024-02-08 | 주식회사 제놀루션 | 검체처리장치 및 검체처리방법 |
| WO2025162375A1 (zh) * | 2024-02-02 | 2025-08-07 | 易孛特生命科学(上海)有限公司 | 提取仪 |
| WO2025171036A1 (en) * | 2024-02-06 | 2025-08-14 | Effani Agriculture, Inc. | Automation of biopsy analysis systems and methods |
| CN118726085A (zh) * | 2024-08-02 | 2024-10-01 | 安徽同科生物科技有限公司 | 一种用于pcr仪的进出仓式温控装置 |
Family Cites Families (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4988618A (en) * | 1987-11-16 | 1991-01-29 | Gene-Trak Systems | Magnetic separation device and methods for use in heterogeneous assays |
| EP0478753B1 (en) * | 1990-04-06 | 1997-07-02 | The Perkin-Elmer Corporation | Automated molecular biology laboratory |
| FI932866A0 (fi) | 1993-06-21 | 1993-06-21 | Labsystems Oy | Separeringsfoerfarande |
| JP3115501B2 (ja) | 1994-06-15 | 2000-12-11 | プレシジョン・システム・サイエンス株式会社 | 分注機を利用した磁性体の脱着制御方法及びこの方法によって処理される各種装置 |
| DE4423878A1 (de) | 1994-07-07 | 1996-01-11 | Boehringer Mannheim Gmbh | Vorrichtung und Verfahren zum Abscheiden von magnetischen Mikropartikeln |
| US7321828B2 (en) * | 1998-04-13 | 2008-01-22 | Isis Pharmaceuticals, Inc. | System of components for preparing oligonucleotides |
| JP3941391B2 (ja) * | 1998-06-04 | 2007-07-04 | 東洋鋼鈑株式会社 | 固定化ｄｎａライブラリー作製装置、遺伝子増幅装置、遺伝子増幅産物解析、遺伝子診断装置及びそれらの装置の制御方法 |
| US7799521B2 (en) * | 1998-06-24 | 2010-09-21 | Chen & Chen, Llc | Thermal cycling |
| US7138254B2 (en) * | 1999-08-02 | 2006-11-21 | Ge Healthcare (Sv) Corp. | Methods and apparatus for performing submicroliter reactions with nucleic acids or proteins |
| US6672458B2 (en) * | 2000-05-19 | 2004-01-06 | Becton, Dickinson And Company | System and method for manipulating magnetically responsive particles fluid samples to collect DNA or RNA from a sample |
| JP2003304899A (ja) * | 2002-04-11 | 2003-10-28 | Yoshinobu Baba | 核酸の分析方法及び装置 |
| CA2481899A1 (en) * | 2002-04-11 | 2003-10-23 | Infectio Diagnostic (I.D.I.) Inc. | Method for the preparation of reagents for amplification and/or detection of nucleic acids that exhibit no significant contamination by nucleic acids |
| KR100483684B1 (ko) * | 2003-01-29 | 2005-04-18 | (주)바이오넥스 | 핵산 또는 다양한 생물학적 물질을 분리 및 정제하기 위한키트와, 이러한 키트를 이용하여 생물학적 물질의 분리또는 정제 작업을 자동화하기 위한 시스템 |
| WO2004089545A1 (en) * | 2003-04-08 | 2004-10-21 | Auckland Uniservices Limited | Dna analysis system |
| JP2004317363A (ja) | 2003-04-17 | 2004-11-11 | Toyobo Co Ltd | 核酸分析装置、およびそれを用いた核酸の分析方法 |
| JP4736056B2 (ja) * | 2003-10-24 | 2011-07-27 | キアジェン サイエンシズ，リミティド ライアビリティ カンパニー | 統合された生物分析及びサンプル調製システム |
| KR101290558B1 (ko) * | 2004-07-30 | 2013-07-31 | 퓨리셀렉트 게엠베하 | 생물학적 연구를 포함하는 생명공학 및 의약적 진단학에서,동물에의 적용을 목적으로, 체액으로부터 세포, 생체 입자및/또는 분자를 분리하는 장치 및 방법 |
| KR100673811B1 (ko) * | 2005-05-11 | 2007-01-24 | 한국표준과학연구원 | 유전자 내 메틸 시토신의 정량 분석방법 및 이의 사용용도 |
| JP4619198B2 (ja) * | 2005-05-27 | 2011-01-26 | パナソニック株式会社 | 電気泳動システム |
| JP2007212310A (ja) * | 2006-02-09 | 2007-08-23 | Hitachi High-Tech Science Systems Corp | 微量液体試料分注装置 |
| US7998708B2 (en) * | 2006-03-24 | 2011-08-16 | Handylab, Inc. | Microfluidic system for amplifying and detecting polynucleotides in parallel |
| JP5038004B2 (ja) * | 2007-04-12 | 2012-10-03 | キヤノン株式会社 | 分析装置 |
| CN101990639B (zh) * | 2008-04-09 | 2014-08-13 | 株式会社百奥尼 | 自动提纯设备、多井板试样盒以及用于从生物试样提取核酸的方法 |
| KR101423936B1 (ko) * | 2009-03-11 | 2014-07-29 | (주)바이오니아 | 실시간 핵산 분석 통합 장치 및 이를 이용한 타겟 핵산의 검출방법 |
| EP2565260B1 (en) * | 2010-04-30 | 2020-12-23 | Bioneer Corporation | Automatic biological sample purification device having a magnetic-field-applying unit, a method for extracting a target substance from a biological sample, and a protein expression and purification method |
-
2012
- 2012-02-10 KR KR1020120013757A patent/KR101762295B1/ko not_active Expired - Fee Related
-
2013
- 2013-02-07 IN IN1572MUN2014 patent/IN2014MN01572A/en unknown
- 2013-02-07 WO PCT/KR2013/000985 patent/WO2013119049A1/ko not_active Ceased
- 2013-02-07 US US14/377,537 patent/US9765383B2/en not_active Expired - Fee Related
- 2013-02-07 JP JP2014556475A patent/JP5908613B2/ja not_active Expired - Fee Related
- 2013-02-07 CA CA2864260A patent/CA2864260C/en active Active
- 2013-02-07 AU AU2013218402A patent/AU2013218402B2/en not_active Ceased
- 2013-02-07 EP EP13746518.3A patent/EP2813579B1/en active Active
- 2013-02-07 BR BR112014019798-9A patent/BR112014019798B1/pt not_active IP Right Cessation
- 2013-02-07 CN CN201380018553.3A patent/CN104204229B/zh not_active Expired - Fee Related
- 2013-02-07 RU RU2014136713A patent/RU2610687C2/ru active
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|---|---|
| AU2013218402B2 (en) | 2016-07-07 |
| BR112014019798A8 (pt) | 2017-07-11 |
| BR112014019798A2 (pt) | 2021-06-01 |
| EP2813579A4 (en) | 2015-09-16 |
| AU2013218402A1 (en) | 2014-09-04 |
| EP2813579A1 (en) | 2014-12-17 |
| JP5908613B2 (ja) | 2016-04-26 |
| US20150037803A1 (en) | 2015-02-05 |
| WO2013119049A1 (ko) | 2013-08-15 |
| CA2864260A1 (en) | 2013-08-15 |
| RU2014136713A (ru) | 2016-04-10 |
| CA2864260C (en) | 2018-05-01 |
| CN104204229B (zh) | 2016-09-07 |
| IN2014MN01572A (enExample) | 2015-05-08 |
| JP2015506710A (ja) | 2015-03-05 |
| KR101762295B1 (ko) | 2017-08-04 |
| US9765383B2 (en) | 2017-09-19 |
| RU2610687C2 (ru) | 2017-02-14 |
| CN104204229A (zh) | 2014-12-10 |
| EP2813579B1 (en) | 2017-04-05 |
| KR20130092185A (ko) | 2013-08-20 |
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