BR112014018737A2 - método para a amplificação de ácidos nucleicos - Google Patents
método para a amplificação de ácidos nucleicosInfo
- Publication number
- BR112014018737A2 BR112014018737A2 BR112014018737A BR112014018737A BR112014018737A2 BR 112014018737 A2 BR112014018737 A2 BR 112014018737A2 BR 112014018737 A BR112014018737 A BR 112014018737A BR 112014018737 A BR112014018737 A BR 112014018737A BR 112014018737 A2 BR112014018737 A2 BR 112014018737A2
- Authority
- BR
- Brazil
- Prior art keywords
- nucleic acid
- acid amplification
- relates
- present
- nanoparticles
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
- B01L7/525—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
- B01L7/525—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones
- B01L7/5255—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones by moving sample containers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y20/00—Nanooptics, e.g. quantum optics or photonic crystals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1861—Means for temperature control using radiation
- B01L2300/1872—Infrared light
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Nanotechnology (AREA)
- Optics & Photonics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
resumo patente de invenção: "método para a amplificação de ácidos nucleicos". a presente invenção se refere a um método para a amplificação de ácidos nucleicos. particularmente, a presente invenção se refere a um método para a amplificação de ácidos nucleicos (1), em que as nanopartículas (8) em um volume de reação (2) transferem calor para seu ambiente através da agitação. 21935927v1
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE102012201475.6A DE102012201475B4 (de) | 2012-02-01 | 2012-02-01 | Verfahren zum Vervielfältigen von Nukleinsäuren |
PCT/EP2013/052100 WO2013113910A1 (en) | 2012-02-01 | 2013-02-01 | Method for the amplification of nucleic acids |
Publications (1)
Publication Number | Publication Date |
---|---|
BR112014018737A2 true BR112014018737A2 (pt) | 2017-07-04 |
Family
ID=47716001
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112014018737A BR112014018737A2 (pt) | 2012-02-01 | 2013-02-01 | método para a amplificação de ácidos nucleicos |
Country Status (14)
Country | Link |
---|---|
US (2) | US9382583B2 (pt) |
EP (2) | EP2809806B1 (pt) |
JP (2) | JP2015511124A (pt) |
CN (2) | CN107604052A (pt) |
AU (1) | AU2013214098B2 (pt) |
BR (1) | BR112014018737A2 (pt) |
CA (1) | CA2862912C (pt) |
DE (1) | DE102012201475B4 (pt) |
DK (1) | DK2809806T3 (pt) |
ES (1) | ES2596452T3 (pt) |
HU (1) | HUE030691T2 (pt) |
PL (1) | PL2809806T3 (pt) |
RU (1) | RU2603253C2 (pt) |
WO (1) | WO2013113910A1 (pt) |
Families Citing this family (22)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9816132B2 (en) | 2012-12-14 | 2017-11-14 | The Royal Institution For The Advancement Of Learning/Mcgill University | Heating mechanism for DNA amplification, extraction or sterilization using photo-thermal nanoparticles |
WO2015006864A1 (en) * | 2013-07-16 | 2015-01-22 | The Royal Institution For The Advancement Of Learning/Mcgill University | Heating mechanism for dna amplification, extraction or sterilization using photo-thermal nanoparticles |
DE102013022382B9 (de) * | 2013-08-01 | 2017-11-16 | Gna Biosolutions Gmbh | Verfahren zum Vervielfältigen von Nukleinsäuren |
DE102013215168B4 (de) | 2013-08-01 | 2016-01-07 | Gna Biosolutions Gmbh | Verwendung von einem oder mehreren Nanopartikeln zum Vervielfältigen von Nukleinsäuren |
DE102013215166B3 (de) * | 2013-08-01 | 2014-10-30 | Gna Biosolutions Gmbh | PCR-Verfahren zur Superamplifikation |
WO2015195357A1 (en) * | 2014-06-18 | 2015-12-23 | Luminex Corporation | Apparatus and methods for magnetic mixing |
US10544450B2 (en) | 2014-11-07 | 2020-01-28 | Gna Biosolutions Gmbh | Method for duplicating nucleic acids by means of a polymerase chain reaction |
US20180237824A1 (en) * | 2014-11-10 | 2018-08-23 | Gna Biosolutions Gmbh | Method for multiplying nucleic acids |
WO2016115542A1 (en) * | 2015-01-16 | 2016-07-21 | The Regents Of The University Of California | Led driven plasmonic heating apparatus for nucleic acids amplification |
WO2016200525A1 (en) * | 2015-06-08 | 2016-12-15 | The University Of Chicago | Bipyramid-templated synthesis of monodisperse noble metal nanocrystals |
DE102015010065B3 (de) * | 2015-08-03 | 2016-07-21 | Gna Biosolutions Gmbh | Verwendung von Nanopartikeln und von kationisch modifizierter Nukleinsäure zum Nachweis einer nachzuweisenden Nukleinsäure in einer Lösung |
EP3184647A1 (en) * | 2015-12-23 | 2017-06-28 | Koninklijke Philips N.V. | Laser pcr and bead detection in one reaction chamber |
DE102016120124B8 (de) | 2016-10-21 | 2018-08-23 | Gna Biosolutions Gmbh | Verfahren zum Durchführen einer Polymerase-Kettenreaktion und Vorrichtung zum Ausführen des Verfahrens |
DE102016124692B4 (de) | 2016-12-16 | 2019-05-16 | Gna Biosolutions Gmbh | Verfahren und System zum Vervielfältigen einer Nukleinsäure |
US11753485B2 (en) | 2017-09-26 | 2023-09-12 | National Research Council Of Canada | Microfluidic assisted fabrication of polymer microparticle-metal nanoparticle composites |
DE102017123919A1 (de) | 2017-10-13 | 2019-04-18 | Gna Biosolutions Gmbh | Verfahren und Vorrichtung zur Lyse von Mikroorganismen |
WO2019090306A1 (en) * | 2017-11-06 | 2019-05-09 | The Regents Of The University Of Michigan | Compositions and methods for detection of microorganisms |
DE102018103215B3 (de) | 2018-02-13 | 2019-08-14 | Gna Biosolutions Gmbh | Verfahren und Vorrichtung zur Extraktion einer Nukleinsäure aus einer Probenflüssigkeit |
EP3569717A3 (en) * | 2019-08-28 | 2020-04-08 | Boehringer Ingelheim Vetmedica GmbH | Diagnostic method for the detection of dna and rna viruses by carrying out a polymerase chain reaction in a heated reaction volume |
CN112301106A (zh) * | 2020-10-30 | 2021-02-02 | 南京师范大学 | 具有光热响应的DNA修饰的金纳米颗粒的DNA-AuNP探针及其制备方法和应用 |
CN113355455B (zh) * | 2021-05-21 | 2024-05-03 | 广东粤港澳大湾区国家纳米科技创新研究院 | 一种检测新型冠状病毒的荧光定量纳米粒子pcr的引物、引物探针组合及其应用 |
CN113278733B (zh) * | 2021-05-21 | 2024-03-01 | 广东粤港澳大湾区国家纳米科技创新研究院 | 一种检测新冠病毒的突变毒株的引物和探针的组合 |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US6953659B2 (en) * | 2000-07-14 | 2005-10-11 | Massachusetts Institute Of Technology | Direct, externally imposed control of nucleic acids |
EP1356086A2 (de) | 2000-12-13 | 2003-10-29 | Institut Für Physikalische Hochtechnologie E.V. | Verfahren zum simultanen positionsspezifischen schneiden von fadenförmigen organischen molekülketten, insbesonere dna |
WO2003048769A1 (en) | 2001-11-30 | 2003-06-12 | Nanosphere, Inc. | Real-time monitoring of pcr amplification using nanoparticle probes |
WO2005042783A1 (en) * | 2003-10-30 | 2005-05-12 | North Carolina State University | Temperature-jump enhanced electrochemical detection of nucleic acid hybridization |
EP1840222B1 (en) * | 2004-12-29 | 2011-02-23 | Suzhou Yangtze Delta Academy of Bio-x Science Ltd. | The optimum method of amplification on polymerase chain reaction |
EP2029721A4 (en) | 2006-05-30 | 2011-01-26 | Univ Utah Res Found | SIMULTANEOUS AMPLIFICATION AND DETECTION OF RIBONUCLEIC ACID BY AN OPTICAL METHOD USING RESONANCE OF SURFACE PLASMONS |
DE102007027654B8 (de) * | 2007-06-15 | 2012-03-15 | Gna Biosolutions Gmbh | Verfahren zum Nachweis von Nukleinsäuren |
EP2110175A1 (de) * | 2008-03-20 | 2009-10-21 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Verfahren und Vorrichtung zur thermischen Steuerung biologischer und chemischer Reaktionen unter Einsatz von magnetischen Partikeln oder magnetischen Beads und Wechselmagnetfeldern |
-
2012
- 2012-02-01 DE DE102012201475.6A patent/DE102012201475B4/de active Active
-
2013
- 2013-02-01 JP JP2014555234A patent/JP2015511124A/ja not_active Withdrawn
- 2013-02-01 CN CN201710868078.9A patent/CN107604052A/zh active Pending
- 2013-02-01 PL PL13704388T patent/PL2809806T3/pl unknown
- 2013-02-01 CN CN201380007616.5A patent/CN104093860A/zh active Pending
- 2013-02-01 AU AU2013214098A patent/AU2013214098B2/en not_active Ceased
- 2013-02-01 EP EP13704388.1A patent/EP2809806B1/en active Active
- 2013-02-01 DK DK13704388.1T patent/DK2809806T3/en active
- 2013-02-01 RU RU2014135391/10A patent/RU2603253C2/ru active
- 2013-02-01 ES ES13704388.1T patent/ES2596452T3/es active Active
- 2013-02-01 EP EP16179482.1A patent/EP3101143B1/en active Active
- 2013-02-01 HU HUE13704388A patent/HUE030691T2/en unknown
- 2013-02-01 BR BR112014018737A patent/BR112014018737A2/pt not_active Application Discontinuation
- 2013-02-01 US US14/375,946 patent/US9382583B2/en active Active
- 2013-02-01 CA CA2862912A patent/CA2862912C/en active Active
- 2013-02-01 WO PCT/EP2013/052100 patent/WO2013113910A1/en active Application Filing
-
2016
- 2016-01-25 JP JP2016011619A patent/JP6475645B2/ja not_active Expired - Fee Related
- 2016-06-07 US US15/175,237 patent/US9926594B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
HUE030691T2 (en) | 2017-05-29 |
EP3101143B1 (en) | 2018-08-01 |
EP2809806A1 (en) | 2014-12-10 |
RU2014135391A (ru) | 2016-03-27 |
US20140377764A1 (en) | 2014-12-25 |
ES2596452T3 (es) | 2017-01-09 |
JP2016127842A (ja) | 2016-07-14 |
DE102012201475A1 (de) | 2013-08-01 |
AU2013214098A1 (en) | 2014-07-24 |
JP6475645B2 (ja) | 2019-02-27 |
US20160281139A1 (en) | 2016-09-29 |
DK2809806T3 (en) | 2016-10-10 |
EP2809806B1 (en) | 2016-08-10 |
WO2013113910A1 (en) | 2013-08-08 |
CN107604052A (zh) | 2018-01-19 |
DE102012201475B4 (de) | 2014-12-18 |
AU2013214098B2 (en) | 2016-05-12 |
CA2862912C (en) | 2017-02-28 |
US9382583B2 (en) | 2016-07-05 |
EP3101143A1 (en) | 2016-12-07 |
CA2862912A1 (en) | 2013-08-08 |
US9926594B2 (en) | 2018-03-27 |
RU2603253C2 (ru) | 2016-11-27 |
CN104093860A (zh) | 2014-10-08 |
JP2015511124A (ja) | 2015-04-16 |
PL2809806T3 (pl) | 2017-02-28 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] | ||
B06U | Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette] | ||
B06A | Patent application procedure suspended [chapter 6.1 patent gazette] | ||
B07A | Application suspended after technical examination (opinion) [chapter 7.1 patent gazette] | ||
B09B | Patent application refused [chapter 9.2 patent gazette] | ||
B09B | Patent application refused [chapter 9.2 patent gazette] |
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