BR112012029769A2 - processo para a detecção eletroquímica de reações de ligação - Google Patents

processo para a detecção eletroquímica de reações de ligação

Info

Publication number
BR112012029769A2
BR112012029769A2 BR112012029769A BR112012029769A BR112012029769A2 BR 112012029769 A2 BR112012029769 A2 BR 112012029769A2 BR 112012029769 A BR112012029769 A BR 112012029769A BR 112012029769 A BR112012029769 A BR 112012029769A BR 112012029769 A2 BR112012029769 A2 BR 112012029769A2
Authority
BR
Brazil
Prior art keywords
electrochemical detection
binding reactions
automated
specifically binds
microfluidic
Prior art date
Application number
BR112012029769A
Other languages
English (en)
Other versions
BR112012029769B1 (pt
Inventor
Micheel Burkhard
Scharte Gudrun
Ettlinger Julia
Schenk Joerg
Gajovic Eichelmann Nenad
Original Assignee
Fraunhofer Ges Forschung
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fraunhofer Ges Forschung filed Critical Fraunhofer Ges Forschung
Publication of BR112012029769A2 publication Critical patent/BR112012029769A2/pt
Publication of BR112012029769B1 publication Critical patent/BR112012029769B1/pt

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
    • G01N27/3277—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction being a redox reaction, e.g. detection by cyclic voltammetry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G01N33/5438—Electrodes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • G01N33/743—Steroid hormones

Abstract

processo para a detecção eletroquímica de reações de ligação. a presente invenção refere-se a um processo para a realização de formatos de imunoensaios homogêneos com detecção eletroquímica altamente sensível em solução, caracterizado pelo fato de que dois diferentes conjugados como reagentes são combinados com a amostra ou com uma mistura de tampão-amostra, sendo que o primeiro conjugado compreende um marcador redox e uma molécula de analito e o segundo conjugado compreende um anticorpo antimarcador redox ou um fragmento deste que liga especificamente e uma molécula que liga especificamente o analito. o processo é adequado para imunoensaios livres de lavagem e separação no diagnóstico, especialmente para imunoensaios automatizados e microfluídicos. o processo também é adequado para sistemas de imunoensaios automatizados e microfluídicos. o processo também é adequado para sistemas de imunoensaios econômicos e tiras de teste para uso descartável.
BR112012029769A 2010-05-25 2011-05-25 processo para realização de formatos de imunoensaios homogêneos competitivos com a detecção eletroquímica em solução BR112012029769B1 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP10005425.3A EP2390664B1 (de) 2010-05-25 2010-05-25 Verfahren zur elektrochemischen Detektion von Bindungsreaktionen
PCT/EP2011/002586 WO2011147563A1 (de) 2010-05-25 2011-05-25 Verfahren zur elektrochemischen detektion von bindungsreaktionen

Publications (2)

Publication Number Publication Date
BR112012029769A2 true BR112012029769A2 (pt) 2017-06-27
BR112012029769B1 BR112012029769B1 (pt) 2019-12-24

Family

ID=42340815

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112012029769A BR112012029769B1 (pt) 2010-05-25 2011-05-25 processo para realização de formatos de imunoensaios homogêneos competitivos com a detecção eletroquímica em solução

Country Status (8)

Country Link
US (1) US8945852B2 (pt)
EP (1) EP2390664B1 (pt)
JP (1) JP5781603B2 (pt)
CN (1) CN102959397B (pt)
BR (1) BR112012029769B1 (pt)
DK (1) DK2390664T3 (pt)
ES (1) ES2410754T3 (pt)
WO (1) WO2011147563A1 (pt)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2657347A1 (en) 2012-04-26 2013-10-30 Université Paris Diderot - Paris 7 Electrochemical competitive assay and use thereof
US11009479B2 (en) 2014-05-27 2021-05-18 Case Western Reserve University Systems and methods for the detection of HbA1c
WO2018183759A1 (en) * 2017-03-29 2018-10-04 Case Western Reserve University COMPOSITIONS AND METHODS OF DETECTING 17 β-ESTRADIOL
JP7112966B2 (ja) * 2016-06-30 2022-08-04 シーメンス ヘルシニアーズ ネイザランド ビー．ブイ． 複数の細胞を含む体液試料内で分析物を検出するための装置、システム及び方法
WO2018112458A1 (en) * 2016-12-16 2018-06-21 Infusense Corp. Compositions and methods to detect molecules in a sample
JP7339245B2 (ja) * 2017-06-12 2023-09-05 エッセンリックス コーポレーション 均質アッセイ法
US20210364506A1 (en) * 2020-05-22 2021-11-25 Robert Bosch Gmbh Methods for detecting an amount of an analyte in a solution
KR20220104491A (ko) * 2021-01-18 2022-07-26 (주)옵토레인 스위칭 펩티드 및 이를 이용한 면역분석 방법

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL154599B (nl) 1970-12-28 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen, alsmede testverpakking.
US4016043A (en) 1975-09-04 1977-04-05 Akzona Incorporated Enzymatic immunological method for the determination of antigens and antibodies
EP0200960A1 (en) 1985-05-08 1986-11-12 Abbott Laboratories Total estriol fluorescence polarization immunoassay
EP0274343A1 (en) 1987-01-06 1988-07-13 IntraCel Corporation A system of reactants involving an apo-enzyme useful in immunological analysis and a method for carrying out immunoassays with this system
US5198367A (en) * 1989-06-09 1993-03-30 Masuo Aizawa Homogeneous amperometric immunoassay
IL116921A (en) * 1996-01-26 2000-11-21 Yissum Res Dev Co Electrochemical system for determination of an analyte in a liquid medium
JPH09257793A (ja) * 1996-03-27 1997-10-03 Kagaku Gijutsu Shinko Jigyodan 免疫反応測定用抗体とこれを用いた電気化学的測定方 法
GB2322192B (en) 1997-02-14 2001-01-31 Unilever Plc Assay devices
JP2002510032A (ja) * 1997-11-21 2002-04-02 ユニリーバー・ナームローゼ・ベンノートシヤープ 電気化学的アッセイの改良又は電気化学的アッセイに関する改良
RU2161653C2 (ru) * 1998-08-24 2001-01-10 ФАРМАКОВСКИЙ Дмитрий Александрович Способ количественного электрохимического анализа биомолекул
EP1331482A1 (de) 2002-01-25 2003-07-30 BMS Sensor Technology SA Konjugat zur Bestimmung von Antigenen mittels Biosensoren
CN100392385C (zh) * 2002-12-03 2008-06-04 博奥生物有限公司 亲和反应的化学放大电化学检测方法及其试剂盒
EP1646637B1 (en) 2003-07-01 2012-08-15 Roche Diagnostics GmbH Electrochemical affinity biosensor system and methods
GB0428130D0 (en) * 2004-12-22 2005-01-26 Oxford Biosensors Ltd Selective HDL cholesterol assay
CN101184996A (zh) * 2005-03-31 2008-05-21 因韦尔尼斯医药瑞士股份有限公司 电化学检测法
DE102005020384A1 (de) 2005-05-02 2006-11-09 Therainvention Gmbh Spektroskopisches Verfahren zum Nachweis von Analyten
WO2007123507A1 (en) * 2006-03-20 2007-11-01 Inverness Medical Switzerland Gmbh Water-soluble conjugates for electrochemical detection
DE102008027038A1 (de) * 2008-06-06 2009-12-17 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Verfahren zum Detektieren von chemischen oder biologischen Species sowie Elektrodenanordnung hierfür

Also Published As

Publication number Publication date
ES2410754T3 (es) 2013-07-03
EP2390664B1 (de) 2013-04-17
JP5781603B2 (ja) 2015-09-24
CN102959397B (zh) 2014-12-31
US20130203065A1 (en) 2013-08-08
US8945852B2 (en) 2015-02-03
DK2390664T3 (da) 2013-07-22
WO2011147563A1 (de) 2011-12-01
WO2011147563A8 (de) 2012-12-06
CN102959397A (zh) 2013-03-06
EP2390664A1 (de) 2011-11-30
WO2011147563A9 (de) 2013-01-24
JP2013526720A (ja) 2013-06-24
BR112012029769B1 (pt) 2019-12-24

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Legal Events

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B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06T Formal requirements before examination [chapter 6.20 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 25/05/2011, OBSERVADAS AS CONDICOES LEGAIS.