CN100392385C - 亲和反应的化学放大电化学检测方法及其试剂盒 - Google Patents
亲和反应的化学放大电化学检测方法及其试剂盒 Download PDFInfo
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Images
Classifications
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- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
- G01N33/5438—Electrodes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
- G01N27/3277—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction being a redox reaction, e.g. detection by cyclic voltammetry
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6825—Nucleic acid detection involving sensors
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2458/00—Labels used in chemical analysis of biological material
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
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- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
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- Biotechnology (AREA)
- Microbiology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Electrochemistry (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
还原剂 | 放大系数 |
草酸钠(pH 5.5) | 2800 |
甲酸 | 1 |
三乙胺 | <238 |
三丙胺 | 238 |
三丁胺 | <238 |
脯氨酸 | 580 |
HEPES(pH 8.5) | 550 |
PIPES(pH 8.5) | 175 |
鸟嘌呤核苷 | <<238 |
Claims (35)
Priority Applications (7)
Application Number | Priority Date | Filing Date | Title |
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CNB021536651A CN100392385C (zh) | 2002-12-03 | 2002-12-03 | 亲和反应的化学放大电化学检测方法及其试剂盒 |
EP03729794A EP1576369A4 (en) | 2002-12-03 | 2003-05-06 | CHEMICALLY REINFORCED ELECTROCHEMICAL DETECTION OF AN AFFINITY REACTION |
CA002508212A CA2508212A1 (en) | 2002-12-03 | 2003-05-06 | Chemically amplified electrochemical detection of affinity reaction |
JP2004555956A JP2006508351A (ja) | 2002-12-03 | 2003-05-06 | 親和性反応の化学的に増幅された電気化学的検出 |
AU2003240378A AU2003240378A1 (en) | 2002-12-03 | 2003-05-06 | Chemically amplified electrochemical detection of affinity reaction |
US10/537,378 US20060134608A1 (en) | 2002-12-03 | 2003-05-06 | Chemically amplified electrochemical detection of affinity reaction |
PCT/CN2003/000329 WO2004051274A1 (en) | 2002-12-03 | 2003-05-06 | Ahemically amplified electrochemical detection of affinity reaction |
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Application Number | Priority Date | Filing Date | Title |
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CNB021536651A CN100392385C (zh) | 2002-12-03 | 2002-12-03 | 亲和反应的化学放大电化学检测方法及其试剂盒 |
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CN1504746A CN1504746A (zh) | 2004-06-16 |
CN100392385C true CN100392385C (zh) | 2008-06-04 |
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US (1) | US20060134608A1 (zh) |
EP (1) | EP1576369A4 (zh) |
JP (1) | JP2006508351A (zh) |
CN (1) | CN100392385C (zh) |
AU (1) | AU2003240378A1 (zh) |
CA (1) | CA2508212A1 (zh) |
WO (1) | WO2004051274A1 (zh) |
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EP2061572A1 (en) | 2006-08-31 | 2009-05-27 | Technion Research & Development Foundation Ltd. | Controllable binding and dissociation of chemical entities and electrode devices therefore |
WO2011063395A2 (en) | 2009-11-23 | 2011-05-26 | The Johns Hopkins University | Lateral flow device for diagnosing microbial infections |
US10585098B2 (en) | 2009-11-23 | 2020-03-10 | The Johns Hopkins University | Optimizing diagnostics for galactofuranose containing antigens |
JP5311410B2 (ja) * | 2009-12-25 | 2013-10-09 | 独立行政法人産業技術総合研究所 | 酸化還元物質の検出における感度増感方法及びそのための装置 |
DK2390664T3 (da) * | 2010-05-25 | 2013-07-22 | Fraunhofer Ges Forschung | Fremgangsmåde til elektrokemisk påvisning af bindingsreaktioner |
US10107822B2 (en) | 2013-12-16 | 2018-10-23 | The Johns Hopkins University | Interferon-gamma release assays for diagnosis of invasive fungal infections |
WO2017083245A1 (en) * | 2015-11-10 | 2017-05-18 | Saint Louis University | Universal bioelectrochemical metabolic flux measurement system and methods of making and using the same |
CN107228885B (zh) * | 2017-06-29 | 2020-06-26 | 江苏大学 | 一种色素纳米囊泡仿生气体传感器的制备方法 |
CA3141294A1 (en) * | 2019-05-28 | 2020-12-03 | Ramot At Tel-Aviv University Ltd. | Systems and methods for detecting a pathogenic organism |
GB2608318A (en) * | 2020-03-13 | 2022-12-28 | Apton Biosystems Inc | Densely-packed analyte layers and detection methods |
Citations (5)
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---|---|---|---|---|
US5312527A (en) * | 1992-10-06 | 1994-05-17 | Concordia University | Voltammetric sequence-selective sensor for target polynucleotide sequences |
CN1026248C (zh) * | 1990-02-03 | 1994-10-19 | 曼海姆泊灵格股份公司 | 电化学测定被分析物和氧化还原酶的方法和敏感电极系统 |
CN1249815A (zh) * | 1997-02-06 | 2000-04-05 | 查珀尔希尔北卡罗来纳大学 | 用于分子相互作用的检测和药物发现的电化学探针 |
US6221586B1 (en) * | 1997-04-09 | 2001-04-24 | California Institute Of Technology | Electrochemical sensor using intercalative, redox-active moieties |
US6277576B1 (en) * | 1993-12-10 | 2001-08-21 | California Institute Of Technology | Nucleic acid mediated electron transfer |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
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GB9225354D0 (en) * | 1992-12-04 | 1993-01-27 | Univ Manchester | Immunoassay |
US5871918A (en) * | 1996-06-20 | 1999-02-16 | The University Of North Carolina At Chapel Hill | Electrochemical detection of nucleic acid hybridization |
US6346387B1 (en) * | 1995-06-27 | 2002-02-12 | Xanthon, Inc. | Detection of binding reactions using labels detected by mediated catalytic electrochemistry |
RU2161653C2 (ru) * | 1998-08-24 | 2001-01-10 | ФАРМАКОВСКИЙ Дмитрий Александрович | Способ количественного электрохимического анализа биомолекул |
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Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1026248C (zh) * | 1990-02-03 | 1994-10-19 | 曼海姆泊灵格股份公司 | 电化学测定被分析物和氧化还原酶的方法和敏感电极系统 |
US5312527A (en) * | 1992-10-06 | 1994-05-17 | Concordia University | Voltammetric sequence-selective sensor for target polynucleotide sequences |
US6277576B1 (en) * | 1993-12-10 | 2001-08-21 | California Institute Of Technology | Nucleic acid mediated electron transfer |
CN1249815A (zh) * | 1997-02-06 | 2000-04-05 | 查珀尔希尔北卡罗来纳大学 | 用于分子相互作用的检测和药物发现的电化学探针 |
US6221586B1 (en) * | 1997-04-09 | 2001-04-24 | California Institute Of Technology | Electrochemical sensor using intercalative, redox-active moieties |
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AU2003240378A1 (en) | 2004-06-23 |
JP2006508351A (ja) | 2006-03-09 |
CN1504746A (zh) | 2004-06-16 |
CA2508212A1 (en) | 2004-06-17 |
WO2004051274A1 (en) | 2004-06-17 |
EP1576369A4 (en) | 2007-02-28 |
US20060134608A1 (en) | 2006-06-22 |
EP1576369A1 (en) | 2005-09-21 |
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