BR112012015596B1 - Processo de cultura de células aderentes - Google Patents

Processo de cultura de células aderentes Download PDF

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Publication number
BR112012015596B1
BR112012015596B1 BR112012015596-2A BR112012015596A BR112012015596B1 BR 112012015596 B1 BR112012015596 B1 BR 112012015596B1 BR 112012015596 A BR112012015596 A BR 112012015596A BR 112012015596 B1 BR112012015596 B1 BR 112012015596B1
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BR
Brazil
Prior art keywords
cell
cells
microcarriers
culture
culture medium
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BR112012015596-2A
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English (en)
Portuguese (pt)
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BR112012015596A2 (pt
Inventor
Eric Calvosa
Nicolas Seve
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Sanofi Pasteur
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Priority claimed from FR1051754A external-priority patent/FR2957355B1/fr
Application filed by Sanofi Pasteur filed Critical Sanofi Pasteur
Publication of BR112012015596A2 publication Critical patent/BR112012015596A2/pt
Publication of BR112012015596B1 publication Critical patent/BR112012015596B1/pt

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018Culture media for cell or tissue culture
    • C12N5/0056Xeno-free medium
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12Antivirals
    • A61P31/14Antivirals for RNA viruses
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068General culture methods using substrates
    • C12N5/0075General culture methods using substrates using microcarriers
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
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    • C12N7/00Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
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    • C12N2500/00Specific components of cell culture medium
    • C12N2500/90Serum-free medium, which may still contain naturally-sourced components
    • C12N2500/92Medium free of human- or animal-derived components
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    • C12N2710/00011Details
    • C12N2710/16011Herpesviridae
    • C12N2710/16711Varicellovirus, e.g. human herpesvirus 3, Varicella Zoster, pseudorabies
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    • C12N2760/00011Details
    • C12N2760/20011Rhabdoviridae
    • C12N2760/20111Lyssavirus, e.g. rabies virus
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    • C12N2760/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011Details
    • C12N2760/20011Rhabdoviridae
    • C12N2760/20111Lyssavirus, e.g. rabies virus
    • C12N2760/20151Methods of production or purification of viral material

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
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  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Communicable Diseases (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Oncology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
BR112012015596-2A 2009-12-23 2010-12-21 Processo de cultura de células aderentes BR112012015596B1 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
FR0959472 2009-12-23
FR0959472 2009-12-23
FR1051754A FR2957355B1 (fr) 2010-03-11 2010-03-11 Procede de culture de cellules adherentes a des microporteurs
FR1051754 2010-03-11
PCT/FR2010/052850 WO2011077035A1 (fr) 2009-12-23 2010-12-21 Procede de culture de cellules adherentes

Publications (2)

Publication Number Publication Date
BR112012015596A2 BR112012015596A2 (pt) 2015-11-03
BR112012015596B1 true BR112012015596B1 (pt) 2021-06-01

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
BR112012015596-2A BR112012015596B1 (pt) 2009-12-23 2010-12-21 Processo de cultura de células aderentes

Country Status (14)

Country Link
US (1) US9145543B2 (enExample)
EP (1) EP2516623B1 (enExample)
JP (1) JP5894929B2 (enExample)
KR (1) KR101835100B1 (enExample)
CN (2) CN102782121A (enExample)
AR (1) AR079710A1 (enExample)
AU (1) AU2010334723C1 (enExample)
BR (1) BR112012015596B1 (enExample)
CA (1) CA2784741A1 (enExample)
HK (1) HK1243731A1 (enExample)
IL (1) IL220167B (enExample)
MX (1) MX2012007117A (enExample)
RU (1) RU2612154C2 (enExample)
WO (1) WO2011077035A1 (enExample)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140271633A1 (en) * 2013-03-14 2014-09-18 Abbvie Inc. Mammalian cell culture performance through surfactant supplementation of feed media
CN103289949A (zh) * 2013-06-28 2013-09-11 四川省华派生物制药有限公司 一种从克氏瓶直接到生物反应器的细胞培养方法
EP3778702B1 (en) 2014-03-25 2023-06-28 F. Hoffmann-La Roche AG Methods of preparing a poloxamer for use in cell culture medium
ES2909630T3 (es) * 2014-06-04 2022-05-09 Amgen Inc Métodos de recolección en cultivos de células de mamífero
EP3475410A4 (en) 2016-06-24 2020-02-12 Lonza Ltd VARIABLE DIAMETER BIOREACTORS
CN106754672A (zh) * 2016-11-30 2017-05-31 广州赛莱拉干细胞科技股份有限公司 一种贴壁细胞的培养方法
CA3103570A1 (en) * 2018-06-14 2019-12-19 Abraham J And Phyllis Katz Cord Blood Foundation Isolation of mesenchymal stromal cells from umbilical cord blood
WO2021181819A1 (ja) * 2020-03-13 2021-09-16 昭和電工マテリアルズ株式会社 細胞懸濁液の製造方法、及び、接着細胞の製造方法
BR112022024982A2 (pt) * 2020-06-12 2022-12-27 Biotech Foods S L Processo e dispositivo para produção de carne de cultura
CN116036257B (zh) * 2022-12-29 2024-11-15 瑞普(保定)生物药业有限公司 一种猪瘟活疫苗的制备方法
WO2025125314A1 (fr) 2023-12-11 2025-06-19 Commissariat A L'energie Atomique Et Aux Energies Alternatives Dispositif et procédé de culture cellulaire

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4664912A (en) 1984-10-01 1987-05-12 Wiktor Tadeusz J Process for the large scale production of rabies vaccine
FR2635008B1 (fr) 1988-08-05 1990-11-16 Sanofi Sa Agent activateur de la productivite specifique des cellules animales recombinantes a base de polyvinylpyrrolidone et milieu de culture defini le contenant
DK52393D0 (enExample) 1993-05-05 1993-05-05 Novo Nordisk As
US5714375A (en) * 1995-06-05 1998-02-03 Nobl Laboratories, Inc. Ileal symbiont intracellularis propagation in suspended host cells
ATE234631T1 (de) * 1996-07-12 2003-04-15 Aventis Pasteur Immunisierungsverfahren in zwei schritten gegen pyramyxoviridae-viren unter verwendung abgeschwächter viraler stämme und preparation von proteinuntereinheiten
US6020182A (en) * 1996-07-12 2000-02-01 Connaught Laboratories Limited Subunit respiratory syncytial virus vaccine preparation
US6168944B1 (en) * 1997-01-31 2001-01-02 Schering Corporation Methods for cultivating cells and propagating viruses
SK285971B6 (sk) 1997-12-24 2007-12-06 Duphar International Research B. V. Spôsob prípravy buniek na výrobu biologických materiálov
FR2801900B1 (fr) 1999-12-03 2004-02-27 Pasteur Merieux Serums Vacc Methode de production de cellules animales adherentes
US6670184B2 (en) * 2000-06-23 2003-12-30 Basf Ag Method for producing a lipid emulsion for use in insect cell culture
US6951752B2 (en) * 2001-12-10 2005-10-04 Bexter Healthcare S.A. Method for large scale production of virus antigen
US6855535B2 (en) * 2001-12-10 2005-02-15 Baxter Healthcare S.A. Method of large scale production of Hepatitis A virus
DE102004043256B4 (de) * 2004-09-07 2013-09-19 Rheinische Friedrich-Wilhelms-Universität Bonn Skalierbarer Prozess zur Kultivierung undifferenzierter Stammzellen in Suspension
CN101265464B (zh) * 2008-05-13 2010-06-16 中国人民解放军第二军医大学 一种猪骨髓内皮祖细胞的分离和培养方法

Also Published As

Publication number Publication date
HK1243731A1 (zh) 2018-07-20
AU2010334723A1 (en) 2012-07-12
CN107460154A (zh) 2017-12-12
WO2011077035A1 (fr) 2011-06-30
CA2784741A1 (fr) 2011-06-30
BR112012015596A2 (pt) 2015-11-03
KR101835100B1 (ko) 2018-03-08
RU2012130928A (ru) 2014-01-27
MX2012007117A (es) 2012-07-23
AR079710A1 (es) 2012-02-15
IL220167A0 (en) 2012-07-31
US20110151506A1 (en) 2011-06-23
EP2516623A1 (fr) 2012-10-31
JP2013515473A (ja) 2013-05-09
US9145543B2 (en) 2015-09-29
RU2612154C2 (ru) 2017-03-02
JP5894929B2 (ja) 2016-03-30
EP2516623B1 (fr) 2016-10-05
AU2010334723B2 (en) 2015-02-05
KR20120102788A (ko) 2012-09-18
CN102782121A (zh) 2012-11-14
AU2010334723C1 (en) 2015-05-28
IL220167B (en) 2018-01-31

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Free format text: DE ACORDO COM O ARTIGO 229-C DA LEI NO 10196/2001, QUE MODIFICOU A LEI NO 9279/96, A CONCESSAO DA PATENTE ESTA CONDICIONADA A ANUENCIA PREVIA DA ANVISA. CONSIDERANDO A APROVACAO DOS TERMOS DO PARECER NO 337/PGF/EA/2010, BEM COMO A PORTARIA INTERMINISTERIAL NO 1065 DE 24/05/2012, ENCAMINHA-SE O PRESENTE PEDIDO PARA AS PROVIDENCIAS CABIVEIS.

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Owner name: SANOFI PASTEUR (FR)

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