BR112012002137B8 - método para produzir um polipeptídeo e/ou vírus de interesse em uma cultura de célula contínua isenta de soro - Google Patents
método para produzir um polipeptídeo e/ou vírus de interesse em uma cultura de célula contínua isenta de soro Download PDFInfo
- Publication number
- BR112012002137B8 BR112012002137B8 BR112012002137A BR112012002137A BR112012002137B8 BR 112012002137 B8 BR112012002137 B8 BR 112012002137B8 BR 112012002137 A BR112012002137 A BR 112012002137A BR 112012002137 A BR112012002137 A BR 112012002137A BR 112012002137 B8 BR112012002137 B8 BR 112012002137B8
- Authority
- BR
- Brazil
- Prior art keywords
- cell culture
- polypeptide
- virus
- interest
- culture system
- Prior art date
Links
- 238000004113 cell culture Methods 0.000 title abstract 9
- 241000700605 Viruses Species 0.000 title abstract 5
- 229920001184 polypeptide Polymers 0.000 title abstract 5
- 102000004196 processed proteins & peptides Human genes 0.000 title abstract 5
- 108090000765 processed proteins & peptides Proteins 0.000 title abstract 5
- 238000004519 manufacturing process Methods 0.000 title abstract 3
- 210000004027 cell Anatomy 0.000 abstract 7
- 210000004962 mammalian cell Anatomy 0.000 abstract 3
- 238000010790 dilution Methods 0.000 abstract 2
- 239000012895 dilution Substances 0.000 abstract 2
- 230000014759 maintenance of location Effects 0.000 abstract 2
- 238000012258 culturing Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 230000010412 perfusion Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
- C12M3/006—Cell injection or fusion devices
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
- C12N5/0056—Xeno-free medium
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6489—Metalloendopeptidases (3.4.24)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/98—Xeno-free medium and culture conditions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/00051—Methods of production or purification of viral material
- C12N2710/00052—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/24—Metalloendopeptidases (3.4.24)
- C12Y304/24087—ADAMTS13 endopeptidase (3.4.24.87)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Virology (AREA)
- Immunology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Cell Biology (AREA)
- Sustainable Development (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US23031309P | 2009-07-31 | 2009-07-31 | |
| PCT/EP2010/061191 WO2011012725A1 (en) | 2009-07-31 | 2010-08-02 | Method of producing a polypeptide or virus of interest in a continuous cell culture |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| BR112012002137A2 BR112012002137A2 (pt) | 2015-09-15 |
| BR112012002137B1 BR112012002137B1 (pt) | 2020-04-14 |
| BR112012002137B8 true BR112012002137B8 (pt) | 2020-05-12 |
Family
ID=42668436
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112012002137A BR112012002137B8 (pt) | 2009-07-31 | 2010-08-02 | método para produzir um polipeptídeo e/ou vírus de interesse em uma cultura de célula contínua isenta de soro |
Country Status (22)
| Country | Link |
|---|---|
| US (4) | US8580554B2 (https=) |
| EP (2) | EP2459697B1 (https=) |
| JP (2) | JP5808742B2 (https=) |
| KR (2) | KR101885046B1 (https=) |
| CN (1) | CN102549142B (https=) |
| AU (1) | AU2010277490B2 (https=) |
| BR (1) | BR112012002137B8 (https=) |
| CA (1) | CA2769354A1 (https=) |
| CO (1) | CO6491119A2 (https=) |
| DK (1) | DK2459697T3 (https=) |
| EA (1) | EA022219B1 (https=) |
| ES (1) | ES2705206T3 (https=) |
| HR (1) | HRP20182165T1 (https=) |
| IN (1) | IN2012DN00899A (https=) |
| MX (2) | MX2012001274A (https=) |
| NZ (1) | NZ597742A (https=) |
| PL (1) | PL2459697T3 (https=) |
| PT (1) | PT2459697T (https=) |
| SG (1) | SG178176A1 (https=) |
| SI (1) | SI2459697T1 (https=) |
| TR (1) | TR201900213T4 (https=) |
| WO (1) | WO2011012725A1 (https=) |
Families Citing this family (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012006594A1 (en) | 2010-07-08 | 2012-01-12 | Baxter International Inc. | Method of producing recombinant adamts13 in cell culture |
| EP4036207B1 (en) * | 2011-01-17 | 2025-10-22 | F. Hoffmann-La Roche AG | Use of a separation apparatus in a seed train |
| ES2970057T3 (es) | 2011-05-13 | 2024-05-24 | Octapharma Ag | Un procedimiento para aumentar la productividad de células eucarióticas en la producción de FVIII recombinante |
| PT2837680T (pt) | 2011-07-01 | 2020-04-17 | Amgen Inc | Cultura celular de mamífero |
| CN203129697U (zh) | 2013-02-05 | 2013-08-14 | 客贝利(厦门)休闲用品有限公司 | 一种帐篷架杆 |
| US20140315294A1 (en) | 2011-11-24 | 2014-10-23 | Genethon | Scalable lentiviral vector production system compatible with industrial pharmaceutical applications |
| US9574167B2 (en) | 2013-02-15 | 2017-02-21 | Pharyx, Inc. | Methods and apparatus for independent control of product and reactant concentrations |
| AU2014226126B2 (en) | 2013-03-08 | 2019-02-14 | Genzyme Corporation | Continuous purification of therapeutic proteins |
| US9217168B2 (en) | 2013-03-14 | 2015-12-22 | Momenta Pharmaceuticals, Inc. | Methods of cell culture |
| ES2842350T3 (es) | 2013-03-19 | 2021-07-13 | Cmc Biologics As | Un procedimiento para la producción de un producto (por ejemplo, polipéptido) en un proceso de fermentación de cultivo celular continuo |
| JP6851827B2 (ja) * | 2013-08-30 | 2021-03-31 | グラクソスミスクライン バイオロジカルズ ソシエテ アノニム | 細胞培養中でのウイルスの大規模製造 |
| US11390663B2 (en) | 2013-10-11 | 2022-07-19 | Regeneron Pharmaceuticals, Inc. | Metabolically optimized cell culture |
| TWI709569B (zh) | 2014-01-17 | 2020-11-11 | 美商健臻公司 | 無菌層析樹脂及其用於製造方法的用途 |
| TWI709570B (zh) | 2014-01-17 | 2020-11-11 | 美商健臻公司 | 無菌層析法及製法 |
| JP2017509659A (ja) | 2014-04-01 | 2017-04-06 | アドヴァンテック・バイオサイエンス・ファルマスーティカ・リミターダ | 低糖−低グリシンの安定な第ｖｉｉｉ因子製剤 |
| US9855319B2 (en) | 2014-04-01 | 2018-01-02 | Advantech Bioscience Farmacêutica Ltda | Stabilization of factor VIII without calcium as an excipient |
| CN106414719B (zh) * | 2014-06-13 | 2020-02-14 | 杰特有限公司 | 生物反应器中重组von Willebrand因子的改进生产 |
| KR20240173213A (ko) | 2016-01-26 | 2024-12-10 | 베링거 인겔하임 인터내셔날 게엠베하 | 연결된 관류 대 연속-유동식 교반-탱크 반응기 세포 배양 시스템 |
| WO2017180814A1 (en) | 2016-04-15 | 2017-10-19 | Boehringer Ingelheim International Gmbh | Cell retention device and method |
| US20200063086A1 (en) * | 2016-12-21 | 2020-02-27 | Hoffmann-La Roche Inc. | Growth control of eukaryotic cells |
| IL307155B2 (en) | 2018-05-01 | 2025-11-01 | Amgen Inc | Antibodies with modulated glycan profiles |
| WO2019239780A1 (ja) * | 2018-06-15 | 2019-12-19 | 富士フイルム株式会社 | 細胞培養方法、細胞培養装置、及び生産物の製造方法 |
| AU2019332764B2 (en) | 2018-08-31 | 2025-05-29 | Genzyme Corporation | Sterile chromatography resin and use thereof in manufacturing processes |
| CN113355297A (zh) * | 2021-06-23 | 2021-09-07 | 吉林冠界生物技术有限公司 | 一种灌流式培养全悬浮mdck细胞生产重组禽流感病毒的方法 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5019512A (en) | 1989-03-17 | 1991-05-28 | Baxter International Inc. | Spin filter for removing substantially cell-free culture medium from suspension cell culture system |
| US5626734A (en) | 1995-08-18 | 1997-05-06 | University Technologies International, Inc. | Filter for perfusion cultures of animal cells and the like |
| BR0114373A (pt) | 2000-10-02 | 2004-02-17 | Novo Nordisk As | Célula hospedeira eucariótica, métodos para a produção de fvii e de fviia ou de suas variantes, de uma proteìna dependente de vitamina k e de uma célula hospedeira eucariótica produtora de fvii ou de seu análogo, vetor recombinante, fvii e fviia recombinante ou suas variantes, e, proteìna dependente de vitamina k recombinante |
| CA2466881A1 (en) * | 2001-11-28 | 2003-06-05 | Sandoz Gmbh | Cell culture process |
| TWI384069B (zh) | 2004-08-27 | 2013-02-01 | Pfizer Ireland Pharmaceuticals | 多胜肽之製法 |
| ATE544866T1 (de) | 2005-06-17 | 2012-02-15 | Baxter Int | Adamts13-haltige zusammensetzungen mit thrombolytischer wirkung |
| ES2474573T3 (es) | 2006-01-04 | 2014-07-09 | Baxter International Inc | Medio de cultivo celular sin oligop�ptidos |
| SG187397A1 (en) * | 2007-12-27 | 2013-02-28 | Baxter Int | Cell culture processes |
-
2010
- 2010-07-30 US US12/847,921 patent/US8580554B2/en active Active
- 2010-08-02 DK DK10737916.6T patent/DK2459697T3/en active
- 2010-08-02 HR HRP20182165TT patent/HRP20182165T1/hr unknown
- 2010-08-02 IN IN899DEN2012 patent/IN2012DN00899A/en unknown
- 2010-08-02 ES ES10737916T patent/ES2705206T3/es active Active
- 2010-08-02 EP EP10737916.6A patent/EP2459697B1/en active Active
- 2010-08-02 NZ NZ597742A patent/NZ597742A/en unknown
- 2010-08-02 EP EP18199855.0A patent/EP3480292A1/en not_active Withdrawn
- 2010-08-02 KR KR1020177017512A patent/KR101885046B1/ko active Active
- 2010-08-02 CA CA2769354A patent/CA2769354A1/en not_active Abandoned
- 2010-08-02 KR KR1020127004996A patent/KR101752789B1/ko active Active
- 2010-08-02 AU AU2010277490A patent/AU2010277490B2/en active Active
- 2010-08-02 PL PL10737916T patent/PL2459697T3/pl unknown
- 2010-08-02 BR BR112012002137A patent/BR112012002137B8/pt active IP Right Grant
- 2010-08-02 SG SG2012006599A patent/SG178176A1/en unknown
- 2010-08-02 EA EA201270213A patent/EA022219B1/ru not_active IP Right Cessation
- 2010-08-02 MX MX2012001274A patent/MX2012001274A/es active IP Right Grant
- 2010-08-02 PT PT10737916T patent/PT2459697T/pt unknown
- 2010-08-02 SI SI201031835T patent/SI2459697T1/sl unknown
- 2010-08-02 CN CN201080036754.2A patent/CN102549142B/zh active Active
- 2010-08-02 WO PCT/EP2010/061191 patent/WO2011012725A1/en not_active Ceased
- 2010-08-02 JP JP2012522190A patent/JP5808742B2/ja active Active
- 2010-08-02 TR TR2019/00213T patent/TR201900213T4/tr unknown
-
2012
- 2012-01-27 MX MX2014008515A patent/MX353610B/es unknown
- 2012-02-06 CO CO12020130A patent/CO6491119A2/es unknown
-
2013
- 2013-10-10 US US14/050,710 patent/US9650612B2/en active Active
-
2015
- 2015-01-14 JP JP2015004718A patent/JP6177260B2/ja active Active
-
2017
- 2017-04-12 US US15/486,181 patent/US10221400B2/en active Active
-
2019
- 2019-01-18 US US16/252,216 patent/US10745672B2/en active Active
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| BR112012002137B8 (pt) | método para produzir um polipeptídeo e/ou vírus de interesse em uma cultura de célula contínua isenta de soro | |
| Gjoerup et al. | Update on human polyomaviruses and cancer | |
| Chen et al. | The impacts of climate change and human activities on biogeochemical cycles on the Q inghai‐T ibetan P lateau | |
| AU2019268194A1 (en) | Cell culture system | |
| AR132549A2 (es) | Medio de cultivo para células cho libre de suero que comprende ornitina y putrescina, método para cultivar células cho y método in vitro para producir una proteína | |
| AR069956A1 (es) | Procesos para cultivar celulas mamiferas secretoras de proteinas sanguineas heterologas | |
| WO2010089151A8 (en) | Bioreactor for the cultivation of mammalian cells | |
| BR112013025086A2 (pt) | sistema e métodos de biorreator multifuncional para classificação e cultura de células | |
| BR112015022971A2 (pt) | métodos de fabricação para controlar o teor de lisina c-terminal, galactose e ácido siálico em proteínas recombinantes | |
| MY178233A (en) | Tagged chimeric effector molecules and receptors thereof | |
| BR112016027290A2 (pt) | métodos para a produção de ramnolipídeos". | |
| BR112012019023A2 (pt) | método para produzir adenovírus recombinante sorotipo 26 (rad26), e, usoi de um biorreator | |
| BR112012009828A8 (pt) | método para a produção de uma imunoglobulina glicosilada | |
| MX2017008338A (es) | Metodos de cultivo de una celula de mamifero. | |
| EA201170024A1 (ru) | Способы получения высокой плотности жизнеспособных клеток в культуре клеток млекопитающих | |
| BR112015019156A2 (pt) | cultivo autotrófico | |
| BRPI1008762B8 (pt) | métodos para seleção de células de mamífero expressando uma proteína heteróloga, para produzir um produto de interesse e uso de um meio de cultura seletivo | |
| BR112017017303B8 (pt) | Método para obtenção de clones de células de alta produtividade | |
| Polianskaia | The problem of genomic instability of cultivated human stem cells | |
| MX353243B (es) | Metodo para incrementar la secrecion de proteinas recombinantes. | |
| BR112012033050A2 (pt) | método e sistema para cultivo de microalgas em um reator de fluxo de tampão expandido | |
| Valencia Gonzalez et al. | A field study on the improvement of a tropical soil with biotechnological techniques | |
| NZ716434A (en) | Method for selecting a permissive cell line for replicating avian viruses | |
| Painuly et al. | Influence of anisotropy in sediment mechanical properties on CH4 bubble growth topology and migration pattern in muddy aquatic sediments | |
| BR112015030015B8 (pt) | Método e sistema para propagação de etanologênio para uso na produção de um produto de fermentação compreendendo etanol de biomassa |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B25C | Requirement related to requested transfer of rights |
Owner name: BAXTER HEALTHCARE S.A. (CH) , BAXTER INTERNACIONAL |
|
| B25A | Requested transfer of rights approved |
Owner name: BAXALTA GMBH (CH) , BAXALTA INCORPORATED (US) |
|
| B07A | Application suspended after technical examination (opinion) [chapter 7.1 patent gazette] | ||
| B09A | Decision: intention to grant [chapter 9.1 patent gazette] | ||
| B16A | Patent or certificate of addition of invention granted [chapter 16.1 patent gazette] |
Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 02/08/2010, OBSERVADAS AS CONDICOES LEGAIS. |
|
| B16C | Correction of notification of the grant [chapter 16.3 patent gazette] |
Free format text: REF. RPI 2571 DE 14/04/2020 QUANTO AO ENDERECO. |
|
| B25A | Requested transfer of rights approved |
Owner name: TAKEDA PHARMACEUTICAL COMPANY LIMITED (JP) |
|
| B25L | Entry of change of name and/or headquarter and transfer of application, patent and certificate of addition of invention: publication cancelled |
Owner name: BAXALTA INCORPORATED (US) ; BAXALTA GMBH (CH) Free format text: ANULADA A PUBLICACAO CODIGO 25.1 NA RPI NO 2652 DE 03/11/2021 POR TER SIDO INDEVIDA. |
|
| B25A | Requested transfer of rights approved |
Owner name: TAKEDA PHARMACEUTICAL COMPANY LIMITED (JP) |