BR102014027448A8 - cassete de doador de polinucleotídeo, uso de uma planta, de uma parte de planta, ou de uma célula vegetal compreendendo o referido cassete, e método para integração direcionada do mesmo - Google Patents
cassete de doador de polinucleotídeo, uso de uma planta, de uma parte de planta, ou de uma célula vegetal compreendendo o referido cassete, e método para integração direcionada do mesmoInfo
- Publication number
- BR102014027448A8 BR102014027448A8 BR102014027448A BR102014027448A BR102014027448A8 BR 102014027448 A8 BR102014027448 A8 BR 102014027448A8 BR 102014027448 A BR102014027448 A BR 102014027448A BR 102014027448 A BR102014027448 A BR 102014027448A BR 102014027448 A8 BR102014027448 A8 BR 102014027448A8
- Authority
- BR
- Brazil
- Prior art keywords
- plant
- cassette
- targeted integration
- polynucleotide donor
- cell
- Prior art date
Links
- 230000010354 integration Effects 0.000 title abstract 2
- 102000040430 polynucleotide Human genes 0.000 title abstract 2
- 108091033319 polynucleotide Proteins 0.000 title abstract 2
- 239000002157 polynucleotide Substances 0.000 title abstract 2
- 238000010363 gene targeting Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
- C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
- C12N15/8212—Colour markers, e.g. beta-glucoronidase [GUS], green fluorescent protein [GFP], carotenoid
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
- C12N15/8275—Glyphosate
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361899543P | 2013-11-04 | 2013-11-04 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| BR102014027448A2 BR102014027448A2 (pt) | 2015-09-15 |
| BR102014027448A8 true BR102014027448A8 (pt) | 2021-08-24 |
Family
ID=53005271
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR102014027448A BR102014027448A8 (pt) | 2013-11-04 | 2014-11-03 | cassete de doador de polinucleotídeo, uso de uma planta, de uma parte de planta, ou de uma célula vegetal compreendendo o referido cassete, e método para integração direcionada do mesmo |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US10077449B2 (enExample) |
| EP (1) | EP3066192B1 (enExample) |
| JP (1) | JP6560203B2 (enExample) |
| KR (1) | KR102248730B1 (enExample) |
| CN (1) | CN105683357B (enExample) |
| AR (1) | AR098283A1 (enExample) |
| AU (1) | AU2014341928B2 (enExample) |
| BR (1) | BR102014027448A8 (enExample) |
| CA (1) | CA2926534A1 (enExample) |
| IL (1) | IL245313B (enExample) |
| RU (1) | RU2687369C2 (enExample) |
| TW (1) | TWI669395B (enExample) |
| WO (1) | WO2015066637A1 (enExample) |
| ZA (1) | ZA201601929B (enExample) |
Families Citing this family (48)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10030245B2 (en) | 2011-03-23 | 2018-07-24 | E I Du Pont De Nemours And Company | Methods for producing a complex transgenic trait locus |
| EP2734621B1 (en) | 2011-07-22 | 2019-09-04 | President and Fellows of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| US20150044192A1 (en) | 2013-08-09 | 2015-02-12 | President And Fellows Of Harvard College | Methods for identifying a target site of a cas9 nuclease |
| US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
| US9340800B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | Extended DNA-sensing GRNAS |
| US9388430B2 (en) | 2013-09-06 | 2016-07-12 | President And Fellows Of Harvard College | Cas9-recombinase fusion proteins and uses thereof |
| US9526784B2 (en) | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
| CA2930015A1 (en) | 2013-11-07 | 2015-05-14 | Editas Medicine, Inc. | Crispr-related methods and compositions with governing grnas |
| US9840699B2 (en) | 2013-12-12 | 2017-12-12 | President And Fellows Of Harvard College | Methods for nucleic acid editing |
| WO2016022363A2 (en) | 2014-07-30 | 2016-02-11 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
| WO2016040030A1 (en) | 2014-09-12 | 2016-03-17 | E. I. Du Pont De Nemours And Company | Generation of site-specific-integration sites for complex trait loci in corn and soybean, and methods of use |
| CN107250148B (zh) | 2014-12-03 | 2021-04-16 | 安捷伦科技有限公司 | 具有化学修饰的指导rna |
| JP2018503392A (ja) | 2015-01-27 | 2018-02-08 | 中国科学院遺▲伝▼与▲発▼育生物学研究所Institute of Genetics and Developmental Biology, Chinese Academy of Sciences | 遺伝子一過性発現により完全な植物において部位特異的改変を行うための方法 |
| WO2016164356A1 (en) | 2015-04-06 | 2016-10-13 | The Board Of Trustees Of The Leland Stanford Junior University | Chemically modified guide rnas for crispr/cas-mediated gene regulation |
| EP3095870A1 (en) | 2015-05-19 | 2016-11-23 | Kws Saat Se | Methods for the in planta transformation of plants and manufacturing processes and products based and obtainable therefrom |
| CA2994883A1 (en) * | 2015-08-14 | 2017-02-23 | Institute Of Genetics And Developmental Biology, Chinese Academy Of Scnces | Method for obtaining glyphosate-resistant rice by site-directed nucleotide substitution |
| CN108431932A (zh) * | 2015-09-04 | 2018-08-21 | 欧克特沃系统有限责任公司 | 使用封装部件中的系统的改进的系统 |
| IL258821B (en) | 2015-10-23 | 2022-07-01 | Harvard College | Nucleobase editors and their uses |
| US11306308B2 (en) | 2015-11-13 | 2022-04-19 | Massachusetts Institute Of Technology | High-throughput CRISPR-based library screening |
| US10767175B2 (en) | 2016-06-08 | 2020-09-08 | Agilent Technologies, Inc. | High specificity genome editing using chemically modified guide RNAs |
| KR20250103795A (ko) | 2016-08-03 | 2025-07-07 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 아데노신 핵염기 편집제 및 그의 용도 |
| CA3033327A1 (en) | 2016-08-09 | 2018-02-15 | President And Fellows Of Harvard College | Programmable cas9-recombinase fusion proteins and uses thereof |
| WO2018039438A1 (en) | 2016-08-24 | 2018-03-01 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| JP7588390B2 (ja) | 2016-10-14 | 2024-11-22 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 核酸塩基エディターのaav送達 |
| WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
| CN106591293A (zh) * | 2016-12-28 | 2017-04-26 | 贵州省草业研究所 | 基于酶切连接从未知基因组中分离已知序列侧翼序列的方法 |
| CN110662556A (zh) | 2017-03-09 | 2020-01-07 | 哈佛大学的校长及成员们 | 癌症疫苗 |
| WO2018165504A1 (en) | 2017-03-09 | 2018-09-13 | President And Fellows Of Harvard College | Suppression of pain by gene editing |
| JP2020510439A (ja) | 2017-03-10 | 2020-04-09 | プレジデント アンド フェローズ オブ ハーバード カレッジ | シトシンからグアニンへの塩基編集因子 |
| BR112019019655A2 (pt) | 2017-03-23 | 2020-04-22 | Harvard College | editores de nucleobase que compreendem proteínas de ligação a dna programáveis por ácido nucleico |
| WO2018209320A1 (en) | 2017-05-12 | 2018-11-15 | President And Fellows Of Harvard College | Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation |
| JP2020534795A (ja) | 2017-07-28 | 2020-12-03 | プレジデント アンド フェローズ オブ ハーバード カレッジ | ファージによって支援される連続的進化(pace)を用いて塩基編集因子を進化させるための方法および組成物 |
| US11319532B2 (en) | 2017-08-30 | 2022-05-03 | President And Fellows Of Harvard College | High efficiency base editors comprising Gam |
| KR20250107288A (ko) | 2017-10-16 | 2025-07-11 | 더 브로드 인스티튜트, 인코퍼레이티드 | 아데노신 염기 편집제의 용도 |
| CN107545153B (zh) * | 2017-10-25 | 2021-06-11 | 桂林电子科技大学 | 一种基于卷积神经网络的核小体分类预测方法 |
| WO2019118949A1 (en) | 2017-12-15 | 2019-06-20 | The Broad Institute, Inc. | Systems and methods for predicting repair outcomes in genetic engineering |
| WO2019226953A1 (en) | 2018-05-23 | 2019-11-28 | The Broad Institute, Inc. | Base editors and uses thereof |
| BR112021007229A2 (pt) | 2018-10-16 | 2021-08-10 | Blueallele, Llc | métodos para inserção dirigida de dna em genes |
| WO2020092453A1 (en) | 2018-10-29 | 2020-05-07 | The Broad Institute, Inc. | Nucleobase editors comprising geocas9 and uses thereof |
| US12351837B2 (en) | 2019-01-23 | 2025-07-08 | The Broad Institute, Inc. | Supernegatively charged proteins and uses thereof |
| EP3942042A1 (en) | 2019-03-19 | 2022-01-26 | The Broad Institute, Inc. | Methods and compositions for editing nucleotide sequences |
| EP3956349A1 (en) | 2019-04-17 | 2022-02-23 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| US12435330B2 (en) | 2019-10-10 | 2025-10-07 | The Broad Institute, Inc. | Methods and compositions for prime editing RNA |
| WO2021226558A1 (en) | 2020-05-08 | 2021-11-11 | The Broad Institute, Inc. | Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence |
| CN114317588B (zh) * | 2020-09-29 | 2023-06-27 | 中国农业科学院生物技术研究所 | 一种作物高温响应型基因组精准甲基化调控回路 |
| US11884915B2 (en) | 2021-09-10 | 2024-01-30 | Agilent Technologies, Inc. | Guide RNAs with chemical modification for prime editing |
| WO2023049728A1 (en) * | 2021-09-21 | 2023-03-30 | Pairwise Plants Services, Inc. | Color-based and/or visual methods for identifying the presence of a transgene and compositions and constructs relating to the same |
| CN114369677A (zh) * | 2022-01-04 | 2022-04-19 | 江汉大学 | 一种检测小麦转基因成分和转基因品系的引物组合、试剂盒、检测方法及应用 |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1354035B1 (en) * | 2001-01-26 | 2016-08-24 | Commonwealth Scientific and Industrial Research Organisation | Methods and means for producing efficient silencing construct using recombinational cloning |
| AU2003218382B2 (en) * | 2002-03-21 | 2007-12-13 | Sangamo Therapeutics, Inc. | Methods and compositions for using zinc finger endonucleases to enhance homologous recombination |
| US9428756B2 (en) * | 2006-08-11 | 2016-08-30 | Dow Agrosciences Llc | Zinc finger nuclease-mediated homologous recombination |
| EP2160467B1 (en) * | 2007-06-05 | 2015-10-28 | Bayer CropScience NV | Methods and means for exact replacement of target dna in eukaryotic organisms |
| WO2010002984A1 (en) * | 2008-07-01 | 2010-01-07 | Monsanto Technology, Llc | Recombinant dna constructs and methods for modulating expression of a target gene |
| EP2370575B1 (en) * | 2008-12-17 | 2017-11-01 | Dow AgroSciences LLC | Targeted integration into the zp15 locus |
| MX2011009415A (es) * | 2009-03-20 | 2011-09-27 | Basf Plant Science Co Gmbh | Plantas transgenicas resistentes a los nematodos. |
| US9695432B2 (en) * | 2010-01-22 | 2017-07-04 | Dow Agrosciences Llc | Excision of transgenes in genetically modified organisms |
| WO2011091317A2 (en) * | 2010-01-22 | 2011-07-28 | Dow Agrosciences Llc | Engineered landing pads for gene targeting in plants |
| WO2011154158A1 (en) | 2010-06-09 | 2011-12-15 | Bayer Bioscience N.V. | Methods and means to modify a plant genome at a nucleotide sequence commonly used in plant genome engineering |
| US9540623B2 (en) | 2011-07-08 | 2017-01-10 | Cellectis | Method for increasing the efficiency of double-strand-break induced mutagenesis |
| JP6649258B2 (ja) * | 2013-09-04 | 2020-02-19 | ダウ アグロサイエンシィズ エルエルシー | ドナー挿入を判定するための作物における迅速ターゲッティング解析 |
-
2014
- 2014-11-03 CN CN201480060417.5A patent/CN105683357B/zh not_active Expired - Fee Related
- 2014-11-03 CA CA2926534A patent/CA2926534A1/en not_active Abandoned
- 2014-11-03 AR ARP140104120A patent/AR098283A1/es unknown
- 2014-11-03 TW TW103138081A patent/TWI669395B/zh not_active IP Right Cessation
- 2014-11-03 EP EP14858498.0A patent/EP3066192B1/en not_active Not-in-force
- 2014-11-03 BR BR102014027448A patent/BR102014027448A8/pt not_active Application Discontinuation
- 2014-11-03 KR KR1020167014602A patent/KR102248730B1/ko not_active Expired - Fee Related
- 2014-11-03 WO PCT/US2014/063732 patent/WO2015066637A1/en not_active Ceased
- 2014-11-03 US US14/531,710 patent/US10077449B2/en not_active Expired - Fee Related
- 2014-11-03 JP JP2016526812A patent/JP6560203B2/ja not_active Expired - Fee Related
- 2014-11-03 AU AU2014341928A patent/AU2014341928B2/en not_active Ceased
- 2014-11-03 RU RU2016122038A patent/RU2687369C2/ru active
-
2016
- 2016-03-18 ZA ZA2016/01929A patent/ZA201601929B/en unknown
- 2016-04-25 IL IL245313A patent/IL245313B/en active IP Right Grant
Also Published As
| Publication number | Publication date |
|---|---|
| AU2014341928B2 (en) | 2017-11-30 |
| EP3066192A4 (en) | 2017-07-19 |
| EP3066192B1 (en) | 2019-05-01 |
| BR102014027448A2 (pt) | 2015-09-15 |
| CA2926534A1 (en) | 2015-05-07 |
| IL245313A0 (en) | 2016-06-30 |
| RU2016122038A3 (enExample) | 2018-06-21 |
| KR102248730B1 (ko) | 2021-05-07 |
| CN105683357A (zh) | 2016-06-15 |
| KR20160079090A (ko) | 2016-07-05 |
| JP2016534727A (ja) | 2016-11-10 |
| TWI669395B (zh) | 2019-08-21 |
| WO2015066637A1 (en) | 2015-05-07 |
| US20150143588A1 (en) | 2015-05-21 |
| JP6560203B2 (ja) | 2019-08-14 |
| AR098283A1 (es) | 2016-05-26 |
| RU2687369C2 (ru) | 2019-05-13 |
| EP3066192A1 (en) | 2016-09-14 |
| TW201522630A (zh) | 2015-06-16 |
| RU2016122038A (ru) | 2017-12-11 |
| CN105683357B (zh) | 2020-10-30 |
| IL245313B (en) | 2019-05-30 |
| US10077449B2 (en) | 2018-09-18 |
| AU2014341928A1 (en) | 2016-04-07 |
| ZA201601929B (en) | 2019-06-26 |
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