BR112016030407A2 - método para introdução de ácido nucleico em pelo menos uma célula de um embrião de semente de planta, semente, e, planta ou parte da mesma - Google Patents

método para introdução de ácido nucleico em pelo menos uma célula de um embrião de semente de planta, semente, e, planta ou parte da mesma

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Publication number
BR112016030407A2
BR112016030407A2 BR112016030407A BR112016030407A BR112016030407A2 BR 112016030407 A2 BR112016030407 A2 BR 112016030407A2 BR 112016030407 A BR112016030407 A BR 112016030407A BR 112016030407 A BR112016030407 A BR 112016030407A BR 112016030407 A2 BR112016030407 A2 BR 112016030407A2
Authority
BR
Brazil
Prior art keywords
plant
seed
nucleic acid
introducing nucleic
cell
Prior art date
Application number
BR112016030407A
Other languages
English (en)
Inventor
Peleg Dotan
Huet Herve
Smirra Iris
Lapidot Miri
Lempel Omri
Original Assignee
Morflora Israel Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Morflora Israel Ltd filed Critical Morflora Israel Ltd
Publication of BR112016030407A2 publication Critical patent/BR112016030407A2/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203—Virus mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

a presente invenção refere-se a métodos de tratamento de sementes e introdução de partículas de ácido nucleico em sementes intactas. em particular, os métodos são protocolos de tratamento de sementes de não iniciação capazes de dispersar os plasmídeos de dna nu para sementes, sem a uso de um microrganismo ou quaisquer meios adicionais, e que não está limitado a plantar espécies.
BR112016030407A 2013-04-25 2015-04-20 método para introdução de ácido nucleico em pelo menos uma célula de um embrião de semente de planta, semente, e, planta ou parte da mesma BR112016030407A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361816044P 2013-04-25 2013-04-25
US14/260,952 US20150040268A1 (en) 2013-04-25 2014-04-24 Methods and compositions for the delivery of nucleic acids to seeds
PCT/IL2015/050416 WO2015162608A1 (en) 2013-04-25 2015-04-20 Methods and compositions for the delivery of nucleic acids to seeds

Publications (1)

Publication Number Publication Date
BR112016030407A2 true BR112016030407A2 (pt) 2017-11-07

Family

ID=52428979

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112016030407A BR112016030407A2 (pt) 2013-04-25 2015-04-20 método para introdução de ácido nucleico em pelo menos uma célula de um embrião de semente de planta, semente, e, planta ou parte da mesma

Country Status (5)

Country Link
US (1) US20150040268A1 (pt)
CN (1) CN106460006A (pt)
AU (1) AU2015249414A1 (pt)
BR (1) BR112016030407A2 (pt)
WO (1) WO2015162608A1 (pt)

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EP3535416A4 (en) 2016-11-04 2020-05-13 Flagship Pioneering Innovations V. Inc. NEW PLANT CELLS, PLANTS AND SEEDS
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US11518999B1 (en) 2018-11-09 2022-12-06 Inari Agriculture Technology, Inc. Plant transformation
SG11202112092TA (en) 2019-06-25 2021-11-29 Inari Agriculture Technology Inc Improved homology dependent repair genome editing
CA3236416A1 (en) 2021-11-01 2023-05-04 Flagship Pioneering Innovations Vii, Llc Polynucleotides for modifying organisms
AU2023209447A1 (en) 2022-01-20 2024-07-18 Flagship Pioneering Innovations Vii, Llc Polynucleotides for modifying organisms
EP4299739A1 (en) 2022-06-30 2024-01-03 Inari Agriculture Technology, Inc. Compositions, systems, and methods for genome editing
EP4299733A1 (en) 2022-06-30 2024-01-03 Inari Agriculture Technology, Inc. Compositions, systems, and methods for genome editing
WO2024005864A1 (en) 2022-06-30 2024-01-04 Inari Agriculture Technology, Inc. Compositions, systems, and methods for genome editing
WO2024005863A1 (en) 2022-06-30 2024-01-04 Inari Agriculture Technology, Inc. Compositions, systems, and methods for genome editing
CN116769797B (zh) * 2023-08-14 2023-10-24 浙江大学海南研究院 一种茉莉酸甲酯及PpyMYC2基因在萌芽中的应用

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Also Published As

Publication number Publication date
US20150040268A1 (en) 2015-02-05
CN106460006A (zh) 2017-02-22
AU2015249414A1 (en) 2016-12-15
WO2015162608A1 (en) 2015-10-29

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B11A Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing
B11Y Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette]