RU2687369C2 - Универсальная донорная система для направленного воздействия на гены - Google Patents

Универсальная донорная система для направленного воздействия на гены Download PDF

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RU2687369C2
RU2687369C2 RU2016122038A RU2016122038A RU2687369C2 RU 2687369 C2 RU2687369 C2 RU 2687369C2 RU 2016122038 A RU2016122038 A RU 2016122038A RU 2016122038 A RU2016122038 A RU 2016122038A RU 2687369 C2 RU2687369 C2 RU 2687369C2
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sequence
seq
gene
plant
dna
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RU2016122038A3 (enExample
RU2016122038A (ru
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Лакшми САСТРИ-ДЕНТ
Стивен Л. ЭВАНС
Райан С. Блю
Цзэхуэй ЦАО
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ДАУ АГРОСАЙЕНСИЗ ЭлЭлСи
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
    • C12N15/8212—Colour markers, e.g. beta-glucoronidase [GUS], green fluorescent protein [GFP], carotenoid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
RU2016122038A 2013-11-04 2014-11-03 Универсальная донорная система для направленного воздействия на гены RU2687369C2 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361899543P 2013-11-04 2013-11-04
US61/899,543 2013-11-04
PCT/US2014/063732 WO2015066637A1 (en) 2013-11-04 2014-11-03 A universal donor system for gene targeting

Publications (3)

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RU2016122038A RU2016122038A (ru) 2017-12-11
RU2016122038A3 RU2016122038A3 (enExample) 2018-06-21
RU2687369C2 true RU2687369C2 (ru) 2019-05-13

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US (1) US10077449B2 (enExample)
EP (1) EP3066192B1 (enExample)
JP (1) JP6560203B2 (enExample)
KR (1) KR102248730B1 (enExample)
CN (1) CN105683357B (enExample)
AR (1) AR098283A1 (enExample)
AU (1) AU2014341928B2 (enExample)
BR (1) BR102014027448A8 (enExample)
CA (1) CA2926534A1 (enExample)
IL (1) IL245313B (enExample)
RU (1) RU2687369C2 (enExample)
TW (1) TWI669395B (enExample)
WO (1) WO2015066637A1 (enExample)
ZA (1) ZA201601929B (enExample)

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US9737604B2 (en) 2013-09-06 2017-08-22 President And Fellows Of Harvard College Use of cationic lipids to deliver CAS9
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US11053481B2 (en) 2013-12-12 2021-07-06 President And Fellows Of Harvard College Fusions of Cas9 domains and nucleic acid-editing domains
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CN105683357A (zh) 2016-06-15
KR102248730B1 (ko) 2021-05-07
IL245313B (en) 2019-05-30
CA2926534A1 (en) 2015-05-07
RU2016122038A3 (enExample) 2018-06-21
US10077449B2 (en) 2018-09-18
US20150143588A1 (en) 2015-05-21
WO2015066637A1 (en) 2015-05-07
TWI669395B (zh) 2019-08-21
IL245313A0 (en) 2016-06-30
JP2016534727A (ja) 2016-11-10
KR20160079090A (ko) 2016-07-05
AU2014341928A1 (en) 2016-04-07
EP3066192A1 (en) 2016-09-14
AR098283A1 (es) 2016-05-26
TW201522630A (zh) 2015-06-16
EP3066192B1 (en) 2019-05-01
BR102014027448A8 (pt) 2021-08-24
CN105683357B (zh) 2020-10-30
AU2014341928B2 (en) 2017-11-30
BR102014027448A2 (pt) 2015-09-15
RU2016122038A (ru) 2017-12-11
JP6560203B2 (ja) 2019-08-14
ZA201601929B (en) 2019-06-26
EP3066192A4 (en) 2017-07-19

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