BR0105795A - Triagem de alta produção de bibliotecas de dna expressadas em fungos filamentosos - Google Patents

Triagem de alta produção de bibliotecas de dna expressadas em fungos filamentosos

Info

Publication number
BR0105795A
BR0105795A BR0105795-2A BR0105795A BR0105795A BR 0105795 A BR0105795 A BR 0105795A BR 0105795 A BR0105795 A BR 0105795A BR 0105795 A BR0105795 A BR 0105795A
Authority
BR
Brazil
Prior art keywords
fungi
throughput screening
dna libraries
protein
filamentous fungi
Prior art date
Application number
BR0105795-2A
Other languages
English (en)
Inventor
Peter Jan Punt
Cornelia Van Zeijl
Cornelius Van Den Hondel
Original Assignee
Mark Aaron Emalfarb
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=21741283&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=BR0105795(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from PCT/US2000/010199 external-priority patent/WO2001025468A1/en
Application filed by Mark Aaron Emalfarb filed Critical Mark Aaron Emalfarb
Publication of BR0105795A publication Critical patent/BR0105795A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1079—Screening libraries by altering the phenotype or phenotypic trait of the host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors

Abstract

""TRIAGEM DE ALTA PRODUçãO DE BIBLIOTECAS DE DNA EXPRESSADAS EM FUNGOS FILAMENTOSOS". Esta invenção fornece um método para a expressão de bibliotecas de DNA exógenas em fungos filamentosos. Os fungos são capazes de processar genes eucarióticos que contêm introns, e podem também conduzir etapas de processamento pós-translacionais, como a glicosilação e dobragem de proteínas. A invenção fornece o uso de fungos com morfologia alterada, que permite a triagem de alta produção e evolução molecular orientada de proteínas expressadas. Os mesmos fungos transformados podem ser usados (como ilustrado na figura 1) para produzir quantidades maiores de proteína para isolamento, caracterização, e testes aplicativos, e podem ser apropriados para produção industrial da proteína também.
BR0105795-2A 2000-04-13 2001-04-13 Triagem de alta produção de bibliotecas de dna expressadas em fungos filamentosos BR0105795A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
PCT/US2000/010199 WO2001025468A1 (en) 1999-10-06 2000-04-13 High-throughput screening of expressed dna libraries in filamentous fungi
PCT/US2001/012335 WO2001079558A1 (en) 2000-04-13 2001-04-13 High-throughput screening of expressed dna libraries in filamentous fungi

Publications (1)

Publication Number Publication Date
BR0105795A true BR0105795A (pt) 2002-06-18

Family

ID=21741283

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0105795-2A BR0105795A (pt) 2000-04-13 2001-04-13 Triagem de alta produção de bibliotecas de dna expressadas em fungos filamentosos

Country Status (14)

Country Link
EP (1) EP1272669B1 (pt)
JP (1) JP5138855B2 (pt)
CN (2) CN102174551A (pt)
AT (1) ATE433486T1 (pt)
AU (1) AU5354401A (pt)
BR (1) BR0105795A (pt)
CA (1) CA2376552A1 (pt)
DE (1) DE60138947D1 (pt)
DK (1) DK1272669T3 (pt)
EA (1) EA006873B1 (pt)
ES (1) ES2328011T3 (pt)
IL (1) IL146935A0 (pt)
MX (1) MXPA01012905A (pt)
WO (1) WO2001079558A1 (pt)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7883872B2 (en) 1996-10-10 2011-02-08 Dyadic International (Usa), Inc. Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
CA2345356C (en) 1998-10-06 2012-10-02 Mark Aaron Emalfarb Transformation system in the field of filamentous fungal hosts
EP2505651A3 (en) 2006-12-10 2013-01-09 Dyadic International, Inc. Isolated fungus with reduced protease activity
US8551751B2 (en) 2007-09-07 2013-10-08 Dyadic International, Inc. BX11 enzymes having xylosidase activity
EP2408910B1 (en) 2009-03-16 2018-04-25 Danisco US Inc. Chrysosporium lucknowense protein production system
DE102009055015B4 (de) * 2009-12-18 2013-12-24 Technische Universität Dresden Hefe-Sekretionssystem auf Basis einer Signalsequenz aus der Hydrophobin 1-Gensequenz von Trichoderma reesei
DK2673290T3 (en) * 2011-04-22 2016-11-14 Danisco Us Inc Filamentous fungi with an altered VISKOSITETSFÆNOTYPE
EP2800809B1 (en) * 2012-01-05 2018-03-07 Glykos Finland Oy Protease deficient filamentous fungal cells and methods of use thereof
DK3019602T3 (en) 2013-07-10 2018-11-12 Glykos Finland Oy MULTIPLE PROTEASE-DEFECTED FILAMENTARY FUNGAL CELLS AND PROCEDURES FOR USE THEREOF
CN105940103B (zh) * 2014-01-31 2021-06-15 株式会社Api 哌可酸4位羟化酶和利用该酶的4-羟基氨基酸的制造法
CA2954974A1 (en) 2014-07-21 2016-01-28 Glykos Finland Oy Production of glycoproteins with mammalian-like n-glycans in filamentous fungi
EP3755710A1 (en) * 2018-04-24 2020-12-30 Danisco US Inc. Filamentous fungal strains comprising reduced viscosity phenotypes
CN108611339B (zh) * 2018-05-11 2021-05-25 中国农业科学院北京畜牧兽医研究所 葡萄糖淀粉酶TlGa15及其基因和应用
CN109136252B (zh) * 2018-08-23 2021-03-26 中国农业科学院北京畜牧兽医研究所 用于改变里氏木霉菌丝形态的重组表达载体及其应用
CN112574899A (zh) * 2020-12-30 2021-03-30 南通大学 一种缺失arp1基因的白念珠菌减毒菌株及其用途

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4816405A (en) * 1984-10-24 1989-03-28 The Regents Of The University Of California Vectors for transformation by ascomycetes
US5364770A (en) * 1985-08-29 1994-11-15 Genencor International Inc. Heterologous polypeptides expressed in aspergillus
US5536661A (en) * 1987-03-10 1996-07-16 Novo Nordisk A/S Process for the production of protein products in aspergillus
MX9206979A (es) * 1991-12-04 1993-07-01 Novo Nordisk As Metodo de clonacion de proteinas en levaduras
US5602004A (en) * 1994-07-20 1997-02-11 Novo Nordisk Biotech, Inc. Thermophilic fungal expression system
DK0877801T3 (da) * 1996-01-19 2005-08-29 Novozymes Biotech Inc Morfologiske mutanter af trådsvampe
CN1198924C (zh) * 1997-12-22 2005-04-27 Dsm公司 在丝状真菌中表达克隆
AU6188599A (en) * 1998-10-26 2000-05-15 Novozymes A/S Constructing and screening a dna library of interest in filamentous fungal cells

Also Published As

Publication number Publication date
EP1272669B1 (en) 2009-06-10
EA200200035A1 (ru) 2002-12-26
ES2328011T3 (es) 2009-11-06
DE60138947D1 (de) 2009-07-23
JP5138855B2 (ja) 2013-02-06
IL146935A0 (en) 2002-08-14
DK1272669T3 (da) 2009-07-13
CN102174551A (zh) 2011-09-07
JP2003530843A (ja) 2003-10-21
MXPA01012905A (es) 2002-11-22
EP1272669A4 (en) 2004-03-17
CA2376552A1 (en) 2001-10-25
WO2001079558A8 (en) 2003-02-27
AU5354401A (en) 2001-10-30
ATE433486T1 (de) 2009-06-15
EA006873B1 (ru) 2006-04-28
EP1272669A1 (en) 2003-01-08
WO2001079558A1 (en) 2001-10-25
CN1380905A (zh) 2002-11-20

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 9A E 10A ANUIDADE(S).

B25A Requested transfer of rights approved

Owner name: DYADIC INTERNATIONAL (USA), INC. (US)

Free format text: TRANSFERIDO DE: MARK AARON EMALFARB

B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE 9A. E 10A. ANUIDADE(S).

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 2108 DE 31/05/2011.