EA200200035A1 - Высокопроизводительный скрининг экспрессионных библиотек днк в гифомицетах - Google Patents

Высокопроизводительный скрининг экспрессионных библиотек днк в гифомицетах

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Publication number
EA200200035A1
EA200200035A1 EA200200035A EA200200035A EA200200035A1 EA 200200035 A1 EA200200035 A1 EA 200200035A1 EA 200200035 A EA200200035 A EA 200200035A EA 200200035 A EA200200035 A EA 200200035A EA 200200035 A1 EA200200035 A1 EA 200200035A1
Authority
EA
Eurasian Patent Office
Prior art keywords
fungi
dna libraries
protein
gifomycetes
high performance
Prior art date
Application number
EA200200035A
Other languages
English (en)
Other versions
EA006873B1 (ru
Inventor
Петер Ян Пунт
Корнелия Ван Зейл
Корнелиус Ван Ден Хондел
Original Assignee
Эмалфарб, Марк Аарон
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=21741283&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=EA200200035(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from PCT/US2000/010199 external-priority patent/WO2001025468A1/en
Application filed by Эмалфарб, Марк Аарон filed Critical Эмалфарб, Марк Аарон
Publication of EA200200035A1 publication Critical patent/EA200200035A1/ru
Publication of EA006873B1 publication Critical patent/EA006873B1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1079—Screening libraries by altering the phenotype or phenotypic trait of the host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

В изобретении предлагается способ экспрессии экзогенных библиотек ДНК в гифомицетах. Грибы способны осуществлять процессинг содержащих интроны генов эукариот, а также могут осуществлять стадии посттрансляционного процессинга, такие как гликозилирование и укладка белка. В изобретении предлагается применение грибов с измененной морфологией, обеспечивающее высокопроизводительный скрининг и направленную молекулярную перестройку экспрессируемых белков. Те же самые трансформированные грибы могут быть применены (как показано на чертеже) для получения больших количеств белка для его выделения, характеристики и проверки на применимость, а также могут быть пригодны для промышленного получения белка.Международная заявка была опубликована вместе с отчетом о международном поиске.
EA200200035A 2000-04-13 2001-04-13 Трансформированные грибы-гифомицеты, способ их получения и способы экспрессии и получения белков при их использовании EA006873B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
PCT/US2000/010199 WO2001025468A1 (en) 1999-10-06 2000-04-13 High-throughput screening of expressed dna libraries in filamentous fungi
PCT/US2001/012335 WO2001079558A1 (en) 2000-04-13 2001-04-13 High-throughput screening of expressed dna libraries in filamentous fungi

Publications (2)

Publication Number Publication Date
EA200200035A1 true EA200200035A1 (ru) 2002-12-26
EA006873B1 EA006873B1 (ru) 2006-04-28

Family

ID=21741283

Family Applications (1)

Application Number Title Priority Date Filing Date
EA200200035A EA006873B1 (ru) 2000-04-13 2001-04-13 Трансформированные грибы-гифомицеты, способ их получения и способы экспрессии и получения белков при их использовании

Country Status (14)

Country Link
EP (1) EP1272669B1 (ru)
JP (1) JP5138855B2 (ru)
CN (2) CN1380905A (ru)
AT (1) ATE433486T1 (ru)
AU (1) AU5354401A (ru)
BR (1) BR0105795A (ru)
CA (1) CA2376552A1 (ru)
DE (1) DE60138947D1 (ru)
DK (1) DK1272669T3 (ru)
EA (1) EA006873B1 (ru)
ES (1) ES2328011T3 (ru)
IL (1) IL146935A0 (ru)
MX (1) MXPA01012905A (ru)
WO (1) WO2001079558A1 (ru)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7883872B2 (en) 1996-10-10 2011-02-08 Dyadic International (Usa), Inc. Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
EP1117808B1 (en) 1998-10-06 2004-12-29 Mark Aaron Emalfarb Transformation system in the field of filamentous fungal hosts: in chrysosporium
EP2505651A3 (en) 2006-12-10 2013-01-09 Dyadic International, Inc. Isolated fungus with reduced protease activity
WO2009033071A2 (en) 2007-09-07 2009-03-12 Dyadic International, Inc. Novel fungal enzymes
BRPI1009488B1 (pt) 2009-03-16 2020-09-15 Danisco Us Inc Cepa de fungo hospedeiro e métodos para produção homóloga e/ou heteróloga de uma proteína pura com uma pureza superior a 75%, para produção de misturas artificiais de proteínas e para triagem simplificada de cepas que expressam 5 funcionalmente uma enzima desejada
DE102009055015B4 (de) * 2009-12-18 2013-12-24 Technische Universität Dresden Hefe-Sekretionssystem auf Basis einer Signalsequenz aus der Hydrophobin 1-Gensequenz von Trichoderma reesei
US9587242B2 (en) * 2011-04-22 2017-03-07 Danisco Us Inc. Filamentous fungi having an altered viscosity phenotype
CN104245919B (zh) * 2012-01-05 2017-07-14 诺华股份有限公司 蛋白酶缺陷丝状真菌细胞及其使用方法
CA2916905A1 (en) 2013-07-10 2015-01-15 Novartis Ag Multiple proteases deficient filamentous fungal cells and methods of use thereof
EP3101123B1 (en) * 2014-01-31 2019-12-11 API Corporation Pipecolinic acid position-4 hydroxylase and method for producing 4-hydroxyamino acid using same
EP3172333B1 (en) 2014-07-21 2020-05-13 Glykos Finland Oy Production of glycoproteins with mammalian-like n-glycans in filamentous fungi
WO2019209576A1 (en) * 2018-04-24 2019-10-31 Danisco Us Inc Filamentous fungal strains comprising reduced viscosity phenotypes
CN108611339B (zh) * 2018-05-11 2021-05-25 中国农业科学院北京畜牧兽医研究所 葡萄糖淀粉酶TlGa15及其基因和应用
CN109136252B (zh) * 2018-08-23 2021-03-26 中国农业科学院北京畜牧兽医研究所 用于改变里氏木霉菌丝形态的重组表达载体及其应用
CN112574899A (zh) * 2020-12-30 2021-03-30 南通大学 一种缺失arp1基因的白念珠菌减毒菌株及其用途

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4816405A (en) * 1984-10-24 1989-03-28 The Regents Of The University Of California Vectors for transformation by ascomycetes
US5364770A (en) * 1985-08-29 1994-11-15 Genencor International Inc. Heterologous polypeptides expressed in aspergillus
US5536661A (en) * 1987-03-10 1996-07-16 Novo Nordisk A/S Process for the production of protein products in aspergillus
MX9206979A (es) * 1991-12-04 1993-07-01 Novo Nordisk As Metodo de clonacion de proteinas en levaduras
US5602004A (en) * 1994-07-20 1997-02-11 Novo Nordisk Biotech, Inc. Thermophilic fungal expression system
CN1231588C (zh) * 1996-01-19 2005-12-14 诺沃奇梅兹生物技术有限公司 丝状真菌的形态突变体
AU736111C (en) * 1997-12-22 2004-02-19 Dsm Ip Assets B.V. Expression cloning in filamentous fungi
AU6188599A (en) * 1998-10-26 2000-05-15 Novozymes A/S Constructing and screening a dna library of interest in filamentous fungal cells

Also Published As

Publication number Publication date
BR0105795A (pt) 2002-06-18
WO2001079558A8 (en) 2003-02-27
DK1272669T3 (da) 2009-07-13
AU5354401A (en) 2001-10-30
EP1272669B1 (en) 2009-06-10
IL146935A0 (en) 2002-08-14
ATE433486T1 (de) 2009-06-15
ES2328011T3 (es) 2009-11-06
EA006873B1 (ru) 2006-04-28
JP2003530843A (ja) 2003-10-21
JP5138855B2 (ja) 2013-02-06
CN102174551A (zh) 2011-09-07
EP1272669A4 (en) 2004-03-17
DE60138947D1 (de) 2009-07-23
MXPA01012905A (es) 2002-11-22
CA2376552A1 (en) 2001-10-25
WO2001079558A1 (en) 2001-10-25
EP1272669A1 (en) 2003-01-08
CN1380905A (zh) 2002-11-20

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Legal Events

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MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): AM AZ BY KZ KG MD TJ TM

PC4A Registration of transfer of a eurasian patent by assignment