AU742270B2 - Direct expression of peptides into culture media - Google Patents

Direct expression of peptides into culture media Download PDF

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Publication number
AU742270B2
AU742270B2 AU71279/98A AU7127998A AU742270B2 AU 742270 B2 AU742270 B2 AU 742270B2 AU 71279/98 A AU71279/98 A AU 71279/98A AU 7127998 A AU7127998 A AU 7127998A AU 742270 B2 AU742270 B2 AU 742270B2
Authority
AU
Australia
Prior art keywords
peptide
peptide product
recovering
vector
product
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU71279/98A
Other languages
English (en)
Other versions
AU7127998A (en
Inventor
Angelo P. Consalvo
Christopher P. Meenan
Nozar M. Mehta
Martha V. L. Ray
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Enteris Biopharma Inc
Original Assignee
Unigene Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Unigene Laboratories Inc filed Critical Unigene Laboratories Inc
Publication of AU7127998A publication Critical patent/AU7127998A/en
Application granted granted Critical
Publication of AU742270B2 publication Critical patent/AU742270B2/en
Priority to AU15679/02A priority Critical patent/AU781978B2/en
Assigned to ENTERIS BIOPHARMA, INC. reassignment ENTERIS BIOPHARMA, INC. Alteration of Name(s) in Register under S187 Assignors: UNIGENE LABORATORIES, INC.
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/22—Hormones
    • A61K38/23—Calcitonins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/461—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from fish
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/585—Calcitonins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/635—Parathyroid hormone, i.e. parathormone; Parathyroid hormone-related peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/71—Expression systems using regulatory sequences derived from the trp-operon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/72—Expression systems using regulatory sequences derived from the lac-operon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Endocrinology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Plant Pathology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Immunology (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
AU71279/98A 1997-04-16 1998-04-15 Direct expression of peptides into culture media Ceased AU742270B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU15679/02A AU781978B2 (en) 1997-04-16 2002-02-19 Direct expression of peptides into culture media

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US4370097P 1997-04-16 1997-04-16
US60/043700 1997-04-16
PCT/US1998/007723 WO1998046722A1 (en) 1997-04-16 1998-04-15 Direct expression of peptides into culture media

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU15679/02A Division AU781978B2 (en) 1997-04-16 2002-02-19 Direct expression of peptides into culture media

Publications (2)

Publication Number Publication Date
AU7127998A AU7127998A (en) 1998-11-11
AU742270B2 true AU742270B2 (en) 2001-12-20

Family

ID=21928441

Family Applications (1)

Application Number Title Priority Date Filing Date
AU71279/98A Ceased AU742270B2 (en) 1997-04-16 1998-04-15 Direct expression of peptides into culture media

Country Status (14)

Country Link
US (5) US6103495A (cg-RX-API-DMAC7.html)
EP (2) EP2088188B1 (cg-RX-API-DMAC7.html)
JP (2) JP4907751B2 (cg-RX-API-DMAC7.html)
AT (2) ATE522598T1 (cg-RX-API-DMAC7.html)
AU (1) AU742270B2 (cg-RX-API-DMAC7.html)
CA (1) CA2285658C (cg-RX-API-DMAC7.html)
DE (1) DE69841939D1 (cg-RX-API-DMAC7.html)
DK (2) DK1005526T3 (cg-RX-API-DMAC7.html)
ES (1) ES2375330T3 (cg-RX-API-DMAC7.html)
HU (1) HUP0001272A3 (cg-RX-API-DMAC7.html)
LT (1) LT4701B (cg-RX-API-DMAC7.html)
NO (1) NO327325B1 (cg-RX-API-DMAC7.html)
RU (1) RU2218407C2 (cg-RX-API-DMAC7.html)
WO (1) WO1998046722A1 (cg-RX-API-DMAC7.html)

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US7166575B2 (en) * 2002-12-17 2007-01-23 Nastech Pharmaceutical Company Inc. Compositions and methods for enhanced mucosal delivery of peptide YY and methods for treating and preventing obesity
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US8088734B2 (en) * 2003-01-21 2012-01-03 Unigene Laboratories Inc. Oral delivery of peptides
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US8227241B2 (en) * 2004-03-12 2012-07-24 Unigene Laboratories, Inc. Bacterial host cell for the direct expression of peptides
US7531518B2 (en) * 2004-05-14 2009-05-12 Unigene Laboratories Inc. Method for fostering bone formation and preservation
US7648965B2 (en) * 2004-05-14 2010-01-19 Unigene Laboratories Inc. Method for fostering bone formation and preservation
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WO2006132925A2 (en) * 2005-06-01 2006-12-14 University Of Pittsburgh Of The Commonwealth System Of Higher Education Method of amidated peptide biosynthesis and delivery in vivo: endomorphin-2 for pain therapy
WO2006130806A2 (en) * 2005-06-01 2006-12-07 University Of Pittsburgh Of The Commonwealth System Of Higher Education Assay for agonists and antagonists of ion channels and for regulators of genetic expression
EP1907561B1 (en) 2005-06-24 2013-08-28 Unigene Laboratories, Inc. Cell lines for expressing enzyme useful in the preparation of amidated products
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RU2370280C2 (ru) * 2007-10-08 2009-10-20 Институт физико-химической биологии им. А.Н.Белозерского СПОСОБ СУПЕРПРОДУКЦИИ В РАСТЕНИИ АНТИТЕЛ ПРОТИВ ОНКОГЕНА HER2/neu
US9422559B2 (en) 2010-06-02 2016-08-23 Shi-Lung Lin Production and utilization of a novel anti-cancer drug in therapy
US9399773B2 (en) 2012-08-10 2016-07-26 Shi-Lung Lin Production and extraction of MicroRNA precursor as drug for cancer therapy
ES2622877T3 (es) 2009-01-22 2017-07-07 Keybioscience Ag Tratamiento para la obesidad
BR112013032088B1 (pt) 2011-06-15 2020-11-24 Resother Pharma Aps produtos farmacêuticos anti-inflamatórios
EP2742127B1 (en) 2011-08-12 2019-10-09 Mello Biotechnology, Inc. Inducable expression from the eukaryotic pol-2 promoter in prokaryotes
SI3095484T1 (en) 2011-11-02 2018-08-31 Keybioscience Ag Calcitonin mimetics for the treatment of diseases and disorders
CA2854175A1 (en) 2011-11-02 2013-05-10 Keybioscience Ag Peptide analogs for treating diseases and disorders
US10414808B2 (en) 2012-05-18 2019-09-17 Janssen Biotech, Inc. Huwentoxin-IV variants and methods of use
US9102751B2 (en) * 2012-05-18 2015-08-11 Janssen Biotech, Inc. Huwentoxin-IV variants and methods of use
US10196662B2 (en) 2012-08-10 2019-02-05 Mello Biotechnology, Inc. Composition for producing microRNA precursors as drugs for enhancing wound healing and production method of the microRNA precursors
US9457086B2 (en) 2013-03-05 2016-10-04 Enteris Biopharma, Inc. Pharmaceuticals for oral delivery
BR112016007062A2 (pt) 2013-10-03 2017-09-12 Janssen Biotech Inc variantes de protoxina-ii e métodos de uso
BR112016010643A2 (pt) 2013-11-14 2017-12-05 Keybioscience Ag peptídeo
CN103709236B (zh) * 2013-12-23 2015-04-15 江南大学 一种可提高分泌效率的信号肽及其应用
CA2973099C (en) 2015-01-12 2021-09-14 Enteris Biopharma, Inc. Solid oral dosage forms
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Also Published As

Publication number Publication date
LT4701B (lt) 2000-09-25
DK1005526T3 (da) 2011-02-07
WO1998046722A1 (en) 1998-10-22
ATE522598T1 (de) 2011-09-15
US20040191865A1 (en) 2004-09-30
JP2012024108A (ja) 2012-02-09
WO1998046722A9 (en) 1999-11-18
NO995014D0 (no) 1999-10-14
EP1005526A4 (en) 2005-02-09
US6103495A (en) 2000-08-15
ATE484572T1 (de) 2010-10-15
JP2002501379A (ja) 2002-01-15
US6210925B1 (en) 2001-04-03
US6627438B2 (en) 2003-09-30
EP1005526A1 (en) 2000-06-07
JP4907751B2 (ja) 2012-04-04
HUP0001272A1 (hu) 2000-08-28
DE69841939D1 (de) 2010-11-25
RU2218407C2 (ru) 2003-12-10
LT99133A (en) 2000-04-25
EP2088188B1 (en) 2011-08-31
HUP0001272A3 (en) 2012-12-28
US6737250B2 (en) 2004-05-18
AU7127998A (en) 1998-11-11
EP1005526B1 (en) 2010-10-13
CA2285658C (en) 2011-10-04
US7968311B2 (en) 2011-06-28
US20020045211A1 (en) 2002-04-18
US20010055789A1 (en) 2001-12-27
NO327325B1 (no) 2009-06-08
DK2088188T3 (da) 2012-01-02
NO995014L (no) 1999-12-13
EP2088188A1 (en) 2009-08-12
JP5634971B2 (ja) 2014-12-03
CA2285658A1 (en) 1998-10-22
ES2375330T3 (es) 2012-02-28

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