HUP0001272A1 - Peptidek expressziója közvetlenül a tápközegbe - Google Patents

Peptidek expressziója közvetlenül a tápközegbe

Info

Publication number
HUP0001272A1
HUP0001272A1 HU0001272A HUP0001272A HUP0001272A1 HU P0001272 A1 HUP0001272 A1 HU P0001272A1 HU 0001272 A HU0001272 A HU 0001272A HU P0001272 A HUP0001272 A HU P0001272A HU P0001272 A1 HUP0001272 A1 HU P0001272A1
Authority
HU
Hungary
Prior art keywords
peptide
subject
resistant
peptides
heat
Prior art date
Application number
HU0001272A
Other languages
English (en)
Inventor
Angelo P. Consalvo
Christopher Meenan
Nozar M. Mehta
Martha V. L. Ray
Original Assignee
Unigene Laboratories Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Unigene Laboratories Inc. filed Critical Unigene Laboratories Inc.
Publication of HUP0001272A1 publication Critical patent/HUP0001272A1/hu
Publication of HUP0001272A3 publication Critical patent/HUP0001272A3/hu

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/22—Hormones
    • A61K38/23—Calcitonins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/461—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from fish
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/585—Calcitonins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/635—Parathyroid hormone (parathormone); Parathyroid hormone-related peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/71—Expression systems using regulatory sequences derived from the trp-operon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/72—Expression systems using regulatory sequences derived from the lac-operon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Abstract

A találmány tárgyát peptidtermék expressziója képezi közvetlenül apeptidterméket expresszáló, géntechnőlógiai útőn előállítőtt gazdasejttápközegébe. Közelebbről, a találmány tárgyát expressziós vektőrőkképezik, a expressziós vektőrőkkal transzfőrmált vagy transzfektáltgazdasejtek, valamint fermentációs eljárásők, amelyek alkalmasak őlyanpeptidtermékek előállítására, melyek nagy mennyiségben exkretálódnak agazdasejtből a tápközegbe. A találmány egy megvalósítási módjaszerint, C-terminálisan glicint hőrdőzó peptidterméketexpresszáltatnak közvetlenül a tápközegbe, amely peptidet későbbglicin helyett aminőcsőpőrtőt hőrdőzó amidált peptiddé alakítanak. Atalálmány szerinti megőldás alkalmas különböző pőlipeptidekbiőtechnőlógiai útőn történő ipari előállítására. ŕ
HU0001272A 1997-04-16 1998-04-15 Direct expression of peptides into culture media HUP0001272A3 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US4370097P 1997-04-16 1997-04-16

Publications (2)

Publication Number Publication Date
HUP0001272A1 true HUP0001272A1 (hu) 2000-08-28
HUP0001272A3 HUP0001272A3 (en) 2012-12-28

Family

ID=21928441

Family Applications (1)

Application Number Title Priority Date Filing Date
HU0001272A HUP0001272A3 (en) 1997-04-16 1998-04-15 Direct expression of peptides into culture media

Country Status (14)

Country Link
US (5) US6103495A (hu)
EP (2) EP1005526B1 (hu)
JP (2) JP4907751B2 (hu)
AT (2) ATE522598T1 (hu)
AU (1) AU742270B2 (hu)
CA (1) CA2285658C (hu)
DE (1) DE69841939D1 (hu)
DK (2) DK2088188T3 (hu)
ES (1) ES2375330T3 (hu)
HU (1) HUP0001272A3 (hu)
LT (1) LT4701B (hu)
NO (1) NO327325B1 (hu)
RU (1) RU2218407C2 (hu)
WO (1) WO1998046722A1 (hu)

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CA2854175A1 (en) 2011-11-02 2013-05-10 Keybioscience Ag Peptide analogs for treating diseases and disorders
US10414808B2 (en) 2012-05-18 2019-09-17 Janssen Biotech, Inc. Huwentoxin-IV variants and methods of use
US9102751B2 (en) * 2012-05-18 2015-08-11 Janssen Biotech, Inc. Huwentoxin-IV variants and methods of use
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EP2964193B1 (en) 2013-03-05 2019-12-11 Enteris BioPharma, Inc. Pharmaceuticals for oral delivery
CN105793277B (zh) 2013-10-03 2020-09-15 詹森生物科技公司 原毒素-ii变体及其使用方法
PL3321278T3 (pl) 2013-11-14 2019-06-28 Keybioscience Ag Mimetyki kalcytoniny do leczenia chorób i zaburzeń
CN103709236B (zh) * 2013-12-23 2015-04-15 江南大学 一种可提高分泌效率的信号肽及其应用
KR102294577B1 (ko) 2015-01-12 2021-08-26 엔터리스 바이오파마, 인크. 고체 경구 제형
MA41642A (fr) 2015-03-03 2018-01-09 Janssen Biotech Inc Variants de protoxine ii et méthodes d'utilisation
JP6985151B2 (ja) 2015-04-02 2021-12-22 ヤンセン バイオテツク，インコーポレーテツド プロトキシン−ｉｉ変異体及びその使用方法
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Also Published As

Publication number Publication date
US6210925B1 (en) 2001-04-03
WO1998046722A1 (en) 1998-10-22
AU742270B2 (en) 2001-12-20
NO995014L (no) 1999-12-13
US20010055789A1 (en) 2001-12-27
RU2218407C2 (ru) 2003-12-10
DE69841939D1 (de) 2010-11-25
DK1005526T3 (da) 2011-02-07
US20040191865A1 (en) 2004-09-30
NO995014D0 (no) 1999-10-14
US6627438B2 (en) 2003-09-30
EP2088188B1 (en) 2011-08-31
US6103495A (en) 2000-08-15
JP5634971B2 (ja) 2014-12-03
LT99133A (en) 2000-04-25
US6737250B2 (en) 2004-05-18
NO327325B1 (no) 2009-06-08
WO1998046722A9 (en) 1999-11-18
JP4907751B2 (ja) 2012-04-04
US7968311B2 (en) 2011-06-28
EP1005526B1 (en) 2010-10-13
HUP0001272A3 (en) 2012-12-28
LT4701B (lt) 2000-09-25
ATE484572T1 (de) 2010-10-15
JP2012024108A (ja) 2012-02-09
EP1005526A4 (en) 2005-02-09
ATE522598T1 (de) 2011-09-15
CA2285658C (en) 2011-10-04
CA2285658A1 (en) 1998-10-22
ES2375330T3 (es) 2012-02-28
EP1005526A1 (en) 2000-06-07
EP2088188A1 (en) 2009-08-12
US20020045211A1 (en) 2002-04-18
AU7127998A (en) 1998-11-11
DK2088188T3 (da) 2012-01-02
JP2002501379A (ja) 2002-01-15

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