AU2020382121A1 - Biochemical reaction methods and reagents comprising intrinsically disordered regions - Google Patents

Biochemical reaction methods and reagents comprising intrinsically disordered regions Download PDF

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Publication number
AU2020382121A1
AU2020382121A1 AU2020382121A AU2020382121A AU2020382121A1 AU 2020382121 A1 AU2020382121 A1 AU 2020382121A1 AU 2020382121 A AU2020382121 A AU 2020382121A AU 2020382121 A AU2020382121 A AU 2020382121A AU 2020382121 A1 AU2020382121 A1 AU 2020382121A1
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AU
Australia
Prior art keywords
idr
polypeptide
macromolecule
reaction
amino acid
Prior art date
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Pending
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AU2020382121A
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English (en)
Other versions
AU2020382121A8 (en
Inventor
Niall Armes
Matthew Forrest
Sidong LIU
Lauren PARKER
Mathew PARKER
Hannah WILLIAMS
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biocrucible Ltd
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Biocrucible Ltd
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Filing date
Publication date
Application filed by Biocrucible Ltd filed Critical Biocrucible Ltd
Publication of AU2020382121A1 publication Critical patent/AU2020382121A1/en
Publication of AU2020382121A8 publication Critical patent/AU2020382121A8/en
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/507—Recombinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2522/00—Reaction characterised by the use of non-enzymatic proteins
    • C12Q2522/10—Nucleic acid binding proteins
    • C12Q2522/101—Single or double stranded nucleic acid binding proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/125—Specific component of sample, medium or buffer

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU2020382121A 2019-11-11 2020-11-11 Biochemical reaction methods and reagents comprising intrinsically disordered regions Pending AU2020382121A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB1916379.9A GB201916379D0 (en) 2019-11-11 2019-11-11 Biochemical reaction methods and reagents
GB1916379.9 2019-11-11
PCT/GB2020/052866 WO2021094746A1 (en) 2019-11-11 2020-11-11 Biochemical reaction methods and reagents comprising intrinsically disordered regions

Publications (2)

Publication Number Publication Date
AU2020382121A1 true AU2020382121A1 (en) 2022-06-16
AU2020382121A8 AU2020382121A8 (en) 2022-07-28

Family

ID=69062276

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2020382121A Pending AU2020382121A1 (en) 2019-11-11 2020-11-11 Biochemical reaction methods and reagents comprising intrinsically disordered regions

Country Status (13)

Country Link
US (1) US11807899B2 (enExample)
EP (1) EP4058599A1 (enExample)
JP (2) JP7840266B2 (enExample)
KR (1) KR20220113948A (enExample)
CN (1) CN114945682A (enExample)
AU (1) AU2020382121A1 (enExample)
BR (1) BR112022009072A2 (enExample)
CA (1) CA3157435A1 (enExample)
GB (1) GB201916379D0 (enExample)
IL (1) IL292885A (enExample)
MX (1) MX2022005677A (enExample)
WO (1) WO2021094746A1 (enExample)
ZA (1) ZA202205093B (enExample)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108998406B (zh) * 2018-08-03 2022-05-10 福州大学 一种人类原代培养细胞基因组编辑、定点基因敲入方法
CN113355389B (zh) * 2020-03-05 2022-11-15 广西扬翔股份有限公司 利用CRISPR/Cas12a系统靶向富集核酸目标区域的方法及其应用
US20240110221A1 (en) * 2022-09-30 2024-04-04 Illumina, Inc. Methods of modulating clustering kinetics
CN116251621B (zh) * 2022-12-13 2024-08-23 中国科学院基础医学与肿瘤研究所(筹) 一种提高氯化血红素的过氧化物酶活性的方法
WO2024178628A1 (en) * 2023-02-28 2024-09-06 Sun Yat-Sen University Epigenetic editor assisted by phase separation proteins
JP2024179837A (ja) * 2023-06-16 2024-12-26 国立大学法人 東京大学 酵素の検出方法及び酵素の検出キット
KR20250022285A (ko) 2023-08-07 2025-02-17 포항공과대학교 산학협력단 액체-액체 상분리를 통한 scotin 프롤린-풍부 도메인 생체분자 응축물 및 그의 응용
GB2643378A (en) * 2024-07-15 2026-02-18 Biocrucible Ltd Reagents and methods

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
US7270981B2 (en) 2002-02-21 2007-09-18 Asm Scientific, Inc. Recombinase polymerase amplification
WO2005103670A1 (ja) 2004-04-21 2005-11-03 Toray Industries, Inc. ラボオンチップ用基板
DE102005052752A1 (de) 2005-11-04 2007-05-10 Clondiag Chip Technologies Gmbh Vorrichtung und Verfahren zum Nachweis von molekularen Wechselwirkungen
WO2007096702A2 (en) 2005-07-25 2007-08-30 Asm Scientific, Inc. Methods for multiplexing recombinase polymerase amplification
US20070059713A1 (en) * 2005-09-09 2007-03-15 Lee Jun E SSB-DNA polymerase fusion proteins
US8071308B2 (en) * 2006-05-04 2011-12-06 Alere San Diego, Inc. Recombinase polymerase amplification
WO2008108927A2 (en) * 2007-03-06 2008-09-12 Regenerx Biopharmaceuticals, Inc. Methods and compositions for stabilizing polypeptides
CN101743063B (zh) 2007-05-03 2012-12-12 科隆迪亚戈有限公司 分析方法
US8560130B2 (en) 2007-12-04 2013-10-15 Lester F. Ludwig Software controlled lab-on-a-chip emulation
CN108088824B (zh) 2008-03-14 2021-06-04 雅培快速诊断耶拿有限公司 一种用于检测分析物的系统
EP2438196B1 (en) 2009-06-05 2016-12-21 Alere San Diego, Inc. Recombinase polymerase amplification reagents and kits
SG179200A1 (en) * 2009-09-16 2012-04-27 Univ Massey Fusion polypeptides and uses thereof
WO2013154898A1 (en) * 2012-04-12 2013-10-17 Ong, Jennifer Synthetic nucleic acids for polymerization reactions
US10533167B2 (en) * 2016-06-09 2020-01-14 The Trustees Of Princeton University Optogenetic tool for rapid and reversible clustering of proteins
WO2018100408A1 (en) * 2016-11-30 2018-06-07 Sasinapas Co.,Ltd. Modified peptides
US20210047659A1 (en) * 2018-01-24 2021-02-18 The Trustees Of Princeton University System and method for inducing clusters of gene regulatory proteins targeted to specific genomic loci

Also Published As

Publication number Publication date
CN114945682A (zh) 2022-08-26
AU2020382121A8 (en) 2022-07-28
GB201916379D0 (en) 2019-12-25
US11807899B2 (en) 2023-11-07
BR112022009072A2 (pt) 2022-07-26
US20220112547A1 (en) 2022-04-14
CA3157435A1 (en) 2021-05-20
WO2021094746A1 (en) 2021-05-20
JP2023501002A (ja) 2023-01-17
JP2026041819A (ja) 2026-03-10
JP7840266B2 (ja) 2026-04-03
MX2022005677A (es) 2022-09-07
ZA202205093B (en) 2024-10-30
US20220145378A1 (en) 2022-05-12
EP4058599A1 (en) 2022-09-21
IL292885A (en) 2022-07-01
KR20220113948A (ko) 2022-08-17

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Free format text: IN VOL 36 , NO 24 , PAGE(S) 3392 UNDER THE HEADING PCT APPLICATIONS THAT HAVE ENTERED THE NATIONAL PHASE - NAME INDEX UNDER THE NAME BIOCRUCIBLE LIMITED , APPLICATION NO. 2020382121, UNDER INID (71) CORRECT THE APPLICANT NAME TO BIOCRUCIBLE LTD.