AU2019409833B2 - Production of 3-fucosyllactose and lactose converting alpha-1,3-fucosyltransferase enzymes - Google Patents

Production of 3-fucosyllactose and lactose converting alpha-1,3-fucosyltransferase enzymes

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Publication number
AU2019409833B2
AU2019409833B2 AU2019409833A AU2019409833A AU2019409833B2 AU 2019409833 B2 AU2019409833 B2 AU 2019409833B2 AU 2019409833 A AU2019409833 A AU 2019409833A AU 2019409833 A AU2019409833 A AU 2019409833A AU 2019409833 B2 AU2019409833 B2 AU 2019409833B2
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AU
Australia
Prior art keywords
seq
polypeptide
lactose
fucosyllactose
cell
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AU2019409833A
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English (en)
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AU2019409833A1 (en
Inventor
Joeri Beauprez
Nausicaä LANNOO
Kristof VANDEWALLE
Annelies VERCAUTEREN
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Inbiose NV
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Inbiose NV
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Publication of AU2019409833A1 publication Critical patent/AU2019409833A1/en
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Publication of AU2019409833B2 publication Critical patent/AU2019409833B2/en
Priority to AU2026202319A priority Critical patent/AU2026202319A1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • C07H1/08—Separation; Purification from natural products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01065—3-Galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase (2.4.1.65), i.e. alpha-1-3 fucosyltransferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01152—4-Galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase (2.4.1.152)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Botany (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
AU2019409833A 2018-12-18 2019-12-18 Production of 3-fucosyllactose and lactose converting alpha-1,3-fucosyltransferase enzymes Active AU2019409833B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2026202319A AU2026202319A1 (en) 2018-12-18 2026-03-25 Production of 3-fucosyllactose and lactose converting alpha-1,3-fucosyltransferase enzymes

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP18213728 2018-12-18
EP18213728.1 2018-12-18
PCT/EP2019/085841 WO2020127417A2 (en) 2018-12-18 2019-12-18 PRODUCTION OF 3-FUCOSYLLACTOSE AND LACTOSE CONVERTING α-1,3-FUCOSYLTRANSFERASE ENZYMES

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2026202319A Division AU2026202319A1 (en) 2018-12-18 2026-03-25 Production of 3-fucosyllactose and lactose converting alpha-1,3-fucosyltransferase enzymes

Publications (2)

Publication Number Publication Date
AU2019409833A1 AU2019409833A1 (en) 2021-08-05
AU2019409833B2 true AU2019409833B2 (en) 2026-01-22

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AU2019409833A Active AU2019409833B2 (en) 2018-12-18 2019-12-18 Production of 3-fucosyllactose and lactose converting alpha-1,3-fucosyltransferase enzymes
AU2026202319A Pending AU2026202319A1 (en) 2018-12-18 2026-03-25 Production of 3-fucosyllactose and lactose converting alpha-1,3-fucosyltransferase enzymes

Family Applications After (1)

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Country Status (10)

Country Link
US (1) US20220002773A1 (https=)
EP (1) EP3898642A2 (https=)
JP (1) JP2022514743A (https=)
KR (1) KR102943876B1 (https=)
CN (1) CN113195509B (https=)
AU (2) AU2019409833B2 (https=)
BR (1) BR112021011298A2 (https=)
CA (1) CA3121848A1 (https=)
SG (1) SG11202106291RA (https=)
WO (1) WO2020127417A2 (https=)

Families Citing this family (19)

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Publication number Priority date Publication date Assignee Title
CN114438001B (zh) * 2020-11-05 2022-11-15 中国科学院上海高等研究院 生产3-岩藻糖基乳糖的重组枯草芽孢杆菌及其构建方法和应用
CN112662604B (zh) * 2020-12-29 2023-10-20 量子高科(广东)生物有限公司 一种合成3-岩藻糖基乳糖的重组大肠杆菌及其构建方法
EP4289958A4 (en) * 2021-02-08 2025-07-02 Kyowa Hakko Bio Co Ltd PROTEIN HAVING 1,3-FUCOSYLTRANSFERASE ACTIVITY, AND METHOD FOR PRODUCING FUCOSE-CONTAINING SUGAR
CN113648405B (zh) * 2021-08-19 2023-06-02 重庆医科大学 一种口服重组幽门螺杆菌蛋白疫苗纳米粒及其制备方法
KR20240114773A (ko) * 2021-12-14 2024-07-24 인바이오스 엔.브이. 알파-1,3-푸코실화된 화합물의 생산
US20240417768A1 (en) * 2021-12-14 2024-12-19 Inbiose N.V. Production of alpha-1,4-fucosylated compounds
CN115058465B (zh) * 2022-06-30 2025-03-07 山东大学 一种岩藻糖基化软骨素及其制备方法和应用
CN115287273A (zh) * 2022-06-30 2022-11-04 华熙生物科技股份有限公司 一种1，2-岩藻糖基转移酶及其融合蛋白和编码基因
KR20240017427A (ko) * 2022-07-29 2024-02-08 주식회사 진켐 3-푸코실락토스 생산용 유전자 조작 미생물 및 이를 이용한 방법
WO2024089131A1 (en) 2022-10-25 2024-05-02 Inbiose N.V. Saccharide importers for lacto-n-triose
WO2024153786A1 (en) 2023-01-19 2024-07-25 Inbiose N.V. Purification of an oligosaccharide or oligosaccharide mixture from a fermentation broth
WO2024153787A1 (en) 2023-01-19 2024-07-25 Inbiose N.V. Purification of a saccharide from a fermentation broth
WO2024153788A1 (en) 2023-01-19 2024-07-25 Inbiose N.V. Purification of an oligosaccharide or oligosaccharide mixture
CN116948928B (zh) * 2023-06-02 2024-06-18 虹摹生物科技(上海)有限公司 种子培养基及无抗生素、无iptg诱导剂的2’-岩藻糖基乳糖的发酵生产方法
CN116425810B (zh) * 2023-06-14 2023-08-11 山东合成远景生物科技有限公司 一种混合液中3-岩藻糖基乳糖的纯化方法
AU2024287855A1 (en) 2023-07-13 2026-02-26 Globachem N.V. Method to improve a plant's growth, development and resistance to (a)biotic stress
LU504742B1 (en) 2023-07-13 2025-01-14 Globachem N V Method to improve a plant's growth, development and resistance to (a)biotic stress
WO2025180259A1 (zh) * 2024-02-26 2025-09-04 恒睿（青岛）生物科技有限公司 岩藻糖基转移酶多肽及基因修饰细胞
CN118047820B (zh) * 2024-04-16 2024-07-19 北京三元食品股份有限公司 一种3-岩藻糖基乳糖的制备方法及制备的标准品

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WO2016040531A1 (en) * 2014-09-09 2016-03-17 Glycosyn LLC Alpha (1,3) fucosyltransferases for use in the production of fucosylated oligosaccharides

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AU8005098A (en) 1997-06-06 1998-12-21 Governors Of The University Of Alberta, The Alpha1,3-fucosyltransferase of helicobacter pylori
SG10201504733XA (en) * 2010-07-12 2015-07-30 Univ Gent Metabolically engineered organisms for the production of added value bio-products
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CN108103039B (zh) * 2016-11-25 2021-10-22 上海交通大学 一组岩藻糖基转移酶突变体及其筛选方法和应用
WO2021013708A1 (en) * 2019-07-19 2021-01-28 Inbiose N.V. Production of fucosyllactose in host cells

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WO2016040531A1 (en) * 2014-09-09 2016-03-17 Glycosyn LLC Alpha (1,3) fucosyltransferases for use in the production of fucosylated oligosaccharides

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Also Published As

Publication number Publication date
KR20210107736A (ko) 2021-09-01
WO2020127417A3 (en) 2020-07-30
CN113195509B (zh) 2025-02-21
JP2022514743A (ja) 2022-02-15
CA3121848A1 (en) 2020-06-25
KR102943876B1 (ko) 2026-03-24
CN113195509A (zh) 2021-07-30
BR112021011298A2 (pt) 2021-11-30
US20220002773A1 (en) 2022-01-06
SG11202106291RA (en) 2021-07-29
WO2020127417A2 (en) 2020-06-25
AU2019409833A1 (en) 2021-08-05
AU2026202319A1 (en) 2026-04-16
EP3898642A2 (en) 2021-10-27

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