CA3121848A1 - Production of 3-fucosyllactose and lactose converting .alpha.-1,3-fucosyltransferase enzymes - Google Patents
Production of 3-fucosyllactose and lactose converting .alpha.-1,3-fucosyltransferase enzymes Download PDFInfo
- Publication number
- CA3121848A1 CA3121848A1 CA3121848A CA3121848A CA3121848A1 CA 3121848 A1 CA3121848 A1 CA 3121848A1 CA 3121848 A CA3121848 A CA 3121848A CA 3121848 A CA3121848 A CA 3121848A CA 3121848 A1 CA3121848 A1 CA 3121848A1
- Authority
- CA
- Canada
- Prior art keywords
- seq
- lactose
- fucosyllactose
- polypeptide
- amino acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
- C07H1/08—Separation; Purification from natural products
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
- C12Y204/01065—3-Galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase (2.4.1.65), i.e. alpha-1-3 fucosyltransferase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
- C12Y204/01152—4-Galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase (2.4.1.152)
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Botany (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP18213728 | 2018-12-18 | ||
| EP18213728.1 | 2018-12-18 | ||
| PCT/EP2019/085841 WO2020127417A2 (en) | 2018-12-18 | 2019-12-18 | PRODUCTION OF 3-FUCOSYLLACTOSE AND LACTOSE CONVERTING α-1,3-FUCOSYLTRANSFERASE ENZYMES |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3121848A1 true CA3121848A1 (en) | 2020-06-25 |
Family
ID=64745987
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3121848A Pending CA3121848A1 (en) | 2018-12-18 | 2019-12-18 | Production of 3-fucosyllactose and lactose converting .alpha.-1,3-fucosyltransferase enzymes |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US20220002773A1 (https=) |
| EP (1) | EP3898642A2 (https=) |
| JP (1) | JP2022514743A (https=) |
| KR (1) | KR102943876B1 (https=) |
| CN (1) | CN113195509B (https=) |
| AU (2) | AU2019409833B2 (https=) |
| BR (1) | BR112021011298A2 (https=) |
| CA (1) | CA3121848A1 (https=) |
| SG (1) | SG11202106291RA (https=) |
| WO (1) | WO2020127417A2 (https=) |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114438001B (zh) * | 2020-11-05 | 2022-11-15 | 中国科学院上海高等研究院 | 生产3-岩藻糖基乳糖的重组枯草芽孢杆菌及其构建方法和应用 |
| CN112662604B (zh) * | 2020-12-29 | 2023-10-20 | 量子高科(广东)生物有限公司 | 一种合成3-岩藻糖基乳糖的重组大肠杆菌及其构建方法 |
| EP4289958A4 (en) * | 2021-02-08 | 2025-07-02 | Kyowa Hakko Bio Co Ltd | PROTEIN HAVING 1,3-FUCOSYLTRANSFERASE ACTIVITY, AND METHOD FOR PRODUCING FUCOSE-CONTAINING SUGAR |
| CN113648405B (zh) * | 2021-08-19 | 2023-06-02 | 重庆医科大学 | 一种口服重组幽门螺杆菌蛋白疫苗纳米粒及其制备方法 |
| KR20240114773A (ko) * | 2021-12-14 | 2024-07-24 | 인바이오스 엔.브이. | 알파-1,3-푸코실화된 화합물의 생산 |
| US20240417768A1 (en) * | 2021-12-14 | 2024-12-19 | Inbiose N.V. | Production of alpha-1,4-fucosylated compounds |
| CN115058465B (zh) * | 2022-06-30 | 2025-03-07 | 山东大学 | 一种岩藻糖基化软骨素及其制备方法和应用 |
| CN115287273A (zh) * | 2022-06-30 | 2022-11-04 | 华熙生物科技股份有限公司 | 一种1，2-岩藻糖基转移酶及其融合蛋白和编码基因 |
| KR20240017427A (ko) * | 2022-07-29 | 2024-02-08 | 주식회사 진켐 | 3-푸코실락토스 생산용 유전자 조작 미생물 및 이를 이용한 방법 |
| WO2024089131A1 (en) | 2022-10-25 | 2024-05-02 | Inbiose N.V. | Saccharide importers for lacto-n-triose |
| WO2024153786A1 (en) | 2023-01-19 | 2024-07-25 | Inbiose N.V. | Purification of an oligosaccharide or oligosaccharide mixture from a fermentation broth |
| WO2024153787A1 (en) | 2023-01-19 | 2024-07-25 | Inbiose N.V. | Purification of a saccharide from a fermentation broth |
| WO2024153788A1 (en) | 2023-01-19 | 2024-07-25 | Inbiose N.V. | Purification of an oligosaccharide or oligosaccharide mixture |
| CN116948928B (zh) * | 2023-06-02 | 2024-06-18 | 虹摹生物科技(上海)有限公司 | 种子培养基及无抗生素、无iptg诱导剂的2’-岩藻糖基乳糖的发酵生产方法 |
| CN116425810B (zh) * | 2023-06-14 | 2023-08-11 | 山东合成远景生物科技有限公司 | 一种混合液中3-岩藻糖基乳糖的纯化方法 |
| AU2024287855A1 (en) | 2023-07-13 | 2026-02-26 | Globachem N.V. | Method to improve a plant's growth, development and resistance to (a)biotic stress |
| LU504742B1 (en) | 2023-07-13 | 2025-01-14 | Globachem N V | Method to improve a plant's growth, development and resistance to (a)biotic stress |
| WO2025180259A1 (zh) * | 2024-02-26 | 2025-09-04 | 恒睿（青岛）生物科技有限公司 | 岩藻糖基转移酶多肽及基因修饰细胞 |
| CN118047820B (zh) * | 2024-04-16 | 2024-07-19 | 北京三元食品股份有限公司 | 一种3-岩藻糖基乳糖的制备方法及制备的标准品 |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU8005098A (en) | 1997-06-06 | 1998-12-21 | Governors Of The University Of Alberta, The | Alpha1,3-fucosyltransferase of helicobacter pylori |
| SG10201504733XA (en) * | 2010-07-12 | 2015-07-30 | Univ Gent | Metabolically engineered organisms for the production of added value bio-products |
| EP2944690B1 (en) * | 2010-10-11 | 2017-12-20 | Jennewein Biotechnologie GmbH | Novel fucosyltransferases and their applications |
| ES3002778T3 (en) | 2014-06-27 | 2025-03-07 | Glycom As | Oligosaccharide production |
| AU2015315110B2 (en) * | 2014-09-09 | 2021-04-08 | Glycosyn LLC | Alpha (1,3) fucosyltransferases for use in the production of fucosylated oligosaccharides |
| BR112017006295B1 (pt) * | 2014-11-14 | 2023-10-17 | Inbiose N.V | Método para a produção de microrganismos mutantes resistentes à morte por lactose, microrganismo e usos do mesmo |
| ES2856749T3 (es) | 2016-10-29 | 2021-09-28 | Chr Hansen Hmo Gmbh | Proceso para la producción de oligosacáridos fucosilados |
| CN108103039B (zh) * | 2016-11-25 | 2021-10-22 | 上海交通大学 | 一组岩藻糖基转移酶突变体及其筛选方法和应用 |
| WO2021013708A1 (en) * | 2019-07-19 | 2021-01-28 | Inbiose N.V. | Production of fucosyllactose in host cells |
-
2019
- 2019-12-18 JP JP2021535101A patent/JP2022514743A/ja active Pending
- 2019-12-18 AU AU2019409833A patent/AU2019409833B2/en active Active
- 2019-12-18 BR BR112021011298A patent/BR112021011298A2/pt not_active IP Right Cessation
- 2019-12-18 EP EP19832061.6A patent/EP3898642A2/en active Pending
- 2019-12-18 CA CA3121848A patent/CA3121848A1/en active Pending
- 2019-12-18 CN CN201980083180.5A patent/CN113195509B/zh active Active
- 2019-12-18 WO PCT/EP2019/085841 patent/WO2020127417A2/en not_active Ceased
- 2019-12-18 KR KR1020217022575A patent/KR102943876B1/ko active Active
- 2019-12-18 US US17/295,688 patent/US20220002773A1/en active Pending
- 2019-12-18 SG SG11202106291RA patent/SG11202106291RA/en unknown
-
2026
- 2026-03-25 AU AU2026202319A patent/AU2026202319A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| KR20210107736A (ko) | 2021-09-01 |
| WO2020127417A3 (en) | 2020-07-30 |
| CN113195509B (zh) | 2025-02-21 |
| JP2022514743A (ja) | 2022-02-15 |
| KR102943876B1 (ko) | 2026-03-24 |
| CN113195509A (zh) | 2021-07-30 |
| BR112021011298A2 (pt) | 2021-11-30 |
| US20220002773A1 (en) | 2022-01-06 |
| SG11202106291RA (en) | 2021-07-29 |
| WO2020127417A2 (en) | 2020-06-25 |
| AU2019409833A1 (en) | 2021-08-05 |
| AU2019409833B2 (en) | 2026-01-22 |
| AU2026202319A1 (en) | 2026-04-16 |
| EP3898642A2 (en) | 2021-10-27 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20220923 |
|
| W00 | Other event occurred |
Free format text: ST27 STATUS EVENT CODE: A-2-2-W10-W00-W100 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: LETTER SENT Effective date: 20250902 |
|
| U13 | Renewal or maintenance fee not paid |
Free format text: ST27 STATUS EVENT CODE: N-2-6-U10-U13-U300 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: DEEMED ABANDONED - FAILURE TO RESPOND TO MAINTENANCE FEE NOTICE Effective date: 20251103 |
|
| W00 | Other event occurred |
Free format text: ST27 STATUS EVENT CODE: N-6-6-W10-W00-W100 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: LETTER SENT Effective date: 20260226 |