KR102943876B1 - 3-푸코실락토스 및 락토스 전환 α-1,3-푸코실트란스퍼라제 효소를 제조하는 방법 - Google Patents

3-푸코실락토스 및 락토스 전환 α-1,3-푸코실트란스퍼라제 효소를 제조하는 방법

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KR102943876B1
KR102943876B1 KR1020217022575A KR20217022575A KR102943876B1 KR 102943876 B1 KR102943876 B1 KR 102943876B1 KR 1020217022575 A KR1020217022575 A KR 1020217022575A KR 20217022575 A KR20217022575 A KR 20217022575A KR 102943876 B1 KR102943876 B1 KR 102943876B1
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조에리 보우프레즈
나우시카 란누
크리스토프 반데발레
아넬리스 베르카투른
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인바이오스 엔.브이.
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    • C—CHEMISTRY; METALLURGY
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    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C—CHEMISTRY; METALLURGY
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    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
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    • C12N1/14—Fungi; Culture media therefor
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
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    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
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    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01065—3-Galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase (2.4.1.65), i.e. alpha-1-3 fucosyltransferase
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    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01152—4-Galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase (2.4.1.152)

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  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
KR1020217022575A 2018-12-18 2019-12-18 3-푸코실락토스 및 락토스 전환 α-1,3-푸코실트란스퍼라제 효소를 제조하는 방법 Active KR102943876B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP18213728 2018-12-18
EP18213728.1 2018-12-18
PCT/EP2019/085841 WO2020127417A2 (en) 2018-12-18 2019-12-18 PRODUCTION OF 3-FUCOSYLLACTOSE AND LACTOSE CONVERTING α-1,3-FUCOSYLTRANSFERASE ENZYMES

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Publication Number Publication Date
KR20210107736A KR20210107736A (ko) 2021-09-01
KR102943876B1 true KR102943876B1 (ko) 2026-03-24

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Country Link
US (1) US20220002773A1 (https=)
EP (1) EP3898642A2 (https=)
JP (1) JP2022514743A (https=)
KR (1) KR102943876B1 (https=)
CN (1) CN113195509B (https=)
AU (2) AU2019409833B2 (https=)
BR (1) BR112021011298A2 (https=)
CA (1) CA3121848A1 (https=)
SG (1) SG11202106291RA (https=)
WO (1) WO2020127417A2 (https=)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114438001B (zh) * 2020-11-05 2022-11-15 中国科学院上海高等研究院 生产3-岩藻糖基乳糖的重组枯草芽孢杆菌及其构建方法和应用
CN112662604B (zh) * 2020-12-29 2023-10-20 量子高科(广东)生物有限公司 一种合成3-岩藻糖基乳糖的重组大肠杆菌及其构建方法
EP4289958A4 (en) * 2021-02-08 2025-07-02 Kyowa Hakko Bio Co Ltd PROTEIN HAVING 1,3-FUCOSYLTRANSFERASE ACTIVITY, AND METHOD FOR PRODUCING FUCOSE-CONTAINING SUGAR
CN113648405B (zh) * 2021-08-19 2023-06-02 重庆医科大学 一种口服重组幽门螺杆菌蛋白疫苗纳米粒及其制备方法
KR20240114773A (ko) * 2021-12-14 2024-07-24 인바이오스 엔.브이. 알파-1,3-푸코실화된 화합물의 생산
US20240417768A1 (en) * 2021-12-14 2024-12-19 Inbiose N.V. Production of alpha-1,4-fucosylated compounds
CN115058465B (zh) * 2022-06-30 2025-03-07 山东大学 一种岩藻糖基化软骨素及其制备方法和应用
CN115287273A (zh) * 2022-06-30 2022-11-04 华熙生物科技股份有限公司 一种1，2-岩藻糖基转移酶及其融合蛋白和编码基因
KR20240017427A (ko) * 2022-07-29 2024-02-08 주식회사 진켐 3-푸코실락토스 생산용 유전자 조작 미생물 및 이를 이용한 방법
WO2024089131A1 (en) 2022-10-25 2024-05-02 Inbiose N.V. Saccharide importers for lacto-n-triose
WO2024153786A1 (en) 2023-01-19 2024-07-25 Inbiose N.V. Purification of an oligosaccharide or oligosaccharide mixture from a fermentation broth
WO2024153787A1 (en) 2023-01-19 2024-07-25 Inbiose N.V. Purification of a saccharide from a fermentation broth
WO2024153788A1 (en) 2023-01-19 2024-07-25 Inbiose N.V. Purification of an oligosaccharide or oligosaccharide mixture
CN116948928B (zh) * 2023-06-02 2024-06-18 虹摹生物科技(上海)有限公司 种子培养基及无抗生素、无iptg诱导剂的2’-岩藻糖基乳糖的发酵生产方法
CN116425810B (zh) * 2023-06-14 2023-08-11 山东合成远景生物科技有限公司 一种混合液中3-岩藻糖基乳糖的纯化方法
AU2024287855A1 (en) 2023-07-13 2026-02-26 Globachem N.V. Method to improve a plant's growth, development and resistance to (a)biotic stress
LU504742B1 (en) 2023-07-13 2025-01-14 Globachem N V Method to improve a plant's growth, development and resistance to (a)biotic stress
WO2025180259A1 (zh) * 2024-02-26 2025-09-04 恒睿（青岛）生物科技有限公司 岩藻糖基转移酶多肽及基因修饰细胞
CN118047820B (zh) * 2024-04-16 2024-07-19 北京三元食品股份有限公司 一种3-岩藻糖基乳糖的制备方法及制备的标准品

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015197082A1 (en) 2014-06-27 2015-12-30 Glycom A/S Oligosaccharide production
JP2017527311A (ja) * 2014-09-09 2017-09-21 グリコシン リミテッド ライアビリティー カンパニー フコシル化オリゴ糖の生産において使用するためのα（１，３）フコシルトランスフェラーゼ
WO2018077892A1 (en) 2016-10-29 2018-05-03 Jennewein Biotechnologie Gmbh Improved process for the production of fucosylated oligosaccharides

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU8005098A (en) 1997-06-06 1998-12-21 Governors Of The University Of Alberta, The Alpha1,3-fucosyltransferase of helicobacter pylori
SG10201504733XA (en) * 2010-07-12 2015-07-30 Univ Gent Metabolically engineered organisms for the production of added value bio-products
EP2944690B1 (en) * 2010-10-11 2017-12-20 Jennewein Biotechnologie GmbH Novel fucosyltransferases and their applications
BR112017006295B1 (pt) * 2014-11-14 2023-10-17 Inbiose N.V Método para a produção de microrganismos mutantes resistentes à morte por lactose, microrganismo e usos do mesmo
CN108103039B (zh) * 2016-11-25 2021-10-22 上海交通大学 一组岩藻糖基转移酶突变体及其筛选方法和应用
WO2021013708A1 (en) * 2019-07-19 2021-01-28 Inbiose N.V. Production of fucosyllactose in host cells

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015197082A1 (en) 2014-06-27 2015-12-30 Glycom A/S Oligosaccharide production
JP2017527311A (ja) * 2014-09-09 2017-09-21 グリコシン リミテッド ライアビリティー カンパニー フコシル化オリゴ糖の生産において使用するためのα（１，３）フコシルトランスフェラーゼ
WO2018077892A1 (en) 2016-10-29 2018-05-03 Jennewein Biotechnologie Gmbh Improved process for the production of fucosylated oligosaccharides

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
NCBI Reference Sequence: WP_042442472.1(2015.02.09.)*

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KR20210107736A (ko) 2021-09-01
WO2020127417A3 (en) 2020-07-30
CN113195509B (zh) 2025-02-21
JP2022514743A (ja) 2022-02-15
CA3121848A1 (en) 2020-06-25
CN113195509A (zh) 2021-07-30
BR112021011298A2 (pt) 2021-11-30
US20220002773A1 (en) 2022-01-06
SG11202106291RA (en) 2021-07-29
WO2020127417A2 (en) 2020-06-25
AU2019409833A1 (en) 2021-08-05
AU2019409833B2 (en) 2026-01-22
AU2026202319A1 (en) 2026-04-16
EP3898642A2 (en) 2021-10-27

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