AU2019242331A1 - Method for producing hairpin single-stranded RNA molecule - Google Patents

Method for producing hairpin single-stranded RNA molecule Download PDF

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Publication number
AU2019242331A1
AU2019242331A1 AU2019242331A AU2019242331A AU2019242331A1 AU 2019242331 A1 AU2019242331 A1 AU 2019242331A1 AU 2019242331 A AU2019242331 A AU 2019242331A AU 2019242331 A AU2019242331 A AU 2019242331A AU 2019242331 A1 AU2019242331 A1 AU 2019242331A1
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Australia
Prior art keywords
linker
stranded oligorna
stranded
seq
connected via
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AU2019242331A
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Inventor
Hideaki Inada
Katsuhiko Iseki
Keiichi OKIMURA
Masato Sanosaka
Ayumi Takashina
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Toray Industries Inc
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Toray Industries Inc
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Publication of AU2019242331A1 publication Critical patent/AU2019242331A1/en
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12YENZYMES
    • C12Y605/00Ligases forming phosphoric ester bonds (6.5)
    • C12Y605/01Ligases forming phosphoric ester bonds (6.5) forming phosphoric ester bonds (6.5.1)
    • C12Y605/01003RNA ligase (ATP) (6.5.1.3)
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111General methods applicable to biologically active non-coding nucleic acids
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1136Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against growth factors, growth regulators, cytokines, lymphokines or hormones
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    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/26Preparation of nitrogen-containing carbohydrates
    • C12P19/28N-glycosides
    • C12P19/30Nucleotides
    • C12P19/34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • CCHEMISTRY; METALLURGY
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    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/10Type of nucleic acid
    • C12N2310/12Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
    • C12N2310/122Hairpin
    • CCHEMISTRY; METALLURGY
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    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/10Type of nucleic acid
    • C12N2310/14Type of nucleic acid interfering N.A.
    • CCHEMISTRY; METALLURGY
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    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/30Chemical structure
    • C12N2310/31Chemical structure of the backbone
    • C12N2310/318Chemical structure of the backbone where the PO2 is completely replaced, e.g. MMI or formacetal
    • CCHEMISTRY; METALLURGY
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    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/50Physical structure
    • C12N2310/53Physical structure partially self-complementary or closed
    • C12N2310/531Stem-loop; Hairpin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2330/00Production
    • C12N2330/30Production chemically synthesised

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
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  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Endocrinology (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AU2019242331A 2018-03-30 2019-03-29 Method for producing hairpin single-stranded RNA molecule Pending AU2019242331A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2018070423 2018-03-30
JP2018-070423 2018-03-30
PCT/JP2019/013923 WO2019189722A1 (ja) 2018-03-30 2019-03-29 ヘアピン型一本鎖rna分子の製造方法

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AU2019242331A1 true AU2019242331A1 (en) 2020-09-10

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ID=68061953

Family Applications (1)

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AU2019242331A Pending AU2019242331A1 (en) 2018-03-30 2019-03-29 Method for producing hairpin single-stranded RNA molecule

Country Status (15)

Country Link
US (2) US11920131B2 (ko)
EP (1) EP3778886A4 (ko)
JP (2) JP6631751B1 (ko)
KR (1) KR20200136363A (ko)
CN (1) CN111819280A (ko)
AU (1) AU2019242331A1 (ko)
BR (1) BR112020017769A2 (ko)
CA (1) CA3094160A1 (ko)
IL (1) IL277346A (ko)
MX (1) MX2020009556A (ko)
PH (1) PH12020551487A1 (ko)
RU (1) RU2020130258A (ko)
TW (1) TWI772632B (ko)
WO (1) WO2019189722A1 (ko)
ZA (1) ZA202005149B (ko)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3862431B1 (en) * 2018-10-02 2024-06-26 Toray Industries, Inc. Method for producing hairpin single-strand rna molecules
WO2021113494A1 (en) * 2019-12-03 2021-06-10 Beam Therapeutics Inc. Synthetic guide rna, compositions, methods, and uses thereof
US20230167152A1 (en) * 2020-03-27 2023-06-01 Sumitomo Chemical Company, Limited Method for producing nucleic acid oligomer
CA3229816A1 (en) * 2021-08-27 2023-03-02 Wensheng Wei Constructs and methods for preparing circular rna
CN117660374A (zh) * 2022-09-08 2024-03-08 凯莱英医药集团(天津)股份有限公司 Rna连接酶在寡核苷酸制备上的应用
CN115774075B (zh) * 2023-02-15 2023-06-06 江苏耀海生物制药有限公司 一种基于RP-HPLC分析体外转录产物成分circRNA的方法

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EP1238105A2 (en) * 1999-12-02 2002-09-11 Molecular Staging Inc. Generation of single-strand circular dna from linear self-annealing segments
US20040058886A1 (en) * 2002-08-08 2004-03-25 Dharmacon, Inc. Short interfering RNAs having a hairpin structure containing a non-nucleotide loop
JP5296328B2 (ja) 2007-05-09 2013-09-25 独立行政法人理化学研究所 1本鎖環状rnaおよびその製造方法
US20110055965A1 (en) * 2008-02-15 2011-03-03 Hiroshi Abe Cycle single-stranded nucleic acid complex and method for producing the same
WO2011052013A1 (ja) 2009-10-29 2011-05-05 株式会社バイオダイナミクス研究所 核酸鎖の結合および修飾方法
TWI573870B (zh) 2010-07-08 2017-03-11 Bonac Corp Single-stranded nucleic acid molecules used to control gene expression (I)
EP2674494B1 (en) 2010-08-03 2014-12-17 Bonac Corporation Single-stranded RNA molecule having nitrogen-containing alicyclic skeleton
KR101761103B1 (ko) 2011-08-25 2017-07-25 가부시키가이샤 보낙 글리코시드 화합물, 티오에테르의 제조방법, 에테르, 에테르의 제조방법, 글리코시드 화합물의 제조방법, 핵산의 제조방법
WO2013077446A1 (ja) * 2011-11-26 2013-05-30 株式会社ボナック 遺伝子発現制御のための一本鎖核酸分子
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TWI830718B (zh) 2018-02-09 2024-02-01 日商住友化學股份有限公司 核酸分子之製造方法
JP6817493B2 (ja) * 2018-03-30 2021-01-20 住友化学株式会社 一本鎖rnaの製造方法

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Publication number Publication date
ZA202005149B (en) 2022-01-26
US20210024930A1 (en) 2021-01-28
JPWO2019189722A1 (ja) 2020-04-30
MX2020009556A (es) 2020-10-05
KR20200136363A (ko) 2020-12-07
PH12020551487A1 (en) 2021-08-23
JP2019213567A (ja) 2019-12-19
EP3778886A1 (en) 2021-02-17
JP6631751B1 (ja) 2020-01-15
CN111819280A (zh) 2020-10-23
US20240167033A1 (en) 2024-05-23
CA3094160A1 (en) 2019-10-03
EP3778886A4 (en) 2022-11-02
BR112020017769A2 (pt) 2021-01-05
US11920131B2 (en) 2024-03-05
WO2019189722A1 (ja) 2019-10-03
TWI772632B (zh) 2022-08-01
JP7363316B2 (ja) 2023-10-18
IL277346A (en) 2020-10-29
RU2020130258A (ru) 2022-05-04
TW202003842A (zh) 2020-01-16

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