AU2015223025B2 - Enzymatic hydrolysis of disaccharides and oligosaccharides using alpha-glucosidase enzymes - Google Patents

Enzymatic hydrolysis of disaccharides and oligosaccharides using alpha-glucosidase enzymes Download PDF

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AU2015223025B2
AU2015223025B2 AU2015223025A AU2015223025A AU2015223025B2 AU 2015223025 B2 AU2015223025 B2 AU 2015223025B2 AU 2015223025 A AU2015223025 A AU 2015223025A AU 2015223025 A AU2015223025 A AU 2015223025A AU 2015223025 B2 AU2015223025 B2 AU 2015223025B2
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AU2015223025A1 (en
Inventor
Robert Dicosimo
Erwin Columbus HAGO
Susan Marie Hennessey
Ling Hua
Kevin D. Nagy
Rodrigo Ramirez
Jayarama K. Shetty
Zhongmei TANG
Zheyong YU
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Nutrition and Biosciences USA 4 Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/16—Preparation of compounds containing saccharide radicals produced by the action of an alpha-1, 6-glucosidase, e.g. amylose, debranched amylopectin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H3/00—Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
    • C07H3/06—Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C12P19/08—Dextran
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/12—Disaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01005—Dextransucrase (2.4.1.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01024—1,4-Alpha-glucan 6-alpha-glucosyltransferase (2.4.1.24)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/0102—Alpha-glucosidase (3.2.1.20)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/0107—Glucan 1,6-alpha-glucosidase (3.2.1.70)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01084—Glucan 1,3-alpha-glucosidase (3.2.1.84), i.e. mutanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01115—Branched-dextran exo-1,2-alpha-glucosidase (3.2.1.115)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU2015223025A 2014-02-27 2015-02-26 Enzymatic hydrolysis of disaccharides and oligosaccharides using alpha-glucosidase enzymes Active AU2015223025B2 (en)

Applications Claiming Priority (19)

Application Number Priority Date Filing Date Title
US201461945233P 2014-02-27 2014-02-27
US201461945241P 2014-02-27 2014-02-27
US61/945,233 2014-02-27
US61/945,241 2014-02-27
US201462004312P 2014-05-29 2014-05-29
US201462004290P 2014-05-29 2014-05-29
US201462004314P 2014-05-29 2014-05-29
US201462004308P 2014-05-29 2014-05-29
US201462004305P 2014-05-29 2014-05-29
US201462004300P 2014-05-29 2014-05-29
US62/004,314 2014-05-29
US62/004,305 2014-05-29
US62/004,300 2014-05-29
US62/004,312 2014-05-29
US62/004,290 2014-05-29
US62/004,308 2014-05-29
CN2015073269 2015-02-25
CNPCT/CN2015/073269 2015-02-25
PCT/US2015/017644 WO2015130881A1 (en) 2014-02-27 2015-02-26 Enzymatic hydrolysis of disaccharides and oligosaccharides using alpha-glucosidase enzymes

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AU2015223025A1 AU2015223025A1 (en) 2016-08-04
AU2015223025B2 true AU2015223025B2 (en) 2018-09-20

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AU2015223027A Active AU2015223027B2 (en) 2014-02-27 2015-02-26 Enzymatic hydrolysis of disaccharides and oligosaccharides using alpha-glucosidase enzymes

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EP (2) EP3110959A1 (cg-RX-API-DMAC7.html)
JP (2) JP6929499B2 (cg-RX-API-DMAC7.html)
CN (2) CN106068327A (cg-RX-API-DMAC7.html)
AU (2) AU2015223025B2 (cg-RX-API-DMAC7.html)
BR (1) BR112016019823B1 (cg-RX-API-DMAC7.html)
CA (2) CA2938144A1 (cg-RX-API-DMAC7.html)
WO (2) WO2015130881A1 (cg-RX-API-DMAC7.html)

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JP6785242B2 (ja) 2014-12-22 2020-11-18 デュポン・インダストリアル・バイオサイエンシーズ・ユーエスエイ・エルエルシー ポリアルファ−１，３−グルカンを含有するポリマーブレンド
CN107205910A (zh) 2015-02-06 2017-09-26 纳幕尔杜邦公司 基于聚α‑1,3‑葡聚糖的聚合物的胶体分散体
WO2016138315A1 (en) * 2015-02-25 2016-09-01 Danisco Us Inc Alpha-glucosidase, compositions & methods
JP6956013B2 (ja) 2015-06-01 2021-10-27 ニュートリション・アンド・バイオサイエンシーズ・ユーエスエー・フォー，インコーポレイテッド ポリα−１，３−グルカンフィブリッド及びその使用、並びにポリα−１，３−グルカンフィブリッドを製造する方法
WO2016196021A1 (en) 2015-06-01 2016-12-08 E I Du Pont De Nemours And Company Structured liquid compositions comprising colloidal dispersions of poly alpha-1,3-glucan
RU2729250C2 (ru) 2015-10-26 2020-08-05 ДЮПОН ИНДАСТРИАЛ БАЙОСАЙЕНСИЗ ЮЭсЭй, ЭлЭлСи Полисахаридные покрытия для бумаги
ES2937288T3 (es) 2015-10-26 2023-03-27 Nutrition & Biosciences Usa 4 Inc Composición de alfa-(1,3->glucano) insoluble en agua
WO2017083226A1 (en) 2015-11-13 2017-05-18 E. I. Du Pont De Nemours And Company Glucan fiber compositions for use in laundry care and fabric care
WO2017083229A1 (en) * 2015-11-13 2017-05-18 E. I. Du Pont De Nemours And Company Glucan fiber compositions for use in laundry care and fabric care
EP3374400B1 (en) * 2015-11-13 2022-04-13 Nutrition & Biosciences USA 4, Inc. Glucan fiber compositions for use in laundry care and fabric care
US10266861B2 (en) * 2015-12-14 2019-04-23 E. I. Du Pont De Nemours And Company Production and composition of fructose syrup
US10895028B2 (en) 2015-12-14 2021-01-19 Dupont Industrial Biosciences Usa, Llc Nonwoven glucan webs
US20200095614A1 (en) * 2016-12-15 2020-03-26 Danisco Us Inc. Method for increasing the production of ethanol from corn fiber in a starch hydrolysis process
JP2018139554A (ja) * 2017-02-28 2018-09-13 日本食品化工株式会社 水溶性食物繊維含有組成物及びその製造方法
AU2018272823B2 (en) 2017-05-23 2024-05-09 Nutrition & Biosciences USA 4, Inc. Enzymatic production of alpha-1,3-glucan
EP3415622A1 (en) * 2017-06-14 2018-12-19 Evonik Degussa GmbH Method for production of fine chemicals using a corynebacterium secreting modified alpha-1,6-glucosidases
EP3668970A1 (en) * 2017-09-13 2020-06-24 DuPont Industrial Biosciences USA, LLC Engineered glucosyltransferases
CN111566128B (zh) * 2017-11-10 2022-06-21 营养与生物科学美国4公司 独特形态的多糖
EP3740583A1 (en) * 2018-02-23 2020-11-25 Danisco US Inc. Synthesis of glucan comprising alpha-1,3 glycosidic linkages with phosphorylase enzymes
CN115197884A (zh) * 2022-08-24 2022-10-18 吉林大学 一种降解废弃淀粉的微生物菌剂及其应用方法
CN120118963A (zh) * 2023-12-08 2025-06-10 宁夏伊品生物科技股份有限公司 aglA基因在制备L-氨基酸中的应用

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EP3110958A1 (en) 2017-01-04
BR112016019820A2 (pt) 2017-10-17
JP6929499B2 (ja) 2021-09-01
EP3110959A1 (en) 2017-01-04
CN106068327A (zh) 2016-11-02
AU2015223027B2 (en) 2018-09-20
JP7011393B2 (ja) 2022-02-10
BR112016019823B1 (pt) 2023-05-09
CA2938144A1 (en) 2015-09-03
BR112016019823A2 (pt) 2017-10-17
JP2017518026A (ja) 2017-07-06
CN106460023A (zh) 2017-02-22
WO2015130883A9 (en) 2016-08-18
AU2015223027A1 (en) 2016-08-04
WO2015130883A1 (en) 2015-09-03
CA2940778A1 (en) 2015-09-03
AU2015223025A1 (en) 2016-08-04
JP2017518025A (ja) 2017-07-06
WO2015130881A1 (en) 2015-09-03

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