AU2013336430B2 - Droplet interfaces - Google Patents

Droplet interfaces Download PDF

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Publication number
AU2013336430B2
AU2013336430B2 AU2013336430A AU2013336430A AU2013336430B2 AU 2013336430 B2 AU2013336430 B2 AU 2013336430B2 AU 2013336430 A AU2013336430 A AU 2013336430A AU 2013336430 A AU2013336430 A AU 2013336430A AU 2013336430 B2 AU2013336430 B2 AU 2013336430B2
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medium
polar medium
volume
outer hydrophilic
membrane
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AU2013336430A1 (en
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Clive Gavin Brown
Andrew John Heron
Jason Robert Hyde
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Oxford Nanopore Technologies PLC
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Oxford Nanopore Technologies PLC
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483Physical analysis of biological material
    • G01N33/487Physical analysis of biological material of liquid biological material
    • G01N33/48707Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721Investigating individual macromolecules, e.g. by translocation through nanopores
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D67/00Processes specially adapted for manufacturing semi-permeable membranes for separation processes or apparatus
    • B01D67/0002Organic membrane manufacture
    • B01D67/0023Organic membrane manufacture by inducing porosity into non porous precursor membranes
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D69/00Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor
    • B01D69/12Composite membranes; Ultra-thin membranes
    • B01D69/122Separate manufacturing of ultra-thin membranes
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D71/00Semi-permeable membranes for separation processes or apparatus characterised by the material; Manufacturing processes specially adapted therefor
    • B01D71/06Organic material
    • B01D71/26Polyalkenes
    • B01D71/261Polyethylene
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D71/00Semi-permeable membranes for separation processes or apparatus characterised by the material; Manufacturing processes specially adapted therefor
    • B01D71/06Organic material
    • B01D71/26Polyalkenes
    • B01D71/262Polypropylene
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D71/00Semi-permeable membranes for separation processes or apparatus characterised by the material; Manufacturing processes specially adapted therefor
    • B01D71/06Organic material
    • B01D71/70Polymers having silicon in the main chain, with or without sulfur, nitrogen, oxygen or carbon only
    • B01D71/701Polydimethylsiloxane
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D71/00Semi-permeable membranes for separation processes or apparatus characterised by the material; Manufacturing processes specially adapted therefor
    • B01D71/06Organic material
    • B01D71/76Macromolecular material not specifically provided for in a single one of groups B01D71/08 - B01D71/74
    • B01D71/80Block polymers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D2323/00Details relating to membrane preparation
    • B01D2323/35Use of magnetic or electrical fields
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D2325/00Details relating to properties of membranes
    • B01D2325/36Hydrophilic membranes
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D2325/00Details relating to properties of membranes
    • B01D2325/38Hydrophobic membranes
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D71/00Semi-permeable membranes for separation processes or apparatus characterised by the material; Manufacturing processes specially adapted therefor
    • B01D71/06Organic material
    • B01D71/30Polyalkenyl halides
    • B01D71/32Polyalkenyl halides containing fluorine atoms
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D71/00Semi-permeable membranes for separation processes or apparatus characterised by the material; Manufacturing processes specially adapted therefor
    • B01D71/06Organic material
    • B01D71/58Other polymers having nitrogen in the main chain, with or without oxygen or carbon only
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10TTECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00Chemistry: analytical and immunological testing
    • Y10T436/14Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/145555Hetero-N

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Zoology (AREA)
  • Nanotechnology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Manufacturing & Machinery (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Compositions Of Macromolecular Compounds (AREA)
AU2013336430A 2012-10-26 2013-10-23 Droplet interfaces Active AU2013336430B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261718982P 2012-10-26 2012-10-26
US61/718,982 2012-10-26
PCT/GB2013/052767 WO2014064444A1 (en) 2012-10-26 2013-10-23 Droplet interfaces

Publications (2)

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AU2013336430A1 AU2013336430A1 (en) 2015-05-14
AU2013336430B2 true AU2013336430B2 (en) 2018-02-15

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US (1) US9823235B2 (OSRAM)
EP (1) EP2911771B1 (OSRAM)
JP (1) JP6375301B2 (OSRAM)
CN (1) CN104936682B (OSRAM)
AU (1) AU2013336430B2 (OSRAM)
CA (1) CA2889664C (OSRAM)
WO (1) WO2014064444A1 (OSRAM)

Families Citing this family (140)

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Publication number Priority date Publication date Assignee Title
AU2008217578A1 (en) 2007-02-20 2008-08-28 Oxford Nanopore Technologies Limited Lipid bilayer sensor system
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
JP5843614B2 (ja) 2009-01-30 2016-01-13 オックスフォード ナノポア テクノロジーズ リミテッド 膜貫通配列決定における核酸構築物のためのアダプター
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
WO2012164270A1 (en) 2011-05-27 2012-12-06 Oxford Nanopore Technologies Limited Coupling method
IN2014DN00221A (OSRAM) 2011-07-25 2015-06-05 Oxford Nanopore Tech Ltd
WO2013041878A1 (en) 2011-09-23 2013-03-28 Oxford Nanopore Technologies Limited Analysis of a polymer comprising polymer units
EP2987870B1 (en) 2011-10-21 2020-02-19 Oxford Nanopore Technologies Limited Method of characterizing a target polynucleotide using a transmembrane pore and molecular motor
GB201119032D0 (en) 2011-11-03 2011-12-14 Isis Innovation Multisomes: encapsulated droplet networks
GB201202519D0 (en) 2012-02-13 2012-03-28 Oxford Nanopore Tech Ltd Apparatus for supporting an array of layers of amphiphilic molecules and method of forming an array of layers of amphiphilic molecules
WO2013121224A1 (en) 2012-02-16 2013-08-22 Oxford Nanopore Technologies Limited Analysis of measurements of a polymer
KR102083695B1 (ko) 2012-04-10 2020-03-02 옥스포드 나노포어 테크놀로지즈 리미티드 돌연변이체 리세닌 기공
WO2014013259A1 (en) 2012-07-19 2014-01-23 Oxford Nanopore Technologies Limited Ssb method
US10808231B2 (en) 2012-07-19 2020-10-20 Oxford Nanopore Technologies Limited Modified helicases
GB201219196D0 (en) 2012-10-25 2012-12-12 Isis Innovation Droplet assembly method
GB201219201D0 (en) 2012-10-25 2012-12-12 Isis Innovation Hydrogel network
GB201313121D0 (en) 2013-07-23 2013-09-04 Oxford Nanopore Tech Ltd Array of volumes of polar medium
US11213797B2 (en) * 2012-12-07 2022-01-04 Oxford University Innovation Limited Droplet assembly by 3D printing
GB201222928D0 (en) 2012-12-19 2013-01-30 Oxford Nanopore Tech Ltd Analysis of a polynucleotide
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
EP2964779B1 (en) 2013-03-08 2018-08-29 Oxford Nanopore Technologies Limited Use of spacer elements in a nucleic acid to control movement of a helicase
GB201313477D0 (en) 2013-07-29 2013-09-11 Univ Leuven Kath Nanopore biosensors for detection of proteins and nucleic acids
CN118086476A (zh) 2013-10-18 2024-05-28 牛津纳米孔科技公开有限公司 经修饰的酶
CN111534504B (zh) 2014-01-22 2024-06-21 牛津纳米孔科技公开有限公司 将一个或多个多核苷酸结合蛋白连接到靶多核苷酸的方法
GB201406155D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
CN106460061B (zh) 2014-04-04 2020-03-06 牛津纳米孔技术公司 在双链核酸分子的两端使用纳米孔和锚分子来表征所述双链核酸的方法
GB201417712D0 (en) 2014-10-07 2014-11-19 Oxford Nanopore Tech Ltd Method
CN106459159B (zh) 2014-05-02 2021-11-30 牛津纳米孔技术公司 突变孔
EP3831956A1 (en) 2014-07-14 2021-06-09 Oxford University Innovation Limited Measurement of analytes with membrane channel molecules, and bilayer arrays
US10400014B2 (en) 2014-09-01 2019-09-03 Oxford Nanopore Technologies Ltd. Mutant CsgG pores
WO2016055778A1 (en) 2014-10-07 2016-04-14 Oxford Nanopore Technologies Limited Mutant pores
GB201418159D0 (en) 2014-10-14 2014-11-26 Oxford Nanopore Tech Ltd Method
EP3971300B1 (en) 2014-10-16 2024-06-05 Oxford Nanopore Technologies plc Sorting of polymers
GB201418469D0 (en) 2014-10-17 2014-12-03 Oxford Nanopore Tech Ltd Method
GB201418512D0 (en) 2014-10-17 2014-12-03 Oxford Nanopore Tech Ltd Electrical device with detachable components
GB201502810D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Method
GB201502809D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Mutant pore
CN107735686B (zh) 2015-04-14 2021-06-11 鲁汶天主教大学 具有内部蛋白质衔接子的纳米孔
CN107923912B (zh) * 2015-07-02 2023-12-01 马萨诸塞大学 膜和液滴界面双层系统及方法
CN109072295B (zh) 2015-12-08 2022-05-31 鲁汶大学研究与发展中心 修饰的纳米孔,包含其的组合物及其应用
KR20180117179A (ko) * 2016-03-01 2018-10-26 옥스포드 유니버시티 이노베이션 리미티드 셀룰러화 스캐폴드의 3d 인쇄
GB201603591D0 (en) 2016-03-01 2016-04-13 Isis Innovation Improved promotors and compositions
US10975428B2 (en) 2016-03-02 2021-04-13 Oxford Nanopore Technologies Ltd. Mutant pore
CN118326018B (zh) 2016-04-06 2025-11-07 牛津纳米孔科技公开有限公司 突变体孔
GB201609220D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
EP4063521B1 (en) 2016-05-25 2024-02-21 Oxford Nanopore Technologies PLC Method of nanopore sequencing
GB201609221D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
GB201611770D0 (en) 2016-07-06 2016-08-17 Oxford Nanopore Tech Microfluidic device
GB201615741D0 (en) * 2016-09-15 2016-11-02 Univ College Cardiff Consultants Ltd Artificial cells
GB201616590D0 (en) 2016-09-29 2016-11-16 Oxford Nanopore Technologies Limited Method
CN106474946A (zh) * 2016-10-17 2017-03-08 东华大学 一种具有亲水抗污性能的聚合物膜的制备方法
GB201619930D0 (en) 2016-11-24 2017-01-11 Oxford Nanopore Tech Apparatus and methods for controlling insertion of a membrane channel into a membrane
US10876060B2 (en) * 2016-11-29 2020-12-29 Kamterter Products, Llc Droplet for fuels
GB201620450D0 (en) 2016-12-01 2017-01-18 Oxford Nanopore Tech Ltd Method
GB2559117B (en) 2017-01-19 2019-11-27 Oxford Nanopore Tech Ltd Double stranded polynucleotide synthesis method, kit and system
CN119530363A (zh) 2017-02-10 2025-02-28 牛津纳米孔科技公开有限公司 修饰的纳米孔、包含其的组合物及其用途
GB201707140D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Method
GB201707122D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Pore
GB201707138D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Machine learning analysis of nanopore measurements
CN117106037B (zh) 2017-06-30 2025-07-25 弗拉芒区生物技术研究所 新颖蛋白孔
GB2568895B (en) 2017-11-29 2021-10-27 Oxford Nanopore Tech Ltd Microfluidic device
GB2569630B (en) 2017-12-21 2022-10-12 Sharp Life Science Eu Ltd Droplet Interfaces in Electro-wetting Devices
US11293911B2 (en) 2017-12-28 2022-04-05 Korea Advanced Institute Of Science And Technology Multi-phase liquid composition, device for measuring permeability of drugs, and method for measuring permeability of drugs
GB201801768D0 (en) 2018-02-02 2018-03-21 Oxford Nanopore Tech Ltd Synthesis method
GB201807793D0 (en) 2018-05-14 2018-06-27 Oxford Nanopore Tech Ltd Method
GB2574197B (en) 2018-05-23 2022-01-05 Oxford Nanopore Tech Ltd Double stranded polynucleotide synthesis method and system.
GB2574048B (en) 2018-05-24 2021-06-16 Oxford Nanopore Tech Ltd Nanopore sensor component with electrostatic discharge protection
US12269037B2 (en) * 2018-05-24 2025-04-08 Oxford Nanopore Technologies Plc Droplet interfaces in electro-wetting devices
GB201809323D0 (en) 2018-06-06 2018-07-25 Oxford Nanopore Tech Ltd Method
GB201811811D0 (en) 2018-07-19 2018-09-05 Oxford Nanopore Tech Ltd Method
GB201814369D0 (en) 2018-09-04 2018-10-17 Oxford Nanopore Tech Ltd Method for determining a polymersequence
JP2022502094A (ja) 2018-10-08 2022-01-11 アドルフ・メークル・インスティチュート、ユニバーシティー・オブ・フリブール 細孔サイズ、膜親和性、安定性、および抗菌活性を高めるための、細孔形成ペプチドのオリゴヌクレオチドベースの調整
GB201818216D0 (en) 2018-11-08 2018-12-26 Oxford Nanopore Tech Ltd Pore
CN113195736A (zh) 2018-11-08 2021-07-30 牛津纳米孔科技公司
GB201819378D0 (en) 2018-11-28 2019-01-09 Oxford Nanopore Tech Ltd Analysis of nanopore signal using a machine-learning technique
EP3887046A1 (en) 2018-11-28 2021-10-06 Oxford Nanopore Technologies Limited Sensing system and method of operation
JP2022511880A (ja) * 2018-12-11 2022-02-01 エフ.ホフマン-ラ ロシュ アーゲー 膜における自己制限性プロテイン細孔挿入のためのシステム及び方法
GB201821155D0 (en) 2018-12-21 2019-02-06 Oxford Nanopore Tech Ltd Method
AU2020239385A1 (en) 2019-03-12 2021-08-26 Oxford Nanopore Technologies Plc Nanopore sensing device and methods of operation and of forming it
GB2580988B (en) 2019-03-19 2022-04-13 Oxford Nanopore Tech Ltd Current measurement apparatus, molecular entity sensing apparatus, method of measuring a current, method of sensing a molecular entity
AU2020272997A1 (en) 2019-04-09 2021-10-21 Oxford Nanopore Technologies Plc Pore
GB201907244D0 (en) 2019-05-22 2019-07-03 Oxford Nanopore Tech Ltd Method
GB201907246D0 (en) 2019-05-22 2019-07-03 Oxford Nanopore Tech Ltd Method
GB201907243D0 (en) 2019-05-22 2019-07-03 Oxford Nanopore Tech Ltd Sensing interactions between molecular entities and nanapores
WO2020239066A1 (en) 2019-05-29 2020-12-03 Nanjing University Detection of analytes by nanopore without using electrodes
GB201915480D0 (en) 2019-10-25 2019-12-11 Oxford Nanopore Tech Ltd Improved nanopore sensing device, components and method of manufacture
GB201917060D0 (en) 2019-11-22 2020-01-08 Oxford Nanopore Tech Ltd Method
JP2023503514A (ja) 2019-12-02 2023-01-30 オックスフォード ナノポール テクノロジーズ ピーエルシー ナノポアを使用する標的ペプチドの特徴付けの方法
GB201917742D0 (en) 2019-12-04 2020-01-15 Oxford Nanopore Tech Ltd Method
GB202004944D0 (en) 2020-04-03 2020-05-20 King S College London Method
GB202016874D0 (en) 2020-10-23 2020-12-09 Oxford Nanopore Tech Ltd Nanopore support structure and manufacture thereof
US20230295712A1 (en) 2020-06-18 2023-09-21 Oxford Nanopore Technologies Plc A method of selectively characterising a polynucleotide using a detector
GB202009349D0 (en) 2020-06-18 2020-08-05 Oxford Nanopore Tech Ltd Method
US20240076729A9 (en) 2020-06-18 2024-03-07 Oxford Nanopore Technologies Plc Method
KR20230038566A (ko) 2020-07-17 2023-03-20 옥스포드 나노포어 테크놀로지즈 피엘씨 나노포어 감지 디바이스
US20230266268A1 (en) 2020-07-22 2023-08-24 Oxford Nanopore Technologies Inc. Solid state nanopore formation
WO2022051888A1 (zh) * 2020-09-08 2022-03-17 三诺生物传感股份有限公司 生物传感器的成膜组合物及其制备方法
GB202015993D0 (en) 2020-10-08 2020-11-25 Oxford Nanopore Tech Ltd Method
EP4341433A1 (en) 2021-05-19 2024-03-27 Oxford Nanopore Technologies PLC Methods for complement strand sequencing
GB202107192D0 (en) 2021-05-19 2021-06-30 Oxford Nanopore Tech Ltd Method
GB202107354D0 (en) 2021-05-24 2021-07-07 Oxford Nanopore Tech Ltd Method
EP4441744A1 (en) 2021-11-29 2024-10-09 Oxford Nanopore Technologies Ltd. Nanopore measurement signal analysis
CN116297721A (zh) * 2021-12-21 2023-06-23 成都齐碳科技有限公司 成膜方法、包含膜的系统及应用
GB202118908D0 (en) 2021-12-23 2022-02-09 Oxford Nanopore Tech Ltd Method
GB202118906D0 (en) 2021-12-23 2022-02-09 Oxford Nanopore Tech Ltd Method
GB202118939D0 (en) 2021-12-23 2022-02-09 Oxford Nanopore Tech Plc Pore
CN118414205A (zh) * 2021-12-31 2024-07-30 深圳华大生命科学研究院 一种磷脂/聚合物仿生复合配方膜及其制备方法和应用
GB202202716D0 (en) 2022-02-28 2022-04-13 Oxford Nanopore Tech Plc Apparatus and methods for controlling insertion of a membrane channel into a membrane
GB202205617D0 (en) 2022-04-14 2022-06-01 Oxford Nanopore Tech Plc Novel modified protein pores and enzymes
WO2023222657A1 (en) 2022-05-17 2023-11-23 Oxford Nanopore Technologies Plc Method and adaptors
GB202207267D0 (en) 2022-05-18 2022-06-29 Oxford Nanopore Tech Plc Calibration and profiling of a nanopore array device
CN115068336A (zh) * 2022-06-15 2022-09-20 厦门承葛生物科技有限公司 一种基于液滴微流控的肠道菌群包埋工艺
EP4569330A1 (en) 2022-08-09 2025-06-18 Oxford Nanopore Technologies PLC Novel pore monomers and pores
GB202211602D0 (en) 2022-08-09 2022-09-21 Oxford Nanopore Tech Plc Novel pore monomers and pores
GB202211607D0 (en) 2022-08-09 2022-09-21 Oxford Nanopore Tech Plc Novel pore monomers and pores
CA3262945A1 (en) 2022-08-09 2024-02-15 The Regents Of The University Of California DE NOVO PORES
WO2024055284A1 (zh) * 2022-09-16 2024-03-21 深圳华大生命科学研究院 一种离子交联的仿生膜、其制备方法及应用
GB202215442D0 (en) 2022-10-19 2022-11-30 Oxford Nanopore Tech Plc Analysis of a polymer
CN120303290A (zh) 2022-10-28 2025-07-11 牛津纳米孔科技公开有限公司 孔单体和孔
GB202216162D0 (en) 2022-10-31 2022-12-14 Oxford Nanopore Tech Plc Method
CN120202306A (zh) 2022-11-01 2025-06-24 牛津纳米孔科技公开有限公司 生化分析系统以及控制生化分析系统的方法
GB202216905D0 (en) 2022-11-11 2022-12-28 Oxford Nanopore Tech Plc Novel pore monomers and pores
GB202301095D0 (en) 2023-01-25 2023-03-08 Oxford Nanopore Tech Plc Calibration of a nanopore array device
CN117384486B (zh) * 2023-02-10 2025-06-27 北京普译生物科技有限公司 一种混合仿生膜及其制备方法和应用
GB202304324D0 (en) 2023-03-24 2023-05-10 Oxford Nanopore Tech Plc Method and kits
GB202305524D0 (en) 2023-04-14 2023-05-31 Oxford Nanopore Tech Plc Novel pore monomers and pores
WO2024227838A1 (en) * 2023-05-04 2024-11-07 F. Hoffmann-La Roche Ag Modified triblock copolymer compounds and methods of use thereof
GB202307141D0 (en) 2023-05-12 2023-06-28 Oxford Nanopore Tech Plc Modified helicases
GB202307486D0 (en) 2023-05-18 2023-07-05 Oxford Nanopore Tech Plc Method
CN119327288A (zh) * 2023-07-18 2025-01-21 国家能源投资集团有限责任公司 一种用于海水电解制氢的正渗透膜及其制备方法
GB202315935D0 (en) 2023-10-18 2023-11-29 Oxford Nanopore Tech Ltd Novel enzymes
GB202317028D0 (en) 2023-11-06 2023-12-20 Oxford Nanopore Tech Ltd Method
GB202405960D0 (en) 2024-04-29 2024-06-12 Oxford Nanopore Tech Plc Photo-active conjugates and their uses
GB202407100D0 (en) 2024-05-17 2024-07-03 Oxford Nanopore Tech Plc A flow cell and a method
GB202407099D0 (en) 2024-05-17 2024-07-03 Oxford Nanopore Tech Plc A flow cell and a method
GB202407055D0 (en) 2024-05-17 2024-07-03 Oxford Nanopore Tech Plc Enhanced polynucleotide sequencing
GB202407044D0 (en) 2024-05-17 2024-07-03 Oxford Nanopore Tech Plc Polymer analysis using transformer neural network
GB202407228D0 (en) 2024-05-21 2024-07-03 Oxford Nanopore Tech Plc Method
GB202407247D0 (en) 2024-05-21 2024-07-03 Oxford Nanopore Tech Plc Method

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001032146A2 (en) * 1999-11-05 2001-05-10 Biocure, Inc. Amphiphilic polymeric vesicles
WO2001088025A1 (en) * 2000-05-16 2001-11-22 Biocure, Inc. Membranes formed from amphiphilic copolymers
WO2009024775A1 (en) * 2007-08-21 2009-02-26 Isis Innovation Limited Bilayers

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5807944A (en) * 1996-06-27 1998-09-15 Ciba Vision Corporation Amphiphilic, segmented copolymer of controlled morphology and ophthalmic devices including contact lenses made therefrom
US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
MXPA05003744A (es) * 2002-10-11 2005-09-20 Idea Ag Agregado de deformabilidad aumentada, que comprende al menos tres anfipatos, para un transporte mejorado a traves de barreras semi-permeables y para la aplicacion de farmacos no invasivos en vivo, en especial a traves de la piel.
WO2005124888A1 (en) 2004-06-08 2005-12-29 President And Fellows Of Harvard College Suspended carbon nanotube field effect transistor
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
JP5114702B2 (ja) * 2005-07-29 2013-01-09 国立大学法人 東京大学 両親媒性単分子膜の接触による二分子膜の形成方法およびその装置
AT502713B1 (de) * 2005-10-19 2008-08-15 Univ Wien Bodenkultur Verfahren zur herstellung von lipid-membranen
GB0614835D0 (en) * 2006-07-26 2006-09-06 Isis Innovation Formation of bilayers of amphipathic molecules
US8961898B2 (en) * 2007-03-30 2015-02-24 Tokyo Institute Of Technology Method for producing bilayer membrane and planar bilayer membrane
US8101274B2 (en) * 2007-06-11 2012-01-24 Spedden Richard H Solid state membranes with surface-embedded glycosylated amphiphilic molecules and micelles formed therefrom
BRPI0706005A2 (pt) 2007-07-24 2009-03-17 Orbisat Da Amazonia Ind E Aero método e sistema de difusão de serviços digitais com recepção particularizada por região
US8293339B2 (en) * 2007-09-17 2012-10-23 Sri International, Inc. Droplet bilayers
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
EP2307540B1 (en) 2008-07-07 2017-04-19 Oxford Nanopore Technologies Limited Enzyme-pore constructs
WO2010004273A1 (en) 2008-07-07 2010-01-14 Oxford Nanopore Technologies Limited Base-detecting pore
KR20110125226A (ko) 2009-01-30 2011-11-18 옥스포드 나노포어 테크놀로지즈 리미티드 혼성화 링커
EP2422198B1 (en) 2009-04-20 2013-09-25 Oxford Nanopore Technologies Limited Lipid bilayer sensor array
EP2422194B1 (en) * 2009-04-20 2014-12-17 Agency For Science, Technology And Research Vesicular system and uses thereof
GB0913823D0 (en) * 2009-08-07 2009-09-16 Isis Innovation Bilayers
CN102741430B (zh) 2009-12-01 2016-07-13 牛津楠路珀尔科技有限公司 生化分析仪器、用于进行生化分析的第一模块以及相关方法
GB201313121D0 (en) * 2013-07-23 2013-09-04 Oxford Nanopore Tech Ltd Array of volumes of polar medium

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001032146A2 (en) * 1999-11-05 2001-05-10 Biocure, Inc. Amphiphilic polymeric vesicles
WO2001088025A1 (en) * 2000-05-16 2001-11-22 Biocure, Inc. Membranes formed from amphiphilic copolymers
WO2009024775A1 (en) * 2007-08-21 2009-02-26 Isis Innovation Limited Bilayers

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