AU2012280941B2 - Stacked herbicide tolerance event 8264.42.32.1, related transgenic soybean lines, and detection thereof - Google Patents
Stacked herbicide tolerance event 8264.42.32.1, related transgenic soybean lines, and detection thereof Download PDFInfo
- Publication number
- AU2012280941B2 AU2012280941B2 AU2012280941A AU2012280941A AU2012280941B2 AU 2012280941 B2 AU2012280941 B2 AU 2012280941B2 AU 2012280941 A AU2012280941 A AU 2012280941A AU 2012280941 A AU2012280941 A AU 2012280941A AU 2012280941 B2 AU2012280941 B2 AU 2012280941B2
- Authority
- AU
- Australia
- Prior art keywords
- seq
- soybean
- event
- plant
- dna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
- 244000068988 Glycine max Species 0.000 title claims abstract description 348
- 235000010469 Glycine max Nutrition 0.000 title claims abstract description 291
- 239000004009 herbicide Substances 0.000 title claims abstract description 127
- 230000002363 herbicidal effect Effects 0.000 title claims abstract description 69
- 230000009261 transgenic effect Effects 0.000 title claims abstract description 66
- 238000001514 detection method Methods 0.000 title abstract description 26
- 241000196324 Embryophyta Species 0.000 claims abstract description 220
- 238000000034 method Methods 0.000 claims abstract description 113
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 103
- 230000014509 gene expression Effects 0.000 claims abstract description 52
- 239000005562 Glyphosate Substances 0.000 claims abstract description 47
- XDDAORKBJWWYJS-UHFFFAOYSA-N glyphosate Chemical compound OC(=O)CNCP(O)(O)=O XDDAORKBJWWYJS-UHFFFAOYSA-N 0.000 claims abstract description 47
- 229940097068 glyphosate Drugs 0.000 claims abstract description 47
- IAJOBQBIJHVGMQ-UHFFFAOYSA-N 2-amino-4-[hydroxy(methyl)phosphoryl]butanoic acid Chemical compound CP(O)(=O)CCC(N)C(O)=O IAJOBQBIJHVGMQ-UHFFFAOYSA-N 0.000 claims abstract description 31
- 239000005561 Glufosinate Substances 0.000 claims abstract description 29
- 108020004414 DNA Proteins 0.000 claims description 175
- 239000000523 sample Substances 0.000 claims description 121
- 108091093088 Amplicon Proteins 0.000 claims description 52
- 108700019146 Transgenes Proteins 0.000 claims description 51
- 150000007523 nucleic acids Chemical class 0.000 claims description 46
- 239000012634 fragment Substances 0.000 claims description 43
- 238000003752 polymerase chain reaction Methods 0.000 claims description 42
- 108091033319 polynucleotide Proteins 0.000 claims description 41
- 102000040430 polynucleotide Human genes 0.000 claims description 41
- 239000002157 polynucleotide Substances 0.000 claims description 38
- 102000039446 nucleic acids Human genes 0.000 claims description 36
- 108020004707 nucleic acids Proteins 0.000 claims description 36
- 239000005631 2,4-Dichlorophenoxyacetic acid Substances 0.000 claims description 33
- 210000004027 cell Anatomy 0.000 claims description 25
- 125000003729 nucleotide group Chemical group 0.000 claims description 18
- 239000002773 nucleotide Substances 0.000 claims description 17
- 238000009395 breeding Methods 0.000 claims description 16
- 210000000349 chromosome Anatomy 0.000 claims description 16
- 230000000295 complement effect Effects 0.000 claims description 15
- 230000001488 breeding effect Effects 0.000 claims description 12
- 238000002866 fluorescence resonance energy transfer Methods 0.000 claims description 10
- 241001233957 eudicotyledons Species 0.000 claims description 9
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims description 7
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims description 7
- 238000013518 transcription Methods 0.000 claims description 7
- 230000035897 transcription Effects 0.000 claims description 7
- LLWADFLAOKUBDR-UHFFFAOYSA-N 2-methyl-4-chlorophenoxybutyric acid Chemical compound CC1=CC(Cl)=CC=C1OCCCC(O)=O LLWADFLAOKUBDR-UHFFFAOYSA-N 0.000 claims description 6
- 239000005575 MCPB Substances 0.000 claims description 6
- 101150039283 MCPB gene Proteins 0.000 claims description 6
- 238000004519 manufacturing process Methods 0.000 claims description 6
- 239000002794 2,4-DB Substances 0.000 claims description 5
- 239000005504 Dicamba Substances 0.000 claims description 5
- IWEDIXLBFLAXBO-UHFFFAOYSA-N dicamba Chemical group COC1=C(Cl)C=CC(Cl)=C1C(O)=O IWEDIXLBFLAXBO-UHFFFAOYSA-N 0.000 claims description 5
- 239000005574 MCPA Substances 0.000 claims description 4
- WHKUVVPPKQRRBV-UHFFFAOYSA-N Trasan Chemical compound CC1=CC(Cl)=CC=C1OCC(O)=O WHKUVVPPKQRRBV-UHFFFAOYSA-N 0.000 claims description 4
- IAJOBQBIJHVGMQ-BYPYZUCNSA-N glufosinate-P Chemical compound CP(O)(=O)CC[C@H](N)C(O)=O IAJOBQBIJHVGMQ-BYPYZUCNSA-N 0.000 claims description 4
- 230000002441 reversible effect Effects 0.000 claims description 4
- 235000012054 meals Nutrition 0.000 claims description 3
- GINJFDRNADDBIN-FXQIFTODSA-N bilanafos Chemical compound OC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@@H](N)CCP(C)(O)=O GINJFDRNADDBIN-FXQIFTODSA-N 0.000 claims description 2
- 238000007848 endpoint PCR Methods 0.000 claims 1
- 238000004458 analytical method Methods 0.000 abstract description 23
- 102000004169 proteins and genes Human genes 0.000 abstract description 23
- 238000003556 assay Methods 0.000 abstract description 14
- -1 aryloxyalkanoate Chemical compound 0.000 abstract description 6
- 238000003976 plant breeding Methods 0.000 abstract description 6
- 239000000463 material Substances 0.000 abstract description 4
- 238000002944 PCR assay Methods 0.000 abstract description 3
- 239000013615 primer Substances 0.000 description 119
- 238000009396 hybridization Methods 0.000 description 48
- 238000003780 insertion Methods 0.000 description 45
- 230000037431 insertion Effects 0.000 description 45
- 108091028043 Nucleic acid sequence Proteins 0.000 description 41
- 230000006378 damage Effects 0.000 description 34
- RBSXHDIPCIWOMG-UHFFFAOYSA-N 1-(4,6-dimethoxypyrimidin-2-yl)-3-(2-ethylsulfonylimidazo[1,2-a]pyridin-3-yl)sulfonylurea Chemical compound CCS(=O)(=O)C=1N=C2C=CC=CN2C=1S(=O)(=O)NC(=O)NC1=NC(OC)=CC(OC)=N1 RBSXHDIPCIWOMG-UHFFFAOYSA-N 0.000 description 33
- 208000027418 Wounds and injury Diseases 0.000 description 31
- 208000014674 injury Diseases 0.000 description 31
- 230000009418 agronomic effect Effects 0.000 description 30
- 238000003199 nucleic acid amplification method Methods 0.000 description 27
- 230000003321 amplification Effects 0.000 description 23
- 230000010354 integration Effects 0.000 description 22
- 239000013612 plasmid Substances 0.000 description 22
- 108010082527 phosphinothricin N-acetyltransferase Proteins 0.000 description 19
- 235000018102 proteins Nutrition 0.000 description 19
- 210000001519 tissue Anatomy 0.000 description 19
- 238000006243 chemical reaction Methods 0.000 description 18
- SHIBSTMRCDJXLN-UHFFFAOYSA-N Digoxigenin Natural products C1CC(C2C(C3(C)CCC(O)CC3CC2)CC2O)(O)C2(C)C1C1=CC(=O)OC1 SHIBSTMRCDJXLN-UHFFFAOYSA-N 0.000 description 17
- QONQRTHLHBTMGP-UHFFFAOYSA-N digitoxigenin Natural products CC12CCC(C3(CCC(O)CC3CC3)C)C3C11OC1CC2C1=CC(=O)OC1 QONQRTHLHBTMGP-UHFFFAOYSA-N 0.000 description 17
- SHIBSTMRCDJXLN-KCZCNTNESA-N digoxigenin Chemical compound C1([C@@H]2[C@@]3([C@@](CC2)(O)[C@H]2[C@@H]([C@@]4(C)CC[C@H](O)C[C@H]4CC2)C[C@H]3O)C)=CC(=O)OC1 SHIBSTMRCDJXLN-KCZCNTNESA-N 0.000 description 17
- 239000002987 primer (paints) Substances 0.000 description 17
- 239000003550 marker Substances 0.000 description 16
- 239000000203 mixture Substances 0.000 description 15
- 238000011282 treatment Methods 0.000 description 15
- 238000012790 confirmation Methods 0.000 description 14
- 230000002068 genetic effect Effects 0.000 description 14
- 238000002105 Southern blotting Methods 0.000 description 13
- 230000009466 transformation Effects 0.000 description 13
- 239000000047 product Substances 0.000 description 12
- 108091008146 restriction endonucleases Proteins 0.000 description 12
- 238000002474 experimental method Methods 0.000 description 11
- 239000012528 membrane Substances 0.000 description 11
- 230000006798 recombination Effects 0.000 description 11
- 238000005215 recombination Methods 0.000 description 11
- 239000000243 solution Substances 0.000 description 11
- 102000004190 Enzymes Human genes 0.000 description 10
- 108090000790 Enzymes Proteins 0.000 description 10
- 241000238631 Hexapoda Species 0.000 description 10
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 10
- 230000002759 chromosomal effect Effects 0.000 description 10
- 230000029087 digestion Effects 0.000 description 10
- 238000002744 homologous recombination Methods 0.000 description 10
- 108091092195 Intron Proteins 0.000 description 9
- 239000002253 acid Substances 0.000 description 9
- 239000000872 buffer Substances 0.000 description 9
- 230000006801 homologous recombination Effects 0.000 description 9
- 230000019612 pigmentation Effects 0.000 description 9
- 238000012163 sequencing technique Methods 0.000 description 9
- 239000003153 chemical reaction reagent Substances 0.000 description 8
- 230000012010 growth Effects 0.000 description 8
- 238000012360 testing method Methods 0.000 description 8
- 238000002965 ELISA Methods 0.000 description 7
- 206010028851 Necrosis Diseases 0.000 description 7
- 108091034117 Oligonucleotide Proteins 0.000 description 7
- 238000012408 PCR amplification Methods 0.000 description 7
- 208000006278 hypochromic anemia Diseases 0.000 description 7
- 230000035772 mutation Effects 0.000 description 7
- 230000017074 necrotic cell death Effects 0.000 description 7
- 230000005080 plant death Effects 0.000 description 7
- 150000003839 salts Chemical class 0.000 description 7
- 230000001568 sexual effect Effects 0.000 description 7
- 239000000126 substance Substances 0.000 description 7
- 241000589158 Agrobacterium Species 0.000 description 6
- 240000008042 Zea mays Species 0.000 description 6
- 230000008901 benefit Effects 0.000 description 6
- 238000012512 characterization method Methods 0.000 description 6
- 238000010367 cloning Methods 0.000 description 6
- 238000000926 separation method Methods 0.000 description 6
- 230000007704 transition Effects 0.000 description 6
- 230000000007 visual effect Effects 0.000 description 6
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Chemical group CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 description 5
- 102000053602 DNA Human genes 0.000 description 5
- 101150111720 EPSPS gene Proteins 0.000 description 5
- 238000010222 PCR analysis Methods 0.000 description 5
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 5
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 5
- 238000013461 design Methods 0.000 description 5
- 201000010099 disease Diseases 0.000 description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 5
- 230000002401 inhibitory effect Effects 0.000 description 5
- 238000007254 oxidation reaction Methods 0.000 description 5
- 101150113864 pat gene Proteins 0.000 description 5
- LCPDWSOZIOUXRV-UHFFFAOYSA-N phenoxyacetic acid Chemical compound OC(=O)COC1=CC=CC=C1 LCPDWSOZIOUXRV-UHFFFAOYSA-N 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 108090000765 processed proteins & peptides Proteins 0.000 description 5
- 230000000306 recurrent effect Effects 0.000 description 5
- 230000010076 replication Effects 0.000 description 5
- 239000011780 sodium chloride Substances 0.000 description 5
- 101150003560 trfA gene Proteins 0.000 description 5
- 238000005406 washing Methods 0.000 description 5
- 229930192334 Auxin Natural products 0.000 description 4
- 229920000742 Cotton Polymers 0.000 description 4
- 102000016680 Dioxygenases Human genes 0.000 description 4
- 108010028143 Dioxygenases Proteins 0.000 description 4
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 4
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 4
- 239000004677 Nylon Substances 0.000 description 4
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 4
- 108010017070 Zinc Finger Nucleases Proteins 0.000 description 4
- 235000001014 amino acid Nutrition 0.000 description 4
- 239000002363 auxin Substances 0.000 description 4
- 230000008859 change Effects 0.000 description 4
- 230000001276 controlling effect Effects 0.000 description 4
- 238000012217 deletion Methods 0.000 description 4
- 230000037430 deletion Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000010348 incorporation Methods 0.000 description 4
- 230000001965 increasing effect Effects 0.000 description 4
- 235000009973 maize Nutrition 0.000 description 4
- 229920001778 nylon Polymers 0.000 description 4
- 229920001184 polypeptide Polymers 0.000 description 4
- 102000004196 processed proteins & peptides Human genes 0.000 description 4
- UNFWWIHTNXNPBV-WXKVUWSESA-N spectinomycin Chemical compound O([C@@H]1[C@@H](NC)[C@@H](O)[C@H]([C@@H]([C@H]1O1)O)NC)[C@]2(O)[C@H]1O[C@H](C)CC2=O UNFWWIHTNXNPBV-WXKVUWSESA-N 0.000 description 4
- 229960000268 spectinomycin Drugs 0.000 description 4
- 238000012546 transfer Methods 0.000 description 4
- 239000013598 vector Substances 0.000 description 4
- 239000011534 wash buffer Substances 0.000 description 4
- UMPSXRYVXUPCOS-UHFFFAOYSA-N 2,3-dichlorophenol Chemical compound OC1=CC=CC(Cl)=C1Cl UMPSXRYVXUPCOS-UHFFFAOYSA-N 0.000 description 3
- 108020005345 3' Untranslated Regions Proteins 0.000 description 3
- CAAMSDWKXXPUJR-UHFFFAOYSA-N 3,5-dihydro-4H-imidazol-4-one Chemical compound O=C1CNC=N1 CAAMSDWKXXPUJR-UHFFFAOYSA-N 0.000 description 3
- 108020004705 Codon Proteins 0.000 description 3
- 108020001019 DNA Primers Proteins 0.000 description 3
- 230000004544 DNA amplification Effects 0.000 description 3
- 239000003155 DNA primer Substances 0.000 description 3
- 239000003298 DNA probe Substances 0.000 description 3
- 241001600125 Delftia acidovorans Species 0.000 description 3
- 101100313146 Dictyostelium discoideum tbpA gene Proteins 0.000 description 3
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 238000009015 Human TaqMan MicroRNA Assay kit Methods 0.000 description 3
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 3
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 3
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 3
- 102000018120 Recombinases Human genes 0.000 description 3
- 108010091086 Recombinases Proteins 0.000 description 3
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 238000000692 Student's t-test Methods 0.000 description 3
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 3
- 239000004473 Threonine Substances 0.000 description 3
- 108091023045 Untranslated Region Proteins 0.000 description 3
- 239000004480 active ingredient Substances 0.000 description 3
- 229940024606 amino acid Drugs 0.000 description 3
- 150000001413 amino acids Chemical group 0.000 description 3
- 230000000903 blocking effect Effects 0.000 description 3
- 244000038559 crop plants Species 0.000 description 3
- 238000007405 data analysis Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 229960000310 isoleucine Drugs 0.000 description 3
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 3
- 239000013642 negative control Substances 0.000 description 3
- 239000002853 nucleic acid probe Substances 0.000 description 3
- 238000009401 outcrossing Methods 0.000 description 3
- 231100000208 phytotoxic Toxicity 0.000 description 3
- 230000000885 phytotoxic effect Effects 0.000 description 3
- 229920002578 polythiourethane polymer Polymers 0.000 description 3
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 3
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 3
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 3
- 239000013641 positive control Substances 0.000 description 3
- 229960002429 proline Drugs 0.000 description 3
- 235000013930 proline Nutrition 0.000 description 3
- 238000010791 quenching Methods 0.000 description 3
- 230000000171 quenching effect Effects 0.000 description 3
- 238000001228 spectrum Methods 0.000 description 3
- 230000008685 targeting Effects 0.000 description 3
- 101150090317 tfdA gene Proteins 0.000 description 3
- IYLGZMTXKJYONK-ACLXAEORSA-N (12s,15r)-15-hydroxy-11,16-dioxo-15,20-dihydrosenecionan-12-yl acetate Chemical compound O1C(=O)[C@](CC)(O)C[C@@H](C)[C@](C)(OC(C)=O)C(=O)OCC2=CCN3[C@H]2[C@H]1CC3 IYLGZMTXKJYONK-ACLXAEORSA-N 0.000 description 2
- LUBJCRLGQSPQNN-UHFFFAOYSA-N 1-Phenylurea Chemical compound NC(=O)NC1=CC=CC=C1 LUBJCRLGQSPQNN-UHFFFAOYSA-N 0.000 description 2
- OVSKIKFHRZPJSS-UHFFFAOYSA-N 2,4-D Chemical compound OC(=O)COC1=CC=C(Cl)C=C1Cl OVSKIKFHRZPJSS-UHFFFAOYSA-N 0.000 description 2
- HXKWSTRRCHTUEC-UHFFFAOYSA-N 2,4-Dichlorophenoxyaceticacid Chemical compound OC(=O)C(Cl)OC1=CC=C(Cl)C=C1 HXKWSTRRCHTUEC-UHFFFAOYSA-N 0.000 description 2
- TVSPPYGAFOVROT-UHFFFAOYSA-N 2-phenoxybutanoic acid Chemical compound CCC(C(O)=O)OC1=CC=CC=C1 TVSPPYGAFOVROT-UHFFFAOYSA-N 0.000 description 2
- 108020005065 3' Flanking Region Proteins 0.000 description 2
- UPMXNNIRAGDFEH-UHFFFAOYSA-N 3,5-dibromo-4-hydroxybenzonitrile Chemical compound OC1=C(Br)C=C(C#N)C=C1Br UPMXNNIRAGDFEH-UHFFFAOYSA-N 0.000 description 2
- 108010020183 3-phosphoshikimate 1-carboxyvinyltransferase Proteins 0.000 description 2
- 108020005029 5' Flanking Region Proteins 0.000 description 2
- 108010000700 Acetolactate synthase Proteins 0.000 description 2
- 241000589155 Agrobacterium tumefaciens Species 0.000 description 2
- 102000000383 Alpha-Ketoglutarate-Dependent Dioxygenase FTO Human genes 0.000 description 2
- 108010016119 Alpha-Ketoglutarate-Dependent Dioxygenase FTO Proteins 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- 239000005489 Bromoxynil Substances 0.000 description 2
- 241001515826 Cassava vein mosaic virus Species 0.000 description 2
- 108091026890 Coding region Proteins 0.000 description 2
- 108700010070 Codon Usage Proteins 0.000 description 2
- 208000035240 Disease Resistance Diseases 0.000 description 2
- 238000008157 ELISA kit Methods 0.000 description 2
- 108010042407 Endonucleases Proteins 0.000 description 2
- 102000004533 Endonucleases Human genes 0.000 description 2
- 239000005558 Fluroxypyr Substances 0.000 description 2
- 239000004471 Glycine Substances 0.000 description 2
- 108010033040 Histones Proteins 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- 241000209510 Liliopsida Species 0.000 description 2
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 2
- 108700026244 Open Reading Frames Proteins 0.000 description 2
- 108091093037 Peptide nucleic acid Proteins 0.000 description 2
- ZYFVNVRFVHJEIU-UHFFFAOYSA-N PicoGreen Chemical compound CN(C)CCCN(CCCN(C)C)C1=CC(=CC2=[N+](C3=CC=CC=C3S2)C)C2=CC=CC=C2N1C1=CC=CC=C1 ZYFVNVRFVHJEIU-UHFFFAOYSA-N 0.000 description 2
- 229920001213 Polysorbate 20 Polymers 0.000 description 2
- 108020001991 Protoporphyrinogen Oxidase Proteins 0.000 description 2
- 102100029028 Protoporphyrinogen oxidase Human genes 0.000 description 2
- 108020004511 Recombinant DNA Proteins 0.000 description 2
- 101710088839 Replication initiation protein Proteins 0.000 description 2
- 241000187191 Streptomyces viridochromogenes Species 0.000 description 2
- 239000005627 Triclopyr Substances 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- 239000011543 agarose gel Substances 0.000 description 2
- 238000000246 agarose gel electrophoresis Methods 0.000 description 2
- 125000003295 alanine group Chemical group N[C@@H](C)C(=O)* 0.000 description 2
- 230000004075 alteration Effects 0.000 description 2
- 230000010165 autogamy Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 229940098773 bovine serum albumin Drugs 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 238000003776 cleavage reaction Methods 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 230000002939 deleterious effect Effects 0.000 description 2
- 239000003599 detergent Substances 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- UQLDLKMNUJERMK-UHFFFAOYSA-L di(octadecanoyloxy)lead Chemical compound [Pb+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O UQLDLKMNUJERMK-UHFFFAOYSA-L 0.000 description 2
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 2
- 238000001976 enzyme digestion Methods 0.000 description 2
- 230000036253 epinasty Effects 0.000 description 2
- 150000002148 esters Chemical group 0.000 description 2
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 2
- 229960005542 ethidium bromide Drugs 0.000 description 2
- 238000010195 expression analysis Methods 0.000 description 2
- MEFQWPUMEMWTJP-UHFFFAOYSA-N fluroxypyr Chemical compound NC1=C(Cl)C(F)=NC(OCC(O)=O)=C1Cl MEFQWPUMEMWTJP-UHFFFAOYSA-N 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- 239000003112 inhibitor Substances 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 229910001629 magnesium chloride Inorganic materials 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000001404 mediated effect Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000000877 morphologic effect Effects 0.000 description 2
- 238000005648 named reaction Methods 0.000 description 2
- 238000007899 nucleic acid hybridization Methods 0.000 description 2
- 239000002751 oligonucleotide probe Substances 0.000 description 2
- 230000036961 partial effect Effects 0.000 description 2
- 125000000951 phenoxy group Chemical group [H]C1=C([H])C([H])=C(O*)C([H])=C1[H] 0.000 description 2
- 239000002953 phosphate buffered saline Substances 0.000 description 2
- 108010001545 phytoene dehydrogenase Proteins 0.000 description 2
- 230000010287 polarization Effects 0.000 description 2
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 2
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 2
- 230000002285 radioactive effect Effects 0.000 description 2
- 238000003753 real-time PCR Methods 0.000 description 2
- IYLGZMTXKJYONK-UHFFFAOYSA-N ruwenine Natural products O1C(=O)C(CC)(O)CC(C)C(C)(OC(C)=O)C(=O)OCC2=CCN3C2C1CC3 IYLGZMTXKJYONK-UHFFFAOYSA-N 0.000 description 2
- 230000007017 scission Effects 0.000 description 2
- 238000005204 segregation Methods 0.000 description 2
- 239000001509 sodium citrate Substances 0.000 description 2
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 244000000000 soil microbiome Species 0.000 description 2
- 230000010473 stable expression Effects 0.000 description 2
- 238000012409 standard PCR amplification Methods 0.000 description 2
- 238000010561 standard procedure Methods 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 230000014616 translation Effects 0.000 description 2
- REEQLXCGVXDJSQ-UHFFFAOYSA-N trichlopyr Chemical compound OC(=O)COC1=NC(Cl)=C(Cl)C=C1Cl REEQLXCGVXDJSQ-UHFFFAOYSA-N 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- 108010053957 2,4-dichlorophenoxyacetate-alpha-ketoglutarate dioxygenase Proteins 0.000 description 1
- IUQJDHJVPLLKFL-UHFFFAOYSA-N 2-(2,4-dichlorophenoxy)acetate;dimethylazanium Chemical compound CNC.OC(=O)COC1=CC=C(Cl)C=C1Cl IUQJDHJVPLLKFL-UHFFFAOYSA-N 0.000 description 1
- OVSKIKFHRZPJSS-DOMIDYPGSA-N 2-(2,4-dichlorophenoxy)acetic acid Chemical compound OC(=O)[14CH2]OC1=CC=C(Cl)C=C1Cl OVSKIKFHRZPJSS-DOMIDYPGSA-N 0.000 description 1
- KPGXRSRHYNQIFN-UHFFFAOYSA-N 2-oxoglutaric acid Chemical group OC(=O)CCC(=O)C(O)=O KPGXRSRHYNQIFN-UHFFFAOYSA-N 0.000 description 1
- 240000006995 Abutilon theophrasti Species 0.000 description 1
- RZVAJINKPMORJF-UHFFFAOYSA-N Acetaminophen Chemical compound CC(=O)NC1=CC=C(O)C=C1 RZVAJINKPMORJF-UHFFFAOYSA-N 0.000 description 1
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
- 241000219318 Amaranthus Species 0.000 description 1
- 241001542006 Amaranthus palmeri Species 0.000 description 1
- 241000482638 Amaranthus tuberculatus Species 0.000 description 1
- 244000036975 Ambrosia artemisiifolia Species 0.000 description 1
- 235000003133 Ambrosia artemisiifolia Nutrition 0.000 description 1
- 241000208841 Ambrosia trifida Species 0.000 description 1
- 108010039224 Amidophosphoribosyltransferase Proteins 0.000 description 1
- 108010007730 Apyrase Proteins 0.000 description 1
- 102000007347 Apyrase Human genes 0.000 description 1
- 101500006437 Arabidopsis thaliana Ubiquitin Proteins 0.000 description 1
- 241001167018 Aroa Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 244000277285 Cassia obtusifolia Species 0.000 description 1
- 235000006719 Cassia obtusifolia Nutrition 0.000 description 1
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 description 1
- 240000006122 Chenopodium album Species 0.000 description 1
- 235000009344 Chenopodium album Nutrition 0.000 description 1
- 108010077544 Chromatin Proteins 0.000 description 1
- 240000001579 Cirsium arvense Species 0.000 description 1
- 235000005918 Cirsium arvense Nutrition 0.000 description 1
- 244000074881 Conyza canadensis Species 0.000 description 1
- 235000004385 Conyza canadensis Nutrition 0.000 description 1
- 244000289527 Cordyline terminalis Species 0.000 description 1
- 235000009091 Cordyline terminalis Nutrition 0.000 description 1
- 241000252867 Cupriavidus metallidurans Species 0.000 description 1
- IGXWBGJHJZYPQS-SSDOTTSWSA-N D-Luciferin Chemical compound OC(=O)[C@H]1CSC(C=2SC3=CC=C(O)C=C3N=2)=N1 IGXWBGJHJZYPQS-SSDOTTSWSA-N 0.000 description 1
- 238000001712 DNA sequencing Methods 0.000 description 1
- CYCGRDQQIOGCKX-UHFFFAOYSA-N Dehydro-luciferin Natural products OC(=O)C1=CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 CYCGRDQQIOGCKX-UHFFFAOYSA-N 0.000 description 1
- 101100536354 Drosophila melanogaster tant gene Proteins 0.000 description 1
- 241000702189 Escherichia virus Mu Species 0.000 description 1
- BJGNCJDXODQBOB-UHFFFAOYSA-N Fivefly Luciferin Natural products OC(=O)C1CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 BJGNCJDXODQBOB-UHFFFAOYSA-N 0.000 description 1
- 241000219146 Gossypium Species 0.000 description 1
- 244000020551 Helianthus annuus Species 0.000 description 1
- 235000003222 Helianthus annuus Nutrition 0.000 description 1
- 108010034791 Heterochromatin Proteins 0.000 description 1
- 101000829958 Homo sapiens N-acetyllactosaminide beta-1,6-N-acetylglucosaminyl-transferase Proteins 0.000 description 1
- XVOKUMIPKHGGTN-UHFFFAOYSA-N Imazethapyr Chemical compound OC(=O)C1=CC(CC)=CN=C1C1=NC(C)(C(C)C)C(=O)N1 XVOKUMIPKHGGTN-UHFFFAOYSA-N 0.000 description 1
- 229930010555 Inosine Natural products 0.000 description 1
- UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 description 1
- 102000012330 Integrases Human genes 0.000 description 1
- 108010061833 Integrases Proteins 0.000 description 1
- 235000021506 Ipomoea Nutrition 0.000 description 1
- 241000207783 Ipomoea Species 0.000 description 1
- 108060001084 Luciferase Proteins 0.000 description 1
- 239000005089 Luciferase Substances 0.000 description 1
- DDWFXDSYGUXRAY-UHFFFAOYSA-N Luciferin Natural products CCc1c(C)c(CC2NC(=O)C(=C2C=C)C)[nH]c1Cc3[nH]c4C(=C5/NC(CC(=O)O)C(C)C5CC(=O)O)CC(=O)c4c3C DDWFXDSYGUXRAY-UHFFFAOYSA-N 0.000 description 1
- 101100328463 Mus musculus Cmya5 gene Proteins 0.000 description 1
- 102100023315 N-acetyllactosaminide beta-1,6-N-acetylglucosaminyl-transferase Human genes 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 102000004316 Oxidoreductases Human genes 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 102000001406 Perilipin Human genes 0.000 description 1
- 108060006002 Perilipin Proteins 0.000 description 1
- 241000978467 Persicaria pensylvanica Species 0.000 description 1
- 108010081996 Photosystem I Protein Complex Proteins 0.000 description 1
- 108010060806 Photosystem II Protein Complex Proteins 0.000 description 1
- 239000004952 Polyamide Substances 0.000 description 1
- 244000110797 Polygonum persicaria Species 0.000 description 1
- 235000004442 Polygonum persicaria Nutrition 0.000 description 1
- OFOBLEOULBTSOW-UHFFFAOYSA-N Propanedioic acid Natural products OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 description 1
- 108020004518 RNA Probes Proteins 0.000 description 1
- 239000003391 RNA probe Substances 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 102000004523 Sulfate Adenylyltransferase Human genes 0.000 description 1
- 108010022348 Sulfate adenylyltransferase Proteins 0.000 description 1
- 229940100389 Sulfonylurea Drugs 0.000 description 1
- 239000008049 TAE buffer Substances 0.000 description 1
- 108010006785 Taq Polymerase Proteins 0.000 description 1
- 240000001949 Taraxacum officinale Species 0.000 description 1
- 235000006754 Taraxacum officinale Nutrition 0.000 description 1
- 235000005187 Taraxacum officinale ssp. officinale Nutrition 0.000 description 1
- 108700029229 Transcriptional Regulatory Elements Proteins 0.000 description 1
- 101150077913 VIP3 gene Proteins 0.000 description 1
- 108010059993 Vancomycin Proteins 0.000 description 1
- 244000067505 Xanthium strumarium Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 235000007244 Zea mays Nutrition 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 241001148683 Zostera marina Species 0.000 description 1
- 241000235033 Zygosaccharomyces rouxii Species 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- 125000000218 acetic acid group Chemical group C(C)(=O)* 0.000 description 1
- HGEVZDLYZYVYHD-UHFFFAOYSA-N acetic acid;2-amino-2-(hydroxymethyl)propane-1,3-diol;2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acid Chemical compound CC(O)=O.OCC(N)(CO)CO.OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O HGEVZDLYZYVYHD-UHFFFAOYSA-N 0.000 description 1
- 230000021736 acetylation Effects 0.000 description 1
- 238000006640 acetylation reaction Methods 0.000 description 1
- 108020002494 acetyltransferase Proteins 0.000 description 1
- 102000005421 acetyltransferase Human genes 0.000 description 1
- 208000005652 acute fatty liver of pregnancy Diseases 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- IRLPACMLTUPBCL-FCIPNVEPSA-N adenosine-5'-phosphosulfate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@@H](CO[P@](O)(=O)OS(O)(=O)=O)[C@H](O)[C@H]1O IRLPACMLTUPBCL-FCIPNVEPSA-N 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 125000000539 amino acid group Chemical group 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 238000010256 biochemical assay Methods 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000033558 biomineral tissue development Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
- 239000004327 boric acid Substances 0.000 description 1
- UDSAIICHUKSCKT-UHFFFAOYSA-N bromophenol blue Chemical compound C1=C(Br)C(O)=C(Br)C=C1C1(C=2C=C(Br)C(O)=C(Br)C=2)C2=CC=CC=C2S(=O)(=O)O1 UDSAIICHUKSCKT-UHFFFAOYSA-N 0.000 description 1
- 229960004261 cefotaxime Drugs 0.000 description 1
- AZZMGZXNTDTSME-JUZDKLSSSA-M cefotaxime sodium Chemical compound [Na+].N([C@@H]1C(N2C(=C(COC(C)=O)CS[C@@H]21)C([O-])=O)=O)C(=O)\C(=N/OC)C1=CSC(N)=N1 AZZMGZXNTDTSME-JUZDKLSSSA-M 0.000 description 1
- 238000012832 cell culture technique Methods 0.000 description 1
- 210000003855 cell nucleus Anatomy 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000005081 chemiluminescent agent Substances 0.000 description 1
- 210000003483 chromatin Anatomy 0.000 description 1
- 230000004186 co-expression Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000004590 computer program Methods 0.000 description 1
- 239000013068 control sample Substances 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 238000009795 derivation Methods 0.000 description 1
- 238000001784 detoxification Methods 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 239000000539 dimer Substances 0.000 description 1
- 238000003414 dioxygenase reaction Methods 0.000 description 1
- UKWLRLAKGMZXJC-QIECWBMSSA-L disodium;[4-chloro-3-[(3r,5s)-1-chloro-3'-methoxyspiro[adamantane-4,4'-dioxetane]-3'-yl]phenyl] phosphate Chemical compound [Na+].[Na+].O1OC2([C@@H]3CC4C[C@H]2CC(Cl)(C4)C3)C1(OC)C1=CC(OP([O-])([O-])=O)=CC=C1Cl UKWLRLAKGMZXJC-QIECWBMSSA-L 0.000 description 1
- 230000003828 downregulation Effects 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 238000009313 farming Methods 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000002875 fluorescence polarization Methods 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 230000030279 gene silencing Effects 0.000 description 1
- 238000012226 gene silencing method Methods 0.000 description 1
- 230000007614 genetic variation Effects 0.000 description 1
- 125000003630 glycyl group Chemical group [H]N([H])C([H])([H])C(*)=O 0.000 description 1
- 210000004458 heterochromatin Anatomy 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 229960003786 inosine Drugs 0.000 description 1
- 230000000749 insecticidal effect Effects 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 239000012160 loading buffer Substances 0.000 description 1
- 238000007403 mPCR Methods 0.000 description 1
- VZCYOOQTPOCHFL-UPHRSURJSA-N maleic acid Chemical compound OC(=O)\C=C/C(O)=O VZCYOOQTPOCHFL-UPHRSURJSA-N 0.000 description 1
- 239000011976 maleic acid Substances 0.000 description 1
- 230000037353 metabolic pathway Effects 0.000 description 1
- 230000011987 methylation Effects 0.000 description 1
- 238000007069 methylation reaction Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 239000003147 molecular marker Substances 0.000 description 1
- OKCDZCGCKMTKMK-UHFFFAOYSA-N n-phenyl-2h-triazolo[4,5-d]pyrimidine-5-sulfonamide Chemical compound N=1C=C2NN=NC2=NC=1S(=O)(=O)NC1=CC=CC=C1 OKCDZCGCKMTKMK-UHFFFAOYSA-N 0.000 description 1
- 230000003472 neutralizing effect Effects 0.000 description 1
- 210000004940 nucleus Anatomy 0.000 description 1
- 238000010422 painting Methods 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 229920002647 polyamide Polymers 0.000 description 1
- 125000001500 prolyl group Chemical group [H]N1C([H])(C(=O)[*])C([H])([H])C([H])([H])C1([H])[H] 0.000 description 1
- 238000001243 protein synthesis Methods 0.000 description 1
- 101150075980 psbA gene Proteins 0.000 description 1
- 238000012175 pyrosequencing Methods 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 239000011535 reaction buffer Substances 0.000 description 1
- 230000008707 rearrangement Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 1
- 238000009877 rendering Methods 0.000 description 1
- 230000008458 response to injury Effects 0.000 description 1
- 238000007894 restriction fragment length polymorphism technique Methods 0.000 description 1
- 229920002477 rna polymer Polymers 0.000 description 1
- 230000021749 root development Effects 0.000 description 1
- 239000012882 rooting medium Substances 0.000 description 1
- 230000010153 self-pollination Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- JXOHGGNKMLTUBP-HSUXUTPPSA-N shikimic acid Chemical compound O[C@@H]1CC(C(O)=O)=C[C@@H](O)[C@H]1O JXOHGGNKMLTUBP-HSUXUTPPSA-N 0.000 description 1
- JXOHGGNKMLTUBP-JKUQZMGJSA-N shikimic acid Natural products O[C@@H]1CC(C(O)=O)=C[C@H](O)[C@@H]1O JXOHGGNKMLTUBP-JKUQZMGJSA-N 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000011877 solvent mixture Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000011895 specific detection Methods 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- 230000002123 temporal effect Effects 0.000 description 1
- 125000000341 threoninyl group Chemical group [H]OC([H])(C([H])([H])[H])C([H])(N([H])[H])C(*)=O 0.000 description 1
- 229940027257 timentin Drugs 0.000 description 1
- 238000012090 tissue culture technique Methods 0.000 description 1
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 1
- 238000001890 transfection Methods 0.000 description 1
- 238000012250 transgenic expression Methods 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 230000003827 upregulation Effects 0.000 description 1
- MYPYJXKWCTUITO-LYRMYLQWSA-N vancomycin Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=C2C=C3C=C1OC1=CC=C(C=C1Cl)[C@@H](O)[C@H](C(N[C@@H](CC(N)=O)C(=O)N[C@H]3C(=O)N[C@H]1C(=O)N[C@H](C(N[C@@H](C3=CC(O)=CC(O)=C3C=3C(O)=CC=C1C=3)C(O)=O)=O)[C@H](O)C1=CC=C(C(=C1)Cl)O2)=O)NC(=O)[C@@H](CC(C)C)NC)[C@H]1C[C@](C)(N)[C@H](O)[C@H](C)O1 MYPYJXKWCTUITO-LYRMYLQWSA-N 0.000 description 1
- 229960003165 vancomycin Drugs 0.000 description 1
- MYPYJXKWCTUITO-UHFFFAOYSA-N vancomycin Natural products O1C(C(=C2)Cl)=CC=C2C(O)C(C(NC(C2=CC(O)=CC(O)=C2C=2C(O)=CC=C3C=2)C(O)=O)=O)NC(=O)C3NC(=O)C2NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(CC(C)C)NC)C(O)C(C=C3Cl)=CC=C3OC3=CC2=CC1=C3OC1OC(CO)C(O)C(O)C1OC1CC(C)(N)C(O)C(C)O1 MYPYJXKWCTUITO-UHFFFAOYSA-N 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
- 108700026220 vif Genes Proteins 0.000 description 1
- 238000012800 visualization Methods 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
- C12N15/8275—Glyphosate
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
- C12N15/8277—Phosphinotricin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/04—Plant cells or tissues
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
- C12Y114/11—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors (1.14.11)
- C12Y114/11017—Taurine dioxygenase (1.14.11.17)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y203/00—Acyltransferases (2.3)
- C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
- C12Y203/01183—Phosphinothricin acetyltransferase (2.3.1.183)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y205/00—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
- C12Y205/01—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
- C12Y205/01019—3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Physiology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Catching Or Destruction (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161507444P | 2011-07-13 | 2011-07-13 | |
| US61/507,444 | 2011-07-13 | ||
| US201161515634P | 2011-08-05 | 2011-08-05 | |
| US61/515,634 | 2011-08-05 | ||
| PCT/US2012/046706 WO2013010094A1 (en) | 2011-07-13 | 2012-07-13 | Stacked herbicide tolerance event 8264.42.32.1, related transgenic soybean lines, and detection thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU2012280941A1 AU2012280941A1 (en) | 2014-02-06 |
| AU2012280941B2 true AU2012280941B2 (en) | 2017-06-22 |
Family
ID=47506575
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2012280941A Ceased AU2012280941B2 (en) | 2011-07-13 | 2012-07-13 | Stacked herbicide tolerance event 8264.42.32.1, related transgenic soybean lines, and detection thereof |
Country Status (18)
| Country | Link |
|---|---|
| US (1) | US9732353B2 (enExample) |
| EP (1) | EP2731419A4 (enExample) |
| JP (1) | JP6076974B2 (enExample) |
| KR (1) | KR20140044388A (enExample) |
| CN (2) | CN107603978A (enExample) |
| AR (1) | AR087185A1 (enExample) |
| AU (1) | AU2012280941B2 (enExample) |
| BR (1) | BR102012017486A2 (enExample) |
| CA (1) | CA2841543A1 (enExample) |
| CL (1) | CL2014000093A1 (enExample) |
| IL (1) | IL230403B (enExample) |
| MX (1) | MX2014000533A (enExample) |
| RU (1) | RU2636021C2 (enExample) |
| TW (1) | TW201317353A (enExample) |
| UA (1) | UA114716C2 (enExample) |
| UY (1) | UY34199A (enExample) |
| WO (1) | WO2013010094A1 (enExample) |
| ZA (1) | ZA201400476B (enExample) |
Families Citing this family (164)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6111200B2 (ja) | 2010-12-03 | 2017-04-05 | ダウ アグロサイエンシィズ エルエルシー | 除草剤耐性イベント８２６４．４４．０６．１のスタック、関連するトランスジェニックダイズ系統、およびその検出 |
| EP2646559A2 (en) | 2010-12-03 | 2013-10-09 | MS Technologies, LLC | Optimized expression of glyphosate resistance encoding nucleic acid molecules in plant cells |
| BR112013015745B1 (pt) | 2010-12-03 | 2021-01-19 | Ms Technologies, Llc | polinucleotídeos referente ao evento de tolerância a herbicida 8291.45.36.2, cassete de expressão, sonda, bem como processos para identificação do evento, determinação da zigosidade, produção de uma planta de soja transgênica e produção de uma proteína em uma célula de planta |
| BR102012017486A2 (pt) | 2011-07-13 | 2015-08-04 | Dow Agrosciences Llc | Evento de tolerância a herbicidas sobreposto 8264.42.32.1, linhagens de soja transgênicas relacionadas e detecção do mesmo |
| BR112015005674B1 (pt) | 2012-09-14 | 2022-09-06 | BASF Agricultural Solutions Seed US LLC | Polipeptídeos recombinantes para conferir tolerância à herbicidas |
| BR112015018113A2 (pt) | 2013-02-11 | 2017-07-18 | Bayer Cropscience Lp | composições compreendendo um agente de controle biológico baseado em streptomyces e um fungicida |
| US20150373973A1 (en) | 2013-02-11 | 2015-12-31 | Bayer Cropscience Lp | Compositions comprising gougerotin and a biological control agent |
| AU2014214634A1 (en) | 2013-02-11 | 2015-08-06 | Bayer Cropscience Lp | Compositions comprising a streptomyces-based biological control agent and an insecticide |
| MX369500B (es) | 2013-03-07 | 2019-11-11 | Athenix Corp | Genes de toxinas y métodos para su uso. |
| BR112015026235A2 (pt) | 2013-04-19 | 2017-10-10 | Bayer Cropscience Ag | método para melhorar a utilização do potencial de produção de plantas transgênicas envolvendo a aplicação de um derivado de ftaldiamida |
| WO2014177514A1 (en) | 2013-04-30 | 2014-11-06 | Bayer Cropscience Ag | Nematicidal n-substituted phenethylcarboxamides |
| TW201507722A (zh) | 2013-04-30 | 2015-03-01 | Bayer Cropscience Ag | 做為殺線蟲劑及殺體內寄生蟲劑的ｎ－（２－鹵素－２－苯乙基）－羧醯胺類 |
| CN106459986B (zh) | 2014-03-11 | 2020-06-30 | 拜耳作物科学有限合伙公司 | Hppd变体和使用方法 |
| WO2015160618A1 (en) | 2014-04-16 | 2015-10-22 | Bayer Cropscience Lp | Compositions comprising ningnanmycin and a biological control agent |
| WO2015160620A1 (en) | 2014-04-16 | 2015-10-22 | Bayer Cropscience Lp | Compositions comprising ningnanmycin and an insecticide |
| WO2015160619A1 (en) | 2014-04-16 | 2015-10-22 | Bayer Cropscience Lp | Compositions comprising ningnanmycin and a fungicide |
| EP3097782A1 (en) | 2015-05-29 | 2016-11-30 | Bayer CropScience Aktiengesellschaft | Methods for controlling phytopathogenic nematodes by combination of fluopyram and biological control agents |
| ES2933673T3 (es) | 2015-09-11 | 2023-02-13 | BASF Agricultural Solutions Seed US LLC | Variantes de HPPD y métodos de uso |
| CN105445218B (zh) * | 2015-11-25 | 2018-12-18 | 中国科学院南京土壤研究所 | 中红外光谱油菜籽蛋白质含量检测自适应模型的建立方法 |
| CN105567682B (zh) * | 2016-01-12 | 2019-01-29 | 吉林省农业科学院 | 转基因大豆事件b4j8049外源插入片段旁侧序列及其应用 |
| CN106086010B (zh) * | 2016-06-18 | 2019-10-18 | 北京大北农科技集团股份有限公司 | 用于检测除草剂耐受性大豆植物dbn9008的核酸序列及其检测方法 |
| CN106119245B (zh) * | 2016-06-18 | 2019-10-18 | 北京大北农科技集团股份有限公司 | 用于检测除草剂耐受性大豆植物dbn9001的核酸序列及其检测方法 |
| CN106086011B (zh) * | 2016-06-18 | 2019-10-18 | 北京大北农科技集团股份有限公司 | 用于检测除草剂耐受性大豆植物dbn9004的核酸序列及其检测方法 |
| MX387173B (es) | 2016-11-23 | 2025-03-18 | BASF Agricultural Solutions Seed US LLC | Genes de toxinas axmi669 y axmi991 y métodos para su uso. |
| CN110312804A (zh) | 2016-12-22 | 2019-10-08 | 巴斯夫农业种子解决方案美国有限责任公司 | 原种事件ee-gm4及在生物样品中用于鉴定该事件的方法和试剂盒 |
| JP2020504609A (ja) | 2016-12-22 | 2020-02-13 | ビーエーエスエフ アグリカルチュラル ソリューションズ シード ユーエス エルエルシー | 有害線虫の防除のためのｃｒｙ１４の使用 |
| AU2017382333B2 (en) | 2016-12-22 | 2024-02-15 | BASF Agricultural Solutions Seed US LLC | Elite event EE-GM5 and methods and kits for identifying such event in biological samples |
| WO2018136611A1 (en) | 2017-01-18 | 2018-07-26 | Bayer Cropscience Lp | Use of bp005 for the control of plant pathogens |
| EP3571303A1 (en) | 2017-01-18 | 2019-11-27 | Basf Agricultural Solutions Seed Us Llc | Bp005 toxin gene and methods for its use |
| BR112019018056A2 (pt) | 2017-03-07 | 2020-08-11 | BASF Agricultural Solutions Seed US LLC | molécula de ácido nucleico recombinante, cassete de expressão, célula hospedeira, plantas, sementes transgênicas, polipeptídeo recombinante, métodos para conferir tolerância e para controlar ervas daninhas, produto de utilidade e uso da sequência de nucleotídeos |
| BR112020008092A2 (pt) | 2017-10-24 | 2020-09-15 | BASF Agricultural Solutions Seed US LLC | método para conferir tolerância a um herbicida e planta de soja transgênica |
| US20210032651A1 (en) | 2017-10-24 | 2021-02-04 | Basf Se | Improvement of herbicide tolerance to hppd inhibitors by down-regulation of putative 4-hydroxyphenylpyruvate reductases in soybean |
| BR112020024615A2 (pt) | 2018-06-04 | 2021-03-02 | Bayer Aktiengesellschaft | benzoilpirazóis bicíclicos de ação herbicida |
| KR20220035939A (ko) | 2019-07-22 | 2022-03-22 | 바이엘 악티엔게젤샤프트 | 살충제로서의 5-아미노-치환된 피라졸 및 트리아졸 |
| BR112022000915A2 (pt) | 2019-07-23 | 2022-05-17 | Bayer Ag | Compostos de heteroaril-triazol como pesticidas |
| EP4003973B1 (en) | 2019-07-23 | 2024-11-20 | Bayer Aktiengesellschaft | Novel heteroaryl-triazole compounds as pesticides |
| EP3701796A1 (en) | 2019-08-08 | 2020-09-02 | Bayer AG | Active compound combinations |
| CN114760842A (zh) | 2019-10-02 | 2022-07-15 | 拜耳公司 | 包含脂肪酸的活性化合物结合物 |
| FI4041721T3 (fi) | 2019-10-09 | 2024-06-03 | Bayer Ag | Uudet heteroaryyli-triatsoli-yhdisteet torjunta-aineina |
| KR20220081359A (ko) | 2019-10-09 | 2022-06-15 | 바이엘 악티엔게젤샤프트 | 살충제로서의 신규 헤테로아릴-트리아졸 화합물 |
| CA3157808A1 (en) | 2019-10-14 | 2021-04-22 | BASF Agricultural Solutions Seed US LLC | Novel insect resistant genes and methods of use |
| EP4045660A4 (en) | 2019-10-14 | 2024-02-28 | Basf Agricultural Solutions Seed Us Llc | NOVEL INSECT RESISTANCE GENES AND METHODS OF USE |
| KR20220098170A (ko) | 2019-11-07 | 2022-07-11 | 바이엘 악티엔게젤샤프트 | 동물 해충 방제를 위한 치환된 술포닐 아미드 |
| WO2021097162A1 (en) | 2019-11-13 | 2021-05-20 | Bayer Cropscience Lp | Beneficial combinations with paenibacillus |
| TW202134226A (zh) | 2019-11-18 | 2021-09-16 | 德商拜耳廠股份有限公司 | 作為殺蟲劑之新穎雜芳基-三唑化合物 |
| EP4061131A1 (en) | 2019-11-18 | 2022-09-28 | Bayer Aktiengesellschaft | Active compound combinations comprising fatty acids |
| TW202136248A (zh) | 2019-11-25 | 2021-10-01 | 德商拜耳廠股份有限公司 | 作為殺蟲劑之新穎雜芳基-三唑化合物 |
| CN110885898B (zh) * | 2020-01-09 | 2022-06-10 | 福建中医药大学 | 鉴别皱果苋与2种常见混淆品的分子特异性标记引物及方法 |
| CA3165291A1 (en) | 2020-01-31 | 2021-08-05 | Pairwise Plants Services, Inc. | Suppression of shade avoidance response in plants |
| EP4107151A1 (en) | 2020-02-18 | 2022-12-28 | Bayer Aktiengesellschaft | Heteroaryl-triazole compounds as pesticides |
| EP3708565A1 (en) | 2020-03-04 | 2020-09-16 | Bayer AG | Pyrimidinyloxyphenylamidines and the use thereof as fungicides |
| WO2021209490A1 (en) | 2020-04-16 | 2021-10-21 | Bayer Aktiengesellschaft | Cyclaminephenylaminoquinolines as fungicides |
| MX2022012878A (es) | 2020-04-16 | 2022-12-08 | Pairwise Plants Services Inc | Metodos para controlar el tama?o del meristema para la mejora de cultivos. |
| CN115715290A (zh) | 2020-04-21 | 2023-02-24 | 拜耳公司 | 作为农药的2-(杂)芳基取代的稠杂环衍生物 |
| EP4146628A1 (en) | 2020-05-06 | 2023-03-15 | Bayer Aktiengesellschaft | Pyridine (thio)amides as fungicidal compounds |
| TWI891782B (zh) | 2020-05-06 | 2025-08-01 | 德商拜耳廠股份有限公司 | 作為殺蟲劑之新穎雜芳基三唑化合物 |
| EP4149929A1 (en) | 2020-05-12 | 2023-03-22 | Bayer Aktiengesellschaft | Triazine and pyrimidine (thio)amides as fungicidal compounds |
| EP4153566A1 (en) | 2020-05-19 | 2023-03-29 | Bayer CropScience Aktiengesellschaft | Azabicyclic(thio)amides as fungicidal compounds |
| BR112022024489A2 (pt) | 2020-06-02 | 2023-01-31 | Pairwise Plants Services Inc | Métodos para o controle do tamanho do meristema para melhora da cultura agrícola |
| BR112022024394A2 (pt) | 2020-06-04 | 2023-01-31 | Bayer Ag | Heterociclil pirimidinas e triazinas como novos fungicidas |
| JP7729845B2 (ja) | 2020-06-10 | 2025-08-26 | バイエル、アクチエンゲゼルシャフト | 新規殺菌剤としてのアザビシクリル置換複素環 |
| CN115943212A (zh) | 2020-06-17 | 2023-04-07 | 成对植物服务股份有限公司 | 控制分生组织大小以改良作物的方法 |
| CA3187296A1 (en) | 2020-06-18 | 2021-12-23 | Bayer Aktiengesellschaft | 3-(pyridazin-4-yl)-5,6-dihydro-4h-1,2,4-oxadiazine derivatives as fungicides for crop protection |
| CA3187291A1 (en) | 2020-06-18 | 2021-12-23 | Bayer Aktiengesellschaft | Composition for use in agriculture |
| BR112022025710A2 (pt) | 2020-06-19 | 2023-03-07 | Bayer Ag | 1,3,4-oxadiazol pirimidinas e 1,3,4-oxadiazol piridinas como fungicidas |
| WO2021255091A1 (en) | 2020-06-19 | 2021-12-23 | Bayer Aktiengesellschaft | 1,3,4-oxadiazoles and their derivatives as fungicides |
| UY39276A (es) | 2020-06-19 | 2022-01-31 | Bayer Ag | Uso de compuestos de 1,3,4–oxadiazol–2–ilpirimidina para controlar microorganismos fitopatógenos, métodos de uso y composiciones. |
| UY39275A (es) | 2020-06-19 | 2022-01-31 | Bayer Ag | 1,3,4-oxadiazol pirimidinas como fungicidas, procesos e intermediarios para su preparación, métodos de uso y usos de los mismos |
| EP3929189A1 (en) | 2020-06-25 | 2021-12-29 | Bayer Animal Health GmbH | Novel heteroaryl-substituted pyrazine derivatives as pesticides |
| CN116033828B (zh) | 2020-07-02 | 2025-05-16 | 拜耳公司 | 作为害虫防治剂的杂环衍生物 |
| WO2022033991A1 (de) | 2020-08-13 | 2022-02-17 | Bayer Aktiengesellschaft | 5-amino substituierte triazole als schädlingsbekämpfungsmittel |
| WO2022053453A1 (de) | 2020-09-09 | 2022-03-17 | Bayer Aktiengesellschaft | Azolcarboxamide als schädlingsbekämpfungsmittel |
| WO2022058327A1 (en) | 2020-09-15 | 2022-03-24 | Bayer Aktiengesellschaft | Substituted ureas and derivatives as new antifungal agents |
| EP3974414A1 (de) | 2020-09-25 | 2022-03-30 | Bayer AG | 5-amino substituierte pyrazole und triazole als schädlingsbekämpfungsmittel |
| EP3915971A1 (en) | 2020-12-16 | 2021-12-01 | Bayer Aktiengesellschaft | Phenyl-s(o)n-phenylamidines and the use thereof as fungicides |
| US20240023551A1 (en) | 2020-12-18 | 2024-01-25 | Bayer Aktiengesellschaft | Use of dhodh inhibitor for controlling resistant phytopathogenic fungi in crops |
| WO2022129190A1 (en) | 2020-12-18 | 2022-06-23 | Bayer Aktiengesellschaft | (hetero)aryl substituted 1,2,4-oxadiazoles as fungicides |
| WO2022129188A1 (en) | 2020-12-18 | 2022-06-23 | Bayer Aktiengesellschaft | 1,2,4-oxadiazol-3-yl pyrimidines as fungicides |
| WO2022129196A1 (en) | 2020-12-18 | 2022-06-23 | Bayer Aktiengesellschaft | Heterobicycle substituted 1,2,4-oxadiazoles as fungicides |
| EP4036083A1 (de) | 2021-02-02 | 2022-08-03 | Bayer Aktiengesellschaft | 5-oxy substituierte hetereozyklen, als schädlingsbekämpfungsmittel |
| BR112023015909A2 (pt) | 2021-02-11 | 2023-11-21 | Monsanto Technology Llc | Métodos e composições para modificar níveis de citocinina oxidase em plantas |
| WO2022182834A1 (en) | 2021-02-25 | 2022-09-01 | Pairwise Plants Services, Inc. | Methods and compositions for modifying root architecture in plants |
| WO2022207494A1 (en) | 2021-03-30 | 2022-10-06 | Bayer Aktiengesellschaft | 3-(hetero)aryl-5-chlorodifluoromethyl-1,2,4-oxadiazole as fungicide |
| BR112023019788A2 (pt) | 2021-03-30 | 2023-11-07 | Bayer Ag | 3-(hetero)aril-5-clorodifluorometil-1,2,4-oxadiazol como fungicida |
| BR112023022763A2 (pt) | 2021-05-06 | 2024-01-02 | Bayer Ag | Imidazóis anulados substituídos por alquilamida e uso dos mesmos como inseticidas |
| EP4337661A1 (de) | 2021-05-12 | 2024-03-20 | Bayer Aktiengesellschaft | 2-(het)aryl-substituierte kondensierte heterocyclen-derivate als schädlingsbekämpfungsmittel |
| CA3223995A1 (en) | 2021-06-17 | 2022-12-22 | Pairwise Plants Services, Inc. | Modification of growth regulating factor family transcription factors in soybean |
| UY39827A (es) | 2021-06-24 | 2023-01-31 | Pairwise Plants Services Inc | Modificación de genes de ubiquitina ligasa e3 hect para mejorar los rasgos de rendimiento |
| EP4362663A1 (en) | 2021-07-01 | 2024-05-08 | Pairwise Plants Services, Inc. | Methods and compositions for enhancing root system development |
| EP4384626A1 (en) | 2021-08-12 | 2024-06-19 | Pairwise Plants Services, Inc. | Modification of brassinosteroid receptor genes to improve yield traits |
| MX2024001926A (es) | 2021-08-13 | 2024-03-04 | Bayer Ag | Combinaciones de compuestos activos y composiciones fungicidas que las comprenden. |
| UY39902A (es) | 2021-08-17 | 2023-03-31 | Pairwise Plants Services Inc | Métodos y composiciones para modificar genes de histidina quinasa receptores de citoquinina en plant |
| KR20240051198A (ko) | 2021-08-25 | 2024-04-19 | 바이엘 악티엔게젤샤프트 | 농약제로서 신규 피라지닐-트리아졸 화합물 |
| CN118265446A (zh) | 2021-08-30 | 2024-06-28 | 成对植物服务股份有限公司 | 修饰植物中的泛素结合肽酶基因以改善产量性状 |
| AR126938A1 (es) | 2021-09-02 | 2023-11-29 | Pairwise Plants Services Inc | Métodos y composiciones para mejorar la arquitectura de las plantas y los rasgos de rendimiento |
| EP4144739A1 (de) | 2021-09-02 | 2023-03-08 | Bayer Aktiengesellschaft | Anellierte pyrazole als schädlingsbekämpfungsmittel |
| US20230087522A1 (en) | 2021-09-21 | 2023-03-23 | Pairwise Plants Services, Inc. | Methods and compositions for reducing pod shatter in canola |
| WO2023060028A1 (en) | 2021-10-04 | 2023-04-13 | Pairwise Plants Services, Inc. | Methods for improving floret fertility and seed yield |
| WO2023060152A2 (en) | 2021-10-07 | 2023-04-13 | Pairwise Plants Services, Inc. | Methods for improving floret fertility and seed yield |
| EP4426677A1 (en) | 2021-11-03 | 2024-09-11 | Bayer Aktiengesellschaft | Bis(hetero)aryl thioether (thio)amides as fungicidal compounds |
| CN118317956A (zh) | 2021-11-30 | 2024-07-09 | 拜耳公司 | 作为杀真菌化合物的双(杂)芳基硫醚噁二嗪 |
| UY40060A (es) | 2021-12-09 | 2023-07-14 | Pairwise Plants Services Inc | Métodos para mejorar la fertilidad de floretes y el rendimiento de semillas |
| AR128372A1 (es) | 2022-01-31 | 2024-04-24 | Pairwise Plants Services Inc | Supresión de la respuesta de evitación de la sombra en las plantas |
| PE20250350A1 (es) | 2022-02-01 | 2025-02-06 | Globachem Nv | Metodos y composiciones para el control de plagas |
| US20250127163A1 (en) | 2022-02-01 | 2025-04-24 | Globachem Nv | Methods and compositions for controlling pests in soybean |
| MX2024010647A (es) | 2022-03-02 | 2024-09-06 | Pairwise Plants Services Inc | Modificacion de genes del receptor de brasinoesteroides para mejorar rasgos de rendimiento. |
| PE20250266A1 (es) | 2022-03-31 | 2025-01-29 | Pairwise Plants Services Inc | Plantas rosaceae de floracion temprana con caracteristicas mejoradas |
| CN119301147A (zh) | 2022-04-07 | 2025-01-10 | 成对植物服务股份有限公司 | 提高对镰孢菌头枯病的抗性的方法和组合物 |
| EP4511387A1 (en) | 2022-04-21 | 2025-02-26 | Pairwise Plants Services, Inc. | Methods and compositions for improving yield traits |
| WO2023215704A1 (en) | 2022-05-02 | 2023-11-09 | Pairwise Plants Services, Inc. | Methods and compositions for enhancing yield and disease resistance |
| JP2025516326A (ja) | 2022-05-03 | 2025-05-27 | バイエル、アクチエンゲゼルシャフト | （５ｓ）－３－［３－（３－クロロ－２－フルオロフェノキシ）－６－メチルピリダジン－４－イル］－５－（２－クロロ－４－メチルベンジル）－５，６－ジヒドロ－４ｈ－１，２，４－オキサジアジンの結晶形態 |
| IL316251A (en) | 2022-05-03 | 2024-12-01 | Bayer Ag | Use of (5S)-3-[3-(3-chloro-2-fluorophenoxy)-6-methylpyridazin-4-yl]-5-(2-chloro-4-methylbenzyl)-6,5-dihydro-4H-4,2,1-oxadiazine for the control of undesirable microorganisms |
| US20230416767A1 (en) | 2022-05-05 | 2023-12-28 | Pairwise Plants Services, Inc. | Methods and compositions for modifying root architecture and/or improving plant yield traits |
| UY40326A (es) | 2022-06-27 | 2023-12-29 | Pairwise Plants Services Inc | Métodos y composiciones para modificar el escape a la sombra en plantas |
| WO2024006792A1 (en) | 2022-06-29 | 2024-01-04 | Pairwise Plants Services, Inc. | Methods and compositions for controlling meristem size for crop improvement |
| US20240000031A1 (en) | 2022-06-29 | 2024-01-04 | Pairwise Plants Services, Inc. | Methods and compositions for controlling meristem size for crop improvement |
| CN119677724A (zh) | 2022-07-21 | 2025-03-21 | 先正达农作物保护股份公司 | 1,2,4-噁二唑杀真菌剂的结晶形式 |
| UY40384A (es) | 2022-08-04 | 2024-02-29 | Pairwise Plants Services Inc | Métodos y composiciones para mejorar rasgos de rendimiento |
| WO2024033374A1 (en) | 2022-08-11 | 2024-02-15 | Syngenta Crop Protection Ag | Novel arylcarboxamide or arylthioamide compounds |
| CN119654065A (zh) | 2022-08-11 | 2025-03-18 | 成对植物服务股份有限公司 | 用于控制分生组织大小以进行作物改良的方法和组合物 |
| UY40418A (es) | 2022-09-08 | 2024-04-02 | Pairwise Plants Services Inc | Métodos y composiciones para mejorar características de rendimiento en plantas |
| WO2024068518A1 (en) | 2022-09-28 | 2024-04-04 | Bayer Aktiengesellschaft | 3-heteroaryl-5-chlorodifluoromethyl-1,2,4-oxadiazole as fungicide |
| WO2024068520A1 (en) | 2022-09-28 | 2024-04-04 | Bayer Aktiengesellschaft | 3-(hetero)aryl-5-chlorodifluoromethyl-1,2,4-oxadiazole as fungicide |
| EP4295688A1 (en) | 2022-09-28 | 2023-12-27 | Bayer Aktiengesellschaft | Active compound combination |
| GB202214203D0 (en) | 2022-09-28 | 2022-11-09 | Syngenta Crop Protection Ag | Fungicidal compositions |
| WO2024068517A1 (en) | 2022-09-28 | 2024-04-04 | Bayer Aktiengesellschaft | 3-(hetero)aryl-5-chlorodifluoromethyl-1,2,4-oxadiazole as fungicide |
| GB202214202D0 (en) | 2022-09-28 | 2022-11-09 | Syngenta Crop Protection Ag | Agricultural methods |
| WO2024068519A1 (en) | 2022-09-28 | 2024-04-04 | Bayer Aktiengesellschaft | 3-(hetero)aryl-5-chlorodifluoromethyl-1,2,4-oxadiazole as fungicide |
| WO2024100069A1 (en) | 2022-11-08 | 2024-05-16 | Syngenta Crop Protection Ag | Microbiocidal pyridine derivatives |
| EP4385326A1 (en) | 2022-12-15 | 2024-06-19 | Kimitec Biogorup | Biopesticide composition and method for controlling and treating broad spectrum of pests and diseases in plants |
| CN120380151A (zh) | 2022-12-19 | 2025-07-25 | 巴斯夫农业解决方案美国有限责任公司 | 昆虫毒素基因及其使用方法 |
| US20240279673A1 (en) | 2023-02-16 | 2024-08-22 | Pairwise Plants Services, Inc. | Methods and compositions for modifying shade avoidance in plants |
| AR132019A1 (es) | 2023-03-02 | 2025-05-21 | Pairwise Plants Services Inc | Métodos y composiciones para modificar la evitación de la sombra en plantas |
| AR132071A1 (es) | 2023-03-09 | 2025-05-21 | Pairwise Plants Services Inc | Modificación de genes de la vía de señalización de brasinoesteroide para mejorar rasgos de rendimiento en plantas |
| UY40746A (es) | 2023-05-18 | 2024-12-13 | Pairwise Plants Services Inc | Métodos y composiciones para mejorar las características de rendimiento de las plantas |
| WO2025008447A1 (en) | 2023-07-05 | 2025-01-09 | Bayer Aktiengesellschaft | Composition for use in agriculture |
| WO2025008446A1 (en) | 2023-07-05 | 2025-01-09 | Bayer Aktiengesellschaft | Composition for use in agriculture |
| UY40814A (es) | 2023-07-07 | 2024-12-31 | BASF Agricultural Solutions Seed US LLC | Uso de genes novedosos para el control de plagas de nematodos |
| AR133164A1 (es) | 2023-07-18 | 2025-09-03 | Pairwise Plants Services Inc | Métodos y composiciones para modificar la arquitectura radicular en plantas |
| AR133310A1 (es) | 2023-07-27 | 2025-09-17 | Pairwise Plants Services Inc | Métodos y composiciones para modificar rasgos de rendimiento de las plantas |
| WO2025026738A1 (en) | 2023-07-31 | 2025-02-06 | Bayer Aktiengesellschaft | 6-[5-(ethylsulfonyl)-1-methyl-1h-imidazol-4-yl]-7-methyl-3-(pentafluoroethyl)-7h-imidazo[4,5-c]pyridazine derivatives as pesticides |
| WO2025031913A1 (en) | 2023-08-04 | 2025-02-13 | Syngenta Crop Protection Ag | Methods of controlling or preventing infestation of plants by the phytopathogenic microorganism corynespora cassiicola |
| WO2025031989A1 (en) | 2023-08-04 | 2025-02-13 | Syngenta Crop Protection Ag | Methods of controlling or preventing infestation of plants by the phytopathogenic microorganism corynespora cassiicola |
| WO2025031990A1 (en) | 2023-08-04 | 2025-02-13 | Syngenta Crop Protection Ag | Methods of controlling or preventing infestation of soybean plants by the phytopathogenic microorganism corynespora cassiicola |
| WO2025031668A1 (en) | 2023-08-09 | 2025-02-13 | Bayer Aktiengesellschaft | Azaheterobiaryl-substituted 4,5-dihydro-1h-2,4,5-oxadiazines as novel fungicides |
| WO2025032038A1 (en) | 2023-08-09 | 2025-02-13 | Bayer Aktiengesellschaft | Pyridazin-4-yloxadiazines as novel fungicides |
| WO2025064734A1 (en) | 2023-09-21 | 2025-03-27 | Pairwise Plants Services, Inc. | Early flowering black raspberry plants with improved characteristics |
| WO2025078128A1 (en) | 2023-10-11 | 2025-04-17 | Bayer Aktiengesellschaft | Pyridazin-3-one-4-yloxadiazines as novel fungicides |
| WO2025080600A1 (en) | 2023-10-11 | 2025-04-17 | Pairwise Plants Services, Inc. | Methods and compositions for improving crop yield traits |
| WO2025090606A1 (en) | 2023-10-27 | 2025-05-01 | Basf Agricultural Solutions Us Llc | Use of novel genes for the control of nematode pests |
| WO2025098874A1 (en) | 2023-11-10 | 2025-05-15 | Bayer Aktiengesellschaft | Active compound combinations having fungicidal/insecticidal/acaricidal properties |
| WO2025098876A1 (en) | 2023-11-10 | 2025-05-15 | Bayer Aktiengesellschaft | Active compound combinations having insecticidal/acaricidal properties |
| WO2025098875A1 (en) | 2023-11-10 | 2025-05-15 | Bayer Aktiengesellschaft | Active compound combinations having insecticidal/acaricidal properties |
| WO2025120070A1 (en) | 2023-12-08 | 2025-06-12 | Syngenta Crop Protection Ag | Polymorphs of a methoxyacrylate derivative |
| WO2025125639A1 (en) | 2023-12-13 | 2025-06-19 | Syngenta Crop Protection Ag | Method of pathogen control in soybean |
| WO2025168620A1 (en) | 2024-02-07 | 2025-08-14 | Bayer Aktiengesellschaft | Heteroaryl-substituted 4,5-dihydro-1h-2,4,5-oxadiazines as novel fungicides |
| WO2025178902A1 (en) | 2024-02-22 | 2025-08-28 | Pairwise Plants Services, Inc. | Methods and compositions for improving yield characteristics in plants |
| WO2025186065A1 (en) | 2024-03-05 | 2025-09-12 | Bayer Aktiengesellschaft | Heteroaryl-substituted (aza)quinoxaline derivatives as pesticides |
| WO2025190927A1 (en) | 2024-03-14 | 2025-09-18 | Bayer Aktiengesellschaft | Active compound combinations having insecticidal/acaricidal properties |
| WO2025202499A1 (en) | 2024-03-28 | 2025-10-02 | Syngenta Crop Protection Ag | Fungicidal compositions |
| WO2025202482A1 (en) | 2024-03-28 | 2025-10-02 | Syngenta Crop Protection Ag | Fungicidal compositions |
| EP4652843A1 (en) | 2024-05-21 | 2025-11-26 | Kimitec Biogroup S.L | Biopesticide composition, procedure of obtain thereof, and method for controlling and treating broad spectrum of pests in plants |
| EP4652842A1 (en) | 2024-05-21 | 2025-11-26 | Kimitec Biogroup S.L | Biopesticide composition, procedure of obtain thereof, and method for controlling and treating broad spectrum of pests, diseases and weeds in plants |
| CN120591459B (zh) * | 2025-08-05 | 2025-10-21 | 隆平生物技术(海南)有限公司 | 转基因大豆事件lp689-1及其检测方法 |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070083945A1 (en) * | 2000-03-10 | 2007-04-12 | Byrum Joseph R | Nucleic acid molecules and other molecules associated with plants |
Family Cites Families (46)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030237110A9 (en) | 1998-05-12 | 2003-12-25 | Incyte Pharmaceuticals, Inc. | Polynucleotides and polypeptides derived from corn seedling |
| US20040031072A1 (en) | 1999-05-06 | 2004-02-12 | La Rosa Thomas J. | Soy nucleic acid molecules and other molecules associated with transcription plants and uses thereof for plant improvement |
| US7834146B2 (en) | 2000-05-08 | 2010-11-16 | Monsanto Technology Llc | Recombinant polypeptides associated with plants |
| US20030031757A1 (en) | 2001-08-03 | 2003-02-13 | Kraft Food Holdings, Inc. | Stable and bioavailable iron fortified beverages |
| WO2003017782A1 (en) | 2001-08-22 | 2003-03-06 | Bunge Alimentos S.A. | Soybean meal with a reduced fat and soluble sugar content, and methods of making and using the same |
| JP2004027091A (ja) * | 2002-06-27 | 2004-01-29 | Nisshin Oillio Ltd | 圧搾大豆油及びその製造方法 |
| US7705216B2 (en) | 2002-07-29 | 2010-04-27 | Monsanto Technology Llc | Corn event PV-ZMIR13 (MON863) plants and compositions and methods for detection thereof |
| US7045684B1 (en) | 2002-08-19 | 2006-05-16 | Mertec, Llc | Glyphosate-resistant plants |
| CN101173273B (zh) | 2003-02-18 | 2013-03-20 | 孟山都技术有限公司 | 抗草甘磷的i型5-烯醇丙酮酸莽草酸-3-磷酸合酶 |
| EP2281895B1 (en) | 2003-09-29 | 2018-01-03 | Monsanto Technology, LLC | Methods for enhancing stress tolerance in plants and compositions thereof |
| FR2865738B1 (fr) | 2004-02-03 | 2008-06-20 | Agronomique Inst Nat Rech | Utilisation de genes codant des canaux potassiques pour modifier un phenotype relatif a une taille d'au moins un organe de stockage d'une plante |
| AU2004318788B2 (en) | 2004-03-26 | 2011-12-22 | Corteva Agriscience Llc | Cry1F and Cry1Ac transgenic cotton lines and event-specific identification thereof |
| SI2308976T1 (sl) | 2004-04-30 | 2013-05-31 | Dow Agrosciences Llc | Novi geni z rezistenco na herbicide |
| ATE427995T1 (de) * | 2004-06-07 | 2009-04-15 | Basf Plant Science Gmbh | Verbesserte methode zur transformation von sojabohnen |
| US7750207B2 (en) | 2004-09-01 | 2010-07-06 | Monsanto Technology Llc | Zea mays ribulose bisphosphate carboxylase activase promoter |
| CA2588243C (en) | 2004-09-29 | 2013-06-11 | Pioneer Hi-Bred International, Inc. | Corn event das-59122-7 and methods for detection thereof |
| CN101090971B (zh) | 2004-10-29 | 2013-01-02 | 拜尔作物科学公司 | 耐受胁迫的棉花植物 |
| EP3078749B1 (en) * | 2004-12-21 | 2019-10-09 | Monsanto Technology LLC | Transgenic plants with enhanced agronomic traits |
| PT1871901E (pt) | 2005-04-11 | 2011-09-29 | Bayer Bioscience Nv | Acontecimento elite a5547-127 e processos e kits para identificar um tal acontecimento em amostras biológicas |
| PT1885176T (pt) | 2005-05-27 | 2016-11-28 | Monsanto Technology Llc | Evento mon89788 de soja e métodos para a sua deteção |
| CN101410524A (zh) | 2005-08-24 | 2009-04-15 | 先锋高级育种国际公司 | 表达目标多核苷酸的方法和组合物 |
| US8243272B2 (en) * | 2005-09-19 | 2012-08-14 | Jmar Llc | Systems and methods for detecting normal levels of bacteria in water using a multiple angle light scattering (MALS) instrument |
| WO2011066360A1 (en) * | 2009-11-24 | 2011-06-03 | Dow Agrosciences Llc | Detection of aad-12 soybean event 416 |
| EP2484202B1 (en) | 2005-10-28 | 2017-06-07 | Dow AgroSciences LLC | Novel herbicide resistance genes |
| US7951995B2 (en) * | 2006-06-28 | 2011-05-31 | Pioneer Hi-Bred International, Inc. | Soybean event 3560.4.3.5 and compositions and methods for the identification and detection thereof |
| US10752913B2 (en) * | 2006-12-07 | 2020-08-25 | Dow Agrosciences Llc | Selectable marker genes |
| CN100558902C (zh) | 2007-01-24 | 2009-11-11 | 中国农业科学院油料作物研究所 | 转基因油菜外源基因整合事件Rf1外源插入载体旁侧序列及其应用 |
| WO2008095066A2 (en) * | 2007-01-31 | 2008-08-07 | The Curators Of The University Of Missouri | Auto-regulated expression of bacterial isopentenyltransferase gene promotes t-dna transformaton in soybean |
| US7943825B2 (en) * | 2007-04-12 | 2011-05-17 | Iowa State University Research Foundation, Inc. | Metacaspase II in engineering soybean for disease resistance |
| AU2008251445B2 (en) | 2007-05-09 | 2014-04-03 | Corteva Agriscience Llc | Novel herbicide resistance genes |
| JP2008295322A (ja) | 2007-05-30 | 2008-12-11 | Cb:Kk | 食用種子加工物 |
| CN101939435A (zh) | 2007-09-21 | 2011-01-05 | 巴斯夫植物科学有限公司 | 具有增加的产量的植物 |
| WO2009046384A1 (en) | 2007-10-05 | 2009-04-09 | Dow Agrosciences Llc | Methods for transferring molecular substances into plant cells |
| CA2714460C (en) | 2008-03-03 | 2016-05-17 | Ms Technologies Llc | Antibodies immunoreactive with mutant 5-enolpyruvylshikimate-3-phosphate synthase |
| WO2009152359A2 (en) * | 2008-06-11 | 2009-12-17 | Dow Agrosciences Llc | Constructs for expressing herbicide tolerance genes, related plants, and related trait combinations |
| CN106995819B (zh) | 2008-07-01 | 2020-10-20 | 孟山都技术公司 | 用于调控靶基因表达的重组dna构建体和方法 |
| US8344209B2 (en) | 2008-07-14 | 2013-01-01 | Syngenta Participations Ag | Plant regulatory sequences |
| EP2310487A2 (en) | 2008-08-05 | 2011-04-20 | DSM IP Assets B.V. | Adipoyl-7-adca producing strains |
| WO2010079032A1 (en) | 2008-12-17 | 2010-07-15 | Basf Plant Science Gmbh | Production of ketocarotenoids in plants |
| US8293533B2 (en) * | 2008-12-19 | 2012-10-23 | E.I. Du Pont De Nemours And Company | Site-specific integration and stacking of transgenes in soybean via DNA recombinase mediated cassette exchange |
| EP3156489B1 (en) * | 2009-01-22 | 2019-03-06 | Syngenta Participations Ag | Mutant hydroxyphenylpyruvate dioxygenase polypeptides and methods of use |
| PT2503873T (pt) * | 2009-11-23 | 2017-05-18 | Bayer Cropscience Nv | Plantas de soja tolerantes a herbicida e métodos para identificar as mesmas |
| US8916751B2 (en) | 2009-11-24 | 2014-12-23 | Dow Agrosciences, Llc | Control of AAD dicot volunteers in monocot crops |
| CN104928256A (zh) * | 2009-11-24 | 2015-09-23 | 陶氏益农公司 | Aad-12事件416、相关的转基因大豆系及其事件特异性鉴定 |
| JP6111200B2 (ja) | 2010-12-03 | 2017-04-05 | ダウ アグロサイエンシィズ エルエルシー | 除草剤耐性イベント８２６４．４４．０６．１のスタック、関連するトランスジェニックダイズ系統、およびその検出 |
| BR102012017486A2 (pt) | 2011-07-13 | 2015-08-04 | Dow Agrosciences Llc | Evento de tolerância a herbicidas sobreposto 8264.42.32.1, linhagens de soja transgênicas relacionadas e detecção do mesmo |
-
2012
- 2012-07-13 BR BRBR102012017486-3A patent/BR102012017486A2/pt not_active Application Discontinuation
- 2012-07-13 WO PCT/US2012/046706 patent/WO2013010094A1/en not_active Ceased
- 2012-07-13 CA CA2841543A patent/CA2841543A1/en not_active Abandoned
- 2012-07-13 EP EP12810810.7A patent/EP2731419A4/en not_active Withdrawn
- 2012-07-13 UY UY0001034199A patent/UY34199A/es not_active Application Discontinuation
- 2012-07-13 CN CN201711009260.5A patent/CN107603978A/zh active Pending
- 2012-07-13 KR KR1020147003370A patent/KR20140044388A/ko not_active Withdrawn
- 2012-07-13 CN CN201280044567.8A patent/CN103826443B/zh not_active Expired - Fee Related
- 2012-07-13 UA UAA201401402A patent/UA114716C2/uk unknown
- 2012-07-13 AU AU2012280941A patent/AU2012280941B2/en not_active Ceased
- 2012-07-13 US US13/548,720 patent/US9732353B2/en active Active
- 2012-07-13 TW TW101125296A patent/TW201317353A/zh unknown
- 2012-07-13 JP JP2014520377A patent/JP6076974B2/ja not_active Expired - Fee Related
- 2012-07-13 AR ARP120102551A patent/AR087185A1/es unknown
- 2012-07-13 MX MX2014000533A patent/MX2014000533A/es unknown
- 2012-07-13 RU RU2014105285A patent/RU2636021C2/ru active
-
2014
- 2014-01-12 IL IL230403A patent/IL230403B/en not_active IP Right Cessation
- 2014-01-13 CL CL2014000093A patent/CL2014000093A1/es unknown
- 2014-01-20 ZA ZA2014/00476A patent/ZA201400476B/en unknown
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070083945A1 (en) * | 2000-03-10 | 2007-04-12 | Byrum Joseph R | Nucleic acid molecules and other molecules associated with plants |
Non-Patent Citations (1)
| Title |
|---|
| Anonymous, "aad-12, 2mepsps, pat, Glycine max (L.) Merr. (DAS44406, OECD UI: DAS-44406-6)", (20110207), AFFRC, URL: http://www.s.affrc.go.jp/docs/commitee/diversity/110415/pdf/siryo_2-2.pdf, (20141210) * |
Also Published As
| Publication number | Publication date |
|---|---|
| RU2014105285A (ru) | 2015-08-20 |
| CA2841543A1 (en) | 2013-01-17 |
| TW201317353A (zh) | 2013-05-01 |
| AR087185A1 (es) | 2014-02-26 |
| JP2014523251A (ja) | 2014-09-11 |
| WO2013010094A1 (en) | 2013-01-17 |
| AU2012280941A1 (en) | 2014-02-06 |
| EP2731419A1 (en) | 2014-05-21 |
| CN107603978A (zh) | 2018-01-19 |
| JP6076974B2 (ja) | 2017-02-08 |
| IL230403B (en) | 2018-04-30 |
| CN103826443A (zh) | 2014-05-28 |
| BR102012017486A2 (pt) | 2015-08-04 |
| CL2014000093A1 (es) | 2014-11-21 |
| MX2014000533A (es) | 2015-01-14 |
| KR20140044388A (ko) | 2014-04-14 |
| ZA201400476B (en) | 2015-09-30 |
| RU2636021C2 (ru) | 2017-11-17 |
| CN103826443B (zh) | 2017-12-19 |
| US9732353B2 (en) | 2017-08-15 |
| US20130055453A1 (en) | 2013-02-28 |
| UY34199A (es) | 2013-02-28 |
| UA114716C2 (uk) | 2017-07-25 |
| EP2731419A4 (en) | 2015-04-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US12252721B2 (en) | Stacked herbicide tolerance event 8264.44.06.1, related transgenic soybean lines, and detection thereof | |
| AU2012280941B2 (en) | Stacked herbicide tolerance event 8264.42.32.1, related transgenic soybean lines, and detection thereof | |
| US9540656B2 (en) | Stacked herbicide tolerance event 8291.45.36.2, related transgenic soybean lines, and detection thereof | |
| US8785728B2 (en) | AAD-12 event 1606 and related transgenic soybean lines | |
| WO2012033808A2 (en) | Methods for determining the presence or zygosity of aad-12 soybean event 1606 | |
| HK1261733A1 (en) | Stacked herbicide tolerance event 8264.44.06.1, related transgenic soybean lines, and detection thereof | |
| BR122019014603B1 (pt) | Polinucleotídeos referente ao evento de tolerância a herbicida empilhado 8264.44.06.1 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FGA | Letters patent sealed or granted (standard patent) | ||
| MK14 | Patent ceased section 143(a) (annual fees not paid) or expired |