AU2012255988B2 - Photothermal substrates for selective transfection of cells - Google Patents
Photothermal substrates for selective transfection of cells Download PDFInfo
- Publication number
- AU2012255988B2 AU2012255988B2 AU2012255988A AU2012255988A AU2012255988B2 AU 2012255988 B2 AU2012255988 B2 AU 2012255988B2 AU 2012255988 A AU2012255988 A AU 2012255988A AU 2012255988 A AU2012255988 A AU 2012255988A AU 2012255988 B2 AU2012255988 B2 AU 2012255988B2
- Authority
- AU
- Australia
- Prior art keywords
- cell
- nanoparticles
- cells
- thin film
- laser
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
- 239000000758 substrate Substances 0.000 title claims abstract description 118
- 238000001890 transfection Methods 0.000 title description 48
- 239000002105 nanoparticle Substances 0.000 claims abstract description 195
- 239000010409 thin film Substances 0.000 claims abstract description 132
- 239000000463 material Substances 0.000 claims abstract description 102
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 63
- 239000012530 fluid Substances 0.000 claims abstract description 25
- 238000004891 communication Methods 0.000 claims abstract description 11
- 230000005670 electromagnetic radiation Effects 0.000 claims abstract description 8
- 210000004027 cell Anatomy 0.000 claims description 343
- 238000000034 method Methods 0.000 claims description 115
- 239000002245 particle Substances 0.000 claims description 112
- 239000010936 titanium Substances 0.000 claims description 61
- 229910052751 metal Inorganic materials 0.000 claims description 58
- 239000002184 metal Substances 0.000 claims description 57
- 239000010931 gold Substances 0.000 claims description 55
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims description 48
- 229910052737 gold Inorganic materials 0.000 claims description 47
- 239000011521 glass Substances 0.000 claims description 44
- 238000010438 heat treatment Methods 0.000 claims description 40
- 239000012528 membrane Substances 0.000 claims description 39
- 238000005286 illumination Methods 0.000 claims description 22
- 229910052723 transition metal Inorganic materials 0.000 claims description 22
- 229910052719 titanium Inorganic materials 0.000 claims description 21
- 239000003795 chemical substances by application Substances 0.000 claims description 19
- RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 claims description 18
- 150000007523 nucleic acids Chemical class 0.000 claims description 18
- 108020004707 nucleic acids Proteins 0.000 claims description 17
- 102000039446 nucleic acids Human genes 0.000 claims description 17
- 108090000623 proteins and genes Proteins 0.000 claims description 17
- 239000004065 semiconductor Substances 0.000 claims description 17
- -1 transition metal nitride Chemical class 0.000 claims description 17
- 210000004962 mammalian cell Anatomy 0.000 claims description 16
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 16
- 150000003624 transition metals Chemical class 0.000 claims description 16
- 150000004767 nitrides Chemical class 0.000 claims description 15
- 210000003463 organelle Anatomy 0.000 claims description 15
- 102000004169 proteins and genes Human genes 0.000 claims description 15
- 230000015572 biosynthetic process Effects 0.000 claims description 14
- 229910045601 alloy Inorganic materials 0.000 claims description 13
- 239000000956 alloy Substances 0.000 claims description 13
- 239000004033 plastic Substances 0.000 claims description 13
- 229920003023 plastic Polymers 0.000 claims description 13
- 210000000349 chromosome Anatomy 0.000 claims description 11
- 239000002071 nanotube Substances 0.000 claims description 11
- 239000002096 quantum dot Substances 0.000 claims description 11
- 238000004113 cell culture Methods 0.000 claims description 10
- 230000001427 coherent effect Effects 0.000 claims description 9
- 229910000314 transition metal oxide Inorganic materials 0.000 claims description 8
- 229910052500 inorganic mineral Inorganic materials 0.000 claims description 7
- 150000004706 metal oxides Chemical class 0.000 claims description 7
- 239000011859 microparticle Substances 0.000 claims description 7
- 239000011707 mineral Substances 0.000 claims description 7
- 102000004190 Enzymes Human genes 0.000 claims description 5
- 108090000790 Enzymes Proteins 0.000 claims description 5
- 229910001092 metal group alloy Inorganic materials 0.000 claims description 5
- 229910044991 metal oxide Inorganic materials 0.000 claims description 5
- 239000002110 nanocone Substances 0.000 claims description 5
- 239000002070 nanowire Substances 0.000 claims description 5
- 244000052769 pathogen Species 0.000 claims description 5
- 102000053642 Catalytic RNA Human genes 0.000 claims description 4
- 108090000994 Catalytic RNA Proteins 0.000 claims description 4
- 239000001963 growth medium Substances 0.000 claims description 4
- 230000001939 inductive effect Effects 0.000 claims description 4
- 108091092562 ribozyme Proteins 0.000 claims description 4
- 229910010037 TiAlN Inorganic materials 0.000 claims description 3
- 230000005484 gravity Effects 0.000 claims description 3
- 239000002102 nanobead Substances 0.000 claims description 3
- ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 claims description 2
- 230000001717 pathogenic effect Effects 0.000 claims description 2
- 229910052718 tin Inorganic materials 0.000 claims description 2
- 238000001356 surgical procedure Methods 0.000 abstract description 37
- 239000011148 porous material Substances 0.000 abstract description 13
- 239000010408 film Substances 0.000 description 74
- 210000000170 cell membrane Anatomy 0.000 description 67
- 238000002347 injection Methods 0.000 description 28
- 239000007924 injection Substances 0.000 description 28
- 230000005284 excitation Effects 0.000 description 23
- 239000010410 layer Substances 0.000 description 21
- 238000002474 experimental method Methods 0.000 description 19
- 230000005291 magnetic effect Effects 0.000 description 19
- 238000000520 microinjection Methods 0.000 description 19
- 238000002406 microsurgery Methods 0.000 description 19
- 241000894006 Bacteria Species 0.000 description 18
- 230000003833 cell viability Effects 0.000 description 18
- 238000005520 cutting process Methods 0.000 description 17
- 230000000694 effects Effects 0.000 description 17
- 239000000243 solution Substances 0.000 description 17
- 239000002082 metal nanoparticle Substances 0.000 description 15
- 238000000576 coating method Methods 0.000 description 14
- 230000006378 damage Effects 0.000 description 14
- 238000012576 optical tweezer Methods 0.000 description 14
- 230000008569 process Effects 0.000 description 14
- 230000001464 adherent effect Effects 0.000 description 13
- 238000013459 approach Methods 0.000 description 13
- 239000011248 coating agent Substances 0.000 description 13
- 239000000203 mixture Substances 0.000 description 13
- 229910000510 noble metal Inorganic materials 0.000 description 13
- 230000003287 optical effect Effects 0.000 description 13
- 108020004414 DNA Proteins 0.000 description 12
- 238000004519 manufacturing process Methods 0.000 description 12
- 239000002609 medium Substances 0.000 description 12
- 230000010287 polarization Effects 0.000 description 12
- 239000000126 substance Substances 0.000 description 12
- 239000011324 bead Substances 0.000 description 11
- 230000003834 intracellular effect Effects 0.000 description 11
- 239000013612 plasmid Substances 0.000 description 11
- 229910052710 silicon Inorganic materials 0.000 description 11
- UQSXHKLRYXJYBZ-UHFFFAOYSA-N Iron oxide Chemical compound [Fe]=O UQSXHKLRYXJYBZ-UHFFFAOYSA-N 0.000 description 10
- 238000009792 diffusion process Methods 0.000 description 10
- 230000005684 electric field Effects 0.000 description 10
- 238000012546 transfer Methods 0.000 description 10
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 9
- 150000001413 amino acids Chemical class 0.000 description 9
- 229910052732 germanium Inorganic materials 0.000 description 9
- 230000012010 growth Effects 0.000 description 9
- 239000002086 nanomaterial Substances 0.000 description 9
- KDLHZDBZIXYQEI-UHFFFAOYSA-N palladium Substances [Pd] KDLHZDBZIXYQEI-UHFFFAOYSA-N 0.000 description 9
- 239000010703 silicon Substances 0.000 description 9
- 230000001052 transient effect Effects 0.000 description 9
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 8
- NRTOMJZYCJJWKI-UHFFFAOYSA-N Titanium nitride Chemical compound [Ti]#N NRTOMJZYCJJWKI-UHFFFAOYSA-N 0.000 description 8
- 238000009826 distribution Methods 0.000 description 8
- 238000004880 explosion Methods 0.000 description 8
- 239000002360 explosive Substances 0.000 description 8
- 239000002953 phosphate buffered saline Substances 0.000 description 8
- XJMOSONTPMZWPB-UHFFFAOYSA-M propidium iodide Chemical compound [I-].[I-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CCC[N+](C)(CC)CC)=C1C1=CC=CC=C1 XJMOSONTPMZWPB-UHFFFAOYSA-M 0.000 description 8
- 241000581608 Burkholderia thailandensis Species 0.000 description 7
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 7
- 230000001580 bacterial effect Effects 0.000 description 7
- 239000000872 buffer Substances 0.000 description 7
- 210000002421 cell wall Anatomy 0.000 description 7
- GNPVGFCGXDBREM-UHFFFAOYSA-N germanium atom Chemical compound [Ge] GNPVGFCGXDBREM-UHFFFAOYSA-N 0.000 description 7
- 238000003384 imaging method Methods 0.000 description 7
- 210000004940 nucleus Anatomy 0.000 description 7
- 102000004196 processed proteins & peptides Human genes 0.000 description 7
- 238000006722 reduction reaction Methods 0.000 description 7
- 239000000523 sample Substances 0.000 description 7
- 239000004094 surface-active agent Substances 0.000 description 7
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 description 6
- 238000010521 absorption reaction Methods 0.000 description 6
- JNDMLEXHDPKVFC-UHFFFAOYSA-N aluminum;oxygen(2-);yttrium(3+) Chemical compound [O-2].[O-2].[O-2].[Al+3].[Y+3] JNDMLEXHDPKVFC-UHFFFAOYSA-N 0.000 description 6
- 230000001413 cellular effect Effects 0.000 description 6
- 239000000919 ceramic Substances 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 6
- 230000001276 controlling effect Effects 0.000 description 6
- 238000000151 deposition Methods 0.000 description 6
- 238000000605 extraction Methods 0.000 description 6
- 230000001965 increasing effect Effects 0.000 description 6
- 239000007788 liquid Substances 0.000 description 6
- 239000002923 metal particle Substances 0.000 description 6
- 150000002739 metals Chemical class 0.000 description 6
- 239000002101 nanobubble Substances 0.000 description 6
- 239000002073 nanorod Substances 0.000 description 6
- 230000035515 penetration Effects 0.000 description 6
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 6
- 230000009467 reduction Effects 0.000 description 6
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 6
- 238000003786 synthesis reaction Methods 0.000 description 6
- 229910019901 yttrium aluminum garnet Inorganic materials 0.000 description 6
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 5
- 239000004793 Polystyrene Substances 0.000 description 5
- 229920001486 SU-8 photoresist Polymers 0.000 description 5
- 230000008901 benefit Effects 0.000 description 5
- 230000005779 cell damage Effects 0.000 description 5
- 208000037887 cell injury Diseases 0.000 description 5
- 239000002019 doping agent Substances 0.000 description 5
- 238000001704 evaporation Methods 0.000 description 5
- 230000008020 evaporation Effects 0.000 description 5
- 210000002950 fibroblast Anatomy 0.000 description 5
- 239000007850 fluorescent dye Substances 0.000 description 5
- 239000007789 gas Substances 0.000 description 5
- 230000001678 irradiating effect Effects 0.000 description 5
- 230000000670 limiting effect Effects 0.000 description 5
- 238000012986 modification Methods 0.000 description 5
- 230000004048 modification Effects 0.000 description 5
- 229920002223 polystyrene Polymers 0.000 description 5
- 238000004080 punching Methods 0.000 description 5
- 239000010453 quartz Substances 0.000 description 5
- 230000002829 reductive effect Effects 0.000 description 5
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
- QPLDLSVMHZLSFG-UHFFFAOYSA-N Copper oxide Chemical compound [Cu]=O QPLDLSVMHZLSFG-UHFFFAOYSA-N 0.000 description 4
- 238000004435 EPR spectroscopy Methods 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 108010043121 Green Fluorescent Proteins Proteins 0.000 description 4
- 239000002250 absorbent Substances 0.000 description 4
- 230000002745 absorbent Effects 0.000 description 4
- 239000002253 acid Substances 0.000 description 4
- 239000003463 adsorbent Substances 0.000 description 4
- 229910052799 carbon Inorganic materials 0.000 description 4
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 4
- 238000005229 chemical vapour deposition Methods 0.000 description 4
- 229910052802 copper Inorganic materials 0.000 description 4
- 239000010949 copper Substances 0.000 description 4
- 210000001671 embryonic stem cell Anatomy 0.000 description 4
- 230000005350 ferromagnetic resonance Effects 0.000 description 4
- 238000001476 gene delivery Methods 0.000 description 4
- 239000012535 impurity Substances 0.000 description 4
- 230000010354 integration Effects 0.000 description 4
- 150000002632 lipids Chemical class 0.000 description 4
- 239000000696 magnetic material Substances 0.000 description 4
- 239000002077 nanosphere Substances 0.000 description 4
- 229910052763 palladium Inorganic materials 0.000 description 4
- 230000005298 paramagnetic effect Effects 0.000 description 4
- 230000000737 periodic effect Effects 0.000 description 4
- 229910052709 silver Inorganic materials 0.000 description 4
- 210000000130 stem cell Anatomy 0.000 description 4
- 238000004416 surface enhanced Raman spectroscopy Methods 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 102000007469 Actins Human genes 0.000 description 3
- 108010085238 Actins Proteins 0.000 description 3
- 229920004943 Delrin® Polymers 0.000 description 3
- 241000713666 Lentivirus Species 0.000 description 3
- FOIXSVOLVBLSDH-UHFFFAOYSA-N Silver ion Chemical compound [Ag+] FOIXSVOLVBLSDH-UHFFFAOYSA-N 0.000 description 3
- 229920006362 Teflon® Polymers 0.000 description 3
- 238000002679 ablation Methods 0.000 description 3
- 229910003481 amorphous carbon Inorganic materials 0.000 description 3
- 238000003491 array Methods 0.000 description 3
- 210000004436 artificial bacterial chromosome Anatomy 0.000 description 3
- 238000000541 cathodic arc deposition Methods 0.000 description 3
- 239000006143 cell culture medium Substances 0.000 description 3
- 229910052804 chromium Inorganic materials 0.000 description 3
- 239000011651 chromium Substances 0.000 description 3
- ZMXDDKWLCZADIW-UHFFFAOYSA-N dimethylformamide Substances CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 3
- 238000009501 film coating Methods 0.000 description 3
- 108010021843 fluorescent protein 583 Proteins 0.000 description 3
- 239000012737 fresh medium Substances 0.000 description 3
- 229910052733 gallium Inorganic materials 0.000 description 3
- 239000003112 inhibitor Substances 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Substances [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 3
- 230000001788 irregular Effects 0.000 description 3
- 238000001725 laser pyrolysis Methods 0.000 description 3
- 230000033001 locomotion Effects 0.000 description 3
- 210000004072 lung Anatomy 0.000 description 3
- 239000002069 magnetite nanoparticle Substances 0.000 description 3
- 230000007246 mechanism Effects 0.000 description 3
- 239000011325 microbead Substances 0.000 description 3
- 210000003470 mitochondria Anatomy 0.000 description 3
- 239000002091 nanocage Substances 0.000 description 3
- 239000002064 nanoplatelet Substances 0.000 description 3
- PXHVJJICTQNCMI-UHFFFAOYSA-N nickel Substances [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 3
- 230000037361 pathway Effects 0.000 description 3
- 238000000059 patterning Methods 0.000 description 3
- 229910052697 platinum Inorganic materials 0.000 description 3
- 229920000642 polymer Polymers 0.000 description 3
- 229920001184 polypeptide Polymers 0.000 description 3
- 239000002243 precursor Substances 0.000 description 3
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 3
- 238000005086 pumping Methods 0.000 description 3
- 230000005855 radiation Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 239000004332 silver Substances 0.000 description 3
- SQGYOTSLMSWVJD-UHFFFAOYSA-N silver(1+) nitrate Chemical compound [Ag+].[O-]N(=O)=O SQGYOTSLMSWVJD-UHFFFAOYSA-N 0.000 description 3
- 238000004088 simulation Methods 0.000 description 3
- 239000012279 sodium borohydride Substances 0.000 description 3
- 229910000033 sodium borohydride Inorganic materials 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 238000004544 sputter deposition Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 230000035899 viability Effects 0.000 description 3
- 229910052725 zinc Inorganic materials 0.000 description 3
- 239000011701 zinc Substances 0.000 description 3
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 2
- 108090000672 Annexin A5 Proteins 0.000 description 2
- 102000004121 Annexin A5 Human genes 0.000 description 2
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 2
- 229920002307 Dextran Polymers 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- 235000002918 Fraxinus excelsior Nutrition 0.000 description 2
- GYHNNYVSQQEPJS-UHFFFAOYSA-N Gallium Chemical compound [Ga] GYHNNYVSQQEPJS-UHFFFAOYSA-N 0.000 description 2
- 102000004144 Green Fluorescent Proteins Human genes 0.000 description 2
- 239000002211 L-ascorbic acid Substances 0.000 description 2
- 235000000069 L-ascorbic acid Nutrition 0.000 description 2
- 239000000232 Lipid Bilayer Substances 0.000 description 2
- 108091034117 Oligonucleotide Proteins 0.000 description 2
- KPKZJLCSROULON-QKGLWVMZSA-N Phalloidin Chemical compound N1C(=O)[C@@H]([C@@H](O)C)NC(=O)[C@H](C)NC(=O)[C@H](C[C@@](C)(O)CO)NC(=O)[C@H](C2)NC(=O)[C@H](C)NC(=O)[C@@H]3C[C@H](O)CN3C(=O)[C@@H]1CSC1=C2C2=CC=CC=C2N1 KPKZJLCSROULON-QKGLWVMZSA-N 0.000 description 2
- 238000001069 Raman spectroscopy Methods 0.000 description 2
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 2
- 108091027967 Small hairpin RNA Proteins 0.000 description 2
- 238000003917 TEM image Methods 0.000 description 2
- 150000007513 acids Chemical class 0.000 description 2
- 125000003275 alpha amino acid group Chemical group 0.000 description 2
- 150000001412 amines Chemical class 0.000 description 2
- 125000000539 amino acid group Chemical group 0.000 description 2
- 230000000692 anti-sense effect Effects 0.000 description 2
- 229910052785 arsenic Inorganic materials 0.000 description 2
- RQNWIZPPADIBDY-UHFFFAOYSA-N arsenic atom Chemical compound [As] RQNWIZPPADIBDY-UHFFFAOYSA-N 0.000 description 2
- 229960005070 ascorbic acid Drugs 0.000 description 2
- 239000002956 ash Substances 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 230000008952 bacterial invasion Effects 0.000 description 2
- 229910052796 boron Inorganic materials 0.000 description 2
- 210000004899 c-terminal region Anatomy 0.000 description 2
- 229910052793 cadmium Inorganic materials 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 235000014633 carbohydrates Nutrition 0.000 description 2
- 239000001569 carbon dioxide Substances 0.000 description 2
- 229910002092 carbon dioxide Inorganic materials 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 230000030833 cell death Effects 0.000 description 2
- 238000012512 characterization method Methods 0.000 description 2
- 230000002925 chemical effect Effects 0.000 description 2
- 239000000084 colloidal system Substances 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 238000002716 delivery method Methods 0.000 description 2
- 230000008021 deposition Effects 0.000 description 2
- 239000000975 dye Substances 0.000 description 2
- 238000005566 electron beam evaporation Methods 0.000 description 2
- 238000004520 electroporation Methods 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 230000007717 exclusion Effects 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 239000002223 garnet Substances 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- BHEPBYXIRTUNPN-UHFFFAOYSA-N hydridophosphorus(.) (triplet) Chemical compound [PH] BHEPBYXIRTUNPN-UHFFFAOYSA-N 0.000 description 2
- 238000011065 in-situ storage Methods 0.000 description 2
- 210000004263 induced pluripotent stem cell Anatomy 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- 150000002505 iron Chemical class 0.000 description 2
- 229930027917 kanamycin Natural products 0.000 description 2
- 229960000318 kanamycin Drugs 0.000 description 2
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 2
- 229930182823 kanamycin A Natural products 0.000 description 2
- 210000003734 kidney Anatomy 0.000 description 2
- 230000002147 killing effect Effects 0.000 description 2
- 238000002032 lab-on-a-chip Methods 0.000 description 2
- 210000003712 lysosome Anatomy 0.000 description 2
- 230000001868 lysosomic effect Effects 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000002844 melting Methods 0.000 description 2
- 230000008018 melting Effects 0.000 description 2
- MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 2
- 108091070501 miRNA Proteins 0.000 description 2
- 239000002679 microRNA Substances 0.000 description 2
- 238000001000 micrograph Methods 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 229910052759 nickel Inorganic materials 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 239000002773 nucleotide Substances 0.000 description 2
- 125000003729 nucleotide group Chemical group 0.000 description 2
- 239000013307 optical fiber Substances 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 238000012856 packing Methods 0.000 description 2
- 230000035699 permeability Effects 0.000 description 2
- 229920002120 photoresistant polymer Polymers 0.000 description 2
- 238000005240 physical vapour deposition Methods 0.000 description 2
- 238000006116 polymerization reaction Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 230000008439 repair process Effects 0.000 description 2
- 229910052703 rhodium Inorganic materials 0.000 description 2
- 239000010948 rhodium Substances 0.000 description 2
- 239000011435 rock Substances 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 238000001878 scanning electron micrograph Methods 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 238000010008 shearing Methods 0.000 description 2
- HBMJWWWQQXIZIP-UHFFFAOYSA-N silicon carbide Chemical compound [Si+]#[C-] HBMJWWWQQXIZIP-UHFFFAOYSA-N 0.000 description 2
- 229910010271 silicon carbide Inorganic materials 0.000 description 2
- 239000004055 small Interfering RNA Substances 0.000 description 2
- 210000001082 somatic cell Anatomy 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 230000001360 synchronised effect Effects 0.000 description 2
- 229910052715 tantalum Inorganic materials 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- 230000001960 triggered effect Effects 0.000 description 2
- 239000013598 vector Substances 0.000 description 2
- 229910000859 α-Fe Inorganic materials 0.000 description 2
- YBNMDCCMCLUHBL-UHFFFAOYSA-N (2,5-dioxopyrrolidin-1-yl) 4-pyren-1-ylbutanoate Chemical compound C=1C=C(C2=C34)C=CC3=CC=CC4=CC=C2C=1CCCC(=O)ON1C(=O)CCC1=O YBNMDCCMCLUHBL-UHFFFAOYSA-N 0.000 description 1
- WGFGZNVQMGCHHV-LURJTMIESA-N (2s)-2-amino-5-(2-aminoimidazol-1-yl)pentanoic acid Chemical compound OC(=O)[C@@H](N)CCCN1C=CN=C1N WGFGZNVQMGCHHV-LURJTMIESA-N 0.000 description 1
- VLJQDHDVZJXNQL-UHFFFAOYSA-N 4-methyl-n-(oxomethylidene)benzenesulfonamide Chemical compound CC1=CC=C(S(=O)(=O)N=C=O)C=C1 VLJQDHDVZJXNQL-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 229910017115 AlSb Inorganic materials 0.000 description 1
- 239000012099 Alexa Fluor family Substances 0.000 description 1
- 238000012935 Averaging Methods 0.000 description 1
- 229910015808 BaTe Inorganic materials 0.000 description 1
- 241001453380 Burkholderia Species 0.000 description 1
- 241000418666 Burkholderia thailandensis E264 Species 0.000 description 1
- 229910004813 CaTe Inorganic materials 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 229910004613 CdTe Inorganic materials 0.000 description 1
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 description 1
- 229910021560 Chromium(III) bromide Inorganic materials 0.000 description 1
- 229910003321 CoFe Inorganic materials 0.000 description 1
- 229910017612 Cu(In,Ga)Se2 Inorganic materials 0.000 description 1
- 229910002475 Cu2ZnSnS4 Inorganic materials 0.000 description 1
- 201000010374 Down Syndrome Diseases 0.000 description 1
- 229910017356 Fe2C Inorganic materials 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- MBMLMWLHJBBADN-UHFFFAOYSA-N Ferrous sulfide Chemical compound [Fe]=S MBMLMWLHJBBADN-UHFFFAOYSA-N 0.000 description 1
- 229910017112 Fe—C Inorganic materials 0.000 description 1
- 229910005542 GaSb Inorganic materials 0.000 description 1
- 229910005543 GaSe Inorganic materials 0.000 description 1
- 229910052688 Gadolinium Inorganic materials 0.000 description 1
- 229910001218 Gallium arsenide Inorganic materials 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 229910000673 Indium arsenide Inorganic materials 0.000 description 1
- GPXJNWSHGFTCBW-UHFFFAOYSA-N Indium phosphide Chemical compound [In]#P GPXJNWSHGFTCBW-UHFFFAOYSA-N 0.000 description 1
- 229910001030 Iron–nickel alloy Inorganic materials 0.000 description 1
- 208000017924 Klinefelter Syndrome Diseases 0.000 description 1
- 229910000708 MFe2O4 Inorganic materials 0.000 description 1
- 229910017680 MgTe Inorganic materials 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- 241000699660 Mus musculus Species 0.000 description 1
- 229930040373 Paraformaldehyde Natural products 0.000 description 1
- 229930182555 Penicillin Natural products 0.000 description 1
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
- 108091093037 Peptide nucleic acid Proteins 0.000 description 1
- 108010009711 Phalloidine Proteins 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 239000006146 Roswell Park Memorial Institute medium Substances 0.000 description 1
- 229910052581 Si3N4 Inorganic materials 0.000 description 1
- BLRPTPMANUNPDV-UHFFFAOYSA-N Silane Chemical compound [SiH4] BLRPTPMANUNPDV-UHFFFAOYSA-N 0.000 description 1
- 229910004411 SrTe Inorganic materials 0.000 description 1
- 239000004098 Tetracycline Substances 0.000 description 1
- 208000037280 Trisomy Diseases 0.000 description 1
- 239000013504 Triton X-100 Substances 0.000 description 1
- 229920004890 Triton X-100 Polymers 0.000 description 1
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 238000002441 X-ray diffraction Methods 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 229910007381 Zn3Sb2 Inorganic materials 0.000 description 1
- 229910007709 ZnTe Inorganic materials 0.000 description 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
- CYKMNKXPYXUVPR-UHFFFAOYSA-N [C].[Ti] Chemical compound [C].[Ti] CYKMNKXPYXUVPR-UHFFFAOYSA-N 0.000 description 1
- LBQVQRQFDUVUCX-MJQNIGQHSA-N [[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-4-hydroxy-3-phosphonooxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [(3R)-3-hydroxy-2,2-dimethyl-4-[[3-[2-(3-methylbutylsulfanyl)ethylamino]-3-oxopropyl]amino]-4-oxobutyl] hydrogen phosphate Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSCCC(C)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 LBQVQRQFDUVUCX-MJQNIGQHSA-N 0.000 description 1
- 238000000862 absorption spectrum Methods 0.000 description 1
- 229910052946 acanthite Inorganic materials 0.000 description 1
- 239000000370 acceptor Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000004115 adherent culture Methods 0.000 description 1
- 210000004504 adult stem cell Anatomy 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- PPQRONHOSHZGFQ-LMVFSUKVSA-N aldehydo-D-ribose 5-phosphate Chemical group OP(=O)(O)OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PPQRONHOSHZGFQ-LMVFSUKVSA-N 0.000 description 1
- AZDRQVAHHNSJOQ-UHFFFAOYSA-N alumane Chemical class [AlH3] AZDRQVAHHNSJOQ-UHFFFAOYSA-N 0.000 description 1
- UQZIWOQVLUASCR-UHFFFAOYSA-N alumane;titanium Chemical compound [AlH3].[Ti] UQZIWOQVLUASCR-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- 125000003277 amino group Chemical group 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 150000001495 arsenic compounds Chemical class 0.000 description 1
- 239000012298 atmosphere Substances 0.000 description 1
- 210000003719 b-lymphocyte Anatomy 0.000 description 1
- 229910052788 barium Inorganic materials 0.000 description 1
- 229910002113 barium titanate Inorganic materials 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 239000003738 black carbon Substances 0.000 description 1
- 210000002459 blastocyst Anatomy 0.000 description 1
- 150000001639 boron compounds Chemical class 0.000 description 1
- 239000005388 borosilicate glass Substances 0.000 description 1
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 description 1
- UHYPYGJEEGLRJD-UHFFFAOYSA-N cadmium(2+);selenium(2-) Chemical compound [Se-2].[Cd+2] UHYPYGJEEGLRJD-UHFFFAOYSA-N 0.000 description 1
- DEGAKNSWVGKMLS-UHFFFAOYSA-N calcein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC(CN(CC(O)=O)CC(O)=O)=C(O)C=C1OC1=C2C=C(CN(CC(O)=O)CC(=O)O)C(O)=C1 DEGAKNSWVGKMLS-UHFFFAOYSA-N 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 239000011852 carbon nanoparticle Substances 0.000 description 1
- 239000002041 carbon nanotube Substances 0.000 description 1
- 229910021393 carbon nanotube Inorganic materials 0.000 description 1
- 150000001732 carboxylic acid derivatives Chemical class 0.000 description 1
- 125000002091 cationic group Chemical group 0.000 description 1
- 229960000484 ceftazidime Drugs 0.000 description 1
- NMVPEQXCMGEDNH-TZVUEUGBSA-N ceftazidime pentahydrate Chemical compound O.O.O.O.O.S([C@@H]1[C@@H](C(N1C=1C([O-])=O)=O)NC(=O)\C(=N/OC(C)(C)C(O)=O)C=2N=C(N)SC=2)CC=1C[N+]1=CC=CC=C1 NMVPEQXCMGEDNH-TZVUEUGBSA-N 0.000 description 1
- 230000021164 cell adhesion Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 239000013592 cell lysate Substances 0.000 description 1
- 208000019065 cervical carcinoma Diseases 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000001311 chemical methods and process Methods 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 239000003638 chemical reducing agent Substances 0.000 description 1
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 1
- 229960005091 chloramphenicol Drugs 0.000 description 1
- UZDWIWGMKWZEPE-UHFFFAOYSA-K chromium(iii) bromide Chemical compound [Cr+3].[Br-].[Br-].[Br-] UZDWIWGMKWZEPE-UHFFFAOYSA-K 0.000 description 1
- 230000002759 chromosomal effect Effects 0.000 description 1
- 238000005049 combustion synthesis Methods 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
- 230000005494 condensation Effects 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- DMSZORWOGDLWGN-UHFFFAOYSA-N ctk1a3526 Chemical compound NP(N)(N)=O DMSZORWOGDLWGN-UHFFFAOYSA-N 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 210000004292 cytoskeleton Anatomy 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000003989 dielectric material Substances 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 239000004205 dimethyl polysiloxane Substances 0.000 description 1
- 235000013870 dimethyl polysiloxane Nutrition 0.000 description 1
- NAGJZTKCGNOGPW-UHFFFAOYSA-K dioxido-sulfanylidene-sulfido-$l^{5}-phosphane Chemical compound [O-]P([O-])([S-])=S NAGJZTKCGNOGPW-UHFFFAOYSA-K 0.000 description 1
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 208000035475 disorder Diseases 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 235000012489 doughnuts Nutrition 0.000 description 1
- 238000012377 drug delivery Methods 0.000 description 1
- 238000007876 drug discovery Methods 0.000 description 1
- 238000001312 dry etching Methods 0.000 description 1
- 238000000609 electron-beam lithography Methods 0.000 description 1
- 230000012202 endocytosis Effects 0.000 description 1
- 210000001163 endosome Anatomy 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000005530 etching Methods 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 210000004700 fetal blood Anatomy 0.000 description 1
- 210000000604 fetal stem cell Anatomy 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000002073 fluorescence micrograph Methods 0.000 description 1
- 238000012757 fluorescence staining Methods 0.000 description 1
- 239000000446 fuel Substances 0.000 description 1
- 238000007306 functionalization reaction Methods 0.000 description 1
- 231100000722 genetic damage Toxicity 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 210000004602 germ cell Anatomy 0.000 description 1
- 229910021480 group 4 element Inorganic materials 0.000 description 1
- 229910021478 group 5 element Inorganic materials 0.000 description 1
- 229940093920 gynecological arsenic compound Drugs 0.000 description 1
- 229910052735 hafnium Inorganic materials 0.000 description 1
- 230000017525 heat dissipation Effects 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 150000002431 hydrogen Chemical class 0.000 description 1
- 125000001841 imino group Chemical group [H]N=* 0.000 description 1
- 238000010569 immunofluorescence imaging Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 229910052738 indium Inorganic materials 0.000 description 1
- WPYVAWXEWQSOGY-UHFFFAOYSA-N indium antimonide Chemical compound [Sb]#[In] WPYVAWXEWQSOGY-UHFFFAOYSA-N 0.000 description 1
- RPQDHPTXJYYUPQ-UHFFFAOYSA-N indium arsenide Chemical compound [In]#[As] RPQDHPTXJYYUPQ-UHFFFAOYSA-N 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 210000005061 intracellular organelle Anatomy 0.000 description 1
- 229910052741 iridium Inorganic materials 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- MTRJKZUDDJZTLA-UHFFFAOYSA-N iron yttrium Chemical compound [Fe].[Y] MTRJKZUDDJZTLA-UHFFFAOYSA-N 0.000 description 1
- 238000005304 joining Methods 0.000 description 1
- 210000003292 kidney cell Anatomy 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 229910052746 lanthanum Inorganic materials 0.000 description 1
- 238000000608 laser ablation Methods 0.000 description 1
- 238000004093 laser heating Methods 0.000 description 1
- 238000000651 laser trapping Methods 0.000 description 1
- 208000032839 leukemia Diseases 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 230000002934 lysing effect Effects 0.000 description 1
- 230000002101 lytic effect Effects 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 239000002122 magnetic nanoparticle Substances 0.000 description 1
- 238000001755 magnetron sputter deposition Methods 0.000 description 1
- 229910052960 marcasite Inorganic materials 0.000 description 1
- 230000000873 masking effect Effects 0.000 description 1
- 108010082117 matrigel Proteins 0.000 description 1
- 238000005551 mechanical alloying Methods 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 1
- 229910052753 mercury Inorganic materials 0.000 description 1
- 150000001247 metal acetylides Chemical class 0.000 description 1
- 239000007769 metal material Substances 0.000 description 1
- 239000000693 micelle Substances 0.000 description 1
- 238000002493 microarray Methods 0.000 description 1
- 238000004853 microextraction Methods 0.000 description 1
- 230000003278 mimic effect Effects 0.000 description 1
- 229910003465 moissanite Inorganic materials 0.000 description 1
- 238000001451 molecular beam epitaxy Methods 0.000 description 1
- 229910052961 molybdenite Inorganic materials 0.000 description 1
- CWQXQMHSOZUFJS-UHFFFAOYSA-N molybdenum disulfide Chemical compound S=[Mo]=S CWQXQMHSOZUFJS-UHFFFAOYSA-N 0.000 description 1
- 229910052982 molybdenum disulfide Inorganic materials 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 239000002048 multi walled nanotube Substances 0.000 description 1
- 239000002103 nanocoating Substances 0.000 description 1
- 239000002121 nanofiber Substances 0.000 description 1
- 229910052758 niobium Inorganic materials 0.000 description 1
- 239000002777 nucleoside Substances 0.000 description 1
- 150000003833 nucleoside derivatives Chemical class 0.000 description 1
- CXQXSVUQTKDNFP-UHFFFAOYSA-N octamethyltrisiloxane Chemical compound C[Si](C)(C)O[Si](C)(C)O[Si](C)(C)C CXQXSVUQTKDNFP-UHFFFAOYSA-N 0.000 description 1
- 229960002378 oftasceine Drugs 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 229910052958 orpiment Inorganic materials 0.000 description 1
- 239000007800 oxidant agent Substances 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 229920002866 paraformaldehyde Polymers 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 239000011236 particulate material Substances 0.000 description 1
- 229940049954 penicillin Drugs 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 238000000206 photolithography Methods 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 238000004987 plasma desorption mass spectroscopy Methods 0.000 description 1
- 229910021340 platinum monosilicide Inorganic materials 0.000 description 1
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 239000002861 polymer material Substances 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 229940098458 powder spray Drugs 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 229910052683 pyrite Inorganic materials 0.000 description 1
- NIFIFKQPDTWWGU-UHFFFAOYSA-N pyrite Chemical compound [Fe+2].[S-][S-] NIFIFKQPDTWWGU-UHFFFAOYSA-N 0.000 description 1
- 239000006100 radiation absorber Substances 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 238000006479 redox reaction Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 230000004043 responsiveness Effects 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 229910052702 rhenium Inorganic materials 0.000 description 1
- MHOVAHRLVXNVSD-UHFFFAOYSA-N rhodium atom Chemical compound [Rh] MHOVAHRLVXNVSD-UHFFFAOYSA-N 0.000 description 1
- 150000003290 ribose derivatives Chemical group 0.000 description 1
- 229910052707 ruthenium Inorganic materials 0.000 description 1
- 239000012266 salt solution Substances 0.000 description 1
- SBIBMFFZSBJNJF-UHFFFAOYSA-N selenium;zinc Chemical compound [Se]=[Zn] SBIBMFFZSBJNJF-UHFFFAOYSA-N 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- 229910000077 silane Inorganic materials 0.000 description 1
- HQVNEWCFYHHQES-UHFFFAOYSA-N silicon nitride Chemical compound N12[Si]34N5[Si]62N3[Si]51N64 HQVNEWCFYHHQES-UHFFFAOYSA-N 0.000 description 1
- 229910052814 silicon oxide Inorganic materials 0.000 description 1
- 239000010944 silver (metal) Substances 0.000 description 1
- 229910001961 silver nitrate Inorganic materials 0.000 description 1
- FSJWWSXPIWGYKC-UHFFFAOYSA-M silver;silver;sulfanide Chemical compound [SH-].[Ag].[Ag+] FSJWWSXPIWGYKC-UHFFFAOYSA-M 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- 230000005476 size effect Effects 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- SDKPSXWGRWWLKR-UHFFFAOYSA-M sodium;9,10-dioxoanthracene-1-sulfonate Chemical compound [Na+].O=C1C2=CC=CC=C2C(=O)C2=C1C=CC=C2S(=O)(=O)[O-] SDKPSXWGRWWLKR-UHFFFAOYSA-M 0.000 description 1
- 239000007779 soft material Substances 0.000 description 1
- 230000000392 somatic effect Effects 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 229910052596 spinel Inorganic materials 0.000 description 1
- 239000011029 spinel Substances 0.000 description 1
- 229910052566 spinel group Inorganic materials 0.000 description 1
- 238000005118 spray pyrolysis Methods 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 229910052712 strontium Inorganic materials 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 238000002198 surface plasmon resonance spectroscopy Methods 0.000 description 1
- 238000004381 surface treatment Methods 0.000 description 1
- 230000002459 sustained effect Effects 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 238000010189 synthetic method Methods 0.000 description 1
- GUVRBAGPIYLISA-UHFFFAOYSA-N tantalum atom Chemical compound [Ta] GUVRBAGPIYLISA-UHFFFAOYSA-N 0.000 description 1
- 229940071240 tetrachloroaurate Drugs 0.000 description 1
- 229960002180 tetracycline Drugs 0.000 description 1
- 229930101283 tetracycline Natural products 0.000 description 1
- 235000019364 tetracycline Nutrition 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
- 238000000427 thin-film deposition Methods 0.000 description 1
- 150000003573 thiols Chemical class 0.000 description 1
- RYYWUUFWQRZTIU-UHFFFAOYSA-K thiophosphate Chemical compound [O-]P([O-])([O-])=S RYYWUUFWQRZTIU-UHFFFAOYSA-K 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 238000011830 transgenic mouse model Methods 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 230000008733 trauma Effects 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
- 229910052721 tungsten Inorganic materials 0.000 description 1
- 241001430294 unidentified retrovirus Species 0.000 description 1
- 238000009827 uniform distribution Methods 0.000 description 1
- 229910052720 vanadium Inorganic materials 0.000 description 1
- 238000007740 vapor deposition Methods 0.000 description 1
- 239000003981 vehicle Substances 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 230000010356 wave oscillation Effects 0.000 description 1
- 230000003313 weakening effect Effects 0.000 description 1
- 229910052727 yttrium Inorganic materials 0.000 description 1
- 239000010457 zeolite Substances 0.000 description 1
- 229910052726 zirconium Inorganic materials 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/42—Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/16—Microfluidic devices; Capillary tubes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/20—Material Coatings
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/06—Means for regulation, monitoring, measurement or control, e.g. flow regulation of illumination
- C12M41/08—Means for changing the orientation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Sustainable Development (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Cell Biology (AREA)
- Electromagnetism (AREA)
- Clinical Laboratory Science (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Dispersion Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161486114P | 2011-05-13 | 2011-05-13 | |
| US61/486,114 | 2011-05-13 | ||
| PCT/US2012/037810 WO2012158631A2 (en) | 2011-05-13 | 2012-05-14 | Photothermal substrates for selective transfection of cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU2012255988A1 AU2012255988A1 (en) | 2014-01-09 |
| AU2012255988B2 true AU2012255988B2 (en) | 2017-07-13 |
Family
ID=47177590
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2012255988A Ceased AU2012255988B2 (en) | 2011-05-13 | 2012-05-14 | Photothermal substrates for selective transfection of cells |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US20150044751A1 (enExample) |
| EP (1) | EP2707474B1 (enExample) |
| JP (2) | JP6144255B2 (enExample) |
| KR (1) | KR101968778B1 (enExample) |
| CN (2) | CN103649295B (enExample) |
| AU (1) | AU2012255988B2 (enExample) |
| CA (1) | CA2873204C (enExample) |
| WO (1) | WO2012158631A2 (enExample) |
Families Citing this family (52)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2707474B1 (en) | 2011-05-13 | 2019-07-10 | The Regents of The University of California | Photothermal substrates for selective transfection of cells |
| WO2014151888A1 (en) | 2013-03-15 | 2014-09-25 | The Regents Of The University Of California | High-throughput cargo delivery into live cells using photothermal platforms |
| US9552917B2 (en) * | 2013-09-20 | 2017-01-24 | Skyworks Solutions, Inc. | Materials, devices and methods related to below-resonance radio-frequency circulators and isolators |
| JP6839073B2 (ja) * | 2014-03-28 | 2021-03-03 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニアＴｈｅ Ｒｅｇｅｎｔｓ Ｏｆ Ｔｈｅ Ｕｎｉｖｅｒｓｉｔｙ Ｏｆ Ｃａｌｉｆｏｒｎｉａ | 多孔質支持体上の細胞への大きなカーゴの効率的な送達 |
| CN106795555B (zh) * | 2014-09-02 | 2021-04-02 | 谢达斌 | 聚合酶连锁反应的仪器与方法 |
| JP6779217B2 (ja) | 2014-10-24 | 2020-11-04 | アヴェクタス リミテッド | 細胞原形質膜を越える送達方法 |
| KR101702328B1 (ko) * | 2014-10-24 | 2017-02-03 | 명지대학교 산학협력단 | 살균처리용 나노 광열소재 어레이와 이의 제조방법, 이를 이용한 살균처리 시스템 및 살균처리 방법 |
| EP3037515A1 (en) * | 2014-12-28 | 2016-06-29 | Femtofab Co., Ltd. | Modified cell prepared by putting material into the cell without using delivery vehicle |
| EP3037518B1 (en) | 2014-12-28 | 2020-05-06 | Femtobiomed Inc. | Device for putting material into cell |
| US20180010149A1 (en) * | 2015-02-06 | 2018-01-11 | President And Fellows Of Harvard College | Plasmonic nanocavity-based cell therapy method and system |
| JP6680993B2 (ja) * | 2015-03-16 | 2020-04-15 | 株式会社ニコン | ピペットホルダ、マイクロマニピュレータ、及びマイクロインジェクションシステム |
| JP6090891B1 (ja) * | 2015-06-01 | 2017-03-08 | 株式会社片岡製作所 | 細胞処理方法、レーザ加工機、細胞培養容器 |
| JP2016225573A (ja) * | 2015-06-03 | 2016-12-28 | 株式会社東芝 | 基板処理装置および基板処理方法 |
| WO2017015063A1 (en) * | 2015-07-23 | 2017-01-26 | The Regents Of The University Of California | Plasmonic micropillar array with embedded nanoparticles for large area cell force sensing |
| US10134801B2 (en) * | 2015-11-30 | 2018-11-20 | Taiwan Semiconductor Manufacturing Company Limited | Method of forming deep trench isolation in radiation sensing substrate and image sensor device |
| CN105420278A (zh) * | 2015-12-09 | 2016-03-23 | 苏州大学 | 采用光致穿孔的方式制备负载有外源分子细胞的方法及制备该细胞用的基材及该细胞 |
| WO2017115128A2 (en) | 2015-12-30 | 2017-07-06 | Avectas Limited | Vector-free delivery of gene editing proteins and compositions to cells and tissues |
| CN106995783B (zh) * | 2016-01-22 | 2019-11-19 | 上海交通大学 | 细胞电转染装置及其应用 |
| WO2017155166A1 (ko) * | 2016-03-11 | 2017-09-14 | 가톨릭관동대학교기술지주 주식회사 | 물리적 자극에 의한 환경유입을 이용한 세포 리프로그래밍 방법 |
| KR101855967B1 (ko) * | 2016-03-11 | 2018-05-10 | 가톨릭관동대학교산학협력단 | 물리적 자극에 의한 환경유입을 이용한 세포 리프로그래밍 방법 |
| US10774302B2 (en) | 2016-03-29 | 2020-09-15 | Biosyntagma, Llc | Device and method for dissecting and analyzing individual cell samples |
| US9995686B2 (en) * | 2016-06-09 | 2018-06-12 | International Business Machines Corporation | Patch clamp technique with complementary Raman spectroscopy |
| EP3479102A1 (en) * | 2016-07-04 | 2019-05-08 | CellTool GmbH | Device and method for the determination of transfection |
| US10829729B2 (en) | 2016-11-03 | 2020-11-10 | President And Fellows Of Harvard College | Cellular poration using laser radiation |
| WO2018105748A1 (ja) * | 2016-12-09 | 2018-06-14 | 国立大学法人山口大学 | レーザーを用いた細胞内への外来物質の導入方法 |
| US20210032589A1 (en) * | 2018-02-12 | 2021-02-04 | Flagship Pioneering Innovations V, Inc. | Methods and devices for the isolation of subcellular components |
| CN108531396B (zh) * | 2018-03-30 | 2021-07-27 | 东南大学 | 一种用于细胞转染的微流控芯片 |
| EP3813908A4 (en) * | 2018-06-28 | 2022-04-20 | Rand, Kinneret | NON-CLOGGING AND ANTI-ADHESIVE MICROCAPILLARY NEEDLE WITH IMPROVED TIP VISIBILITY |
| CN109164121B (zh) * | 2018-08-01 | 2020-10-16 | 华东师范大学 | 用于透射电子显微镜表征的自组装原位液体腔的制备方法 |
| CN109507174B (zh) * | 2019-01-16 | 2020-12-29 | 济南大学 | 基于姜黄素复合ZnO纳米粒子猝灭鲁米诺电化学发光传感器的制备 |
| US11685891B2 (en) * | 2019-05-17 | 2023-06-27 | Regents Of The University Of Colorado | Precise mechanical disruption for intracellular delivery to cells and small organisms |
| EP3976159A4 (en) * | 2019-05-30 | 2023-10-11 | Indian Institute Of Science | CONTROLLING THE MOVEMENT OF MAGNETICALLY CONTROLLED MICROSCOPIC PARTICLES |
| US11617541B2 (en) | 2019-06-20 | 2023-04-04 | Cilag Gmbh International | Optical fiber waveguide in an endoscopic system for fluorescence imaging |
| US11122968B2 (en) | 2019-06-20 | 2021-09-21 | Cilag Gmbh International | Optical fiber waveguide in an endoscopic system for hyperspectral imaging |
| US12337317B2 (en) | 2019-07-25 | 2025-06-24 | Hewlett-Packard Development Company, L.P. | Cell poration and transfection apparatuses |
| CN110673662B (zh) * | 2019-09-04 | 2022-06-14 | 广东工业大学 | 一种精确控制药物分子的装置及方法 |
| CA3154775A1 (en) * | 2019-09-23 | 2021-04-01 | Trince bv | Method to increase the permeability of the plasma membrane of cells and a structure suitable for use in such method |
| CN110835603A (zh) * | 2019-10-09 | 2020-02-25 | 遵义医科大学珠海校区 | 一种快速实现细胞膜可逆性损伤的装置及方法 |
| EP4070784A4 (en) * | 2019-12-06 | 2023-01-18 | Biomodi Biotech (Suzhou) Co., Ltd | COMPOSITE MATERIAL, METHOD OF MANUFACTURE THEREOF AND USE THEREOF |
| KR102448973B1 (ko) * | 2020-02-14 | 2022-09-29 | 부산대학교 산학협력단 | 핫 스팟 형성 및 하이브리드 가열을 통해 마이크로웨이브 에너지 효율을 향상시키는 탄소 나노 튜브로 코팅된 마이크로웨이브 용기 및 그의 제조 방법 |
| CN113293137B (zh) * | 2020-02-21 | 2024-03-22 | 百脉迪生物科技(苏州)有限公司 | 一种基于细胞膜表面改性技术的树突状细胞的改性方法及其应用 |
| US20230174908A1 (en) * | 2020-04-30 | 2023-06-08 | Hewlett-Packard Development Company, L.P. | Reagent injections into cells |
| WO2022018760A1 (en) * | 2020-07-23 | 2022-01-27 | INDIAN INSTITUTE OF TECHNOLOGY MADRAS (IIT Madras) | Massively parallel high throughput single-cell optoporation |
| CN114105103B (zh) * | 2020-08-31 | 2023-04-07 | 中国科学院宁波材料技术与工程研究所慈溪生物医学工程研究所 | 一种复合变价金属氧化物粒子作为表面增强拉曼光谱基底的应用 |
| WO2022065460A1 (ja) * | 2020-09-24 | 2022-03-31 | 株式会社ニコン | 生物体の操作方法および操作装置 |
| CN112924436B (zh) * | 2021-01-29 | 2022-07-05 | 山东师范大学 | 一种银包裹的碗状二硫化钼复合金纳米颗粒sers基底及其制备方法和应用 |
| EP4312840A1 (en) * | 2021-03-23 | 2024-02-07 | Universiteit Gent | Method to selectively permeabilize and/or fragmentize cells |
| DE102021122999A1 (de) | 2021-09-06 | 2023-03-09 | Dionex Softron Gmbh | Nadel für die Verwendung in analytischen Anwendungen |
| CN113967487B (zh) * | 2021-10-11 | 2022-12-02 | 华中科技大学 | 一种喷嘴、液滴光热操控系统及其应用 |
| CN114621979B (zh) * | 2022-02-15 | 2024-04-09 | 北京大学 | 基于柔性可变截面微通道的细胞力学转染的方法和装置 |
| WO2024076719A1 (en) * | 2022-10-07 | 2024-04-11 | Board Of Regents For The Oklahoma Agricultural And Mechanical Colleges | Method for making multimetallic nanoparticles |
| WO2024243514A1 (en) * | 2023-05-25 | 2024-11-28 | Trustees Of Dartmouth College | Leveraging photothermal heating for surface processing of active agents |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009017695A1 (en) * | 2007-07-26 | 2009-02-05 | The Regents Of The University Of California | A single cell surgery tool and a cell transfection device utilizing the photothermal properties of thin films and/or metal nanoparticles |
Family Cites Families (64)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2178555A5 (enExample) | 1972-12-21 | 1973-11-09 | Petroles Cie Francaise | |
| US4469863A (en) | 1980-11-12 | 1984-09-04 | Ts O Paul O P | Nonionic nucleic acid alkyl and aryl phosphonates and processes for manufacture and use thereof |
| US5310674A (en) * | 1982-05-10 | 1994-05-10 | Bar-Ilan University | Apertured cell carrier |
| US5235033A (en) | 1985-03-15 | 1993-08-10 | Anti-Gene Development Group | Alpha-morpholino ribonucleoside derivatives and polymers thereof |
| US5034506A (en) | 1985-03-15 | 1991-07-23 | Anti-Gene Development Group | Uncharged morpholino-based polymers having achiral intersubunit linkages |
| JPH01141582A (ja) * | 1987-11-27 | 1989-06-02 | Shimadzu Corp | 遺伝子導入装置 |
| US5216141A (en) | 1988-06-06 | 1993-06-01 | Benner Steven A | Oligonucleotide analogs containing sulfur linkages |
| US4917462A (en) | 1988-06-15 | 1990-04-17 | Cornell Research Foundation, Inc. | Near field scanning optical microscopy |
| US5602240A (en) | 1990-07-27 | 1997-02-11 | Ciba Geigy Ag. | Backbone modified oligonucleotide analogs |
| US5386023A (en) | 1990-07-27 | 1995-01-31 | Isis Pharmaceuticals | Backbone modified oligonucleotide analogs and preparation thereof through reductive coupling |
| US5080586A (en) | 1990-09-24 | 1992-01-14 | Osada Research Institute, Ltd. | Apical foramen position detector for use in dental treatment |
| US5644048A (en) | 1992-01-10 | 1997-07-01 | Isis Pharmaceuticals, Inc. | Process for preparing phosphorothioate oligonucleotides |
| US5637684A (en) | 1994-02-23 | 1997-06-10 | Isis Pharmaceuticals, Inc. | Phosphoramidate and phosphorothioamidate oligomeric compounds |
| US5472749A (en) | 1994-10-27 | 1995-12-05 | Northwestern University | Graphite encapsulated nanophase particles produced by a tungsten arc method |
| JP2783984B2 (ja) | 1995-04-21 | 1998-08-06 | プリマハム株式会社 | マイクロマニピュレーション装置及びそれを用いた細胞操作方法 |
| US5958348A (en) | 1997-02-28 | 1999-09-28 | Nanogram Corporation | Efficient production of particles by chemical reaction |
| US6225007B1 (en) | 1999-02-05 | 2001-05-01 | Nanogram Corporation | Medal vanadium oxide particles |
| US5938979A (en) | 1997-10-31 | 1999-08-17 | Nanogram Corporation | Electromagnetic shielding |
| US20050019921A1 (en) | 2001-11-27 | 2005-01-27 | Orwar Owe E. | Method for combined sequential agent delivery and electroporation for cell structures and use thereof |
| ATE375752T1 (de) | 1998-03-06 | 2007-11-15 | Spectrx Inc | Integrierte gewebeporations-, flüssigkeitsammel- und analysevorrichtung |
| WO1999046588A1 (en) | 1998-03-12 | 1999-09-16 | Isao Karube | Apparatus for automatically measuring minute membrane potential |
| JP4467793B2 (ja) | 1998-03-12 | 2010-05-26 | 株式会社東京大学Ｔｌｏ | 細胞の特定部位穿孔技術 |
| US6200674B1 (en) | 1998-03-13 | 2001-03-13 | Nanogram Corporation | Tin oxide particles |
| JP3035608B2 (ja) | 1998-07-09 | 2000-04-24 | 農林水産省食品総合研究所長 | マイクロキャピラリーアレイ、その製造方法、及び物質注入装置 |
| US6468657B1 (en) | 1998-12-04 | 2002-10-22 | The Regents Of The University Of California | Controllable ion-exchange membranes |
| DE19905571C1 (de) * | 1999-02-11 | 2000-11-16 | Bosch Gmbh Robert | Verfahren zur Erzeugung definiert konischer Löcher mittels eines Laserstrahls |
| US6555781B2 (en) * | 1999-05-10 | 2003-04-29 | Nanyang Technological University | Ultrashort pulsed laser micromachining/submicromachining using an acoustooptic scanning device with dispersion compensation |
| US6300108B1 (en) | 1999-07-21 | 2001-10-09 | The Regents Of The University Of California | Controlled electroporation and mass transfer across cell membranes |
| US6485858B1 (en) | 1999-08-23 | 2002-11-26 | Catalytic Materials | Graphite nanofiber catalyst systems for use in fuel cell electrodes |
| US6254928B1 (en) | 1999-09-02 | 2001-07-03 | Micron Technology, Inc. | Laser pyrolysis particle forming method and particle forming method |
| AU2002239581B2 (en) | 2000-12-15 | 2006-09-28 | The Arizona Board Of Regents On Behalf Of The University Of Arizona | Method for patterning metal using nanoparticle containing precursors |
| US20020099356A1 (en) | 2001-01-19 | 2002-07-25 | Unger Evan C. | Transmembrane transport apparatus and method |
| US6706248B2 (en) | 2001-03-19 | 2004-03-16 | General Electric Company | Carbon nitrogen nanofiber compositions of specific morphology, and method for their preparation |
| US6555161B1 (en) | 2001-05-18 | 2003-04-29 | Ensci Inc. | Process for producing thin film metal oxide coated substrates |
| EP1440458B1 (en) | 2001-10-02 | 2007-01-10 | Invitrogen Corporation | Method of semiconductor nanoparticle synthesis |
| US6688494B2 (en) | 2001-12-20 | 2004-02-10 | Cima Nanotech, Inc. | Process for the manufacture of metal nanoparticle |
| AU2003233458A1 (en) | 2002-03-29 | 2003-10-13 | Array Bioscience Corporation | Methods for manufacturing nanoparticle structures using hydrophobic or charged surfaces |
| US6962685B2 (en) | 2002-04-17 | 2005-11-08 | International Business Machines Corporation | Synthesis of magnetite nanoparticles and the process of forming Fe-based nanomaterials |
| JP5010793B2 (ja) | 2002-07-09 | 2012-08-29 | 独立行政法人科学技術振興機構 | 電気注入法を用いた動物細胞への細胞内導入物質の導入方法及びその装置 |
| JP3703479B2 (ja) | 2002-09-20 | 2005-10-05 | 松下電器産業株式会社 | ナノ粒子の製造方法及び該製造方法によって製造されたナノ粒子 |
| US20040101822A1 (en) | 2002-11-26 | 2004-05-27 | Ulrich Wiesner | Fluorescent silica-based nanoparticles |
| EP1615988A2 (en) | 2003-01-10 | 2006-01-18 | Reveo, Inc. | Highly controllable electroporation and applications thereof |
| US6972046B2 (en) | 2003-01-13 | 2005-12-06 | International Business Machines Corporation | Process of forming magnetic nanocomposites via nanoparticle self-assembly |
| US20060047254A1 (en) | 2003-04-04 | 2006-03-02 | Ravi Nallakrishnan | Phacoemulsification needle |
| US20050118102A1 (en) | 2003-04-28 | 2005-06-02 | Intematix Corporation | Spin resonance heating and/or imaging in medical applications |
| US7060121B2 (en) | 2003-06-25 | 2006-06-13 | Hsing Kuang Lin | Method of producing gold nanoparticle |
| US7306823B2 (en) | 2004-09-18 | 2007-12-11 | Nanosolar, Inc. | Coated nanoparticles and quantum dots for solution-based fabrication of photovoltaic cells |
| US7212284B2 (en) | 2004-05-12 | 2007-05-01 | General Electric Company | Method for forming nanoparticle films and application thereof |
| US7824620B2 (en) | 2004-09-21 | 2010-11-02 | The Trustees Of The University Of Pennsylvania | Nano- and micro-scale structures: methods, devices and applications thereof |
| JP2006149227A (ja) * | 2004-11-25 | 2006-06-15 | Fujitsu Ltd | 微小物体の捕獲装置及び方法 |
| WO2006116021A2 (en) | 2005-04-22 | 2006-11-02 | Intematix Corporation | Mri technique based on electron spin resonance and endohedral contrast agent |
| US7666494B2 (en) | 2005-05-04 | 2010-02-23 | 3M Innovative Properties Company | Microporous article having metallic nanoparticle coating |
| GB0512216D0 (en) | 2005-06-15 | 2005-07-27 | Capsant Neurotechnologies Ltd | Device |
| US20110182805A1 (en) | 2005-06-17 | 2011-07-28 | Desimone Joseph M | Nanoparticle fabrication methods, systems, and materials |
| ES2865180T3 (es) | 2005-07-07 | 2021-10-15 | Univ California | Aparato para formación de cultivo celular |
| WO2007142674A2 (en) | 2005-10-27 | 2007-12-13 | Massachusetts Institute Of Technology | Nanoparticle heating and applications thereof |
| AU2007333220A1 (en) * | 2006-12-08 | 2008-06-19 | The Regents Of The University Of California | Remotely triggered release from heatable surfaces |
| WO2008127743A2 (en) * | 2007-01-05 | 2008-10-23 | William Marsh Rice University | Composition for targeted drug delivery and controlled release |
| JP2008238184A (ja) * | 2007-03-26 | 2008-10-09 | Mitsubishi Electric Corp | レーザ加工装置 |
| JP5205798B2 (ja) * | 2007-04-27 | 2013-06-05 | 富士通株式会社 | マイクロインジェクション装置、捕捉プレート、およびマイクロインジェクション方法 |
| US20120245042A1 (en) | 2011-03-14 | 2012-09-27 | The Trustees Of The University Of Pennsylvania | Debubbler for microfluidic systems |
| EP2707474B1 (en) | 2011-05-13 | 2019-07-10 | The Regents of The University of California | Photothermal substrates for selective transfection of cells |
| WO2014151888A1 (en) | 2013-03-15 | 2014-09-25 | The Regents Of The University Of California | High-throughput cargo delivery into live cells using photothermal platforms |
| JP6839073B2 (ja) | 2014-03-28 | 2021-03-03 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニアＴｈｅ Ｒｅｇｅｎｔｓ Ｏｆ Ｔｈｅ Ｕｎｉｖｅｒｓｉｔｙ Ｏｆ Ｃａｌｉｆｏｒｎｉａ | 多孔質支持体上の細胞への大きなカーゴの効率的な送達 |
-
2012
- 2012-05-14 EP EP12785188.9A patent/EP2707474B1/en active Active
- 2012-05-14 AU AU2012255988A patent/AU2012255988B2/en not_active Ceased
- 2012-05-14 CN CN201280034358.5A patent/CN103649295B/zh not_active Expired - Fee Related
- 2012-05-14 JP JP2014510543A patent/JP6144255B2/ja not_active Expired - Fee Related
- 2012-05-14 CA CA2873204A patent/CA2873204C/en active Active
- 2012-05-14 KR KR1020137033131A patent/KR101968778B1/ko not_active Expired - Fee Related
- 2012-05-14 US US14/117,543 patent/US20150044751A1/en not_active Abandoned
- 2012-05-14 WO PCT/US2012/037810 patent/WO2012158631A2/en not_active Ceased
- 2012-05-14 CN CN201510509151.4A patent/CN105132284B/zh not_active Expired - Fee Related
-
2014
- 2014-10-24 US US14/523,254 patent/US10435661B2/en active Active
-
2017
- 2017-05-10 JP JP2017093726A patent/JP6578319B2/ja not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009017695A1 (en) * | 2007-07-26 | 2009-02-05 | The Regents Of The University Of California | A single cell surgery tool and a cell transfection device utilizing the photothermal properties of thin films and/or metal nanoparticles |
Non-Patent Citations (2)
| Title |
|---|
| PARKER, E.R. et al., 'Bulk Titanium Microfluidic Networks for Protein Self-Assembly Studies', 13 July 2010, [retrieved 22 June 2016 from <URL:http://www.engineering.ucsb.edu/~memsucsb/Research/publications/parker_micro] * |
| WU, T. et al., 'Photothermal Nanoblade for Patterned Cell Membrane Cutting', Optics Express, 25 October 2010, Vol. 18(22), Page 23153-23160 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CA2873204A1 (en) | 2012-11-22 |
| CA2873204C (en) | 2021-10-19 |
| US20150197720A1 (en) | 2015-07-16 |
| WO2012158631A2 (en) | 2012-11-22 |
| EP2707474B1 (en) | 2019-07-10 |
| KR101968778B1 (ko) | 2019-04-12 |
| JP2017158580A (ja) | 2017-09-14 |
| JP6578319B2 (ja) | 2019-09-18 |
| CN105132284A (zh) | 2015-12-09 |
| US20150044751A1 (en) | 2015-02-12 |
| KR20140031943A (ko) | 2014-03-13 |
| WO2012158631A3 (en) | 2013-01-10 |
| EP2707474A2 (en) | 2014-03-19 |
| CN105132284B (zh) | 2018-04-03 |
| CN103649295A (zh) | 2014-03-19 |
| AU2012255988A1 (en) | 2014-01-09 |
| JP6144255B2 (ja) | 2017-06-07 |
| US10435661B2 (en) | 2019-10-08 |
| EP2707474A4 (en) | 2014-12-10 |
| CN103649295B (zh) | 2015-09-16 |
| JP2014513984A (ja) | 2014-06-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU2012255988B2 (en) | Photothermal substrates for selective transfection of cells | |
| US12391949B2 (en) | High-throughput cargo delivery into live cells using photothermal platforms | |
| US20110117648A1 (en) | Single cell surgery tool and a cell transfection device utilizing the photothermal properties of thin films and/or metal nanoparticles | |
| Stewart et al. | Intracellular delivery by membrane disruption: mechanisms, strategies, and concepts | |
| Wu et al. | Massively parallel delivery of large cargo into mammalian cells with light pulses | |
| Stachowiak et al. | Inkjet formation of unilamellar lipid vesicles for cell-like encapsulation | |
| Wu et al. | Photothermal nanoblade for large cargo delivery into mammalian cells | |
| US11999966B2 (en) | Apparatuses and methods using nanostraws to deliver biologically relevant cargo into non-adherent cells | |
| JP2005287419A (ja) | レーザインジェクション方法および装置 | |
| US20160083687A1 (en) | Method and device | |
| Wu et al. | Photothermal nanoblade for single cell surgery and large cargo delivery | |
| Wu et al. | Photothermal nanoblade for single cell surgery | |
| Mthunzi-Kufa | Optical sorting and photo-transfection of mammalian cells | |
| Wu | Patterned Photothermal Cavitation for Cell Surgeries |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FGA | Letters patent sealed or granted (standard patent) | ||
| MK14 | Patent ceased section 143(a) (annual fees not paid) or expired |