AU2009264336A1 - Methods for preparing human skin substitutes from human pluripotent stem cells - Google Patents

Methods for preparing human skin substitutes from human pluripotent stem cells Download PDF

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Publication number
AU2009264336A1
AU2009264336A1 AU2009264336A AU2009264336A AU2009264336A1 AU 2009264336 A1 AU2009264336 A1 AU 2009264336A1 AU 2009264336 A AU2009264336 A AU 2009264336A AU 2009264336 A AU2009264336 A AU 2009264336A AU 2009264336 A1 AU2009264336 A1 AU 2009264336A1
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Prior art keywords
human
cells
stem cells
pluripotent stem
keratinocytes
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Abandoned
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AU2009264336A
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Inventor
Christine Baldeschi
Hind Guenou
Gilles Lemaitre
Marc Peschanski
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Institut National de la Sante et de la Recherche Medicale INSERM
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Institut National de la Sante et de la Recherche Medicale INSERM
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Abandoned legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0603Embryonic cells ; Embryoid bodies
    • C12N5/0606Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • CCHEMISTRY; METALLURGY
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0625Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0629Keratinocytes; Whole skin
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61FFILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
    • A61F2/00Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
    • A61F2/02Prostheses implantable into the body
    • A61F2/10Hair or skin implants
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/36Skin; Hair; Nails; Sebaceous glands; Cerumen; Epidermis; Epithelial cells; Keratinocytes; Langerhans cells; Ectodermal cells
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00Drugs for dermatological disorders
    • A61P17/02Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
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    • C12N2500/30Organic components
    • C12N2500/40Nucleotides, nucleosides or bases
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    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/01Modulators of cAMP or cGMP, e.g. non-hydrolysable analogs, phosphodiesterase inhibitors, cholera toxin
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    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/11Epidermal growth factor [EGF]
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    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/155Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
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    • C12N2502/00Coculture with; Conditioned medium produced by
    • C12N2502/13Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
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    • C12N2506/00Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
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    • C12N2506/00Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells

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  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Cell Biology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Dermatology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Transplantation (AREA)
  • Developmental Biology & Embryology (AREA)
  • Reproductive Health (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Botany (AREA)
  • Vascular Medicine (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Cardiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AU2009264336A 2008-06-25 2009-06-23 Methods for preparing human skin substitutes from human pluripotent stem cells Abandoned AU2009264336A1 (en)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
EP08305320 2008-06-25
EP08305320.7 2008-06-25
EP08305447 2008-08-04
EP08305447.8 2008-08-04
US9095708P 2008-08-22 2008-08-22
US61/090,957 2008-08-22
EP09305077.1 2009-01-28
EP09305077 2009-01-28
PCT/EP2009/057817 WO2009156398A1 (en) 2008-06-25 2009-06-23 Methods for preparing human skin substitutes from human pluripotent stem cells

Publications (1)

Publication Number Publication Date
AU2009264336A1 true AU2009264336A1 (en) 2009-12-30

Family

ID=40863382

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2009264336A Abandoned AU2009264336A1 (en) 2008-06-25 2009-06-23 Methods for preparing human skin substitutes from human pluripotent stem cells

Country Status (8)

Country Link
US (1) US20110165130A1 (enExample)
EP (1) EP2297299A1 (enExample)
JP (1) JP2011525370A (enExample)
KR (1) KR20110022713A (enExample)
CN (1) CN102186969A (enExample)
AU (1) AU2009264336A1 (enExample)
BR (1) BRPI0914422A2 (enExample)
WO (1) WO2009156398A1 (enExample)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010134619A1 (ja) * 2009-05-18 2010-11-25 国立大学法人東北大学 人工多能性幹細胞からの上皮系前駆細胞・幹細胞群及び角膜上皮細胞群の分化誘導方法
US8529883B2 (en) 2010-05-07 2013-09-10 Fibrocell Technologies, Inc. Dosage unit formulations of autologous dermal fibroblasts
RU2458983C1 (ru) * 2011-07-18 2012-08-20 Российская Федерация, От Имени Которой Выступает Министерство Образования И Науки Российской Федерации Способ получения индуцированных плюрипотентных стволовых клеток из фибробластов пациентов с болезнью хангингтона
US20160237400A1 (en) * 2013-03-15 2016-08-18 The Jackson Laboratory Isolation of non-embryonic stem cells and uses thereof
EP2970892A1 (en) * 2013-03-15 2016-01-20 The Jackson Laboratory Isolation of non-embryonic stem cells and uses thereof
US9545302B2 (en) 2013-11-20 2017-01-17 Dermagenesis Llc Skin printing and auto-grafting
CN104789521B (zh) * 2014-01-22 2017-11-07 广州康睿生物医药科技股份有限公司 一种晶状体上皮干细胞的分离培养方法
KR102354410B1 (ko) * 2014-02-21 2022-01-21 다이니뽄 스미토모 세이야쿠 가부시키가이샤 눈 질환 치료제, 이의 스크리닝 방법, 및 망막 색소 상피 세포 이식과 연관된 거부 반응의 예측 방법
US20180230436A1 (en) * 2014-10-10 2018-08-16 Garrett VYGANTAS Methods of manufacturing keratin products and uses thereof
US10167451B2 (en) 2014-12-22 2019-01-01 The Chinese University Of Hong Kong Combinational use of mechanical manipulation and programin derivatives to increase Oct4, Sox2, or Nanog expression in fibroblasts
JP6736253B2 (ja) * 2014-12-22 2020-08-05 ザ チャイニーズ ユニバーシティ オブ ホンコン 体細胞から多能性幹細胞を作製するための機械的操作とプログラミンの併用
GB201510913D0 (en) * 2015-06-22 2015-08-05 Nat University Of Singapore And Agency For Science Technology And Res Vascularized tissue, skin or mucosa quivalent
US20190093072A1 (en) 2015-10-21 2019-03-28 Indiana University Research And Technology Corporation Methods of generating human inner ear sensory epithelia and sensory neurons
JP7018875B2 (ja) * 2015-10-21 2022-02-14 インディアナ ユニバーシティー リサーチ アンド テクノロジー コーポレーション 多能性幹細胞からのヒト皮膚オルガノイドの誘導
US10273549B2 (en) 2016-04-21 2019-04-30 Vitrolabs Inc. Engineered skin equivalent, method of manufacture thereof and products derived therefrom
CN110291188B (zh) * 2016-12-09 2025-01-03 芝加哥大学 组织类器官
CN106754652B (zh) * 2017-03-06 2019-04-02 广州润虹医药科技股份有限公司 iPS细胞分化成外胚层祖细胞的无血清诱导培养基及诱导方法
CN106834214B (zh) * 2017-03-27 2020-02-14 广州润虹医药科技股份有限公司 一种诱导多能干细胞形成角质形成细胞的诱导培养基及诱导方法
JP6916318B2 (ja) * 2017-06-30 2021-08-11 ステムラボ・インコーポレイテッド 尿細胞からケラチノサイト幹細胞への直接逆分化方法および逆分化したケラチノサイト幹細胞を利用した皮膚再生促進用組成物の製造方法
CN107988147B (zh) * 2017-12-19 2021-11-09 江汉大学 基于器官芯片与诱导多能干细胞的定向分化用于3d拟表皮构建的方法
CN110093306B (zh) * 2018-01-31 2022-12-02 中山大学 人工毛囊及其制备方法和应用
EP3875581A1 (en) 2020-03-02 2021-09-08 Centre d'Etude des Cellules Souches (CECS) Automated method for preparing keratinocytes
EP3875580A1 (en) 2020-03-02 2021-09-08 Centre d'Etude des Cellules Souches (CECS) Methods for preparing keratinocytes
EP3875124A1 (fr) 2020-03-02 2021-09-08 Urgo Recherche Innovation Et Developpement Procede d'obtention d'un tissu dermo-epidermique pre-vascularise
CN112941015B (zh) * 2021-03-19 2022-03-18 上海爱萨尔生物科技有限公司 一种基于多能干细胞分化制备角质细胞的添加剂及方法
CN113046300B (zh) * 2021-03-19 2024-08-06 上海爱萨尔生物科技有限公司 一种基于多能干细胞分化制备角质细胞的培养方法
CN112980775B (zh) * 2021-03-19 2024-01-23 上海爱萨尔生物科技有限公司 一种基于多能干细胞分化制备角质细胞的培养液
JP7315184B2 (ja) 2022-02-16 2023-07-26 株式会社コーセー 多能性幹細胞から表皮角化細胞への分化誘導方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2825370A1 (fr) * 2001-05-31 2002-12-06 Inst Nat Sante Rech Med Keratinocytes obtenus a partir de cellules souches embryonnaires de mammiferes

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BRPI0914422A2 (pt) 2017-03-21
CN102186969A (zh) 2011-09-14
US20110165130A1 (en) 2011-07-07
KR20110022713A (ko) 2011-03-07
WO2009156398A1 (en) 2009-12-30
EP2297299A1 (en) 2011-03-23
JP2011525370A (ja) 2011-09-22

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