BRPI0914422A2 - métodos para preparar substitutos de pele humana a partir de células tronco pluripotentes - Google Patents

métodos para preparar substitutos de pele humana a partir de células tronco pluripotentes

Info

Publication number
BRPI0914422A2
BRPI0914422A2 BRPI0914422A BRPI0914422A BRPI0914422A2 BR PI0914422 A2 BRPI0914422 A2 BR PI0914422A2 BR PI0914422 A BRPI0914422 A BR PI0914422A BR PI0914422 A BRPI0914422 A BR PI0914422A BR PI0914422 A2 BRPI0914422 A2 BR PI0914422A2
Authority
BR
Brazil
Prior art keywords
methods
stem cells
pluripotent stem
human skin
skin substitutes
Prior art date
Application number
BRPI0914422A
Other languages
English (en)
Portuguese (pt)
Inventor
Baldeschi Christine
Lemaitre Gilles
Guenou Hind
Peschanski Marc
Original Assignee
Inserm (Institut Nat De La Sante Et De La Rech Medicale)
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inserm (Institut Nat De La Sante Et De La Rech Medicale) filed Critical Inserm (Institut Nat De La Sante Et De La Rech Medicale)
Publication of BRPI0914422A2 publication Critical patent/BRPI0914422A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0629—Keratinocytes; Whole skin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
    • A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
    • A61F2/02—Prostheses implantable into the body
    • A61F2/10—Hair or skin implants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/36—Skin; Hair; Nails; Sebaceous glands; Cerumen; Epidermis; Epithelial cells; Keratinocytes; Langerhans cells; Ectodermal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/40—Nucleotides, nucleosides or bases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/01—Modulators of cAMP or cGMP, e.g. non-hydrolysable analogs, phosphodiesterase inhibitors, cholera toxin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/33—Insulin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/395—Thyroid hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Dermatology (AREA)
  • Cell Biology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Transplantation (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Reproductive Health (AREA)
  • Gynecology & Obstetrics (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Epidemiology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Cardiology (AREA)
  • Virology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Vascular Medicine (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Botany (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)
BRPI0914422A 2008-06-25 2009-06-23 métodos para preparar substitutos de pele humana a partir de células tronco pluripotentes BRPI0914422A2 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP08305320 2008-06-25
EP08305447 2008-08-04
US9095708P 2008-08-22 2008-08-22
EP09305077 2009-01-28
PCT/EP2009/057817 WO2009156398A1 (en) 2008-06-25 2009-06-23 Methods for preparing human skin substitutes from human pluripotent stem cells

Publications (1)

Publication Number Publication Date
BRPI0914422A2 true BRPI0914422A2 (pt) 2017-03-21

Family

ID=40863382

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0914422A BRPI0914422A2 (pt) 2008-06-25 2009-06-23 métodos para preparar substitutos de pele humana a partir de células tronco pluripotentes

Country Status (8)

Country Link
US (1) US20110165130A1 (enExample)
EP (1) EP2297299A1 (enExample)
JP (1) JP2011525370A (enExample)
KR (1) KR20110022713A (enExample)
CN (1) CN102186969A (enExample)
AU (1) AU2009264336A1 (enExample)
BR (1) BRPI0914422A2 (enExample)
WO (1) WO2009156398A1 (enExample)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010134619A1 (ja) * 2009-05-18 2010-11-25 国立大学法人東北大学 人工多能性幹細胞からの上皮系前駆細胞・幹細胞群及び角膜上皮細胞群の分化誘導方法
US8529883B2 (en) * 2010-05-07 2013-09-10 Fibrocell Technologies, Inc. Dosage unit formulations of autologous dermal fibroblasts
RU2458983C1 (ru) * 2011-07-18 2012-08-20 Российская Федерация, От Имени Которой Выступает Министерство Образования И Науки Российской Федерации Способ получения индуцированных плюрипотентных стволовых клеток из фибробластов пациентов с болезнью хангингтона
AU2014239954B2 (en) * 2013-03-15 2020-07-16 The Jackson Laboratory Isolation of non-embryonic stem cells and uses thereof
US20160237400A1 (en) * 2013-03-15 2016-08-18 The Jackson Laboratory Isolation of non-embryonic stem cells and uses thereof
US9545302B2 (en) 2013-11-20 2017-01-17 Dermagenesis Llc Skin printing and auto-grafting
CN104789521B (zh) * 2014-01-22 2017-11-07 广州康睿生物医药科技股份有限公司 一种晶状体上皮干细胞的分离培养方法
SG10202103834VA (en) * 2014-02-21 2021-05-28 Sumitomo Dainippon Pharma Co Ltd Eye disease treatment agent, screening method therefor, and method for predicting rejection response associated with retinal pigment epithelial cell transplant
WO2016057833A1 (en) * 2014-10-10 2016-04-14 Vygantas Garrett Methods of manufacturing keratin products and uses thereof
JP6736253B2 (ja) * 2014-12-22 2020-08-05 ザ チャイニーズ ユニバーシティ オブ ホンコン 体細胞から多能性幹細胞を作製するための機械的操作とプログラミンの併用
US10167451B2 (en) 2014-12-22 2019-01-01 The Chinese University Of Hong Kong Combinational use of mechanical manipulation and programin derivatives to increase Oct4, Sox2, or Nanog expression in fibroblasts
GB201510913D0 (en) * 2015-06-22 2015-08-05 Nat University Of Singapore And Agency For Science Technology And Res Vascularized tissue, skin or mucosa quivalent
CA3002164A1 (en) * 2015-10-21 2017-04-27 Indiana University Research And Technology Corporation Derivation of human skin organoids from pluripotent stem cells
AU2016341982B2 (en) 2015-10-21 2022-07-07 Indiana University Research And Technology Corporation Methods of generating human inner ear sensory epithelia and sensory neurons
US10273549B2 (en) 2016-04-21 2019-04-30 Vitrolabs Inc. Engineered skin equivalent, method of manufacture thereof and products derived therefrom
EP3551746A1 (en) * 2016-12-09 2019-10-16 The University of Chicago Tissue organoids
CN106754652B (zh) * 2017-03-06 2019-04-02 广州润虹医药科技股份有限公司 iPS细胞分化成外胚层祖细胞的无血清诱导培养基及诱导方法
CN106834214B (zh) * 2017-03-27 2020-02-14 广州润虹医药科技股份有限公司 一种诱导多能干细胞形成角质形成细胞的诱导培养基及诱导方法
JP6916318B2 (ja) * 2017-06-30 2021-08-11 ステムラボ・インコーポレイテッド 尿細胞からケラチノサイト幹細胞への直接逆分化方法および逆分化したケラチノサイト幹細胞を利用した皮膚再生促進用組成物の製造方法
CN107988147B (zh) * 2017-12-19 2021-11-09 江汉大学 基于器官芯片与诱导多能干细胞的定向分化用于3d拟表皮构建的方法
CN110093306B (zh) * 2018-01-31 2022-12-02 中山大学 人工毛囊及其制备方法和应用
EP3875581A1 (en) 2020-03-02 2021-09-08 Centre d'Etude des Cellules Souches (CECS) Automated method for preparing keratinocytes
EP3875124A1 (fr) 2020-03-02 2021-09-08 Urgo Recherche Innovation Et Developpement Procede d'obtention d'un tissu dermo-epidermique pre-vascularise
EP3875580A1 (en) 2020-03-02 2021-09-08 Centre d'Etude des Cellules Souches (CECS) Methods for preparing keratinocytes
CN112941015B (zh) * 2021-03-19 2022-03-18 上海爱萨尔生物科技有限公司 一种基于多能干细胞分化制备角质细胞的添加剂及方法
CN112980775B (zh) * 2021-03-19 2024-01-23 上海爱萨尔生物科技有限公司 一种基于多能干细胞分化制备角质细胞的培养液
CN113046300B (zh) * 2021-03-19 2024-08-06 上海爱萨尔生物科技有限公司 一种基于多能干细胞分化制备角质细胞的培养方法
JP7315184B2 (ja) * 2022-02-16 2023-07-26 株式会社コーセー 多能性幹細胞から表皮角化細胞への分化誘導方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2825370A1 (fr) * 2001-05-31 2002-12-06 Inst Nat Sante Rech Med Keratinocytes obtenus a partir de cellules souches embryonnaires de mammiferes

Also Published As

Publication number Publication date
JP2011525370A (ja) 2011-09-22
KR20110022713A (ko) 2011-03-07
US20110165130A1 (en) 2011-07-07
EP2297299A1 (en) 2011-03-23
WO2009156398A1 (en) 2009-12-30
AU2009264336A1 (en) 2009-12-30
CN102186969A (zh) 2011-09-14

Similar Documents

Publication Publication Date Title
BRPI0914422A2 (pt) métodos para preparar substitutos de pele humana a partir de células tronco pluripotentes
BRPI1008297A2 (pt) Metodo para a criopreservacao de celulas, estruturas celulares artificiais ou montagens de tecidos complexos tridimensionais
EP2538988A4 (en) METHOD FOR TISSUE-DECELULARIZATION
IL245535B (en) Method of engrafting cells from solid tissues
EP2600779A4 (en) METHOD FOR PRODUCING A SKIN IMPLANT
EP2358297A4 (en) HEART LAPTOP PROSTHESIS AND METHOD
BR112013002645A2 (pt) dispositivo para obter um enxerto de pele
EP2349097C0 (en) IMPROVED ARTIFICIAL VALVE
BRPI1007353A2 (pt) tensor de pele para transplante de cabelo
BR112012000826A2 (pt) métodos para preparação de vesículas e formulações produzidas a partir dessas
BRPI1011127A2 (pt) métodos para o cultivo de células tronco e células progenitoras
FI20096288A0 (fi) Formulations and methods for culturing stem cells
ES2674239T8 (es) Medios para células madre pluripotentes carentes de alimentadores que contienen suero humano
ZA201206936B (en) Method of preparation of radiation-curable colored artificial nail gels
EP2389573A4 (en) SKIN ANALYSIS PROCEDURE
EP2499241A4 (en) PROCESS FOR PRODUCING NEURAL STEM CELLS
GB2497475B (en) Kits for tissue reconstruction
PT2552401T (pt) Preparação criada a partir de uma cultura in vitro de células não elicitadas, indiferenciadas da árvore argânia, uso da mesma para tratamento de envelhecimento da pele
ZA201106240B (en) Method and means for producing tissues and tissues obtained
EP2524033A4 (en) METHOD FOR SCREENING INDUCED PLURIPOTENTAL STEM CELLS
EP2021464A4 (en) METHOD FOR CULTURING HUMAN EMBRYONIC STEM CELLS
EP2543396A4 (en) ARTIFICIAL SKIN
EP2265708A4 (en) METHODS OF STEM CELL PRODUCTION AND THERAPY
HRP20181699T1 (hr) Postupak oblikovanja imitacija grana
BR112012001107A2 (pt) Células derivadas de tecido cardíaco

Legal Events

Date Code Title Description
B08F Application fees: application dismissed [chapter 8.6 patent gazette]
B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]