CN102186969A - 由人多能干细胞制备人皮肤替代品的方法 - Google Patents

由人多能干细胞制备人皮肤替代品的方法 Download PDF

Info

Publication number
CN102186969A
CN102186969A CN2009801256559A CN200980125655A CN102186969A CN 102186969 A CN102186969 A CN 102186969A CN 2009801256559 A CN2009801256559 A CN 2009801256559A CN 200980125655 A CN200980125655 A CN 200980125655A CN 102186969 A CN102186969 A CN 102186969A
Authority
CN
China
Prior art keywords
human
cells
stem cells
pluripotent stem
keratinocyte
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN2009801256559A
Other languages
English (en)
Chinese (zh)
Inventor
欣德·盖农
吉勒·勒迈特
克里斯蒂娜·巴尔代斯基
马克·佩先斯基
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
NATIONAL HEALTH AND MEDICINE INST
Original Assignee
NATIONAL HEALTH AND MEDICINE INST
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by NATIONAL HEALTH AND MEDICINE INST filed Critical NATIONAL HEALTH AND MEDICINE INST
Publication of CN102186969A publication Critical patent/CN102186969A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0629—Keratinocytes; Whole skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
    • A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
    • A61F2/02—Prostheses implantable into the body
    • A61F2/10—Hair or skin implants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/36—Skin; Hair; Nails; Sebaceous glands; Cerumen; Epidermis; Epithelial cells; Keratinocytes; Langerhans cells; Ectodermal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/40—Nucleotides, nucleosides or bases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/01—Modulators of cAMP or cGMP, e.g. non-hydrolysable analogs, phosphodiesterase inhibitors, cholera toxin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/33—Insulin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/395—Thyroid hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Dermatology (AREA)
  • Cell Biology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Transplantation (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Reproductive Health (AREA)
  • Gynecology & Obstetrics (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Epidemiology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Cardiology (AREA)
  • Virology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Vascular Medicine (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Botany (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)
CN2009801256559A 2008-06-25 2009-06-23 由人多能干细胞制备人皮肤替代品的方法 Pending CN102186969A (zh)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
EP08305320 2008-06-25
EP08305320.7 2008-06-25
EP08305447 2008-08-04
EP08305447.8 2008-08-04
US9095708P 2008-08-22 2008-08-22
US61/090,957 2008-08-22
EP09305077.1 2009-01-28
EP09305077 2009-01-28
PCT/EP2009/057817 WO2009156398A1 (en) 2008-06-25 2009-06-23 Methods for preparing human skin substitutes from human pluripotent stem cells

Publications (1)

Publication Number Publication Date
CN102186969A true CN102186969A (zh) 2011-09-14

Family

ID=40863382

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2009801256559A Pending CN102186969A (zh) 2008-06-25 2009-06-23 由人多能干细胞制备人皮肤替代品的方法

Country Status (8)

Country Link
US (1) US20110165130A1 (enExample)
EP (1) EP2297299A1 (enExample)
JP (1) JP2011525370A (enExample)
KR (1) KR20110022713A (enExample)
CN (1) CN102186969A (enExample)
AU (1) AU2009264336A1 (enExample)
BR (1) BRPI0914422A2 (enExample)
WO (1) WO2009156398A1 (enExample)

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104789521A (zh) * 2014-01-22 2015-07-22 广州康睿生物医药科技有限公司 一种晶状体上皮干细胞的分离培养方法
CN106754652A (zh) * 2017-03-06 2017-05-31 广州润虹医药科技有限公司 iPS细胞分化成外胚层祖细胞的无血清诱导培养基及诱导方法
CN106834214A (zh) * 2017-03-27 2017-06-13 广州润虹医药科技有限公司 一种诱导多能干细胞形成角质形成细胞的诱导培养基及诱导方法
CN107849530A (zh) * 2015-06-22 2018-03-27 新加坡国立大学 血管化组织、皮肤或黏膜等效物
CN107988147A (zh) * 2017-12-19 2018-05-04 江汉大学 基于器官芯片与诱导多能干细胞的定向分化用于3d拟表皮构建的方法
CN108367099A (zh) * 2015-10-21 2018-08-03 印第安纳大学研究与技术公司 来自多能干细胞的人皮肤类器官的衍生
CN110093306A (zh) * 2018-01-31 2019-08-06 中山大学 人工毛囊及其制备方法和应用
CN112941015A (zh) * 2021-03-19 2021-06-11 上海爱萨尔生物科技有限公司 一种基于多能干细胞分化制备角质细胞的添加剂及方法
CN112980775A (zh) * 2021-03-19 2021-06-18 上海爱萨尔生物科技有限公司 一种基于多能干细胞分化制备角质细胞的培养液
CN113046300A (zh) * 2021-03-19 2021-06-29 上海爱萨尔生物科技有限公司 一种基于多能干细胞分化制备角质细胞的培养方法
US12077778B2 (en) 2015-10-21 2024-09-03 Indiana University Research And Technology Corporation Methods of generating human inner ear sensory epithelia and sensory neurons

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010134619A1 (ja) * 2009-05-18 2010-11-25 国立大学法人東北大学 人工多能性幹細胞からの上皮系前駆細胞・幹細胞群及び角膜上皮細胞群の分化誘導方法
US8529883B2 (en) * 2010-05-07 2013-09-10 Fibrocell Technologies, Inc. Dosage unit formulations of autologous dermal fibroblasts
RU2458983C1 (ru) * 2011-07-18 2012-08-20 Российская Федерация, От Имени Которой Выступает Министерство Образования И Науки Российской Федерации Способ получения индуцированных плюрипотентных стволовых клеток из фибробластов пациентов с болезнью хангингтона
AU2014239954B2 (en) * 2013-03-15 2020-07-16 The Jackson Laboratory Isolation of non-embryonic stem cells and uses thereof
US20160237400A1 (en) * 2013-03-15 2016-08-18 The Jackson Laboratory Isolation of non-embryonic stem cells and uses thereof
US9545302B2 (en) 2013-11-20 2017-01-17 Dermagenesis Llc Skin printing and auto-grafting
SG10202103834VA (en) * 2014-02-21 2021-05-28 Sumitomo Dainippon Pharma Co Ltd Eye disease treatment agent, screening method therefor, and method for predicting rejection response associated with retinal pigment epithelial cell transplant
WO2016057833A1 (en) * 2014-10-10 2016-04-14 Vygantas Garrett Methods of manufacturing keratin products and uses thereof
JP6736253B2 (ja) * 2014-12-22 2020-08-05 ザ チャイニーズ ユニバーシティ オブ ホンコン 体細胞から多能性幹細胞を作製するための機械的操作とプログラミンの併用
US10167451B2 (en) 2014-12-22 2019-01-01 The Chinese University Of Hong Kong Combinational use of mechanical manipulation and programin derivatives to increase Oct4, Sox2, or Nanog expression in fibroblasts
US10273549B2 (en) 2016-04-21 2019-04-30 Vitrolabs Inc. Engineered skin equivalent, method of manufacture thereof and products derived therefrom
EP3551746A1 (en) * 2016-12-09 2019-10-16 The University of Chicago Tissue organoids
JP6916318B2 (ja) * 2017-06-30 2021-08-11 ステムラボ・インコーポレイテッド 尿細胞からケラチノサイト幹細胞への直接逆分化方法および逆分化したケラチノサイト幹細胞を利用した皮膚再生促進用組成物の製造方法
EP3875581A1 (en) 2020-03-02 2021-09-08 Centre d'Etude des Cellules Souches (CECS) Automated method for preparing keratinocytes
EP3875124A1 (fr) 2020-03-02 2021-09-08 Urgo Recherche Innovation Et Developpement Procede d'obtention d'un tissu dermo-epidermique pre-vascularise
EP3875580A1 (en) 2020-03-02 2021-09-08 Centre d'Etude des Cellules Souches (CECS) Methods for preparing keratinocytes
JP7315184B2 (ja) * 2022-02-16 2023-07-26 株式会社コーセー 多能性幹細胞から表皮角化細胞への分化誘導方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2825370A1 (fr) * 2001-05-31 2002-12-06 Inst Nat Sante Rech Med Keratinocytes obtenus a partir de cellules souches embryonnaires de mammiferes

Cited By (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104789521B (zh) * 2014-01-22 2017-11-07 广州康睿生物医药科技股份有限公司 一种晶状体上皮干细胞的分离培养方法
CN104789521A (zh) * 2014-01-22 2015-07-22 广州康睿生物医药科技有限公司 一种晶状体上皮干细胞的分离培养方法
CN107849530A (zh) * 2015-06-22 2018-03-27 新加坡国立大学 血管化组织、皮肤或黏膜等效物
CN108367099A (zh) * 2015-10-21 2018-08-03 印第安纳大学研究与技术公司 来自多能干细胞的人皮肤类器官的衍生
US12077778B2 (en) 2015-10-21 2024-09-03 Indiana University Research And Technology Corporation Methods of generating human inner ear sensory epithelia and sensory neurons
CN108367099B (zh) * 2015-10-21 2021-11-23 印第安纳大学研究与技术公司 来自多能干细胞的人皮肤类器官的衍生
CN106754652A (zh) * 2017-03-06 2017-05-31 广州润虹医药科技有限公司 iPS细胞分化成外胚层祖细胞的无血清诱导培养基及诱导方法
CN106754652B (zh) * 2017-03-06 2019-04-02 广州润虹医药科技股份有限公司 iPS细胞分化成外胚层祖细胞的无血清诱导培养基及诱导方法
CN106834214A (zh) * 2017-03-27 2017-06-13 广州润虹医药科技有限公司 一种诱导多能干细胞形成角质形成细胞的诱导培养基及诱导方法
CN106834214B (zh) * 2017-03-27 2020-02-14 广州润虹医药科技股份有限公司 一种诱导多能干细胞形成角质形成细胞的诱导培养基及诱导方法
CN107988147B (zh) * 2017-12-19 2021-11-09 江汉大学 基于器官芯片与诱导多能干细胞的定向分化用于3d拟表皮构建的方法
CN107988147A (zh) * 2017-12-19 2018-05-04 江汉大学 基于器官芯片与诱导多能干细胞的定向分化用于3d拟表皮构建的方法
CN110093306A (zh) * 2018-01-31 2019-08-06 中山大学 人工毛囊及其制备方法和应用
CN110093306B (zh) * 2018-01-31 2022-12-02 中山大学 人工毛囊及其制备方法和应用
CN113046300A (zh) * 2021-03-19 2021-06-29 上海爱萨尔生物科技有限公司 一种基于多能干细胞分化制备角质细胞的培养方法
CN112980775A (zh) * 2021-03-19 2021-06-18 上海爱萨尔生物科技有限公司 一种基于多能干细胞分化制备角质细胞的培养液
CN112980775B (zh) * 2021-03-19 2024-01-23 上海爱萨尔生物科技有限公司 一种基于多能干细胞分化制备角质细胞的培养液
CN112941015A (zh) * 2021-03-19 2021-06-11 上海爱萨尔生物科技有限公司 一种基于多能干细胞分化制备角质细胞的添加剂及方法

Also Published As

Publication number Publication date
BRPI0914422A2 (pt) 2017-03-21
JP2011525370A (ja) 2011-09-22
KR20110022713A (ko) 2011-03-07
US20110165130A1 (en) 2011-07-07
EP2297299A1 (en) 2011-03-23
WO2009156398A1 (en) 2009-12-30
AU2009264336A1 (en) 2009-12-30

Similar Documents

Publication Publication Date Title
CN102186969A (zh) 由人多能干细胞制备人皮肤替代品的方法
Tsuchiyama et al. Functional melanocytes are readily reprogrammable from multilineage-differentiating stress-enduring (muse) cells, distinct stem cells in human fibroblasts
US20190367883A1 (en) Regulating stem cells
JP2011525370A5 (enExample)
Aberdam Derivation of keratinocyte progenitor cells and skin formation from embryonic stem cells
WO2011104200A1 (en) Methods for preparing human melanocytes from human pluripotent stem cells
JP4550582B2 (ja) 毛髪誘導細胞の培養
CN106661547B (zh) 调控毛发生长的方法和组合物
Ji et al. Generation and differentiation of human embryonic stem cell-derived keratinocyte precursors
Ganjibakhsh et al. Three-dimensional decellularized amnion membrane scaffold promotes the efficiency of male germ cells generation from human induced pluripotent stem cells
JP2016516394A (ja) ヒト線維芽細胞において特徴的な幹細胞であるＭｕｓｅ細胞から容易に分化した機能性メラノサイトの使用
JP2015097524A (ja) 再構成皮膚の作製用の組成物および方法
WO2009080794A1 (en) Method for preparing cell-specific extracellular matrices
Heng et al. Directing stem cells into the keratinocyte lineage in vitro
Yonetani et al. In vitro expansion of immature melanoblasts and their ability to repopulate melanocyte stem cells in the hair follicle
KR101760239B1 (ko) 세포배양 삽입체를 이용한 인간 배아줄기세포 유래 중간엽 세포의 분리방법
Kinebuchi et al. Direct induction of layered tissues from mouse embryonic stem cells: potential for differentiation into urinary tract tissue
Dong et al. Enrichment of epidermal stem cells by rapid adherence and analysis of the reciprocal interaction of epidermal stem cells with neighboring cells using an organotypic system
US20230081733A1 (en) Methods for preparing keratinocytes
Sah et al. Human induced pluripotent stem cell–derived keratinocyte progenitors
A Rahman The development of in vitro models of human salivary glands
Dezawa et al. Regenerating Melanocytes: Current Stem Cell Approaches with Focus on Muse Cells
Shamis hESC-and iPSC-Derived Fibroblasts for Skin Engineering and Repair

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C02 Deemed withdrawal of patent application after publication (patent law 2001)
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20110914