AU2009203608B2 - Protein expression systems - Google Patents

Protein expression systems Download PDF

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Publication number
AU2009203608B2
AU2009203608B2 AU2009203608A AU2009203608A AU2009203608B2 AU 2009203608 B2 AU2009203608 B2 AU 2009203608B2 AU 2009203608 A AU2009203608 A AU 2009203608A AU 2009203608 A AU2009203608 A AU 2009203608A AU 2009203608 B2 AU2009203608 B2 AU 2009203608B2
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AU
Australia
Prior art keywords
rna
gene
sequence
protein
initiation site
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU2009203608A
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English (en)
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AU2009203608A1 (en
Inventor
George Peter Lomonossoff
Frank Sainsbury
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Plant Bioscience Ltd
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Plant Bioscience Ltd
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Application filed by Plant Bioscience Ltd filed Critical Plant Bioscience Ltd
Publication of AU2009203608A1 publication Critical patent/AU2009203608A1/en
Application granted granted Critical
Publication of AU2009203608B2 publication Critical patent/AU2009203608B2/en
Ceased legal-status Critical Current
Anticipated expiration legal-status Critical

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C07K14/08RNA viruses
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203Virus mediated transformation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C12N15/8258Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon for the production of oral vaccines (antigens) or immunoglobulins

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
AU2009203608A 2008-01-08 2009-01-08 Protein expression systems Ceased AU2009203608B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0800272.7 2008-01-08
GBGB0800272.7A GB0800272D0 (en) 2008-01-08 2008-01-08 Protein expression systems
PCT/GB2009/000060 WO2009087391A1 (en) 2008-01-08 2009-01-08 Protein expression systems

Publications (2)

Publication Number Publication Date
AU2009203608A1 AU2009203608A1 (en) 2009-07-16
AU2009203608B2 true AU2009203608B2 (en) 2014-03-27

Family

ID=39111256

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2009203608A Ceased AU2009203608B2 (en) 2008-01-08 2009-01-08 Protein expression systems

Country Status (18)

Country Link
US (5) US8674084B2 (https=)
EP (1) EP2240589B1 (https=)
JP (1) JP5745857B2 (https=)
KR (1) KR101602972B1 (https=)
CN (1) CN101952446B (https=)
AU (1) AU2009203608B2 (https=)
BR (1) BRPI0906509A2 (https=)
CA (1) CA2711895C (https=)
DK (1) DK2240589T3 (https=)
ES (1) ES2537198T3 (https=)
GB (1) GB0800272D0 (https=)
IL (1) IL206606A (https=)
MX (1) MX2010007336A (https=)
NZ (1) NZ586939A (https=)
PT (1) PT2240589E (https=)
RU (1) RU2539793C2 (https=)
WO (1) WO2009087391A1 (https=)
ZA (1) ZA201005245B (https=)

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CA2615372A1 (en) 2007-07-13 2009-01-13 Marc-Andre D'aoust Influenza virus-like particles (vlps) comprising hemagglutinin
WO2009076778A1 (en) 2007-11-27 2009-06-25 Medicago Inc. Recombinant influenza virus-like particles (vlps) produced in transgenic plants expressing hemagglutinin
GB0800272D0 (en) 2008-01-08 2008-02-13 Plant Bioscience Ltd Protein expression systems
AU2009267769B2 (en) 2008-07-08 2015-07-16 Medicago Inc. Soluble recombinant influenza antigens
HUE039100T2 (hu) 2009-06-24 2018-12-28 Medicago Inc Hemagglutinint tartalmazó kimer influenzavírus-jellegû részecskék
GB201002434D0 (en) * 2010-02-12 2010-03-31 Plant Bioscience Ltd Non-infectious RNA source
WO2012058762A1 (en) 2010-11-04 2012-05-10 Medicago Inc. Plant expression system
PT2665818T (pt) 2011-01-17 2017-07-05 Philip Morris Products Sa Expressão de proteínas em plantas
CA2824155A1 (en) 2011-01-17 2012-07-26 Philip Morris Products S.A. Vectors for nucleic acid expression in plants
US11390878B2 (en) 2011-09-30 2022-07-19 Medicago Inc. Increasing protein yield in plants
NZ622731A (en) 2011-09-30 2016-04-29 Medicago Inc Increasing virus-like particle yield in plants
BR112014011345A2 (pt) * 2011-11-11 2017-05-16 Philip Morris Products Sa partículas tipo vírus da influenza (vlps) compreendendo nicotiana tabacum produzida por hemaglutinina
JP2015528285A (ja) * 2012-09-05 2015-09-28 メディカゴ インコーポレイテッド 植物におけるピコルナウイルス様粒子の産生
KR101449155B1 (ko) * 2012-12-06 2014-10-13 주식회사 바이오앱 식물체에서 목적 단백질의 번역 효율을 증진시키기 위한 염기서열
US12467058B2 (en) 2013-03-28 2025-11-11 Aramis Biotechnologies Inc. Influenza virus-like particle production in plants
ES2803508T3 (es) 2013-03-28 2021-01-27 Medicago Inc Producción de partículas similares al virus de la gripe en plantas
RU2699982C2 (ru) * 2014-01-10 2019-09-11 Медикаго Инк. Энхансерные элементы cpmv
US10563213B2 (en) 2014-03-27 2020-02-18 Medicago Inc. Modified CPMV enhancer elements
RU2731295C2 (ru) 2014-07-11 2020-09-01 Медикаго Инк. Модификация продуцирования белков в растениях
AU2016208999A1 (en) 2015-01-23 2017-07-13 Medicago Inc. Rotavirus-like particle production in plants
JP2018524345A (ja) 2015-07-02 2018-08-30 メディカゴ インコーポレイテッド ジャスモン酸経路アクチベーター
CN110506118A (zh) * 2017-02-02 2019-11-26 杜克大学 用于控制基因表达的组合物和方法
GB201721600D0 (en) 2017-12-21 2018-02-07 Plant Bioscience Ltd Metabolic engineering
CN112400021A (zh) 2018-03-14 2021-02-23 麦迪卡格公司 植物表达增强子
GB201808617D0 (en) 2018-05-25 2018-07-11 Plant Bioscience Ltd Scaffold modification
WO2020033416A1 (en) * 2018-08-06 2020-02-13 Northwestern University Combined transcription and translation platform derived from plant chloroplasts
WO2020181354A1 (en) 2019-03-14 2020-09-17 Mitsubishi Tanabe Pharma Corporation Endogenous plant expression enhancer
GB201908431D0 (en) 2019-06-12 2019-07-24 Plant Bioscience Ltd Biosynthetic genes and polypeptides
CN114040979B (zh) * 2019-06-21 2024-06-28 国立大学法人大阪大学 稳定地保持外源基因的人工重组rna病毒的制作方法
GB201909104D0 (en) 2019-06-25 2019-08-07 Plant Bioscience Ltd Transferase enzymes
CN110468129B (zh) * 2019-08-08 2023-03-24 四川农业大学 一种基于cmv rna2的体外干扰黄瓜花叶病的制剂、其制备方法与应用
CN111777670B (zh) * 2020-06-19 2021-07-30 东北农业大学 一种pH调节自组装抗菌肽及制备方法和应用
WO2022260849A1 (en) 2021-06-09 2022-12-15 Nant Holdings Ip, Llc Methods and systems for producing a protein of interest in a plant
CN114181969B (zh) * 2021-12-09 2024-05-28 苏州大学 一种制备包裹外源蛋白的多角体的方法
GB202209501D0 (en) 2022-06-29 2022-08-10 Plant Bioscience Ltd Biosynthetic enzymes
CN121195070A (zh) 2023-04-03 2025-12-23 斯威夫特制药有限责任公司 用于在植物中高效异源基因表达的反式互补载体系统
KR20250082120A (ko) * 2023-11-29 2025-06-09 서강대학교산학협력단 검은눈콩 모자이크 바이러스의 바이러스 유사 입자의 제조방법 및 이의 활용

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US10563213B2 (en) 2014-03-27 2020-02-18 Medicago Inc. Modified CPMV enhancer elements

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Also Published As

Publication number Publication date
US20140250549A1 (en) 2014-09-04
US20200392522A1 (en) 2020-12-17
KR101602972B1 (ko) 2016-03-11
EP2240589B1 (en) 2015-03-04
MX2010007336A (es) 2010-08-16
CA2711895C (en) 2016-08-09
EP2240589A1 (en) 2010-10-20
RU2010133217A (ru) 2012-02-20
IL206606A (en) 2015-11-30
ES2537198T3 (es) 2015-06-03
BRPI0906509A2 (pt) 2015-12-01
CN101952446B (zh) 2014-06-11
AU2009203608A1 (en) 2009-07-16
CN101952446A (zh) 2011-01-19
US8674084B2 (en) 2014-03-18
JP5745857B2 (ja) 2015-07-08
JP2011509081A (ja) 2011-03-24
DK2240589T3 (en) 2015-05-04
US10822613B2 (en) 2020-11-03
IL206606A0 (en) 2010-12-30
KR20100109950A (ko) 2010-10-11
CA2711895A1 (en) 2009-07-16
US20210155947A1 (en) 2021-05-27
US10947549B2 (en) 2021-03-16
PT2240589E (pt) 2015-06-18
US20180030462A1 (en) 2018-02-01
NZ586939A (en) 2012-08-31
US20100287670A1 (en) 2010-11-11
ZA201005245B (en) 2012-01-25
RU2539793C2 (ru) 2015-01-27
WO2009087391A1 (en) 2009-07-16
GB0800272D0 (en) 2008-02-13

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