CA2711895C - Methods for protein production employing expression enhancer sequences derived from the rna-z segment of a comoviridae bipartite rna virus - Google Patents

Methods for protein production employing expression enhancer sequences derived from the rna-z segment of a comoviridae bipartite rna virus Download PDF

Info

Publication number
CA2711895C
CA2711895C CA2711895A CA2711895A CA2711895C CA 2711895 C CA2711895 C CA 2711895C CA 2711895 A CA2711895 A CA 2711895A CA 2711895 A CA2711895 A CA 2711895A CA 2711895 C CA2711895 C CA 2711895C
Authority
CA
Canada
Prior art keywords
rna
gene
sequence
initiation site
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2711895A
Other languages
English (en)
French (fr)
Other versions
CA2711895A1 (en
Inventor
George Peter Lomonossoff
Frank Sainsbury
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Plant Bioscience Ltd
Original Assignee
Plant Bioscience Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Plant Bioscience Ltd filed Critical Plant Bioscience Ltd
Publication of CA2711895A1 publication Critical patent/CA2711895A1/en
Application granted granted Critical
Publication of CA2711895C publication Critical patent/CA2711895C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C07K14/08—RNA viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203—Virus mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C12N15/8258—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon for the production of oral vaccines (antigens) or immunoglobulins

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
CA2711895A 2008-01-08 2009-01-08 Methods for protein production employing expression enhancer sequences derived from the rna-z segment of a comoviridae bipartite rna virus Active CA2711895C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0800272.7 2008-01-08
GBGB0800272.7A GB0800272D0 (en) 2008-01-08 2008-01-08 Protein expression systems
PCT/GB2009/000060 WO2009087391A1 (en) 2008-01-08 2009-01-08 Protein expression systems

Publications (2)

Publication Number Publication Date
CA2711895A1 CA2711895A1 (en) 2009-07-16
CA2711895C true CA2711895C (en) 2016-08-09

Family

ID=39111256

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2711895A Active CA2711895C (en) 2008-01-08 2009-01-08 Methods for protein production employing expression enhancer sequences derived from the rna-z segment of a comoviridae bipartite rna virus

Country Status (18)

Country Link
US (5) US8674084B2 (https=)
EP (1) EP2240589B1 (https=)
JP (1) JP5745857B2 (https=)
KR (1) KR101602972B1 (https=)
CN (1) CN101952446B (https=)
AU (1) AU2009203608B2 (https=)
BR (1) BRPI0906509A2 (https=)
CA (1) CA2711895C (https=)
DK (1) DK2240589T3 (https=)
ES (1) ES2537198T3 (https=)
GB (1) GB0800272D0 (https=)
IL (1) IL206606A (https=)
MX (1) MX2010007336A (https=)
NZ (1) NZ586939A (https=)
PT (1) PT2240589E (https=)
RU (1) RU2539793C2 (https=)
WO (1) WO2009087391A1 (https=)
ZA (1) ZA201005245B (https=)

Families Citing this family (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2615372A1 (en) 2007-07-13 2009-01-13 Marc-Andre D'aoust Influenza virus-like particles (vlps) comprising hemagglutinin
WO2009076778A1 (en) 2007-11-27 2009-06-25 Medicago Inc. Recombinant influenza virus-like particles (vlps) produced in transgenic plants expressing hemagglutinin
GB0800272D0 (en) 2008-01-08 2008-02-13 Plant Bioscience Ltd Protein expression systems
AU2009267769B2 (en) 2008-07-08 2015-07-16 Medicago Inc. Soluble recombinant influenza antigens
HUE039100T2 (hu) 2009-06-24 2018-12-28 Medicago Inc Hemagglutinint tartalmazó kimer influenzavírus-jellegû részecskék
GB201002434D0 (en) * 2010-02-12 2010-03-31 Plant Bioscience Ltd Non-infectious RNA source
WO2012058762A1 (en) 2010-11-04 2012-05-10 Medicago Inc. Plant expression system
PT2665818T (pt) 2011-01-17 2017-07-05 Philip Morris Products Sa Expressão de proteínas em plantas
CA2824155A1 (en) 2011-01-17 2012-07-26 Philip Morris Products S.A. Vectors for nucleic acid expression in plants
US11390878B2 (en) 2011-09-30 2022-07-19 Medicago Inc. Increasing protein yield in plants
NZ622731A (en) 2011-09-30 2016-04-29 Medicago Inc Increasing virus-like particle yield in plants
BR112014011345A2 (pt) * 2011-11-11 2017-05-16 Philip Morris Products Sa partículas tipo vírus da influenza (vlps) compreendendo nicotiana tabacum produzida por hemaglutinina
JP2015528285A (ja) * 2012-09-05 2015-09-28 メディカゴ インコーポレイテッド 植物におけるピコルナウイルス様粒子の産生
KR101449155B1 (ko) * 2012-12-06 2014-10-13 주식회사 바이오앱 식물체에서 목적 단백질의 번역 효율을 증진시키기 위한 염기서열
US12467058B2 (en) 2013-03-28 2025-11-11 Aramis Biotechnologies Inc. Influenza virus-like particle production in plants
ES2803508T3 (es) 2013-03-28 2021-01-27 Medicago Inc Producción de partículas similares al virus de la gripe en plantas
RU2699982C2 (ru) * 2014-01-10 2019-09-11 Медикаго Инк. Энхансерные элементы cpmv
US10563213B2 (en) 2014-03-27 2020-02-18 Medicago Inc. Modified CPMV enhancer elements
RU2731295C2 (ru) 2014-07-11 2020-09-01 Медикаго Инк. Модификация продуцирования белков в растениях
AU2016208999A1 (en) 2015-01-23 2017-07-13 Medicago Inc. Rotavirus-like particle production in plants
JP2018524345A (ja) 2015-07-02 2018-08-30 メディカゴ インコーポレイテッド ジャスモン酸経路アクチベーター
CN110506118A (zh) * 2017-02-02 2019-11-26 杜克大学 用于控制基因表达的组合物和方法
GB201721600D0 (en) 2017-12-21 2018-02-07 Plant Bioscience Ltd Metabolic engineering
CN112400021A (zh) 2018-03-14 2021-02-23 麦迪卡格公司 植物表达增强子
GB201808617D0 (en) 2018-05-25 2018-07-11 Plant Bioscience Ltd Scaffold modification
WO2020033416A1 (en) * 2018-08-06 2020-02-13 Northwestern University Combined transcription and translation platform derived from plant chloroplasts
WO2020181354A1 (en) 2019-03-14 2020-09-17 Mitsubishi Tanabe Pharma Corporation Endogenous plant expression enhancer
GB201908431D0 (en) 2019-06-12 2019-07-24 Plant Bioscience Ltd Biosynthetic genes and polypeptides
CN114040979B (zh) * 2019-06-21 2024-06-28 国立大学法人大阪大学 稳定地保持外源基因的人工重组rna病毒的制作方法
GB201909104D0 (en) 2019-06-25 2019-08-07 Plant Bioscience Ltd Transferase enzymes
CN110468129B (zh) * 2019-08-08 2023-03-24 四川农业大学 一种基于cmv rna2的体外干扰黄瓜花叶病的制剂、其制备方法与应用
CN111777670B (zh) * 2020-06-19 2021-07-30 东北农业大学 一种pH调节自组装抗菌肽及制备方法和应用
WO2022260849A1 (en) 2021-06-09 2022-12-15 Nant Holdings Ip, Llc Methods and systems for producing a protein of interest in a plant
CN114181969B (zh) * 2021-12-09 2024-05-28 苏州大学 一种制备包裹外源蛋白的多角体的方法
GB202209501D0 (en) 2022-06-29 2022-08-10 Plant Bioscience Ltd Biosynthetic enzymes
CN121195070A (zh) 2023-04-03 2025-12-23 斯威夫特制药有限责任公司 用于在植物中高效异源基因表达的反式互补载体系统
KR20250082120A (ko) * 2023-11-29 2025-06-09 서강대학교산학협력단 검은눈콩 모자이크 바이러스의 바이러스 유사 입자의 제조방법 및 이의 활용

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19701141C1 (de) * 1997-01-16 1998-04-09 Hoechst Ag Genkonstrukte für durch Proteasen aktivierbare Wirksubstanzen
AU7373801A (en) * 2000-06-13 2001-12-24 Univ Queensland Expression modulating sequences
CA2651907C (en) * 2006-05-22 2016-12-20 Plant Bioscience Limited Bipartite system, method and composition for the constitutive and inducible expression of high levels of foreign proteins in plants
GB0800272D0 (en) 2008-01-08 2008-02-13 Plant Bioscience Ltd Protein expression systems
RU2699982C2 (ru) 2014-01-10 2019-09-11 Медикаго Инк. Энхансерные элементы cpmv
US10563213B2 (en) 2014-03-27 2020-02-18 Medicago Inc. Modified CPMV enhancer elements

Also Published As

Publication number Publication date
US20140250549A1 (en) 2014-09-04
US20200392522A1 (en) 2020-12-17
KR101602972B1 (ko) 2016-03-11
EP2240589B1 (en) 2015-03-04
MX2010007336A (es) 2010-08-16
EP2240589A1 (en) 2010-10-20
RU2010133217A (ru) 2012-02-20
IL206606A (en) 2015-11-30
ES2537198T3 (es) 2015-06-03
BRPI0906509A2 (pt) 2015-12-01
CN101952446B (zh) 2014-06-11
AU2009203608A1 (en) 2009-07-16
CN101952446A (zh) 2011-01-19
US8674084B2 (en) 2014-03-18
JP5745857B2 (ja) 2015-07-08
JP2011509081A (ja) 2011-03-24
DK2240589T3 (en) 2015-05-04
US10822613B2 (en) 2020-11-03
IL206606A0 (en) 2010-12-30
AU2009203608B2 (en) 2014-03-27
KR20100109950A (ko) 2010-10-11
CA2711895A1 (en) 2009-07-16
US20210155947A1 (en) 2021-05-27
US10947549B2 (en) 2021-03-16
PT2240589E (pt) 2015-06-18
US20180030462A1 (en) 2018-02-01
NZ586939A (en) 2012-08-31
US20100287670A1 (en) 2010-11-11
ZA201005245B (en) 2012-01-25
RU2539793C2 (ru) 2015-01-27
WO2009087391A1 (en) 2009-07-16
GB0800272D0 (en) 2008-02-13

Similar Documents

Publication Publication Date Title
US10947549B2 (en) Protein expression systems
Gleba et al. Engineering viral expression vectors for plants: the ‘full virus’ and the ‘deconstructed virus’ strategies
US8936937B2 (en) System for expression of genes in plants from a virus-based expression vector
JP2004531260A (ja) 植物における対象とする核酸配列の増幅及び発現に用いる方法及びベクター
EP2029755A1 (en) Bipartite system, method and composition for the constitutive and inducible expression of high levels of foreign proteins in plants
MXPA01007256A (es) Metodos para expresion transgenica condicional y caracteristicas movibles en plantas.
US20070300330A1 (en) Two-Component Rna Virus-Derived Plant Expression System
CN109880846B (zh) 一种用于植物基因组编辑载体、及其构建方法和应用
CN106460005B (zh) 修饰的cpmv增强子元件
RU2326167C2 (ru) Искусственный промотор для экспрессии последовательностей днк в растительных клетках
WO2024208914A1 (en) Trans-complementary vector system for highly efficient heterologous gene expression in plants
CN110423775B (zh) 一种水稻基因组中稻瘟病抗性位点dna的编辑修饰方法、编辑载体
AU4310199A (en) Improved methods and materials for transformation

Legal Events

Date Code Title Description
EEER Examination request
MPN Maintenance fee for patent paid

Free format text: FEE DESCRIPTION TEXT: MF (PATENT, 16TH ANNIV.) - STANDARD

Year of fee payment: 16

U00 Fee paid

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED

Effective date: 20241217

U11 Full renewal or maintenance fee paid

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT DETERMINED COMPLIANT

Effective date: 20241217