AU2006291780B2 - Cleavage of precursors of insulins by a variant of trypsin - Google Patents
Cleavage of precursors of insulins by a variant of trypsin Download PDFInfo
- Publication number
- AU2006291780B2 AU2006291780B2 AU2006291780A AU2006291780A AU2006291780B2 AU 2006291780 B2 AU2006291780 B2 AU 2006291780B2 AU 2006291780 A AU2006291780 A AU 2006291780A AU 2006291780 A AU2006291780 A AU 2006291780A AU 2006291780 B2 AU2006291780 B2 AU 2006291780B2
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- Prior art keywords
- insulin
- trypsin
- human insulin
- cleavage
- variant
- Prior art date
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- 108090001061 Insulin Proteins 0.000 title claims abstract description 39
- 239000002243 precursor Substances 0.000 title claims abstract description 15
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-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6427—Chymotrypsins (3.4.21.1; 3.4.21.2); Trypsin (3.4.21.4)
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Diabetes (AREA)
- Endocrinology (AREA)
- Toxicology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP05077086 | 2005-09-14 | ||
| EP05077086.6 | 2005-09-14 | ||
| PCT/EP2006/008380 WO2007031187A1 (fr) | 2005-09-14 | 2006-08-26 | Clivage de precurseurs d'insulines par un variant de la trypsine |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU2006291780A1 AU2006291780A1 (en) | 2007-03-22 |
| AU2006291780B2 true AU2006291780B2 (en) | 2011-12-08 |
Family
ID=36190640
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2006291780A Active AU2006291780B2 (en) | 2005-09-14 | 2006-08-26 | Cleavage of precursors of insulins by a variant of trypsin |
Country Status (20)
| Country | Link |
|---|---|
| US (1) | US7981635B2 (fr) |
| EP (1) | EP1926749B1 (fr) |
| JP (1) | JP5027812B2 (fr) |
| KR (1) | KR101304958B1 (fr) |
| CN (1) | CN101253196B (fr) |
| AR (1) | AR055428A1 (fr) |
| AT (1) | ATE517919T1 (fr) |
| AU (1) | AU2006291780B2 (fr) |
| BR (1) | BRPI0616054B8 (fr) |
| CA (1) | CA2621344C (fr) |
| CY (1) | CY1112667T1 (fr) |
| ES (1) | ES2370657T3 (fr) |
| HK (1) | HK1124341A1 (fr) |
| IL (1) | IL189887A (fr) |
| MX (1) | MX2008003362A (fr) |
| MY (1) | MY146082A (fr) |
| PL (1) | PL1926749T3 (fr) |
| PT (1) | PT1926749E (fr) |
| SI (1) | SI1926749T1 (fr) |
| WO (1) | WO2007031187A1 (fr) |
Families Citing this family (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102007143B (zh) | 2008-01-09 | 2015-08-26 | 塞诺菲-安万特德国有限公司 | 具有超延迟时效特征的新型胰岛素衍生物 |
| AU2009203810B2 (en) | 2008-01-09 | 2013-07-25 | Sanofi-Aventis Deutschland Gmbh | Novel insulin derivatives having an extremely delayed time-action profile |
| DE102008025007A1 (de) | 2008-05-24 | 2009-11-26 | Sanofi-Aventis Deutschland Gmbh | Neue Insulinderivate mit extrem verzögertem Zeit-/ Wirkungsprofil |
| DE102008025008A1 (de) | 2008-05-24 | 2009-11-26 | Sanofi-Aventis Deutschland Gmbh | Neue Insulinderivate mit extrem verzögertem Zeit-/ Wirkungsprofil |
| DE102008003566A1 (de) | 2008-01-09 | 2009-07-16 | Sanofi-Aventis Deutschland Gmbh | Neue Insulinderivate mit extrem verzögertem Zeit-/ Wirkungsprofil |
| DE102008003568A1 (de) | 2008-01-09 | 2009-07-16 | Sanofi-Aventis Deutschland Gmbh | Neue Insulinderivate mit extrem verzögertem Zeit-/ Wirkungsprofil |
| US8802816B2 (en) * | 2008-02-19 | 2014-08-12 | Biocon Limited | Method of obtaining a purified, biologically active heterologous protein |
| PT3228320T (pt) | 2008-10-17 | 2020-03-26 | Sanofi Aventis Deutschland | Combinação de uma insulina e de um agonista do glp-1 |
| WO2011030347A1 (fr) * | 2009-09-10 | 2011-03-17 | Biocon Limited | Nouvelles protéines de fusion prolipase-trypsinogène bovin |
| RU2537239C2 (ru) | 2009-11-13 | 2014-12-27 | Санофи-Авентис Дойчланд Гмбх | Фармацевтическая композиция, включающая агонист glp-1, инсулин и метионин |
| PT2498801T (pt) | 2009-11-13 | 2018-05-02 | Sanofi Aventis Deutschland | Composição farmacêutica compreendendo despro36exendin-4(1-39)-lys6-nh2 e metionina |
| RU2546520C2 (ru) | 2010-08-30 | 2015-04-10 | Санофи-Авентис Дойчланд Гмбх | Применение ave0010 для производства лекарственного средства для лечения сахарного диабета 2 типа |
| US9821032B2 (en) | 2011-05-13 | 2017-11-21 | Sanofi-Aventis Deutschland Gmbh | Pharmaceutical combination for improving glycemic control as add-on therapy to basal insulin |
| WO2013015697A1 (fr) * | 2011-07-28 | 2013-01-31 | Mabion S.A. | Protéine recombinante, polynucléotide codant pour celle-ci ainsi que procédé d'obtention d'insuline ou de son analogue |
| ES2550357T3 (es) | 2011-08-29 | 2015-11-06 | Sanofi-Aventis Deutschland Gmbh | Combinación farmacéutica para su uso en el control glucémico en pacientes de diabetes de tipo 2 |
| TWI559929B (en) | 2011-09-01 | 2016-12-01 | Sanofi Aventis Deutschland | Pharmaceutical composition for use in the treatment of a neurodegenerative disease |
| US9388227B2 (en) * | 2012-03-29 | 2016-07-12 | Biocon Limited | Process of expressing a fully folded functional two chain insulin glargine |
| CN102816785B (zh) * | 2012-05-24 | 2016-03-09 | 上海仁会生物制药股份有限公司 | 一种甘精胰岛素及其类似物的制备方法 |
| CN102994600A (zh) * | 2012-12-11 | 2013-03-27 | 鲁南新时代生物技术有限公司 | 甘精胰岛素前体的酶切转化方法 |
| HUE062573T2 (hu) | 2014-12-12 | 2023-11-28 | Sanofi Aventis Deutschland | Glargin inzulin/lixiszenatid rögzített arányú készítmény |
| TWI748945B (zh) | 2015-03-13 | 2021-12-11 | 德商賽諾菲阿凡提斯德意志有限公司 | 第２型糖尿病病患治療 |
| TW201705975A (zh) | 2015-03-18 | 2017-02-16 | 賽諾菲阿凡提斯德意志有限公司 | 第２型糖尿病病患之治療 |
| CN108018277A (zh) * | 2016-11-04 | 2018-05-11 | 江苏万邦生化医药股份有限公司 | 重组牛胰蛋白酶突变体及其制备方法 |
| KR20190036956A (ko) | 2017-09-28 | 2019-04-05 | 한미약품 주식회사 | 지속형 단쇄 인슐린 아날로그 및 이의 결합체 |
| US11518988B2 (en) * | 2018-02-22 | 2022-12-06 | Sanofi-Aventis Deutschland Gmbh | Variants of porcine trypsin |
| EP3650541A1 (fr) * | 2018-11-08 | 2020-05-13 | BioPharma Translationsinstitut Dessau Forschungs GmbH | Mutant de trypsine pour la modification de polypeptides par les terminaux c et n |
| KR20200080747A (ko) * | 2018-12-27 | 2020-07-07 | 주식회사 폴루스 | 인슐린 전구체의 인슐린 효소 전환용 조성물 및 이를 이용하여 인슐린 전구체를 인슐린으로 전환하는 방법 |
| IL293635A (en) | 2019-12-10 | 2022-08-01 | Sanofi Sa | A method for preparing a conjugate of a sulfonamide and a polypeptide |
| CN113773391B (zh) * | 2020-06-09 | 2023-10-20 | 宁波鲲鹏生物科技有限公司 | 一种门冬胰岛素的制备方法 |
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| US3886043A (en) * | 1972-08-24 | 1975-05-27 | Choay Sa | Process for preparing pure porcine beta-trypsin |
| NZ199391A (en) | 1981-01-02 | 1985-12-13 | Genentech Inc | Chimeric polypeptides comprising a proinsulin sequence,and preparation by recombinant dna technique;production of human insulin |
| NZ199722A (en) | 1981-02-25 | 1985-12-13 | Genentech Inc | Dna transfer vector for expression of exogenous polypeptide in yeast;transformed yeast strain |
| DE3382547D1 (de) | 1983-01-12 | 1992-05-27 | Chiron Corp | Sekretorische expression in eukaryoten. |
| US4870008A (en) | 1983-08-12 | 1989-09-26 | Chiron Corporation | Secretory expression in eukaryotes |
| US4885242A (en) | 1984-10-30 | 1989-12-05 | Phillips Petroleum Company | Genes from pichia histidine pathway and uses thereof |
| US4879231A (en) | 1984-10-30 | 1989-11-07 | Phillips Petroleum Company | Transformation of yeasts of the genus pichia |
| US4855231A (en) | 1984-10-30 | 1989-08-08 | Phillips Petroleum Company | Regulatory region for heterologous gene expression in yeast |
| US4837148A (en) | 1984-10-30 | 1989-06-06 | Phillips Petroleum Company | Autonomous replication sequences for yeast strains of the genus pichia |
| US4808537A (en) | 1984-10-30 | 1989-02-28 | Phillips Petroleum Company | Methanol inducible genes obtained from pichia and methods of use |
| PH25772A (en) | 1985-08-30 | 1991-10-18 | Novo Industri As | Insulin analogues, process for their preparation |
| US5166329A (en) | 1985-10-25 | 1992-11-24 | Phillips Petroleum Company | DNA encoding the alcohol oxidase 2 gene of yeast of the genus Pichia |
| US5032516A (en) | 1985-10-25 | 1991-07-16 | Phillips Petroleum Company | Pichia pastoris alcohol oxidase II regulatory region |
| US4882279A (en) | 1985-10-25 | 1989-11-21 | Phillips Petroleum Company | Site selective genomic modification of yeast of the genus pichia |
| US5135868A (en) | 1985-10-25 | 1992-08-04 | Phillips Petroleum Company | Cultures of yeast of the genus Pichia altered by site selective genomic modification |
| US4818700A (en) | 1985-10-25 | 1989-04-04 | Phillips Petroleum Company | Pichia pastoris argininosuccinate lyase gene and uses thereof |
| US4895800A (en) | 1985-11-26 | 1990-01-23 | Phillips Petroleum Company | Yeast production of hepatitis B surface antigen |
| US4812405A (en) | 1986-02-18 | 1989-03-14 | Phillips Petroleum Company | Double auxotrophic mutants of Pichia pastoris and methods for preparation |
| US4857467A (en) | 1986-07-23 | 1989-08-15 | Phillips Petroleum Company | Carbon and energy source markers for transformation of strains of the genes Pichia |
| US5002876A (en) | 1986-09-22 | 1991-03-26 | Phillips Petroleum Company | Yeast production of human tumor necrosis factor |
| US4683293A (en) | 1986-10-20 | 1987-07-28 | Phillips Petroleum Company | Purification of pichia produced lipophilic proteins |
| US4929555A (en) | 1987-10-19 | 1990-05-29 | Phillips Petroleum Company | Pichia transformation |
| US5004688A (en) | 1988-04-15 | 1991-04-02 | Phillips Petroleum Company | Purification of hepatitis proteins |
| DK257988D0 (da) | 1988-05-11 | 1988-05-11 | Novo Industri As | Nye peptider |
| JPH04502465A (ja) | 1988-12-23 | 1992-05-07 | ノボ ノルディスク アクティーゼルスカブ | ヒトインシュリン類似物質 |
| IL93282A (en) | 1989-02-09 | 1995-08-31 | Lilly Co Eli | Insulin analogues |
| US5122465A (en) | 1989-06-12 | 1992-06-16 | Phillips Petroleum Company | Strains of pichia pastoris created by interlocus recombination |
| WO1992004363A1 (fr) | 1990-09-04 | 1992-03-19 | The Salk Institute Biotechnology/Industrial Associates, Inc. | Production du facteur-1 de croissance similaire a l'insuline dans des cellules de levure methylotrophique |
| DE19821866A1 (de) * | 1998-05-15 | 1999-11-18 | Hoechst Marion Roussel De Gmbh | Verfahren zur Katalyse von komplexen Reaktionen großer Moleküle mittels an einem polymeren Träger gebundener Enzyme |
| AU3910000A (en) | 1999-03-22 | 2000-10-09 | Zymogenetics Inc. | Improved methods for producing proteins in transformed (pichia) |
| DK1399568T3 (da) * | 2001-02-01 | 2006-03-27 | Hoffmann La Roche | Fremgangsmåde til fremstilling af rekombinant trypsin |
| ATE452184T1 (de) | 2002-09-24 | 2010-01-15 | Novozymes Inc | Mikrobielle trypsinvarianten mit chymotrypsinaktivität und diese codierende nukleinsäuren |
-
2006
- 2006-08-26 MX MX2008003362A patent/MX2008003362A/es active IP Right Grant
- 2006-08-26 BR BRPI0616054A patent/BRPI0616054B8/pt active IP Right Grant
- 2006-08-26 KR KR1020087006393A patent/KR101304958B1/ko active IP Right Grant
- 2006-08-26 MY MYPI20080235A patent/MY146082A/en unknown
- 2006-08-26 SI SI200631146T patent/SI1926749T1/sl unknown
- 2006-08-26 CA CA2621344A patent/CA2621344C/fr active Active
- 2006-08-26 PL PL06791678T patent/PL1926749T3/pl unknown
- 2006-08-26 AU AU2006291780A patent/AU2006291780B2/en active Active
- 2006-08-26 WO PCT/EP2006/008380 patent/WO2007031187A1/fr active Application Filing
- 2006-08-26 JP JP2008530363A patent/JP5027812B2/ja active Active
- 2006-08-26 PT PT06791678T patent/PT1926749E/pt unknown
- 2006-08-26 ES ES06791678T patent/ES2370657T3/es active Active
- 2006-08-26 CN CN200680031396XA patent/CN101253196B/zh active Active
- 2006-08-26 EP EP06791678A patent/EP1926749B1/fr active Active
- 2006-08-26 US US12/063,313 patent/US7981635B2/en active Active
- 2006-08-26 AT AT06791678T patent/ATE517919T1/de active
- 2006-09-12 AR ARP060103963A patent/AR055428A1/es active IP Right Grant
-
2008
- 2008-03-02 IL IL189887A patent/IL189887A/en active IP Right Grant
-
2009
- 2009-02-11 HK HK09101249.8A patent/HK1124341A1/xx unknown
-
2011
- 2011-10-27 CY CY20111101023T patent/CY1112667T1/el unknown
Non-Patent Citations (1)
| Title |
|---|
| Sichler K. et al. FEBS Letters (2002) 530 (1-3): 220-224 * |
Also Published As
| Publication number | Publication date |
|---|---|
| MY146082A (en) | 2012-06-29 |
| JP5027812B2 (ja) | 2012-09-19 |
| BRPI0616054B8 (pt) | 2021-05-25 |
| CA2621344A1 (fr) | 2007-03-22 |
| AR055428A1 (es) | 2007-08-22 |
| US20100196953A1 (en) | 2010-08-05 |
| PT1926749E (pt) | 2011-09-29 |
| US7981635B2 (en) | 2011-07-19 |
| CA2621344C (fr) | 2014-12-16 |
| EP1926749A1 (fr) | 2008-06-04 |
| PL1926749T3 (pl) | 2011-12-30 |
| AU2006291780A1 (en) | 2007-03-22 |
| EP1926749B1 (fr) | 2011-07-27 |
| ES2370657T3 (es) | 2011-12-21 |
| CY1112667T1 (el) | 2016-02-10 |
| BRPI0616054B1 (pt) | 2019-08-06 |
| JP2009507500A (ja) | 2009-02-26 |
| ATE517919T1 (de) | 2011-08-15 |
| SI1926749T1 (sl) | 2011-11-30 |
| CN101253196B (zh) | 2012-09-05 |
| WO2007031187A1 (fr) | 2007-03-22 |
| BRPI0616054A2 (pt) | 2011-06-07 |
| HK1124341A1 (en) | 2009-07-10 |
| IL189887A (en) | 2012-02-29 |
| WO2007031187A8 (fr) | 2007-08-16 |
| MX2008003362A (es) | 2008-03-25 |
| CN101253196A (zh) | 2008-08-27 |
| KR20080055843A (ko) | 2008-06-19 |
| IL189887A0 (en) | 2008-11-03 |
| KR101304958B1 (ko) | 2013-09-17 |
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