AU2004263813B8 - Methods of producing influenza vaccine compositions - Google Patents
Methods of producing influenza vaccine compositions Download PDFInfo
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- AU2004263813B8 AU2004263813B8 AU2004263813A AU2004263813A AU2004263813B8 AU 2004263813 B8 AU2004263813 B8 AU 2004263813B8 AU 2004263813 A AU2004263813 A AU 2004263813A AU 2004263813 A AU2004263813 A AU 2004263813A AU 2004263813 B8 AU2004263813 B8 AU 2004263813B8
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- influenza virus
- influenza
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Families Citing this family (86)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK1499348T3 (en) | 2002-04-26 | 2015-01-05 | Medimmune Llc | PROCEDURE FOR PREPARING INFECTIOUS INFLUENZA B-VIRA IN CELL CULTURE |
| US7465456B2 (en) | 2002-04-26 | 2008-12-16 | Medimmune, Llc | Multi plasmid system for the production of influenza virus |
| CA2491145A1 (en) * | 2002-07-03 | 2004-01-15 | Basf Aktiengesellschaft | Microorganisms and processes for enhanced production of pantothenate |
| US20060110406A1 (en) | 2003-02-25 | 2006-05-25 | Medimmune Vaccines, Inc. | Refrigerator-temperature stable influenza vaccine compositions |
| EP2481819A1 (en) | 2003-02-25 | 2012-08-01 | MedImmune Vaccines, Inc. | Cross flow filtration in the production of stabilized influenza vaccine compositions |
| US7566458B2 (en) | 2003-06-16 | 2009-07-28 | Medimmune, Llc | Influenza hemagglutinin and neuraminidase variants |
| CA2551489C (en) | 2003-12-23 | 2013-09-03 | Gregory Duke | Multi plasmid system for the production of influenza virus |
| SG150501A1 (en) * | 2004-02-05 | 2009-03-30 | Millipore Corp | Porous adsorptive or chromatographic media |
| EP2769735B1 (en) * | 2004-10-06 | 2017-04-19 | MedImmune, LLC | Refrigerator-temperature stable influenza vaccine compositions |
| US7510719B2 (en) | 2004-12-08 | 2009-03-31 | Medimmune, Llc | Methods of producing influenza vaccine compositions |
| DK2368975T3 (en) | 2004-12-23 | 2015-01-05 | Medimmune Llc | Non-tumorigenic MDCK cell line for the propagation of viruses |
| EP2471552B1 (en) | 2005-03-08 | 2015-05-06 | MedImmune, LLC | Reassortant influenza viruses |
| DK1869471T3 (en) * | 2005-04-13 | 2016-08-01 | Merial Inc | PROCEDURE FOR MANUFACTURING PIG CIRCOVIRUS |
| US20060275170A1 (en) * | 2005-06-02 | 2006-12-07 | Mahmoud Ameri | Method for terminal sterilization of transdermal delivery devices |
| US7790434B2 (en) * | 2005-06-21 | 2010-09-07 | Medimmune, Llc | Methods and compositions for expressing negative-sense viral RNA in canine cells |
| EP1917352A4 (en) * | 2005-06-21 | 2009-03-25 | Medimmune Vaccines Inc | METHODS AND COMPOSITIONS FOR EXPRESSING HETEROLOGOUS PROTEASE |
| WO2007019232A2 (en) * | 2005-08-03 | 2007-02-15 | Immunogen, Inc. | Immunoconjugate formulations |
| GB0613977D0 (en) | 2006-02-07 | 2006-08-23 | Peptcell Ltd | Peptide sequences and compositions |
| US7534596B2 (en) * | 2006-02-10 | 2009-05-19 | Solohill Engineering, Inc. | Method and device for producing vaccine |
| DK2016194T3 (en) | 2006-04-19 | 2015-06-15 | Medimmune Llc | METHODS AND COMPOSITIONS FOR EXPRESSION OF VIRAL NEGATIVE SENSE RNA IN DOG CELLS |
| GB0614460D0 (en) * | 2006-07-20 | 2006-08-30 | Novartis Ag | Vaccines |
| EP2615167A1 (en) | 2006-09-15 | 2013-07-17 | MedImmune, LLC | Method for eliminating DNA contaminants from viral preparations |
| JP2010512748A (ja) * | 2006-12-15 | 2010-04-30 | シェーリング−プラウ・リミテッド | インフルエンザウイルスを培養物中で複製するための方法 |
| AU2008225501B2 (en) * | 2007-03-09 | 2013-08-29 | Otsuka Pharmaceutical Co., Ltd. | Lyophilized preparation comprising influenza vaccine, and method for preparation thereof |
| EP2674486A1 (en) * | 2007-06-18 | 2013-12-18 | MedImmune, LLC | Influenza B viruses having alterations in the hemaglutinin polypeptide |
| US9433922B2 (en) * | 2007-08-14 | 2016-09-06 | Emd Millipore Corporation | Media for membrane ion exchange chromatography based on polymeric primary amines, sorption device containing that media, and chromatography scheme and purification method using the same |
| CA2705461A1 (en) | 2007-11-12 | 2009-06-11 | The Trustees Of The University Of Pennsylvania | Vaccines against multiple subtypes of influenza virus |
| US20090130738A1 (en) * | 2007-11-19 | 2009-05-21 | Mikhail Kozlov | Media for membrane ion exchange chromatography |
| EP2072058A1 (en) * | 2007-12-21 | 2009-06-24 | Avir Green Hills Biotechnology Research Development Trade Ag | Modified influenza virus |
| JP2011184299A (ja) * | 2008-07-02 | 2011-09-22 | Asahi Kasei Medical Co Ltd | モノクローナル抗体を含む製剤の製造方法 |
| US8785173B2 (en) | 2008-09-24 | 2014-07-22 | Medimmune, Llc | Methods for purification of viruses |
| EP2344634A4 (en) | 2008-09-24 | 2012-08-29 | Medimmune Llc | METHODS OF CELL CULTURE, AND PROPAGATION AND PURIFICATION OF VIRUSES |
| CN102223895B (zh) * | 2008-11-25 | 2014-11-05 | 巴克斯特保健股份有限公司 | 生产pH稳定的包膜病毒的方法 |
| CN107365751B (zh) | 2008-12-16 | 2021-07-09 | 纳米医疗公司 | 流感疫苗的生产 |
| CN101450209B (zh) * | 2008-12-31 | 2012-01-25 | 中国人民解放军军事医学科学院微生物流行病研究所 | 透皮免疫流感多价疫苗的制备及其方法 |
| RU2424762C2 (ru) * | 2009-05-06 | 2011-07-27 | Валерий Денисович Белокрылов | Способ и автоматизированное устройство (два варианта) для диагностики заболеваний, вызванных вирусом a/h1n1 и его мутациями |
| WO2010144797A2 (en) | 2009-06-12 | 2010-12-16 | Vaccine Technologies, Incorporated | Influenza vaccines with enhanced immunogenicity and uses thereof |
| JP5854559B2 (ja) | 2009-07-06 | 2016-02-09 | ヴァリエーション バイオテクノロジーズ インコーポレイテッド | 小胞を調製する方法及びこれから製造される製剤 |
| CA2803282C (en) | 2009-07-06 | 2018-05-01 | David E. Anderson | Methods for preparing vesicles and formulations produced therefrom |
| US20120077249A1 (en) * | 2010-04-20 | 2012-03-29 | Millipore Corporation | Separation Of Virus And/Or Protein From Nucleic Acids By Primary Amines |
| WO2012006367A2 (en) | 2010-07-06 | 2012-01-12 | Variation Biotechnologies, Inc. | Compositions and methods for treating influenza |
| CN103282873B (zh) * | 2010-11-12 | 2016-08-24 | 生命科技公司 | 用于实验室测定确认或验证的系统和方法 |
| BR112013018074A2 (pt) | 2011-01-13 | 2020-12-01 | Variation Biotechnologies, Inc. | métodos para a preparação de vesículas e formulações produzidas a partir dessas |
| US10736844B2 (en) | 2011-01-13 | 2020-08-11 | Variation Biotechnologies Inc. | Compositions and methods for treating viral infections |
| WO2012136852A1 (en) | 2011-04-08 | 2012-10-11 | Inserm (Institut National De La Sante Et De La Recherche Medicale) | Method for increasing the replication capacity of an influenza virus in cultured cells |
| EP2710381A2 (en) | 2011-05-16 | 2014-03-26 | Institut National de la Sante et de la Recherche Medicale (INSERM) | Methods for screening substances capable of modulating the replication of an influenza virus |
| CN103827296B (zh) * | 2011-07-20 | 2016-11-16 | 雅培生物学有限责任公司 | 用于产生病毒抗原和疫苗的方法 |
| US20140328876A1 (en) | 2011-11-18 | 2014-11-06 | Variation Biotechnologies Inc. | Synthetic derivatives of mpl and uses thereof |
| CN104023744A (zh) * | 2011-12-23 | 2014-09-03 | 诺华股份有限公司 | 用于针对金黄色葡萄球菌免疫的稳定组合物 |
| AU2013208693B2 (en) | 2012-01-12 | 2017-12-07 | Variation Biotechnologies Inc. | Compositions and methods for treating viral infections |
| EP2806894A4 (en) | 2012-01-27 | 2015-11-04 | Variation Biotechnologies Inc | METHODS AND COMPOSITIONS FOR THERAPEUTIC AGENTS |
| US9932620B2 (en) | 2012-05-21 | 2018-04-03 | Celsis Ltd. | Methods, devices, and systems of detecting microorganisms |
| US20130309700A1 (en) * | 2012-05-21 | 2013-11-21 | Celsis International Limited | Methods, devices, and systems of detecting microorganisms |
| US9314519B2 (en) * | 2012-08-21 | 2016-04-19 | Intervet Inc. | Liquid stable virus vaccines |
| WO2014142433A1 (ko) | 2013-03-13 | 2014-09-18 | 고려대학교산학협력단 | 바이러스 생산능이 증가된 세포주 및 그 제조방법 |
| KR20140112255A (ko) | 2013-03-13 | 2014-09-23 | 고려대학교 산학협력단 | 바이러스 생산능이 증가된 세포주 및 그 제조방법 |
| US9480739B2 (en) | 2013-03-15 | 2016-11-01 | Intervet Inc. | Bovine virus vaccines that are liquid stable |
| US9393298B2 (en) | 2013-03-15 | 2016-07-19 | Intervet Inc. | Liquid stable bovine virus vaccines |
| WO2015017673A1 (en) * | 2013-08-01 | 2015-02-05 | Medimmune, Llc | Methods for producing influenza vaccine compositions |
| AR097762A1 (es) | 2013-09-27 | 2016-04-13 | Intervet Int Bv | Formulaciones secas de vacunas que son estables a temperatura ambiente |
| US9579374B2 (en) | 2013-12-04 | 2017-02-28 | The Johns Hopkins University | Method for rapidly designing pharmaceutical preparations for preventing viral infection |
| AR099470A1 (es) | 2014-02-17 | 2016-07-27 | Intervet Int Bv | Vacunas de virus de aves de corral líquidas |
| TWI670085B (zh) * | 2014-02-19 | 2019-09-01 | 荷蘭商英特威國際公司 | 液體穩定之豬病毒疫苗 |
| WO2015148704A1 (en) | 2014-03-25 | 2015-10-01 | Terumo Bct, Inc. | Passive replacement of media |
| JP6550072B2 (ja) * | 2014-04-03 | 2019-07-24 | バイオンドバックス ファーマシューティカルズ リミテッド | 多量体マルチエピトープインフルエンザポリペプチドの組成物及びそれらの生成 |
| WO2015194770A1 (ko) | 2014-06-18 | 2015-12-23 | 고려대학교산학협력단 | Bst2 유전자의 기능이 상실된 세포주를 이용한 바이러스 감염 촉진 및 생산 증진 방법 |
| KR102507483B1 (ko) | 2014-12-01 | 2023-03-07 | 트랜스진 | 안정한 액체 백시니아 바이러스 제제 |
| EP4523756A3 (en) | 2015-02-13 | 2025-05-28 | Transgene | Immunotherapeutic vaccine and antibody combination therapy |
| CN104984357B (zh) * | 2015-06-02 | 2018-01-30 | 长春百克生物科技股份公司 | 不含明胶的疫苗保护剂组合物及流感减毒活疫苗 |
| WO2017004592A1 (en) | 2015-07-02 | 2017-01-05 | Terumo Bct, Inc. | Cell growth with mechanical stimuli |
| EP3381468A4 (en) * | 2015-11-27 | 2019-06-05 | Nitto Denko Corporation | DRIED INFLUENZA VACCINE PREPARATION AND METHOD FOR PRODUCING A DRIED INFLUENZED VACCINE PREPARATION |
| CA3023022A1 (en) | 2016-05-04 | 2017-11-09 | Transgene Sa | Combination therapy with cpg tlr9 ligand |
| US11965175B2 (en) | 2016-05-25 | 2024-04-23 | Terumo Bct, Inc. | Cell expansion |
| US11104874B2 (en) | 2016-06-07 | 2021-08-31 | Terumo Bct, Inc. | Coating a bioreactor |
| US11685883B2 (en) | 2016-06-07 | 2023-06-27 | Terumo Bct, Inc. | Methods and systems for coating a cell growth surface |
| WO2018069316A2 (en) | 2016-10-10 | 2018-04-19 | Transgene Sa | Immunotherapeutic product and mdsc modulator combination therapy |
| CN117247899A (zh) | 2017-03-31 | 2023-12-19 | 泰尔茂比司特公司 | 细胞扩增 |
| CA3062549A1 (en) | 2017-05-15 | 2018-11-22 | Janssen Vaccines & Prevention B.V. | Stable virus-containing composition |
| EP3624851A1 (en) | 2017-05-15 | 2020-03-25 | Janssen Vaccines & Prevention B.V. | Stable virus-containing composition |
| CA3093093A1 (en) | 2018-03-07 | 2019-09-12 | Transgene | Parapoxvirus vectors |
| US20220056481A1 (en) | 2018-12-28 | 2022-02-24 | Transgene | M2-defective poxvirus |
| WO2020136232A1 (en) | 2018-12-28 | 2020-07-02 | Transgene Sa | Immunosuppressive m2 protein |
| BR112021016059A2 (pt) * | 2019-02-15 | 2021-10-13 | Serum Institute Of India Pvt Ltd. | Composição de vacina de vírus de influenza atenuada viva e processo para a preparação da mesma |
| JP7712284B2 (ja) * | 2020-03-12 | 2025-07-23 | バヴァリアン・ノルディック・アクティーゼルスカブ | ポックスウイルスの安定性を改善する組成物 |
| WO2022148736A1 (en) | 2021-01-05 | 2022-07-14 | Transgene | Vectorization of muc1 t cell engager |
| KR20260001978A (ko) | 2024-06-28 | 2026-01-06 | 주식회사 엔에프방재 | 화재 방재용 방패 |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3874999A (en) * | 1973-10-31 | 1975-04-01 | American Cyanamid Co | Process for the purification of virus vaccine |
| US4000257A (en) * | 1975-12-22 | 1976-12-28 | American Cyanamid Company | Process for the purification of influenza virus vaccine |
Family Cites Families (40)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB660109A (en) * | 1946-10-01 | 1951-10-31 | American Cyanamid Co | Improvements in or relating to the treatment of allantoic fluid containing a propagated virus together with non-viral proteins to obtain the virus in purified and viable from for use in a vaccine |
| US4158054A (en) * | 1973-10-18 | 1979-06-12 | Duncan Flockhart & Co. Ltd. | Preparation of virus sub-unit vaccines |
| US4057626A (en) | 1976-10-08 | 1977-11-08 | Richardson-Merrell Inc. | Process for detoxifying influenza B virus |
| US4338296A (en) * | 1979-10-16 | 1982-07-06 | Smithkline-Rit | Influenza virus and process of producing a vaccine therefrom |
| JPS57114527A (en) * | 1979-10-29 | 1982-07-16 | Merck & Co Inc | Vaccine stabilizer |
| US4338335A (en) * | 1980-02-05 | 1982-07-06 | Merck & Co., Inc. | Vaccine stabilizer containing L-glutamic acid and L-arginine |
| US4337242A (en) * | 1980-02-05 | 1982-06-29 | Merck & Co., Inc. | Vaccine stabilizer containing L-glutamic acid and L-arginine |
| US4512972A (en) * | 1980-06-30 | 1985-04-23 | Kureha Chemical Industry Co., Ltd. | Nasal preparation and processes for their production |
| FR2505657A1 (fr) | 1981-05-13 | 1982-11-19 | Pasteur Institut | Perfectionnements apportes aux agents de stabilisation de virus vivants pour la preparation de vaccins, et vaccins stabilises contenant lesdits agents de stabilisation |
| US4512285A (en) * | 1983-12-27 | 1985-04-23 | Gof Manufacturing Company, Inc. | Apparatus and method for egg turning during incubation |
| GB8914968D0 (en) | 1989-06-29 | 1989-08-23 | Connaught Lab | Production of virus and purification of viral envelope proteins for vaccine use |
| DE69032102T2 (de) * | 1989-08-15 | 1998-06-25 | Massachusetts Inst Technology | Stabilisierte impfstoffzusammensetzungen |
| CA2075521C (en) * | 1992-05-05 | 1995-11-28 | Kuniaki Koyama | Stabilized live vaccine |
| US7344722B1 (en) * | 1993-06-29 | 2008-03-18 | The Regents Of The University Of Michigan | Cold-adapted influenza virus |
| US5824536A (en) | 1994-08-23 | 1998-10-20 | St. Jude Children's Research Hospital | Influenza virus replicated in mammalian cell culture and vaccine production |
| US6146873A (en) | 1994-11-10 | 2000-11-14 | Baxter Aktiengesellschaft | Production of orthomyxoviruses in monkey kidney cells using protein-free media |
| ATE213646T1 (de) | 1995-10-20 | 2002-03-15 | Yissum Res Dev Co | Grippe-impfstoff |
| DE19612966B4 (de) * | 1996-04-01 | 2009-12-10 | Novartis Vaccines And Diagnostics Gmbh & Co. Kg | MDCK-Zellen und Verfahren zur Vermehrung von Influenzaviren |
| TW570803B (en) * | 1997-04-09 | 2004-01-11 | Duphar Int Res | Influenza vaccine |
| US6177082B1 (en) * | 1998-08-13 | 2001-01-23 | The University Of Pittsburgh-Of The Commonwealth System Of Higher Education | Cold-adapted equine influenza viruses |
| NZ512980A (en) | 1998-12-17 | 2003-07-25 | Aventis Pasteur | Multivalent immunogenic composition containing RSV subunit composition and influenza virus preparation |
| GB9923176D0 (en) * | 1999-09-30 | 1999-12-01 | Smithkline Beecham Biolog | Novel composition |
| RU2002112751A (ru) * | 1999-11-12 | 2004-02-27 | Файброджен, Инк. (Us) | Рекомбинантные желатины |
| CN1147316C (zh) * | 2000-03-16 | 2004-04-28 | 辽宁天成生物制药研究所 | 流感病毒静水压技术灭活工艺制备疫苗的方法 |
| ES2319864T3 (es) | 2000-04-28 | 2009-05-14 | St. Jude Children's Research Hospital | Sistema de transfeccion de adn para la generacion de virus infecciosos de arn de cadena negativa. |
| BRPI0002694C1 (pt) | 2000-06-19 | 2021-05-25 | Fund Butantan | processo de obtenção de vacina contra raiva em células vero, para uso humano |
| JP5063852B2 (ja) | 2000-09-25 | 2012-10-31 | ポリマン サイエンティフィック イミューンバイオロジッシュ フォーシュング ゲゼルシャフト ミット ベシュレンクテル ファフツング | 生ワクチン及び製造方法 |
| EP2281573A3 (en) | 2001-02-23 | 2011-12-07 | GlaxoSmithKline Biologicals s.a. | Influenza vaccine formulations for intradermal delivery |
| MY134424A (en) | 2001-05-30 | 2007-12-31 | Saechsisches Serumwerk | Stable influenza virus preparations with low or no amount of thiomersal |
| JP2006512401A (ja) * | 2001-06-05 | 2006-04-13 | ザ リージェンツ オブ ザ ユニバーシティ オブ ミシガン | ナノエマルジョンワクチン |
| US7195905B2 (en) | 2001-12-10 | 2007-03-27 | Baxter Healthcare S.A. | Enveloped virus vaccine and method for production |
| WO2003087335A2 (en) * | 2002-04-11 | 2003-10-23 | Medimmune Vaccines, Inc. | Preservation of bioactive materials by spray drying |
| WO2003087327A2 (en) * | 2002-04-11 | 2003-10-23 | Medimmune Vaccines, Inc. | Preservation of bioactive materials by freeze dried foam |
| DK1499348T3 (en) | 2002-04-26 | 2015-01-05 | Medimmune Llc | PROCEDURE FOR PREPARING INFECTIOUS INFLUENZA B-VIRA IN CELL CULTURE |
| CN1745170A (zh) | 2002-12-17 | 2006-03-08 | 米迪缪尼疫苗股份有限公司 | 生物活性材料的高压喷雾干燥 |
| US20060110406A1 (en) * | 2003-02-25 | 2006-05-25 | Medimmune Vaccines, Inc. | Refrigerator-temperature stable influenza vaccine compositions |
| EP2481819A1 (en) | 2003-02-25 | 2012-08-01 | MedImmune Vaccines, Inc. | Cross flow filtration in the production of stabilized influenza vaccine compositions |
| CA2551489C (en) | 2003-12-23 | 2013-09-03 | Gregory Duke | Multi plasmid system for the production of influenza virus |
| HUE025727T2 (en) | 2004-05-24 | 2016-04-28 | Medimmune Llc | Multi-plasmid system for producing influenza virus |
| EP2769735B1 (en) | 2004-10-06 | 2017-04-19 | MedImmune, LLC | Refrigerator-temperature stable influenza vaccine compositions |
-
2004
- 2004-02-25 EP EP12155639A patent/EP2481819A1/en not_active Ceased
- 2004-02-25 CN CN200480011005A patent/CN100582244C/zh not_active Expired - Fee Related
- 2004-02-25 KR KR1020057015867A patent/KR101190932B1/ko not_active Expired - Fee Related
- 2004-02-25 EP EP04775805.7A patent/EP1597400B8/en not_active Expired - Lifetime
- 2004-02-25 CA CA2517181A patent/CA2517181C/en not_active Expired - Fee Related
- 2004-02-25 WO PCT/US2004/005697 patent/WO2005014862A1/en not_active Ceased
- 2004-02-25 CA CA2807534A patent/CA2807534A1/en not_active Abandoned
- 2004-02-25 CN CN200910266807.9A patent/CN101804202B/zh not_active Expired - Lifetime
- 2004-02-25 KR KR1020137013536A patent/KR101357443B1/ko not_active Expired - Lifetime
- 2004-02-25 US US10/788,236 patent/US7262045B2/en not_active Expired - Lifetime
- 2004-02-25 JP JP2006508841A patent/JP4617300B2/ja not_active Expired - Fee Related
- 2004-02-25 KR KR1020127020501A patent/KR101360300B1/ko not_active Expired - Lifetime
- 2004-02-25 AU AU2004263813A patent/AU2004263813B8/en not_active Ceased
- 2004-02-25 KR KR1020127005217A patent/KR101251902B1/ko not_active Expired - Lifetime
-
2007
- 2007-01-30 US US11/668,587 patent/US20070161085A1/en not_active Abandoned
-
2008
- 2008-10-21 AU AU2008230033A patent/AU2008230033B2/en not_active Expired
- 2008-12-08 US US12/330,299 patent/US20090246225A1/en not_active Abandoned
-
2013
- 2013-02-27 US US13/779,668 patent/US8986705B2/en not_active Expired - Lifetime
-
2015
- 2015-03-04 US US14/638,909 patent/US20160051660A1/en not_active Abandoned
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3874999A (en) * | 1973-10-31 | 1975-04-01 | American Cyanamid Co | Process for the purification of virus vaccine |
| US4000257A (en) * | 1975-12-22 | 1976-12-28 | American Cyanamid Company | Process for the purification of influenza virus vaccine |
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| DA3 | Amendments made section 104 |
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