ATE548449T1 - Pilz-peroxygenasen und anwendungsverfahren - Google Patents

Pilz-peroxygenasen und anwendungsverfahren

Info

Publication number
ATE548449T1
ATE548449T1 AT08718350T AT08718350T ATE548449T1 AT E548449 T1 ATE548449 T1 AT E548449T1 AT 08718350 T AT08718350 T AT 08718350T AT 08718350 T AT08718350 T AT 08718350T AT E548449 T1 ATE548449 T1 AT E548449T1
Authority
AT
Austria
Prior art keywords
methods
application
polypeptides
peroxygenases
mushroom
Prior art date
Application number
AT08718350T
Other languages
German (de)
English (en)
Inventor
Marek Jan Pecyna
Kirk Matthew Schnorr
Rene Ullrich
Karin Scheibner
Martin Gunter Kluge
Martin Hofrichter
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of ATE548449T1 publication Critical patent/ATE548449T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0065—Oxidoreductases (1.) acting on hydrogen peroxide as acceptor (1.11)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Detergent Compositions (AREA)
  • Pyridine Compounds (AREA)
AT08718350T 2007-03-30 2008-03-31 Pilz-peroxygenasen und anwendungsverfahren ATE548449T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102007016139A DE102007016139A1 (de) 2007-03-30 2007-03-30 Verfahren zur regioselektiven Oxygenierung von N-Heterozyklen
PCT/EP2008/053798 WO2008119780A2 (en) 2007-03-30 2008-03-31 Fungal peroxygenases and methods of application

Publications (1)

Publication Number Publication Date
ATE548449T1 true ATE548449T1 (de) 2012-03-15

Family

ID=39591196

Family Applications (1)

Application Number Title Priority Date Filing Date
AT08718350T ATE548449T1 (de) 2007-03-30 2008-03-31 Pilz-peroxygenasen und anwendungsverfahren

Country Status (9)

Country Link
US (2) US8367387B2 (enExample)
EP (3) EP2471911A3 (enExample)
JP (2) JP5411121B2 (enExample)
CN (1) CN101802180B (enExample)
AT (1) ATE548449T1 (enExample)
CA (1) CA2682451C (enExample)
DE (1) DE102007016139A1 (enExample)
DK (1) DK2158313T3 (enExample)
WO (1) WO2008119780A2 (enExample)

Families Citing this family (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2553109B1 (en) 2010-03-28 2018-10-24 Novozymes A/S Regioselective hydroxylation of aliphatic hydrocarbons employing a fungal peroxygenase
WO2013004639A2 (en) 2011-07-07 2013-01-10 Novozymes A/S Enzymatic preparation of diols
WO2013021064A1 (en) 2011-08-10 2013-02-14 Novozymes A/S Polypeptides having peroxygenase activity and polynucleotides encoding same
US9382559B2 (en) 2011-08-10 2016-07-05 Novozymes A/S Polypeptides having peroxygenase activity and polynucleotides encoding same
WO2013021060A1 (en) 2011-08-10 2013-02-14 Novozymes A/S Polypeptides having peroxygenase activity and polynucleotides encoding same
WO2013021062A1 (en) 2011-08-10 2013-02-14 Novozymes A/S Polypeptides having peroxygenase activity and polynucleotides encoding same
WO2013021065A1 (en) 2011-08-10 2013-02-14 Novozymes A/S Polypeptides having peroxygenase activity and polynucleotides encoding same
WO2013021059A1 (en) 2011-08-10 2013-02-14 Novozymes A/S Polypeptides having peroxygenase activity and polynucleotides encoding same
US9487761B2 (en) 2011-08-10 2016-11-08 Novozymes A/S Polypeptides having peroxygenase activity and polynucleotides encoding same
WO2013079531A2 (en) 2011-12-02 2013-06-06 Novozymes A/S Polypeptides having peroxygenase activity and polynucleotides encoding same
US9404093B2 (en) 2011-12-02 2016-08-02 Novozymes A/S Polypeptides having peroxygenase activity and polynucleotides encoding same
JP2015516804A (ja) 2012-03-31 2015-06-18 ノボザイムス アクティーゼルスカブ ペルオキシゲナーゼを用いたエポキシ化
PT2875179T (pt) 2012-07-18 2018-04-23 Novozymes As Resumo
US20150152452A1 (en) 2012-07-20 2015-06-04 Novozymes A/S Enzymatic Oxidation of 5-Hydroxymethylfurfural and Derivatives Thereof
CN104718287B (zh) * 2012-10-12 2018-02-06 诺维信公司 具有过氧合酶活性的多肽
WO2014056927A2 (en) 2012-10-12 2014-04-17 Novozymes A/S Polypeptides having peroxygenase activity
WO2014056922A2 (en) 2012-10-12 2014-04-17 Novozymes A/S Polypeptides having peroxygenase activity
WO2014056920A2 (en) 2012-10-12 2014-04-17 Novozymes A/S Polypeptides having peroxygenase activity
CN104704116B (zh) * 2012-10-12 2018-09-28 诺维信公司 具有过氧合酶活性的多肽
US9499802B2 (en) 2012-10-12 2016-11-22 Novozymes A/S Polypeptides having peroxygenase activity
US9458435B2 (en) 2012-10-12 2016-10-04 Novozymes A/S Polypeptides having peroxygenase activity
EP2906685B1 (en) 2012-10-12 2018-08-15 Novozymes A/S Polypeptides having peroxygenase activity
WO2014090940A1 (en) * 2012-12-14 2014-06-19 Novozymes A/S Removal of skin-derived body soils
EP2871233A1 (de) 2013-11-12 2015-05-13 Brandenburgische Technische Universität Cottbus-Senftenberg Verfahren zur Herstellung von biogenen Stoffen
JP6291051B2 (ja) * 2013-11-18 2018-03-14 エンザイマティック デインキング テクノロジーズ， エルエルシー 製紙用バージン繊維及び再生紙の残存鉱油濃度低減のための酵素処理
US10465172B2 (en) 2013-11-29 2019-11-05 Novozymes A/S Peroxygenase variants
WO2016207373A1 (en) 2015-06-26 2016-12-29 Novozymes A/S Polypeptides having peroxygenase activity
ES2869296T3 (es) 2017-04-20 2021-10-25 Consejo Superior Investigacion Proceso para acortar la cadena hidrocarbonada de un ácido carboxílico mediante una peroxidasa
NL2021113B1 (en) 2018-06-13 2019-12-19 Univ Delft Tech Synthesis of aromatic epoxide derived compounds
US11866757B2 (en) 2018-07-03 2024-01-09 Ruhr-Universitaet Bochum Plasma-driven biocatalysis
EP3594332A1 (en) * 2018-07-10 2020-01-15 Consejo Superior de Investigaciones Cientificas Method of heterologous expression of active fungal unspecific peroxygenase in bacterial host cells for fatty-acid epoxidation and other oxygenation reactions
EP3660147A1 (en) 2018-11-30 2020-06-03 Consejo Superior de Investigaciones Cientificas (CSIC) Process for selective synthesis of 4-hydroxyisophorone and 4-ketoisophorone by fungal peroxygenases
US20220064516A1 (en) * 2019-01-03 2022-03-03 Locus Oil Ip Company, Llc Materials and Methods for Extended Reduction of Heavy Crude Oil Viscosity
CN114641565B (zh) 2019-07-05 2024-10-22 比西有限公司 重组血红素硫醇盐加氧酶
US20240010996A1 (en) 2020-09-23 2024-01-11 Gecco Biotech B.V. Bacterial unspecific peroxygenases (BUPO's) and methods and uses thereof
DE102021204094A1 (de) 2021-04-25 2022-10-27 Brandenburgische Technische Universität Cottbus-Senftenberg In-vitro Arzneimittel aus Prodrugs und deren Verwendung
DE102021209758A1 (de) 2021-09-03 2023-03-09 Brandenburgische Technische Universität Cottbus-Senftenberg Verfahren zur zellfreien Herstellung von unspezifischen Peroxygenasen und deren Verwendung
DE102021214582A1 (de) 2021-12-17 2023-06-22 Brandenburgische Technische Universität Cottbus-Senftenberg Verfahren zur zellfreien Herstellung von unspezifischen Peroxygenasen und deren Verwendung
EP4396194A1 (de) 2021-09-03 2024-07-10 Brandenburgische Technische Universität Cottbus-Senftenberg Verfahren zur zellfreien herstellung von unspezifischen peroxygenasen
CN116240246B (zh) * 2021-12-20 2025-01-21 浙江金朗博药业有限公司 利用过氧化物酶合成骨化二醇的方法

Family Cites Families (73)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1296839A (enExample) 1969-05-29 1972-11-22
GB1372034A (en) 1970-12-31 1974-10-30 Unilever Ltd Detergent compositions
US3826746A (en) 1972-07-18 1974-07-30 Mobil Oil Corp Lubricant compositions
GB1483591A (en) 1973-07-23 1977-08-24 Novo Industri As Process for coating water soluble or water dispersible particles by means of the fluid bed technique
GB1590432A (en) 1976-07-07 1981-06-03 Novo Industri As Process for the production of an enzyme granulate and the enzyme granuate thus produced
DK187280A (da) 1980-04-30 1981-10-31 Novo Industri As Ruhedsreducerende middel til et fuldvaskemiddel fuldvaskemiddel og fuldvaskemetode
US4353985A (en) 1980-07-14 1982-10-12 Schering Aktiengesellschaft Process for the preparation of 11 β-hydroxy steroids
DK263584D0 (da) 1984-05-29 1984-05-29 Novo Industri As Enzymholdige granulater anvendt som detergentadditiver
US4933287A (en) 1985-08-09 1990-06-12 Gist-Brocades N.V. Novel lipolytic enzymes and their use in detergent compositions
EG18543A (en) 1986-02-20 1993-07-30 Albright & Wilson Protected enzyme systems
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
US4810414A (en) 1986-08-29 1989-03-07 Novo Industri A/S Enzymatic detergent additive
NZ221627A (en) 1986-09-09 1993-04-28 Genencor Inc Preparation of enzymes, modifications, catalytic triads to alter ratios or transesterification/hydrolysis ratios
EP0305216B1 (en) 1987-08-28 1995-08-02 Novo Nordisk A/S Recombinant Humicola lipase and process for the production of recombinant humicola lipases
JPS6474992A (en) 1987-09-16 1989-03-20 Fuji Oil Co Ltd Dna sequence, plasmid and production of lipase
DE68924654T2 (de) 1988-01-07 1996-04-04 Novonordisk As Spezifische Protease.
DK6488D0 (da) 1988-01-07 1988-01-07 Novo Industri As Enzymer
JP3079276B2 (ja) 1988-02-28 2000-08-21 天野製薬株式会社 組換え体ｄｎａ、それを含むシュードモナス属菌及びそれを用いたリパーゼの製造法
WO1989009259A1 (en) 1988-03-24 1989-10-05 Novo-Nordisk A/S A cellulase preparation
US5648263A (en) 1988-03-24 1997-07-15 Novo Nordisk A/S Methods for reducing the harshness of a cotton-containing fabric
US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
GB8915658D0 (en) 1989-07-07 1989-08-23 Unilever Plc Enzymes,their production and use
NZ237549A (en) 1990-03-23 1993-06-25 Gist Brocades Nv Production of enhanced levels of enzymes in the seeds of transgenic plants and the use of these seeds
WO1991016422A1 (de) 1990-04-14 1991-10-31 Kali-Chemie Aktiengesellschaft Alkalische bacillus-lipasen, hierfür codierende dna-sequenzen sowie bacilli, die diese lipasen produzieren
WO1991017243A1 (en) 1990-05-09 1991-11-14 Novo Nordisk A/S A cellulase preparation comprising an endoglucanase enzyme
DK115890D0 (da) 1990-05-09 1990-05-09 Novo Nordisk As Enzym
BR9106839A (pt) 1990-09-13 1993-07-20 Novo Nordisk As Variante de lipase,construcao de dna,vetor de expressao de recombinante,celula,planta,processo para produzir uma variante de lipase,aditivo e composicao de detergente
IL99552A0 (en) 1990-09-28 1992-08-18 Ixsys Inc Compositions containing procaryotic cells,a kit for the preparation of vectors useful for the coexpression of two or more dna sequences and methods for the use thereof
DE69133035T2 (de) 1991-01-16 2003-02-13 The Procter & Gamble Company, Cincinnati Kompakte Waschmittelzusammensetzungen mit hochaktiven Cellulasen
SK120893A3 (en) 1991-04-30 1994-08-10 Procter & Gamble Liquid detergent mixtures with boric-polyol complex for inhibition of proteolytic enzyme
EP0511456A1 (en) 1991-04-30 1992-11-04 The Procter & Gamble Company Liquid detergents with aromatic borate ester to inhibit proteolytic enzyme
EP0583339B1 (en) 1991-05-01 1998-07-08 Novo Nordisk A/S Stabilized enzymes and detergent compositions
DK72992D0 (da) 1992-06-01 1992-06-01 Novo Nordisk As Enzym
DK88892D0 (da) 1992-07-06 1992-07-06 Novo Nordisk As Forbindelse
KR100294361B1 (ko) 1992-07-23 2001-09-17 피아 스타르 돌연변이체알파-아밀라제,세정제,접시세척제,및액화제
KR100303619B1 (ko) 1992-10-06 2001-11-22 피아 스타르 셀룰라제변이체
KR100322793B1 (ko) 1993-02-11 2002-06-20 마가렛 에이.혼 산화안정성알파-아밀라아제
DK0652946T3 (da) 1993-04-27 2005-05-30 Genencor Int Nye lipase-varianter til anvendelse i detergenter
DK52393D0 (enExample) 1993-05-05 1993-05-05 Novo Nordisk As
JP2859520B2 (ja) 1993-08-30 1999-02-17 ノボ ノルディスク アクティーゼルスカブ リパーゼ及びそれを生産する微生物及びリパーゼ製造方法及びリパーゼ含有洗剤組成物
CA2173946A1 (en) 1993-10-13 1995-04-20 Anders Hjelholt Pedersen H2o2-stable peroxidase variants
JPH07143883A (ja) 1993-11-24 1995-06-06 Showa Denko Kk リパーゼ遺伝子及び変異体リパーゼ
DE4343591A1 (de) 1993-12-21 1995-06-22 Evotec Biosystems Gmbh Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
WO1995022615A1 (en) 1994-02-22 1995-08-24 Novo Nordisk A/S A method of preparing a variant of a lipolytic enzyme
ES2251717T3 (es) 1994-03-08 2006-05-01 Novozymes A/S Nuevas celulasas alcalinas.
AU2524695A (en) 1994-05-04 1995-11-29 Genencor International, Inc. Lipases with improved surfactant resistance
CN1192108C (zh) 1994-06-03 2005-03-09 诺沃奇梅兹生物技术有限公司 纯化的毁丝霉属漆酶及编码该酶的核酸
WO1995035381A1 (en) 1994-06-20 1995-12-28 Unilever N.V. Modified pseudomonas lipases and their use
WO1996000292A1 (en) 1994-06-23 1996-01-04 Unilever N.V. Modified pseudomonas lipases and their use
WO1996000787A1 (en) 1994-06-30 1996-01-11 Novo Nordisk Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein
US5919691A (en) 1994-10-06 1999-07-06 Novo Nordisk A/S Enzyme and enzyme preparation with endoglucanase activity
BE1008998A3 (fr) 1994-10-14 1996-10-01 Solvay Lipase, microorganisme la produisant, procede de preparation de cette lipase et utilisations de celle-ci.
CA2203398A1 (en) 1994-10-26 1996-05-09 Thomas Sandal An enzyme with lipolytic activity
AR000862A1 (es) 1995-02-03 1997-08-06 Novozymes As Variantes de una ó-amilasa madre, un metodo para producir la misma, una estructura de adn y un vector de expresion, una celula transformada por dichaestructura de adn y vector, un aditivo para detergente, composicion detergente, una composicion para lavado de ropa y una composicion para la eliminacion del
JPH08228778A (ja) 1995-02-27 1996-09-10 Showa Denko Kk 新規なリパーゼ遺伝子及びそれを用いたリパーゼの製造方法
BRPI9607646B1 (pt) 1995-03-17 2016-07-05 Novo Nordisk As vetor de expressão recombinante, célula fúngica, método para produzir uma enzima apresentando atividade de endoglucanase e para prover clarificação da cor nas roupas de lavagem, composição de lavanderia , uso da enzima, e, composição de enzima
DE69633825T2 (de) 1995-07-14 2005-11-10 Novozymes A/S Modifiziertes enzym mit lipolytischer aktivität
EP0851913B1 (en) 1995-08-11 2004-05-19 Novozymes A/S Novel lipolytic enzymes
US5763385A (en) 1996-05-14 1998-06-09 Genencor International, Inc. Modified α-amylases having altered calcium binding properties
AU3938997A (en) 1996-08-26 1998-03-19 Novo Nordisk A/S A novel endoglucanase
EP0937138B1 (en) 1996-09-17 2006-04-26 Novozymes A/S Cellulase variants
CA2265734A1 (en) 1996-10-08 1998-04-16 Novo Nordisk A/S Diaminobenzoic acid derivatives as dye precursors
ATE510910T1 (de) 1996-11-04 2011-06-15 Novozymes As Subtilase-varianten und verbindungen
JP2001503269A (ja) 1996-11-04 2001-03-13 ノボ ノルディスク アクティーゼルスカブ ズブチラーゼ変異体及び、組成物
WO1998034946A1 (en) 1997-02-12 1998-08-13 Massachusetts Institute Of Technology Daxx, a novel fas-binding protein that activates jnk and apoptosis
AU7908898A (en) 1997-07-04 1999-01-25 Novo Nordisk A/S Family 6 endo-1,4-beta-glucanase variants and cleaning composit ions containing them
CN1197965C (zh) 1998-10-26 2005-04-20 诺维信公司 在丝状真菌细胞内构建和筛选目的dna文库
AU4025700A (en) 1999-03-22 2000-10-09 Novo Nordisk Biotech, Inc. Promoters for expressing genes in a fungal cell
DE10332065A1 (de) 2003-07-11 2005-01-27 Friedrich-Schiller-Universität Jena Verfahren zur enzymatischen Darstellung von Säuren aus Alkoholen über die intermediäre Bildung von Aldehyden
ES2332592T3 (es) 2003-10-30 2010-02-09 Novozymes A/S Modulos de union a carbohidratos.
DE102004047774A1 (de) 2004-09-28 2006-03-30 Jenabios Gmbh Verfahren zur enzymatischen Hydroxylierung nicht-aktivierter Kohlenwasserstoffe
WO2008016709A2 (en) * 2006-08-04 2008-02-07 California Institute Of Technology Methods and systems for selective fluorination of organic molecules

Also Published As

Publication number Publication date
CA2682451A1 (en) 2008-10-09
CA2682451C (en) 2015-11-24
US20130115662A1 (en) 2013-05-09
JP2010523079A (ja) 2010-07-15
EP2471911A2 (en) 2012-07-04
EP2158313A2 (en) 2010-03-03
CN101802180A (zh) 2010-08-11
US8367387B2 (en) 2013-02-05
EP2158313B1 (en) 2012-03-07
JP2014000086A (ja) 2014-01-09
CN101802180B (zh) 2017-04-05
EP2471911A3 (en) 2012-12-12
DK2158313T3 (da) 2012-05-14
US20100279366A1 (en) 2010-11-04
EP2468852A1 (en) 2012-06-27
EP2468852B1 (en) 2014-07-16
WO2008119780A2 (en) 2008-10-09
JP5411121B2 (ja) 2014-02-12
DE102007016139A1 (de) 2008-10-02
WO2008119780A3 (en) 2009-03-12

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