ATE502107T1 - Verfahren zur herstellung rekombinanter saccharose-synthase, verwendung davon bei der herstellung von kits zur saccharosebestimmung, verfahren zur herstellung von adp-glucose sowie verfahren zur erhaltung transgener pflanzen mit blättern und speicherorganen, in denen sich adp- glucose und stärke in hoher konzentration anreichern - Google Patents
Verfahren zur herstellung rekombinanter saccharose-synthase, verwendung davon bei der herstellung von kits zur saccharosebestimmung, verfahren zur herstellung von adp-glucose sowie verfahren zur erhaltung transgener pflanzen mit blättern und speicherorganen, in denen sich adp- glucose und stärke in hoher konzentration anreichernInfo
- Publication number
- ATE502107T1 ATE502107T1 AT05708108T AT05708108T ATE502107T1 AT E502107 T1 ATE502107 T1 AT E502107T1 AT 05708108 T AT05708108 T AT 05708108T AT 05708108 T AT05708108 T AT 05708108T AT E502107 T1 ATE502107 T1 AT E502107T1
- Authority
- AT
- Austria
- Prior art keywords
- production
- succarose
- glucose
- adp
- leaves
- Prior art date
Links
- 238000004519 manufacturing process Methods 0.000 title abstract 12
- 238000000034 method Methods 0.000 title abstract 4
- 238000003860 storage Methods 0.000 title abstract 4
- 229920002472 Starch Polymers 0.000 title abstract 3
- 210000000056 organ Anatomy 0.000 title abstract 3
- 239000008107 starch Substances 0.000 title abstract 3
- 235000019698 starch Nutrition 0.000 title abstract 3
- 230000009261 transgenic effect Effects 0.000 title abstract 3
- 239000008103 glucose Substances 0.000 title 2
- WFPZSXYXPSUOPY-ROYWQJLOSA-N ADP alpha-D-glucoside Chemical compound C([C@H]1O[C@H]([C@@H]([C@@H]1O)O)N1C=2N=CN=C(C=2N=C1)N)OP(O)(=O)OP(O)(=O)O[C@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O WFPZSXYXPSUOPY-ROYWQJLOSA-N 0.000 abstract 5
- 101710096830 DNA-3-methyladenine glycosylase Proteins 0.000 abstract 3
- 102100039128 DNA-3-methyladenine glycosylase Human genes 0.000 abstract 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 abstract 3
- 229930006000 Sucrose Natural products 0.000 abstract 3
- 108090000623 proteins and genes Proteins 0.000 abstract 3
- 239000005720 sucrose Substances 0.000 abstract 3
- 241000588724 Escherichia coli Species 0.000 abstract 1
- 108010029485 Protein Isoforms Proteins 0.000 abstract 1
- 102000001708 Protein Isoforms Human genes 0.000 abstract 1
- 108010043934 Sucrose synthase Proteins 0.000 abstract 1
- HSCJRCZFDFQWRP-JZMIEXBBSA-N UDP-alpha-D-glucose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1OP(O)(=O)OP(O)(=O)OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C(NC(=O)C=C2)=O)O1 HSCJRCZFDFQWRP-JZMIEXBBSA-N 0.000 abstract 1
- HSCJRCZFDFQWRP-UHFFFAOYSA-N Uridindiphosphoglukose Natural products OC1C(O)C(O)C(CO)OC1OP(O)(=O)OP(O)(=O)OCC1C(O)C(O)C(N2C(NC(=O)C=C2)=O)O1 HSCJRCZFDFQWRP-UHFFFAOYSA-N 0.000 abstract 1
- NBSCHQHZLSJFNQ-DVKNGEFBSA-N alpha-D-glucose 6-phosphate Chemical compound O[C@H]1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H](O)[C@H]1O NBSCHQHZLSJFNQ-DVKNGEFBSA-N 0.000 abstract 1
- 238000003149 assay kit Methods 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 239000013604 expression vector Substances 0.000 abstract 1
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
- C12N9/1062—Sucrose synthase (2.4.1.13)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/91—Transferases (2.)
- G01N2333/91091—Glycosyltransferases (2.4)
- G01N2333/91097—Hexosyltransferases (general) (2.4.1)
- G01N2333/91102—Hexosyltransferases (general) (2.4.1) with definite EC number (2.4.1.-)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES200400257A ES2245867B1 (es) | 2004-02-05 | 2004-02-05 | Procedimiento produccion sacarosa sintasa recombinante a partir de escherichia coli y uso en fabricacion de kits de determinacion de sacarosa, produccion de azucares-nucleotidos y obtencion de plantas transgenicas con alto contenido de almidon y alto balance amilosa/amilopectina. |
| PCT/ES2005/070010 WO2005075649A1 (es) | 2004-02-05 | 2005-01-27 | Procedimiento para la producción de sacarosa sintasa recombinante su uso en la fabricación de kits de determinación de sacarosa producción de adpglucosa y obtención de plantas transgénicas cuyas hojas y órganos de reserva acumulen alto contenido en adpglucosa y almidón |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE502107T1 true ATE502107T1 (de) | 2011-04-15 |
Family
ID=34833892
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT05708108T ATE502107T1 (de) | 2004-02-05 | 2005-01-27 | Verfahren zur herstellung rekombinanter saccharose-synthase, verwendung davon bei der herstellung von kits zur saccharosebestimmung, verfahren zur herstellung von adp-glucose sowie verfahren zur erhaltung transgener pflanzen mit blättern und speicherorganen, in denen sich adp- glucose und stärke in hoher konzentration anreichern |
Country Status (17)
| Country | Link |
|---|---|
| US (3) | USRE46642E1 (de) |
| EP (1) | EP1721980B1 (de) |
| JP (1) | JP4738351B2 (de) |
| KR (1) | KR101244638B1 (de) |
| CN (1) | CN1984994B (de) |
| AT (1) | ATE502107T1 (de) |
| AU (1) | AU2005210055B2 (de) |
| BR (1) | BRPI0507477B1 (de) |
| CA (2) | CA3002836C (de) |
| DE (1) | DE602005026917D1 (de) |
| ES (3) | ES2245867B1 (de) |
| IL (1) | IL176847A (de) |
| MX (1) | MXPA06008811A (de) |
| PL (1) | PL1721980T3 (de) |
| RU (1) | RU2369637C2 (de) |
| WO (1) | WO2005075649A1 (de) |
| ZA (1) | ZA200606235B (de) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2354895B1 (es) * | 2008-09-12 | 2012-01-23 | Iden Carbohydrate Biotechnology, S.L | Procedimiento para la producción de plantas transgénicas que presentan alto contenido y rendimiento en almidón y alto balance amilosa/amilopectina. |
| ES2339094B1 (es) * | 2008-11-13 | 2011-03-18 | Iden Carbohydrate Biotechnology, S.L. | Procedimiento para la produccion de plantas transgenicas que presentan alto contenido en compuestos antioxidantes, alta capacidad antioxidante y resistencia al empardecimiento. |
| CN106834343B (zh) * | 2017-02-21 | 2019-09-13 | 中国农业大学 | 蔗糖合成酶在调控植物果实发育中的应用 |
| KR101985668B1 (ko) * | 2017-10-30 | 2019-06-04 | 명지대학교산학협력단 | 벼 유래 OsSUS4 유전자를 이용한 도열병 저항성이 조절된 형질전환 식물체의 제조방법 및 그에 따른 식물체 |
| CN111172237A (zh) * | 2019-10-24 | 2020-05-19 | 新疆农业科学院农产品贮藏加工研究所 | 一种测定植物中蔗糖合成酶和蔗糖磷酸合成酶含量的方法 |
| DE102020111560A1 (de) | 2020-04-28 | 2021-10-28 | Rheinisch-Westfälische Technische Hochschule (Rwth) Aachen | Enzymkaskaden auf Basis von Saccharose Synthase und Pyrophosphorylase zur Umsetzung von ADP zu ATP |
| CN111793641B (zh) * | 2020-07-20 | 2022-07-19 | 中国农业科学院郑州果树研究所 | 甜樱桃PavSS或PavSPS基因在调控果实着色或果实成熟软化中的用途 |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1340522C (en) * | 1987-03-10 | 1999-05-04 | Heinz Dobeli | Fusion proteins containing neighbouring histidines for improved purification |
| WO1994028146A2 (en) * | 1993-05-24 | 1994-12-08 | Hoechst Schering Agrevo Gmbh | Dna sequences and plasmids for the preparation of sugar beet with changed sucrose concentration |
| US6682918B1 (en) * | 1996-07-19 | 2004-01-27 | Arch Development Corporation | Bacterial sucrose synthase compositions and methods of use |
| DE19736343B4 (de) * | 1997-08-21 | 2006-02-09 | Forschungszentrum Jülich GmbH | Verfahren zur Erhöhung der Genexpression von Saccharose Synthase |
| US7598361B2 (en) * | 1997-11-24 | 2009-10-06 | Monsanto Technology Llc | Nucleic acid molecules and other molecules associated with the sucrose pathway |
| JP2001335600A (ja) * | 2000-05-25 | 2001-12-04 | Funakoshi Co Ltd | タンパク質の精製方法 |
| JP2002065265A (ja) * | 2000-08-25 | 2002-03-05 | Sumitomo Electric Ind Ltd | 複合タグ |
| EP1349446B1 (de) * | 2000-12-08 | 2013-01-23 | Commonwealth Scientific And Industrial Research Organisation | Modifikation der saccharosesynthase-genexpression in pflanzengewebe und ihre verwendung |
| MXPA03007469A (es) * | 2001-02-22 | 2004-07-30 | Pioneer Hi Bred Int | Manipulacion de genes sucrosa sintasa para mejorar la calidad del grano y tallo. |
-
2004
- 2004-02-05 ES ES200400257A patent/ES2245867B1/es not_active Expired - Lifetime
-
2005
- 2005-01-27 EP EP05708108A patent/EP1721980B1/de not_active Not-in-force
- 2005-01-27 JP JP2006551865A patent/JP4738351B2/ja not_active Expired - Fee Related
- 2005-01-27 CN CN200580004166XA patent/CN1984994B/zh not_active Expired - Fee Related
- 2005-01-27 WO PCT/ES2005/070010 patent/WO2005075649A1/es active Application Filing
- 2005-01-27 ES ES05708108T patent/ES2363231T3/es active Active
- 2005-01-27 CA CA3002836A patent/CA3002836C/en not_active Expired - Fee Related
- 2005-01-27 AT AT05708108T patent/ATE502107T1/de not_active IP Right Cessation
- 2005-01-27 RU RU2006131670/13A patent/RU2369637C2/ru not_active IP Right Cessation
- 2005-01-27 KR KR1020067015794A patent/KR101244638B1/ko not_active IP Right Cessation
- 2005-01-27 AU AU2005210055A patent/AU2005210055B2/en not_active Ceased
- 2005-01-27 DE DE602005026917T patent/DE602005026917D1/de active Active
- 2005-01-27 PL PL05708108T patent/PL1721980T3/pl unknown
- 2005-01-27 MX MXPA06008811A patent/MXPA06008811A/es active IP Right Grant
- 2005-01-27 BR BRPI0507477-0A patent/BRPI0507477B1/pt not_active IP Right Cessation
- 2005-01-27 CA CA2554780A patent/CA2554780C/en not_active Expired - Fee Related
- 2005-01-27 US US14/267,538 patent/USRE46642E1/en not_active Expired - Fee Related
- 2005-01-27 US US10/587,372 patent/US8168856B2/en not_active Ceased
-
2006
- 2006-07-13 IL IL176847A patent/IL176847A/en not_active IP Right Cessation
- 2006-07-27 ZA ZA200606235A patent/ZA200606235B/xx unknown
-
2007
- 2007-12-21 ES ES200703413A patent/ES2342246B1/es active Active
-
2012
- 2012-04-27 US US13/458,228 patent/US8841514B2/en not_active Expired - Fee Related
Also Published As
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| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |