ATE487795T1 - Für die herstellung von polyhydroxyalkanoaten verwendbare dns-sequenz - Google Patents
Für die herstellung von polyhydroxyalkanoaten verwendbare dns-sequenzInfo
- Publication number
- ATE487795T1 ATE487795T1 AT97937273T AT97937273T ATE487795T1 AT E487795 T1 ATE487795 T1 AT E487795T1 AT 97937273 T AT97937273 T AT 97937273T AT 97937273 T AT97937273 T AT 97937273T AT E487795 T1 ATE487795 T1 AT E487795T1
- Authority
- AT
- Austria
- Prior art keywords
- fatty acid
- phag
- pha
- production
- biosynthesis
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/62—Carboxylic acid esters
- C12P7/625—Polyesters of hydroxy carboxylic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1025—Acyltransferases (2.3)
- C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Nutrition Science (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Plant Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Polyesters Or Polycarbonates (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/700,576 US5750848A (en) | 1996-08-13 | 1996-08-13 | DNA sequence useful for the production of polyhydroxyalkanoates |
| PCT/US1997/014388 WO1998006854A1 (en) | 1996-08-13 | 1997-08-13 | Dna sequence useful for the production of polyhydroxyalkanoates |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE487795T1 true ATE487795T1 (de) | 2010-11-15 |
Family
ID=24814040
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT97937273T ATE487795T1 (de) | 1996-08-13 | 1997-08-13 | Für die herstellung von polyhydroxyalkanoaten verwendbare dns-sequenz |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US5750848A (de) |
| EP (1) | EP0920517B1 (de) |
| JP (2) | JP2000516813A (de) |
| AT (1) | ATE487795T1 (de) |
| AU (1) | AU3982897A (de) |
| DE (1) | DE69740049D1 (de) |
| WO (1) | WO1998006854A1 (de) |
Families Citing this family (87)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5750848A (en) * | 1996-08-13 | 1998-05-12 | Monsanto Company | DNA sequence useful for the production of polyhydroxyalkanoates |
| US6117658A (en) * | 1997-02-13 | 2000-09-12 | James Madison University | Methods of making polyhydroxyalkanoates comprising 4-hydroxybutyrate monomer units |
| EP1060250A1 (de) * | 1998-03-06 | 2000-12-20 | Metabolix, Inc. | Veränderung des fettsäurestoffwechsels in pflanzen |
| US6586658B1 (en) | 1998-03-06 | 2003-07-01 | Metabolix, Inc. | Modification of fatty acid metabolism in plants |
| US7041875B1 (en) * | 1998-03-18 | 2006-05-09 | Eduardo Blumwald | Genetic engineering salt tolerance in crop plants |
| US7442852B2 (en) | 1998-03-18 | 2008-10-28 | Eduardo Blumwald | High salt plants and uses for bioremediation |
| US6936750B2 (en) * | 1998-03-18 | 2005-08-30 | Eduardo Blumwald | Increasing salt tolerance in plants by overexpression of a vacuolar Na+/H+ transporter[s] |
| WO1999061624A2 (en) | 1998-05-22 | 1999-12-02 | Metabolix, Inc. | Polyhydroxyalkanoate biopolymer compositions |
| DE69929700T2 (de) * | 1998-07-30 | 2006-08-31 | Metabolix, Inc., Cambridge | Bifunktionelle enzyme zur biopolymerherstellung |
| EP1700909B1 (de) * | 1998-08-18 | 2015-08-12 | Metabolix, Inc. | Transgene Polyhydroxyalkanoat produzierende Mikroben |
| CA2369590C (en) | 1998-12-22 | 2008-02-26 | National Institute Of Agrobiological Sciences | Sodium/proton antiporter gene |
| PT1161539E (pt) | 1999-03-15 | 2005-09-30 | Univ Laval | Metodo para produzir polihidroxialcanoatos em organismos recombinantes |
| EP1196614A1 (de) * | 1999-06-24 | 2002-04-17 | Metabolix, Inc. | Pflanzliche multigen-expressionskonstrukte |
| DE19935088A1 (de) * | 1999-07-27 | 2001-02-01 | Inst Genomic Res Tigr Rockvill | DNA-Sequenzen, die sich zum spezifischen Nachweis von Pseudomonas putida KT2440 eignen |
| US20020199218A1 (en) * | 1999-08-19 | 2002-12-26 | Daphne Goring | Proline-rich extensin-like receptor kinases |
| ATE454456T1 (de) | 2000-02-11 | 2010-01-15 | Metabolix Inc | Intein enthaltende multigen-expressionskonstrukte |
| JP2001275677A (ja) * | 2000-03-30 | 2001-10-09 | Canon Inc | 核酸断片プライマーまたはプローブ、およびこれを用いたポリヒドロキシアルカノエート合成微生物の検出方法 |
| US7786355B2 (en) * | 2000-11-17 | 2010-08-31 | Metabolix, Inc. | Production of medium chain length polyhydroxyalkanoates from fatty acid biosynthetic pathways |
| CA2364983A1 (en) * | 2000-12-13 | 2002-06-13 | John R. Coleman | Nucleic acid molecules and polypeptides for catabolism of abscisic acid |
| WO2003026417A2 (en) * | 2001-09-24 | 2003-04-03 | Prodigene, Inc. | Method of increasing expression of heterologous proteins in plants |
| EP1457554B1 (de) * | 2001-12-10 | 2008-08-20 | Riken | Verfahren zur herstellung von biologisch abbaubarem polyester |
| KR100439123B1 (ko) * | 2002-05-14 | 2004-07-05 | 윤성철 | 폴리히드록시알칸산-생산균주 및 그를 이용한폴리히드록시알칸산의 생산방법 |
| US20050034191A1 (en) * | 2002-07-12 | 2005-02-10 | Eduardo Blumwald | Salt tolerant oil crops |
| ATE516295T1 (de) | 2004-01-20 | 2011-07-15 | Monsanto Technology Llc | Chimäre promotoren zur verwendung in pflanzen |
| US20060200878A1 (en) * | 2004-12-21 | 2006-09-07 | Linda Lutfiyya | Recombinant DNA constructs and methods for controlling gene expression |
| US8314290B2 (en) | 2004-12-21 | 2012-11-20 | Monsanto Technology Llc | Temporal regulation of gene expression by MicroRNAs |
| EP2765189A1 (de) | 2004-12-21 | 2014-08-13 | Monsanto Technology LLC | Rekombinante DNA-Konstrukte und Verfahren zur Steuerung der Gen-Expression |
| JP2008532553A (ja) * | 2005-03-16 | 2008-08-21 | メタボリックス, インコーポレイテッド | 生合成経路の化学的に誘導可能な発現 |
| AR053279A1 (es) * | 2005-05-19 | 2007-04-25 | Monsanto Technology Llc | Regulacion post-transcripcional de la expresion genica |
| WO2007047016A2 (en) | 2005-10-13 | 2007-04-26 | Monsanto Technology, Llc | Methods for producing hybrid seed |
| WO2008063203A2 (en) | 2006-01-27 | 2008-05-29 | Whitehead Institute For Biomedical Research | Compositions and methods for efficient gene silencing in plants |
| CN101421406B (zh) | 2006-02-13 | 2016-08-31 | 孟山都技术有限公司 | 用于产生改变的种子油组成的核酸构建体和方法 |
| EP2371964A1 (de) | 2006-05-16 | 2011-10-05 | Monsanto Technology LLC | Verwendung einer nichtagrobakteriellen Spezies zur Pflanzenumwandlung |
| CN101600798A (zh) | 2006-08-31 | 2009-12-09 | 孟山都技术有限公司 | 相位型小rna |
| EP3199646A1 (de) | 2006-10-12 | 2017-08-02 | Monsanto Technology LLC | Pflanzen-mikro-rnas und verfahren zur verwendung davon |
| US8410334B2 (en) | 2007-02-20 | 2013-04-02 | Monsanto Technology Llc | Invertebrate microRNAs |
| US7838729B2 (en) | 2007-02-26 | 2010-11-23 | Monsanto Technology Llc | Chloroplast transit peptides for efficient targeting of DMO and uses thereof |
| CN105219694A (zh) | 2007-03-09 | 2016-01-06 | 孟山都技术公司 | 用于转化的植物胚外植体的制备和用途 |
| JP5551448B2 (ja) | 2007-03-16 | 2014-07-16 | ジェノマティカ・インコーポレイテッド | 1,4−ブタンジオールおよびその前駆体の生合成のための組成物および方法 |
| US7947483B2 (en) | 2007-08-10 | 2011-05-24 | Genomatica, Inc. | Methods and organisms for the growth-coupled production of 1,4-butanediol |
| CN103333894B (zh) | 2008-04-07 | 2016-11-23 | 孟山都技术公司 | 植物调控元件及其应用 |
| AU2009291825B2 (en) | 2008-09-10 | 2016-05-05 | Genomatica, Inc. | Microorganisms for the production of 1,4-butanediol |
| JP5019545B2 (ja) | 2008-10-24 | 2012-09-05 | 日本特殊陶業株式会社 | セラミック接合体、セラミックヒータおよびガスセンサ |
| US20120277117A1 (en) | 2009-02-27 | 2012-11-01 | Adel Zayed | Hydroponic apparatus and methods of use |
| EP4056706A1 (de) | 2009-06-04 | 2022-09-14 | Genomatica, Inc. | Mikroorganismen zur herstellung von 1,4-butandiol und entsprechende verfahren |
| ES2625623T3 (es) * | 2009-06-04 | 2017-07-20 | Genomatica, Inc. | Procedimiento de separación de componentes de un caldo de fermentación |
| CN105441374A (zh) * | 2009-10-13 | 2016-03-30 | 基因组股份公司 | 生产1,4-丁二醇、4-羟基丁醛、4-羟基丁酰-coa、腐胺和相关化合物的微生物及其相关方法 |
| US8530210B2 (en) | 2009-11-25 | 2013-09-10 | Genomatica, Inc. | Microorganisms and methods for the coproduction 1,4-butanediol and gamma-butyrolactone |
| EP2524045B1 (de) | 2010-01-14 | 2015-12-02 | Monsanto Technology LLC | Pflanzenregulierungselemente und verwendungen davon |
| US8048661B2 (en) * | 2010-02-23 | 2011-11-01 | Genomatica, Inc. | Microbial organisms comprising exogenous nucleic acids encoding reductive TCA pathway enzymes |
| US8445244B2 (en) * | 2010-02-23 | 2013-05-21 | Genomatica, Inc. | Methods for increasing product yields |
| WO2012083137A1 (en) | 2010-12-17 | 2012-06-21 | Monsanto Technology Llc | Methods for improving competency of plant cells |
| MX356792B (es) | 2011-03-25 | 2018-06-12 | Monsanto Technology Llc | Elementos de regulacion de plantas y sus usos. |
| EA031636B1 (ru) | 2011-05-13 | 2019-01-31 | Монсанто Текнолоджи Ллс | Регуляторные элементы растений и их применение |
| EP2565265A1 (de) | 2011-09-02 | 2013-03-06 | Philip Morris Products S.A. | Isopropylmalat-Synthase aus Nicotiana Tabacum und Verfahren und Verwendungen davon |
| CN104411827B (zh) | 2012-02-29 | 2018-01-02 | 陶氏益农公司 | 甘蔗杆状病毒(scbv)增强子及其在植物功能基因组学中的用途 |
| US9663793B2 (en) | 2012-04-20 | 2017-05-30 | Monsanto Technology, Llc | Plant regulatory elements and uses thereof |
| US11932845B2 (en) | 2012-06-04 | 2024-03-19 | Genomatica, Inc. | Microorganisms and methods for production of 4-hydroxybutyrate, 1,4-butanediol and related compounds |
| KR102273360B1 (ko) | 2012-12-19 | 2021-07-06 | 몬산토 테크놀로지 엘엘씨 | 식물 조절 요소 및 그의 용도 |
| BR122020011572B1 (pt) | 2013-03-14 | 2021-10-13 | Monsanto Technology Llc | Cassete de expressão compreendendo elemento regulador de planta e método para produzir uma planta transgênica |
| JP2016514964A (ja) | 2013-03-14 | 2016-05-26 | モンサント テクノロジー エルエルシー | 植物調節エレメントおよびその使用 |
| US11519011B2 (en) | 2015-03-26 | 2022-12-06 | The Texas A&M University System | Conversion of lignin into bioplastics and lipid fuels |
| US11236347B2 (en) | 2015-08-28 | 2022-02-01 | Pioneer Hi-Bred International, Inc. | Ochrobactrum-mediated transformation of plants |
| US11732269B2 (en) | 2015-10-02 | 2023-08-22 | Monsanto Technology Llc | Recombinant maize B chromosome sequence and uses thereof |
| EP3426788B1 (de) | 2016-03-11 | 2021-09-08 | Monsanto Technology LLC | Pflanzenregulierungselemente und verwendungen davon |
| CA3029271A1 (en) | 2016-06-28 | 2018-01-04 | Cellectis | Altering expression of gene products in plants through targeted insertion of nucleic acid sequences |
| EP3475427A4 (de) | 2016-06-28 | 2019-11-06 | Monsanto Technology LLC | Verfahren und zusammensetzungen zur verwendung bei der genommodifizierung in pflanzen |
| WO2018136594A1 (en) | 2017-01-19 | 2018-07-26 | Monsanto Technology Llc | Plant regulatory elements and uses thereof |
| WO2019139616A1 (en) | 2018-01-12 | 2019-07-18 | The Texas A&M University System | Increasing plant bioproduct yield |
| BR112021003899A2 (pt) | 2019-01-10 | 2021-08-03 | Monsanto Technology Llc | elementos reguladores de plantas e seus usos |
| CN120718905A (zh) | 2019-05-29 | 2025-09-30 | 孟山都技术公司 | 使用基因组编辑产生显性等位基因的方法和组合物 |
| CN111849808A (zh) * | 2020-07-06 | 2020-10-30 | 北京大学深圳研究院 | 海洋源产pha菌富集培养及接种、pcr扩增基因方法 |
| WO2022204464A1 (en) | 2021-03-26 | 2022-09-29 | Flagship Pioneering Innovations Vii, Llc | Production of circular polyribonucleotides in a eukaryotic system |
| EP4314289A1 (de) | 2021-03-26 | 2024-02-07 | Flagship Pioneering Innovations VII, LLC | Herstellung von zirkulären polyribonukleotiden in einem prokaryotischen system |
| EP4314277A1 (de) | 2021-03-26 | 2024-02-07 | Flagship Pioneering Innovations VII, LLC | Zusammensetzungen und verfahren zur herstellung von zirkulären polyribonukleotiden |
| EP4426842A1 (de) | 2021-11-01 | 2024-09-11 | Flagship Pioneering Innovations VII, LLC | Polynukleotide zur modifizierung von organismen |
| AU2023337091A1 (en) | 2022-09-09 | 2025-03-06 | Eidgenössische Technische Hochschule Zürich | Plant regulatory elements and uses thereof |
| AR132588A1 (es) | 2023-05-03 | 2025-07-16 | Flagship Pioneering Innovations Vii Llc | Arn satélite de tymovirales artificiales |
| AR132586A1 (es) | 2023-05-03 | 2025-07-16 | Flagship Pioneering Innovations Vii Llc | Arn satélite de amalgavirus artificiales |
| CN121666453A (zh) | 2023-05-03 | 2026-03-13 | 旗舰创业创新第七有限责任公司 | 人工南方菜豆花叶病病毒科卫星rna |
| WO2024229351A1 (en) | 2023-05-03 | 2024-11-07 | Flagship Pioneering Innovations Vii, Llc | Artificial secoviridae satellite rnas |
| CN121666452A (zh) | 2023-05-03 | 2026-03-13 | 旗舰创业创新第七有限责任公司 | 人工马特利病毒目卫星rna |
| EP4705477A1 (de) | 2023-05-03 | 2026-03-11 | Flagship Pioneering Innovations VII, LLC | Endornavirale satelliten-rna-amplifikationssysteme für pflanzen |
| WO2024229395A1 (en) | 2023-05-03 | 2024-11-07 | Flagship Pioneering Innovations Vii, Llc | Partitiviral satellite rna amplification systems for plants |
| WO2024229385A1 (en) | 2023-05-03 | 2024-11-07 | Flagship Pioneering Innovations Vii, Llc | Artificial ghabrivirales satellite rnas |
| EP4705470A1 (de) | 2023-05-03 | 2026-03-11 | Flagship Pioneering Innovations VII, LLC | Künstliche tombusviridae-satelliten-rnas |
| US20250075226A1 (en) | 2023-08-29 | 2025-03-06 | University Of Freiburg | Proteins for regulation of symbiotic infection and associated regulatory elements |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL8603073A (nl) * | 1986-12-02 | 1988-07-01 | Rijksuniversiteit | Werkwijze voor het bereiden van polyesters door fermentatie; werkwijze voor het bereiden van optisch actieve carbonzuren en esters; polyester omvattende voortbrengselen. |
| US5480794A (en) * | 1987-06-29 | 1996-01-02 | Massachusetts Institute Of Technology And Metabolix, Inc. | Overproduction and purification of soluble PHA synthase |
| US5245023A (en) * | 1987-06-29 | 1993-09-14 | Massachusetts Institute Of Technology | Method for producing novel polyester biopolymers |
| NL8900827A (nl) * | 1989-04-04 | 1990-11-01 | Rijksuniversiteit | Microbiologische bereiding van polyesters. |
| KR100189468B1 (ko) * | 1991-04-09 | 1999-06-01 | 양갑석 | 폴리-베타-하이드록시알카노에이트(pha)공중합체및그제조방법,이를생산하는미생물과pha공중합체의고분자블렌드 |
| GB9108756D0 (en) * | 1991-04-24 | 1991-06-12 | Ici Plc | Production of polyalkanoate in plants |
| GB9115245D0 (en) * | 1991-07-16 | 1991-08-28 | Ici Plc | Production of polyalkanoate |
| US5610041A (en) * | 1991-07-19 | 1997-03-11 | Board Of Trustees Operating Michigan State University | Processes for producing polyhydroxybutyrate and related polyhydroxyalkanoates in the plastids of higher plants |
| US5569595A (en) * | 1991-09-27 | 1996-10-29 | Center For Innovative Technology | Production of poly-β-hydroxybutyrate in prokaryotic host cells |
| GB9223332D0 (en) * | 1992-11-06 | 1992-12-23 | Ici Plc | Production of polyhydroxyalkanoate in plants |
| US5750848A (en) * | 1996-08-13 | 1998-05-12 | Monsanto Company | DNA sequence useful for the production of polyhydroxyalkanoates |
| WO1998035044A1 (en) * | 1997-02-10 | 1998-08-13 | National Research Council Of Canada | Plant pyruvate dehydrogenase kinase gene |
-
1996
- 1996-08-13 US US08/700,576 patent/US5750848A/en not_active Ceased
-
1997
- 1997-08-13 JP JP10510057A patent/JP2000516813A/ja not_active Withdrawn
- 1997-08-13 AT AT97937273T patent/ATE487795T1/de not_active IP Right Cessation
- 1997-08-13 EP EP97937273A patent/EP0920517B1/de not_active Expired - Lifetime
- 1997-08-13 WO PCT/US1997/014388 patent/WO1998006854A1/en not_active Ceased
- 1997-08-13 DE DE69740049T patent/DE69740049D1/de not_active Expired - Lifetime
- 1997-08-13 AU AU39828/97A patent/AU3982897A/en not_active Abandoned
-
2000
- 2000-05-12 US US09/570,842 patent/USRE37543E1/en not_active Expired - Lifetime
-
2009
- 2009-03-13 JP JP2009060619A patent/JP2009225794A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| DE69740049D1 (de) | 2010-12-23 |
| EP0920517B1 (de) | 2010-11-10 |
| JP2009225794A (ja) | 2009-10-08 |
| USRE37543E1 (en) | 2002-02-05 |
| AU3982897A (en) | 1998-03-06 |
| WO1998006854A1 (en) | 1998-02-19 |
| US5750848A (en) | 1998-05-12 |
| EP0920517A1 (de) | 1999-06-09 |
| JP2000516813A (ja) | 2000-12-19 |
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