ATE479090T1 - Elektrophoreseverfahren mit paralleler und gleichzeitiger trennung - Google Patents

Elektrophoreseverfahren mit paralleler und gleichzeitiger trennung

Info

Publication number
ATE479090T1
ATE479090T1 AT06751738T AT06751738T ATE479090T1 AT E479090 T1 ATE479090 T1 AT E479090T1 AT 06751738 T AT06751738 T AT 06751738T AT 06751738 T AT06751738 T AT 06751738T AT E479090 T1 ATE479090 T1 AT E479090T1
Authority
AT
Austria
Prior art keywords
medium
separation
stabilization
anodic
cathodic
Prior art date
Application number
AT06751738T
Other languages
English (en)
Inventor
Gerhard Weber
Original Assignee
Becton Dickinson Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Becton Dickinson Co filed Critical Becton Dickinson Co
Application granted granted Critical
Publication of ATE479090T1 publication Critical patent/ATE479090T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44769—Continuous electrophoresis, i.e. the sample being continuously introduced, e.g. free flow electrophoresis [FFE]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44795—Isoelectric focusing

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Electrostatic Separation (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
AT06751738T 2005-04-29 2006-04-27 Elektrophoreseverfahren mit paralleler und gleichzeitiger trennung ATE479090T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102005020134A DE102005020134A1 (de) 2005-04-29 2005-04-29 Verfahren und Vorrichtung zur Durchführung eines parallelen und simultanen Mehrfachprozesses der trägerfreien isoelektrischen Fokussierung
PCT/US2006/016175 WO2006118996A2 (en) 2005-04-29 2006-04-27 Method for electrophoresis involving parallel and simultaneous separation

Publications (1)

Publication Number Publication Date
ATE479090T1 true ATE479090T1 (de) 2010-09-15

Family

ID=36955905

Family Applications (1)

Application Number Title Priority Date Filing Date
AT06751738T ATE479090T1 (de) 2005-04-29 2006-04-27 Elektrophoreseverfahren mit paralleler und gleichzeitiger trennung

Country Status (7)

Country Link
US (1) US8721861B2 (de)
EP (1) EP1877764B1 (de)
JP (1) JP4860693B2 (de)
CN (1) CN101184991B (de)
AT (1) ATE479090T1 (de)
DE (2) DE102005020134A1 (de)
WO (1) WO2006118996A2 (de)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080110758A1 (en) 2006-08-29 2008-05-15 Becton, Dickinson And Company Methods and apparatus for carrier-free deflection electrophoresis
EP2167951B1 (de) * 2007-06-20 2012-09-26 Becton, Dickinson and Company FFE-Verfahren unter Verwendung eines Trennmediums mit flüchtigem Puffer
WO2009133152A1 (en) * 2008-04-29 2009-11-05 Becton, Dickinson And Company Method and system for measuring a sample to determine the presence of and optionally treat a pathologic condition
JP2014524964A (ja) * 2011-07-28 2014-09-25 アンガス ケミカル カンパニー アミノアルコール化合物およびペイントおよび塗料用のゼロまたは低ｖｏｃ添加剤としてのそれらの使用
WO2014190434A1 (en) * 2013-05-29 2014-12-04 Ressources Geomega Inc. A system and method for separation and purification of dissolved rare earth/precious metals elements/compounds
WO2017132630A1 (en) * 2016-01-29 2017-08-03 Purigen Biosystems, Inc. Isotachophoresis for purification of nucleic acids
EP3662276B1 (de) * 2017-08-01 2024-07-10 Albert-Ludwigs-Universität Freiburg Elektrophoretische biomolekültrennvorrichtung
AU2018312570B2 (en) 2017-08-02 2024-01-11 Purigen Biosystems, Inc. Systems, devices, and methods for isotachophoresis
EP3474006A1 (de) * 2017-10-19 2019-04-24 Université de Liège Mikrochip für freiflusselektrophorese
EP4041427A4 (de) * 2019-10-09 2023-11-08 ProteinSimple Verfahren zur einkanaligen freifluss-elektrophorese mit sequenzieller ph-einstellung
US12084351B2 (en) 2021-06-30 2024-09-10 International Business Machines Corporation Carbon dioxide extraction using fluidic electrophoresis

Family Cites Families (90)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2555487A (en) 1948-02-27 1951-06-05 United Shoe Machinery Corp Chromatographic process and apparatus
DE805399C (de) 1949-05-25 1951-05-17 Dr Wolfgang Grassmann Verfahren und Vorrichtung zur Zerlegung von Stoffgemischen mittels Kataphorese
US2878178A (en) 1955-04-15 1959-03-17 Bier Milan Continuous free-boundary flow electrophoresis
US3085956A (en) 1958-12-03 1963-04-16 Nat Res Dev Process and apparatus for effecting electrochromatographic separations
US3149060A (en) 1959-01-13 1964-09-15 Int Minerals & Chem Corp Electrophoresis method and apparatus
US3287244A (en) 1960-03-23 1966-11-22 Howard C Mel Stable flow separation and analytical method
US3125500A (en) 1960-04-05 1964-03-17 Device for the execution of the carrier
NL275701A (de) 1961-03-13
US3320149A (en) 1962-04-16 1967-05-16 Technicon Instr Electrophoresis apparatus
US3140714A (en) 1962-06-28 1964-07-14 Cordis Corp Blood separation method
DE1442414B2 (de) 1963-11-18 1971-02-11 Graßmann, Wolfgang, Prof. Dr., 8000 München Vorrichtung zur Durchfuhrung der trägerfreien, kontinuierlichen Elektrophore se in vertikaler Arbeitsweise
US3498905A (en) 1965-06-18 1970-03-03 Beckman Instruments Inc Continuous flow electrophoresis apparatus
US3412008A (en) 1965-08-23 1968-11-19 Beckman Instruments Inc Electrophoresis apparatus
US3412007A (en) 1965-08-23 1968-11-19 Beckman Instruments Inc Continuous flow electrophoresis apparatus
US3458427A (en) 1965-12-08 1969-07-29 Beckman Instruments Inc Continuous flow electrophoresis apparatus
US3458428A (en) 1966-11-03 1969-07-29 Beckman Instruments Inc Continuous particle electrophoresis apparatus having improved particle band stability
US3509035A (en) 1967-04-14 1970-04-28 Beckman Instruments Inc Continuous particle electrophoresis cell
FR1574986A (de) 1968-05-10 1969-07-18
DE1913411C3 (de) 1969-03-17 1978-04-13 Hoechst Ag, 6000 Frankfurt MembcanzeUe zur Elektrophorese von Substanzgemischen
US3663395A (en) 1969-12-17 1972-05-16 Beckman Instruments Inc Cross-section illuminator for a continuous particle electrophoresis cell
US3655541A (en) 1970-01-22 1972-04-11 Beckman Instruments Inc CONTINUOUS ELECTROPHORESIS CELL WITH LATERAL pH GRADIENT
US3755132A (en) 1971-11-08 1973-08-28 Univ California Fluid belt electrophoresis apparatus
US3758395A (en) 1972-05-15 1973-09-11 Beckman Instruments Inc Is resolution and symmetry control in continuous free flow electrophores
US3821102A (en) 1972-05-31 1974-06-28 Nasa Apparatus for conducting flow electrophoresis in the substantial absence of gravity
US4043895A (en) 1973-05-16 1977-08-23 The Dow Chemical Company Electrophoresis apparatus
US3989613A (en) 1973-05-16 1976-11-02 The Dow Chemical Company Continuous balanced flow fixed boundary electrophoresis
US3847773A (en) 1973-06-11 1974-11-12 Technicon Instr Method and apparatus for curtain electrophoresis
DE2508844C2 (de) 1975-02-28 1984-06-14 Max Planck Gesellschaft zur Förderung der Wissenschaften e.V., 3400 Göttingen Einrichtung zur trägerfreien kontinuierlichen Ablenkungselektrophorese
DE2612005A1 (de) 1976-03-20 1977-09-29 Desaga Gmbh Nachf Erich Fecht Vorrichtung fuer die kontinuierliche elektrophorese im traegerfreien pufferstrom
GB1599671A (en) 1977-08-17 1981-10-07 Atomic Energy Authority Uk Separation process
US4204929A (en) 1978-04-18 1980-05-27 University Patents, Inc. Isoelectric focusing method
US4362612A (en) 1978-04-18 1982-12-07 University Patents, Inc. Isoelectric focusing apparatus
US4214981A (en) 1978-10-23 1980-07-29 University Of Utah Steric field-flow fractionation
US4310408A (en) 1980-02-20 1982-01-12 Mcdonnell Douglas Corporation Electrophoresis chamber
US4383905A (en) 1980-08-15 1983-05-17 Mcdonnell Douglas Corporation System for hydrodynamic compensation for electrophoresis crescent distortion
US4358358A (en) 1981-10-06 1982-11-09 The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration Static continuous electrophoresis device
JPS5897767A (ja) 1981-12-07 1983-06-10 Fuji Xerox Co Ltd 画像処理用磁気デイスク制御方法および装置
US4440638A (en) 1982-02-16 1984-04-03 U.T. Board Of Regents Surface field-effect device for manipulation of charged species
US4394246A (en) 1982-05-24 1983-07-19 Mcdonnell Douglas Corporation Electrophoresis apparatus with flow control
US4465582A (en) 1982-05-24 1984-08-14 Mcdonnell Douglas Corporation Continuous flow electrophoresis apparatus
DE3580418D1 (de) 1984-07-31 1990-12-13 Hitachi Ltd Elektrophoretisches trennverfahren mit freier stroemung und apparat dafuer.
JPS6195241A (ja) 1984-10-17 1986-05-14 Hitachi Ltd 分取用無担体電気泳動法
JPS61162741A (ja) 1985-01-14 1986-07-23 Hitachi Ltd 無担体電気泳動装置の制御法
JPS61215953A (ja) 1985-03-22 1986-09-25 Hitachi Ltd 無担体連続電気泳動装置
JPS61215952A (ja) 1985-03-22 1986-09-25 Hitachi Ltd 無担体電気泳動方法
US5071536A (en) 1985-11-25 1991-12-10 Ivory Cornelius F High resolution continuous flow electrophoresis
US4874507A (en) 1986-06-06 1989-10-17 Whitlock David R Separating constituents of a mixture of particles
US4897169A (en) 1986-08-18 1990-01-30 Milan Bier Process and apparatus for recycling isoelectric focusing and isotachophoresis
JPS6367557A (ja) 1986-09-10 1988-03-26 Hitachi Ltd 無担体連続電気泳動装置
JPS63117252A (ja) 1986-11-05 1988-05-21 Hitachi Ltd 無担体電気泳動方法
GB2225339A (en) 1988-11-15 1990-05-30 Aligena Ag Separating electrically charged macromolecular compounds by forced-flow membrane electrophoresis
US5032247A (en) 1989-09-14 1991-07-16 Separations Technology, Inc. Method and apparatus for electrophoretic separations
WO1991010129A1 (en) 1989-12-13 1991-07-11 Protein Technologies, Inc. A protein purification system based on isoelectric focusing and isotachophoresis
US5133844A (en) 1990-03-15 1992-07-28 United States Department Of Energy Method of electric field flow fractionation wherein the polarity of the electric field is periodically reversed
US5336387A (en) 1990-09-11 1994-08-09 Bioseparations, Inc. Electrical separator apparatus and method of counterflow gradient focusing
US5131994A (en) 1990-12-17 1992-07-21 Shmidt Joseph L Electrophoresis method and apparatus
DE59108006D1 (de) 1991-01-28 1996-08-22 Ciba Geigy Ag Vorrichtung zur Vorbereitung von Proben insbesondere für Analysezwecke
US5122246A (en) 1991-06-26 1992-06-16 Schmidt Joseph L Free flow electrophoresis method
US5439571A (en) 1991-11-19 1995-08-08 Bioseparations, Inc. Apparatus and method for enhanced resolution continuous flow zone electrophoresis
DE4139472C1 (de) * 1991-11-29 1993-03-11 Gerhard Dr. 8011 Kirchheim De Weber
JPH06130035A (ja) 1992-10-15 1994-05-13 Mitsubishi Heavy Ind Ltd 多段型電気泳動装置
US5447612A (en) 1993-02-01 1995-09-05 Protein Technologies, Inc. Buffering system and its use in electrophoretic processes
CN1040117C (zh) * 1993-07-16 1998-10-07 清华大学 色谱式电泳分离方法
US5662813A (en) 1994-10-21 1997-09-02 Bioseparations, Inc. Method for separation of nucleated fetal erythrocytes from maternal blood samples
US5540826A (en) 1995-03-15 1996-07-30 Protein Technologies, Inc. Multi-channel separation device
US5562812A (en) 1995-04-03 1996-10-08 The Penn State Research Foundation Free flow electrophoresis device for biomolecule purification and separation in zero and one G
US5972190A (en) 1996-11-19 1999-10-26 Mcdonnell Douglas Corporation Continuous flow electrophoresis apparatus
DE19711898A1 (de) * 1997-03-21 1998-09-24 Gerhard Dr Weber Verfahren und Vorrichtung zur trägerfreien Ablenkungs-Elektrophorese im Intervall-Betrieb
US6767443B2 (en) 1999-03-29 2004-07-27 Percy H. Rhodes Method and apparatus for electrophoretic focusing
CN2413783Y (zh) * 1999-08-05 2001-01-10 陈福生 一种聚丙烯酰胺凝胶水平电泳槽
JP2001091497A (ja) 1999-09-24 2001-04-06 Technol Res Assoc Of Medical & Welfare Apparatus フリーフロー電気泳動装置
US6758953B2 (en) 1999-10-28 2004-07-06 Nathan A. Thomas Multistage electrophoresis apparatus and method of use for the separation and purification of cells, particles and solutes
JP2001153841A (ja) 1999-11-26 2001-06-08 Olympus Optical Co Ltd 電気泳動装置
US6749733B1 (en) 2000-04-10 2004-06-15 Intel Corporation Materials classifier, method of using, and method of making
AUPQ697300A0 (en) * 2000-04-18 2000-05-11 Life Therapeutics Limited Separation apparatus
JP4103302B2 (ja) 2000-05-15 2008-06-18 株式会社日立製作所 キャピラリアレイを用いた電気泳動装置及びそれに用いられるサンプルプレートアセンブリ
JP3918403B2 (ja) 2000-05-15 2007-05-23 株式会社日立製作所 キャピラリアレイ
DE10047088C2 (de) 2000-09-21 2002-10-17 Gerhard Weber Medium für analytische und präparative Elektrophorese
AUPR110100A0 (en) * 2000-10-30 2000-11-23 Life Therapeutics Limited Improved separation of macromolecules
AU1702902A (en) 2000-11-16 2002-05-27 Basf Ag Method for separating and detecting proteins by means of electrophoresis
DE10063097B4 (de) 2000-12-18 2007-04-19 Becton, Dickinson And Co. Elektrophoresevorrichtung
JP2003028835A (ja) * 2001-07-11 2003-01-29 Olympus Optical Co Ltd フリーフロー電気泳動素子、及びフリーフロー電気泳動法
JP3833110B2 (ja) * 2001-12-05 2006-10-11 オリンパス株式会社 フリーフロー電気泳動法
US7169275B2 (en) 2002-01-21 2007-01-30 Tecan Trading Ag Method for separating particles in free flow electrophoresis
EP1468279A1 (de) * 2002-01-21 2004-10-20 Tecan Trading AG Verfahren sowie vorrichtung zur durchführung des verfahrens und trennmittel zum trennen von teilchen in der free-flow-elektrophorese
US7169278B2 (en) 2002-01-21 2007-01-30 Tecan Trading Ag Apparatus and separation media for separating particles in free-flow electrophoresis
JP3970050B2 (ja) 2002-02-26 2007-09-05 オリンパス株式会社 フリーフロー電気泳動法により可逆的複合体を構成する構成成分間の相互作用を決定する方法および装置
JP2004113079A (ja) 2002-09-25 2004-04-15 Olympus Corp 蛋白質同定方法並びに蛋白質同定装置
US6793791B2 (en) 2003-02-26 2004-09-21 Milan Bier Variable-volume disposable isoelectric focusing cell and method of isoelectric focusing
JP2004286665A (ja) * 2003-03-24 2004-10-14 Olympus Corp フリーフロー電気泳動法

Also Published As

Publication number Publication date
US8721861B2 (en) 2014-05-13
DE102005020134A1 (de) 2006-11-02
US20090218224A1 (en) 2009-09-03
WO2006118996A3 (en) 2007-04-05
DE602006016426D1 (de) 2010-10-07
CN101184991A (zh) 2008-05-21
WO2006118996A2 (en) 2006-11-09
JP2008539443A (ja) 2008-11-13
JP4860693B2 (ja) 2012-01-25
EP1877764A2 (de) 2008-01-16
CN101184991B (zh) 2011-12-21
EP1877764B1 (de) 2010-08-25

Similar Documents

Publication Publication Date Title
ATE479090T1 (de) Elektrophoreseverfahren mit paralleler und gleichzeitiger trennung
WO2009027970A3 (en) Ph gradients controlled by electrolysis, and their use in isoelectric focusing
Malmström et al. Optimized peptide separation and identification for mass spectrometry based proteomics via free-flow electrophoresis
AU2007263005B2 (en) Method and device for separation and depletion of certain proteins and particles using electrophoresis
WO2006091525A3 (en) Electro-blotting devices, systems, and kits, and methods for their use
US6660150B2 (en) Separation of micromolecules
BR122019022434B8 (pt) método para cultivar células de mamífero que secretam proteína heteróloga em um sobrenadante de cultura celular
WO2009146143A3 (en) Ex-vivo multi-dimensional system for the separation and isolation of cells, vesicles, nanoparticles and biomarkers
TW200741005A (en) A purification method of cytidine diphosphate
GB2472943A (en) System and method for increasing polymer/nanopore interactions
ATE425359T1 (de) Elektrokinetische mikropumpe
US20020043465A1 (en) Electrophoresis apparatus and method
AR046180A1 (es) Metodo para producir proteinas gamma carboxiladas y uso terapeutico de las mismas
García et al. Coupling capillary electrophoresis and pulsed electrochemical detection
DK1844143T3 (da) Fremgangsmåde til generering af translationelt aktive lineære DNA-molekyler og anvendelse deraf i array-format
CN109270153A (zh) 一种无两性电解质自由流等电聚焦电泳分离方法
US20070205106A1 (en) Electrophoresis Apparatus
DE602004014758D1 (de) Optimierte Nukleotidsequenzen die für sgp130 kodieren
CN110639365B (zh) 一种用于混合蛋白分离的具有支持介质的制备型垂直流电泳系统
Duša et al. Novel chip-based isoelectric focusing device for fractionation of bacteria prior to their mass spectrometry identification
US10067089B2 (en) Free-flow electrophoresis method for separating analytes
Zhang et al. Mathematical model and dynamic computer simulation on free flow zone electrophoresis
CN201622263U (zh) 垂直电泳系统
Lim Novel devices for analytical-scale isoelectric trapping separations
US20200240951A1 (en) Microchip for free flow electrophoresis

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties