ATE464874T1 - Verfahren zur verwendung von fet-etikettierten oligonukleotiden mit 3',5' exonukleasenresistenter quenchdomäne und zusammensetzungen zur ausübung davon - Google Patents
Verfahren zur verwendung von fet-etikettierten oligonukleotiden mit 3',5' exonukleasenresistenter quenchdomäne und zusammensetzungen zur ausübung davonInfo
- Publication number
- ATE464874T1 ATE464874T1 AT08153863T AT08153863T ATE464874T1 AT E464874 T1 ATE464874 T1 AT E464874T1 AT 08153863 T AT08153863 T AT 08153863T AT 08153863 T AT08153863 T AT 08153863T AT E464874 T1 ATE464874 T1 AT E464874T1
- Authority
- AT
- Austria
- Prior art keywords
- fet
- compositions
- quenchdomain
- labled
- exercising
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6825—Nucleic acid detection involving sensors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US10/087,229 US6818420B2 (en) | 2002-02-27 | 2002-02-27 | Methods of using FET labeled oligonucleotides that include a 3′-5′ exonuclease resistant quencher domain and compositions for practicing the same |
US10/222,943 US7160997B2 (en) | 2002-02-27 | 2002-08-15 | Methods of using FET labeled oligonucleotides that include a 3′→5′ exonuclease resistant quencher domain and compositions for practicing the same |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE464874T1 true ATE464874T1 (de) | 2010-05-15 |
Family
ID=27753904
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT08153863T ATE464874T1 (de) | 2002-02-27 | 2003-02-25 | Verfahren zur verwendung von fet-etikettierten oligonukleotiden mit 3',5' exonukleasenresistenter quenchdomäne und zusammensetzungen zur ausübung davon |
AT03711238T ATE504284T1 (de) | 2002-02-27 | 2003-02-25 | Verfahren zur verwendung von mit fet markierten oligonucleotiden mit einer 3 -5-exonuclease- resistenten quencher-domäne und zusammensetzungen zur durchführung des verfahrens |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT03711238T ATE504284T1 (de) | 2002-02-27 | 2003-02-25 | Verfahren zur verwendung von mit fet markierten oligonucleotiden mit einer 3 -5-exonuclease- resistenten quencher-domäne und zusammensetzungen zur durchführung des verfahrens |
Country Status (4)
Country | Link |
---|---|
US (7) | US6818420B2 (de) |
EP (1) | EP1952792B2 (de) |
AT (2) | ATE464874T1 (de) |
DE (2) | DE60332292D1 (de) |
Families Citing this family (43)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7019129B1 (en) * | 2000-05-09 | 2006-03-28 | Biosearch Technologies, Inc. | Dark quenchers for donor-acceptor energy transfer |
US6818420B2 (en) * | 2002-02-27 | 2004-11-16 | Biosource International, Inc. | Methods of using FET labeled oligonucleotides that include a 3′-5′ exonuclease resistant quencher domain and compositions for practicing the same |
EP1485056B1 (de) * | 2002-02-27 | 2011-04-06 | Life Technologies Corporation | Verfahren zur verwendung von mit fet markierten oligonucleotiden mit einer 3 -5-exonuclease-resistenten quencher-domäne und zusammensetzungen zur durchführung des verfahrens |
US7365176B2 (en) * | 2002-09-26 | 2008-04-29 | Mayo Foundation For Medical Education And Research | Detection of Epstein-Barr virus |
US20040235032A1 (en) * | 2003-05-19 | 2004-11-25 | Canon Kabushiki Kaisha | PCR amplification method, PCR primer set, PCR amplification product, and method for detection of nucleic acid using the amplification method |
EP1502958A1 (de) * | 2003-08-01 | 2005-02-02 | Roche Diagnostics GmbH | Neues Format zur Detektion von Heissstart- und Echtzeit-Polymerasekettenreaktion |
NZ580169A (en) * | 2003-10-23 | 2011-04-29 | Illumigen Biosciences Inc | Detection of mutations in a gene associated with resistance to viral infection, OAS1 (SEQ ID NO:48) |
CN1910293B (zh) * | 2003-10-23 | 2011-04-13 | 伊洛默格生物科学公司 | 检测与抗病毒感染性有关的基因oas1中的突变的方法 |
US20050250134A1 (en) * | 2004-03-02 | 2005-11-10 | Dawei Sheng | Fluorescent energy transfer labeled nucleic acid substrates and methods of use thereof |
JP4972541B2 (ja) | 2004-04-01 | 2012-07-11 | バイオ−ラッド ラボラトリーズ インコーポレーティッド | 標識されたプローブおよび3’→5’エキソヌクレアーゼ活性を用いた定量的増幅方法 |
CA2570693A1 (en) * | 2004-06-17 | 2006-01-26 | Wyeth | Processes for preparing gonadotropin releasing hormone receptor antagonists |
US7696210B2 (en) * | 2004-06-17 | 2010-04-13 | Wyeth | Gonadotropin releasing hormone receptor antagonists |
US7635598B2 (en) * | 2004-07-08 | 2009-12-22 | Biosearch Technologies, Inc. | Inducible fluorescence assay |
MX2007005765A (es) * | 2004-11-23 | 2007-07-19 | Wyeth Corp | Antagonistas del receptor de la hormona que libera gonadotropina. |
US7582634B2 (en) | 2005-02-18 | 2009-09-01 | Wyeth | 7-substituted imidazo[4,5-c]pyridine antagonists of gonadotropin releasing hormone receptor |
US7534796B2 (en) * | 2005-02-18 | 2009-05-19 | Wyeth | Imidazo[4,5-b]pyridine antagonists of gonadotropin releasing hormone receptor |
US7538113B2 (en) * | 2005-02-18 | 2009-05-26 | Wyeth | 4-substituted imidazo[4,5-c]pyridine antagonists of gonadotropin releasing hormone receptor |
US20060189619A1 (en) * | 2005-02-24 | 2006-08-24 | Wyeth | 3-({4-[2-(4-Tert-butylphenyl)-1h-benzimidazol-4-yl]piperazin-1-yl}methyl)pyrido[2,3-b]]pyrazi ne compounds |
UA95446C2 (ru) * | 2005-05-04 | 2011-08-10 | Іллюміджен Байосайєнсіз, Інк. | Мутаци в генах oas1 |
US7531542B2 (en) * | 2005-05-18 | 2009-05-12 | Wyeth | Benzooxazole and benzothiazole antagonists of gonadotropin releasing hormone receptor |
US7582636B2 (en) * | 2005-05-26 | 2009-09-01 | Wyeth | Piperazinylimidazopyridine and piperazinyltriazolopyridine antagonists of Gonadotropin Releasing Hormone receptor |
WO2007056312A2 (en) * | 2005-11-07 | 2007-05-18 | Clontech Laboratories, Inc. | Universal reporter tag for nucleozyme assays |
US8314215B2 (en) * | 2006-08-04 | 2012-11-20 | The Board Of Trustees Of The Leland Stanford Junior University | Mild chemically cleavable linker system |
SI2757091T1 (en) * | 2008-04-01 | 2018-01-31 | Biosearch Technologies, Inc. | Stabilized nucleic acid black gas-fluorofor probe |
KR20110008312A (ko) | 2008-05-06 | 2011-01-26 | 메타볼릭스 인코포레이티드 | 생분합성 폴리에스테르 블렌드 |
US9534255B2 (en) * | 2008-12-17 | 2017-01-03 | Life Technologies Corporation | Methods, compositions, and kits for detecting allelic variants |
EP3249053A1 (de) | 2009-03-27 | 2017-11-29 | Life Technologies Corporation | Verfahren, zusammensetzungen und kits für den nachweis von allelen varianten |
KR20110050327A (ko) * | 2009-11-07 | 2011-05-13 | 주식회사 씨젠 | Thd 프라이머 타겟 검출 |
WO2011078441A1 (en) * | 2009-12-21 | 2011-06-30 | Seegene, Inc. | Tsg primer target detection |
KR20120042100A (ko) * | 2010-10-22 | 2012-05-03 | 주식회사 씨젠 | 이중표지 고정화 프로브를 이용한 고상에서의 타겟 핵산서열 검출 |
CN109054254B (zh) | 2012-08-17 | 2021-02-02 | Cj第一制糖株式会社 | 用于聚合物共混物的生物基橡胶改性剂 |
US10669417B2 (en) | 2013-05-30 | 2020-06-02 | Cj Cheiljedang Corporation | Recyclate blends |
NL2012570B1 (nl) * | 2014-04-04 | 2016-03-08 | Sparkle Innovations B V | Inrichting en werkwijze voor het druppel-voor-druppel toedienen van een vloeistof. |
US9724464B2 (en) * | 2014-06-30 | 2017-08-08 | Carefusion 303, Inc. | Passive restart Y-site |
CN104569599B (zh) * | 2015-01-09 | 2017-10-31 | 杭州电子科技大学 | 一种电子标签电阻在线检测电路 |
US10814006B2 (en) | 2015-07-14 | 2020-10-27 | University Of Iowa Research Foundation | Fluorescent prodrugs |
US11028431B2 (en) * | 2015-09-22 | 2021-06-08 | Biocartis, Nv | Detection of short homopolymeric repeats |
US10337051B2 (en) | 2016-06-16 | 2019-07-02 | The Regents Of The University Of California | Methods and compositions for detecting a target RNA |
WO2020028729A1 (en) | 2018-08-01 | 2020-02-06 | Mammoth Biosciences, Inc. | Programmable nuclease compositions and methods of use thereof |
WO2020142754A2 (en) | 2019-01-04 | 2020-07-09 | Mammoth Biosciences, Inc. | Programmable nuclease improvements and compositions and methods for nucleic acid amplification and detection |
EP4219700A1 (de) | 2019-03-07 | 2023-08-02 | The Regents of the University of California | Crispr-cas-effektorpolypeptide und verfahren zur verwendung davon |
WO2021240443A1 (en) | 2020-05-27 | 2021-12-02 | King Abdullah University Of Science And Technology | Iscan: an rt-lamp-coupled crispr-cas module for rapid, sensitive detection of sars-cov-2 |
WO2023023673A2 (en) | 2021-08-20 | 2023-02-23 | Kasa Bio, L.L.C. | Compositions and methods for multiplex detection of mirna and other polynucelotides |
Family Cites Families (49)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8A (en) * | 1836-08-10 | T Blanchard | Machine for cutting scores around ships' tackle blocks and dead eyes | |
US4743535A (en) | 1984-11-07 | 1988-05-10 | Miles Inc. | Hybridization assay employing labeled probe and anti-hybrid |
EP0187332B1 (de) | 1985-01-10 | 1989-02-01 | Molecular Diagnostics, Inc. | Photochemisches Verfahren zum Markieren von Nucleinsäuren zum Nachweis in Hybridisationsproben |
US4794073A (en) * | 1985-07-10 | 1988-12-27 | Molecular Diagnostics, Inc. | Detection of nucleic acid hybrids by prolonged chemiluminescence |
US5824796A (en) | 1988-09-28 | 1998-10-20 | Epoch Pharmaceuticals, Inc. | Cross-linking oligonucleotides |
US5763162A (en) * | 1990-03-14 | 1998-06-09 | The Regents Of University Of California | Multichromophore fluorescent DNA intercalation complexes |
US5177196A (en) * | 1990-08-16 | 1993-01-05 | Microprobe Corporation | Oligo (α-arabinofuranosyl nucleotides) and α-arabinofuranosyl precursors thereof |
KR100236506B1 (ko) * | 1990-11-29 | 2000-01-15 | 퍼킨-엘머시터스인스트루먼츠 | 폴리머라제 연쇄 반응 수행 장치 |
US6329346B1 (en) | 1991-05-25 | 2001-12-11 | Roche Diagnostics Gmbh | Oligo-2′-deoxynucleotides and their use as pharmaceutical agents with antiviral activity |
US5419966A (en) | 1991-06-10 | 1995-05-30 | Microprobe Corporation | Solid support for synthesis of 3'-tailed oligonucleotides |
CA2074214C (en) * | 1991-07-23 | 2000-02-22 | Will Bloch | Improvements in the in situ pcr |
DE4140463A1 (de) | 1991-12-09 | 1993-06-17 | Boehringer Mannheim Gmbh | 2'-desoxy-isoguanosin, dessen isostere analoge sowie anwendung derselben |
US5538848A (en) * | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
US5840493A (en) * | 1994-03-30 | 1998-11-24 | Mitokor | Mitochondrial DNA mutations that segregate with late onset diabetes mellitus |
US5801155A (en) * | 1995-04-03 | 1998-09-01 | Epoch Pharmaceuticals, Inc. | Covalently linked oligonucleotide minor grove binder conjugates |
US6312894B1 (en) | 1995-04-03 | 2001-11-06 | Epoch Pharmaceuticals, Inc. | Hybridization and mismatch discrimination using oligonucleotides conjugated to minor groove binders |
JP4040087B2 (ja) * | 1995-05-05 | 2008-01-30 | アプレラ コーポレイション | Pcr増幅とハイブリダイゼーションプロービングアッセイとを組み合わせた方法およびそのための試薬 |
US6964861B1 (en) * | 1998-11-13 | 2005-11-15 | Invitrogen Corporation | Enhanced in vitro recombinational cloning of using ribosomal proteins |
PT937098E (pt) * | 1995-06-07 | 2002-12-31 | Invitrogen Corp | Clonagem recombinatoria in vitro utilizando locais de recombinacao modificados |
US5612473A (en) * | 1996-01-16 | 1997-03-18 | Gull Laboratories | Methods, kits and solutions for preparing sample material for nucleic acid amplification |
US5801146A (en) * | 1996-05-03 | 1998-09-01 | Abbott Laboratories | Compound and method for inhibiting angiogenesis |
US5955590A (en) * | 1996-07-15 | 1999-09-21 | Worcester Foundation For Biomedical Research | Conjugates of minor groove DNA binders with antisense oligonucleotides |
US5866336A (en) * | 1996-07-16 | 1999-02-02 | Oncor, Inc. | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
US6117635A (en) * | 1996-07-16 | 2000-09-12 | Intergen Company | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
US5846729A (en) | 1997-02-27 | 1998-12-08 | Lorne Park Research, Inc. | Assaying nucleotides in solution using a fluorescent intensity quenching effect |
DE19720839B4 (de) | 1997-05-17 | 2007-08-16 | Dirk Dr. Schindelhauer | Baukastenprinzip-Technologie zur Herstellung von langen, exakten DNA-Konstrukten in vitro, insbesondere für die Herstellung von Konstrukten zur Erzeugung von künstlichen Chromosomen (MAC, mammalian artificial chromosome) aus zwei stabil klonierten DNA-Komponenten und der ditelomerische Vektor-Prototyp PTAT |
CN100342008C (zh) * | 1997-10-24 | 2007-10-10 | 茵维特罗根公司 | 利用具重组位点的核酸进行重组克隆 |
JP4206154B2 (ja) | 1997-11-13 | 2009-01-07 | 大日本住友製薬株式会社 | 変異型loxP配列とその応用 |
US5989823A (en) * | 1998-09-18 | 1999-11-23 | Nexstar Pharmaceuticals, Inc. | Homogeneous detection of a target through nucleic acid ligand-ligand beacon interaction |
EP0930370B1 (de) * | 1997-12-15 | 2007-08-08 | CSL Behring GmbH | Markierter Primer, geeignet für die Detektion von Nukleinsäuren |
US6127121A (en) | 1998-04-03 | 2000-10-03 | Epoch Pharmaceuticals, Inc. | Oligonucleotides containing pyrazolo[3,4-D]pyrimidines for hybridization and mismatch discrimination |
US6683173B2 (en) * | 1998-04-03 | 2004-01-27 | Epoch Biosciences, Inc. | Tm leveling methods |
GB9812768D0 (en) * | 1998-06-13 | 1998-08-12 | Zeneca Ltd | Methods |
US6072046A (en) * | 1998-08-26 | 2000-06-06 | Epoch Pharmaceuticals, Inc. | Diaziridinyl-aryl and bis-[di(chloroethyl)amino]-aryl oligonucleotide conjugates and reagents for making the same |
US6432642B1 (en) | 1999-01-15 | 2002-08-13 | Pe Corporation (Ny) | Binary probe and clamp composition and methods for a target hybridization detection |
WO2000052027A1 (en) | 1999-03-02 | 2000-09-08 | Invitrogen Corporation | Compositions and methods for use in recombinational cloning of nucleic acids |
WO2000075378A1 (en) | 1999-06-09 | 2000-12-14 | Biosearch Technologies, Inc. | Fluorescence energy transfer probes with stabilized conformations |
US6660845B1 (en) * | 1999-11-23 | 2003-12-09 | Epoch Biosciences, Inc. | Non-aggregating, non-quenching oligomers comprising nucleotide analogues; methods of synthesis and use thereof |
US20040081959A9 (en) | 1999-12-08 | 2004-04-29 | Epoch Biosciences, Inc. | Fluorescent quenching detection reagents and methods |
US6727356B1 (en) * | 1999-12-08 | 2004-04-27 | Epoch Pharmaceuticals, Inc. | Fluorescent quenching detection reagents and methods |
AU785483B2 (en) * | 1999-12-10 | 2007-09-20 | Invitrogen Corporation | Use of multiple recombination sites with unique specificity in recombinational cloning |
EP1995330A1 (de) | 2000-03-01 | 2008-11-26 | Epoch Biosciences, Inc. | Modifizierte Oligonukleotide zur Erkennung von Fehlanpassungen |
DE60138952D1 (de) * | 2000-03-31 | 2011-04-28 | Millenium Pharmaceuticals Inc | 16836, ein mitglied der humanen phospholipase c familie und seine verwendungen |
US7019129B1 (en) | 2000-05-09 | 2006-03-28 | Biosearch Technologies, Inc. | Dark quenchers for donor-acceptor energy transfer |
EP1275735A1 (de) | 2001-07-11 | 2003-01-15 | Roche Diagnostics GmbH | Zusammensetzung und Methode zur 'Hot start' Nukleinsäureamplifizierung |
PT1421200E (pt) * | 2001-08-23 | 2007-02-28 | Merck & Co Inc | Ensaios de pcr multiplex fluorescente para hpv usando múltiplos fluoróforos |
EP1442142A4 (de) | 2001-10-19 | 2006-11-15 | Proligo Llc | Nukleinsäuresonden und verfahren zum nachweis und zur quantifizierung von nukleinsäureanalyten |
EP1485056B1 (de) | 2002-02-27 | 2011-04-06 | Life Technologies Corporation | Verfahren zur verwendung von mit fet markierten oligonucleotiden mit einer 3 -5-exonuclease-resistenten quencher-domäne und zusammensetzungen zur durchführung des verfahrens |
US6818420B2 (en) | 2002-02-27 | 2004-11-16 | Biosource International, Inc. | Methods of using FET labeled oligonucleotides that include a 3′-5′ exonuclease resistant quencher domain and compositions for practicing the same |
-
2002
- 2002-02-27 US US10/087,229 patent/US6818420B2/en not_active Expired - Lifetime
- 2002-08-15 US US10/222,943 patent/US7160997B2/en not_active Expired - Lifetime
-
2003
- 2003-02-25 AT AT08153863T patent/ATE464874T1/de not_active IP Right Cessation
- 2003-02-25 AT AT03711238T patent/ATE504284T1/de not_active IP Right Cessation
- 2003-02-25 DE DE60332292T patent/DE60332292D1/de not_active Expired - Lifetime
- 2003-02-25 EP EP08153863.9A patent/EP1952792B2/de not_active Expired - Lifetime
- 2003-02-25 DE DE60336639T patent/DE60336639D1/de not_active Expired - Lifetime
-
2008
- 2008-08-25 US US12/197,739 patent/US7754453B2/en not_active Expired - Lifetime
-
2010
- 2010-05-24 US US12/786,194 patent/US8088583B2/en not_active Expired - Fee Related
- 2010-11-12 US US12/945,777 patent/US8822673B2/en not_active Expired - Lifetime
-
2014
- 2014-08-13 US US14/459,083 patent/US9410187B2/en not_active Expired - Lifetime
-
2016
- 2016-07-11 US US15/207,011 patent/US20170029874A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
DE60336639D1 (de) | 2011-05-19 |
US9410187B2 (en) | 2016-08-09 |
US6818420B2 (en) | 2004-11-16 |
US20110020812A1 (en) | 2011-01-27 |
US20090081676A1 (en) | 2009-03-26 |
US8822673B2 (en) | 2014-09-02 |
US20030165920A1 (en) | 2003-09-04 |
EP1952792B2 (de) | 2019-01-16 |
US20030162184A1 (en) | 2003-08-28 |
EP1952792A1 (de) | 2008-08-06 |
US20150072342A1 (en) | 2015-03-12 |
US7754453B2 (en) | 2010-07-13 |
US20120245334A1 (en) | 2012-09-27 |
ATE504284T1 (de) | 2011-04-15 |
US20170029874A1 (en) | 2017-02-02 |
US8088583B2 (en) | 2012-01-03 |
US7160997B2 (en) | 2007-01-09 |
EP1952792B1 (de) | 2010-04-21 |
DE60332292D1 (de) | 2010-06-02 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
ATE464874T1 (de) | Verfahren zur verwendung von fet-etikettierten oligonukleotiden mit 3',5' exonukleasenresistenter quenchdomäne und zusammensetzungen zur ausübung davon | |
ATE557083T1 (de) | Thermostabiler oder thermoactiver dna-polymerase mit abgeschwächter 3'-5' exonukleaseaktivität | |
ATE328115T1 (de) | Multiplex genotypisierung unter verwendung von fluoreszenzmarkierten hybridisierungssonden | |
ATE364718T1 (de) | Verfahren zur vervielfältigung von nukleinsäure | |
WO2005089508A3 (en) | Dna sequence detection by limited primer extension | |
DE69729122D1 (de) | KüNSTLICHE FEHLHYBRIDISIERUNG | |
ATE414096T1 (de) | Verfahren zum nachweis und zur quantifizierung von analyten in komplexen mischungen | |
DE69638318D1 (de) | Nukleinsäureliganden, die an DNA-Polymerasen binden und diese inhibieren | |
BR0308134A (pt) | análise rápida das variações em um genoma | |
WO2003072051A3 (en) | Methods of using fet labeled oligonucleotides that include a 3' 5' exonuclease resistant quencher domain and compositions for practicing the same | |
DE69942444D1 (de) | Bestimmung des genotyps eines amplifikationsproduktes an mehreren allelen stellen | |
Doi et al. | A new method for ABO genotyping using a multiplex single-base primer extension reaction and its application to forensic casework samples | |
DE502004004202D1 (de) | Verfahren zum nachweis von cytosin-methylierungen in dna | |
WO2004010101A3 (en) | Detection of chemical ligation using fluorescence quenching leaving groups | |
DE69616102D1 (de) | Amplikationsprimern und Nukleinsäuresonden zum Nachweis von Coccidioides immites | |
ATE331813T1 (de) | Verfahren zum nachweis von homo- oder heterozygotie eines organismus unter verwendung markerter primer und rflp | |
DE502005010036D1 (de) | Verfahren zur geno- und pathotypisierung von pseudomonas aeruginosa | |
ATE440152T1 (de) | Verfahren zum nachweis von cytosinmethylierungsmustern mit hoher empfindlichkeit | |
DK1620568T3 (da) | Analyse til allelspecifik mutationspåvisning | |
ATE414788T1 (de) | Verfahren und kit zur tierartspezifischen dna- identifikation in einer probe | |
DE602005023586D1 (de) | Primer- und sondenkonstruktion zur effizienten amplifikation und detektion des 3'-nichttranslatierten bereichs von hcv | |
Tews et al. | Application of the C4'-alkylated deoxyribose primer system (CAPS) in allele-specific real-time PCR for increased selectivity in discrimination of single nucleotide sequence variants | |
DE50214183D1 (de) | Verfahren zum nachweis von in einer polymerase-kettenreaktion amplifizierten nukleinsäuremolekülen | |
DE60324819D1 (de) | Verfahren zur amplifikation von nukleinsäure, kit zur amplifikation von nukleinsäure, verfahren zum nachweis von einzelnukleotid-polymorphismus und reagentienkit zum nachweis von einzelnukleotid-polymorphismus | |
ATE315103T1 (de) | Verfahren zur typisierung des ha-1 nebenhistokompatibilitätsantigens |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |