ATE459709T1 - Hitzestabiles ligase enthaltendes dns- amplifikationssystem zur bestimmung von genetischen krankheiten - Google Patents
Hitzestabiles ligase enthaltendes dns- amplifikationssystem zur bestimmung von genetischen krankheitenInfo
- Publication number
- ATE459709T1 ATE459709T1 AT04077667T AT04077667T ATE459709T1 AT E459709 T1 ATE459709 T1 AT E459709T1 AT 04077667 T AT04077667 T AT 04077667T AT 04077667 T AT04077667 T AT 04077667T AT E459709 T1 ATE459709 T1 AT E459709T1
- Authority
- AT
- Austria
- Prior art keywords
- amplification system
- dna amplification
- containing dna
- heat stable
- genetic diseases
- Prior art date
Links
- 102000003960 Ligases Human genes 0.000 title abstract 2
- 108090000364 Ligases Proteins 0.000 title abstract 2
- 230000004544 DNA amplification Effects 0.000 title 1
- 208000026350 Inborn Genetic disease Diseases 0.000 title 1
- 208000016361 genetic disease Diseases 0.000 title 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 3
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 2
- 108020004707 nucleic acids Proteins 0.000 abstract 2
- 102000039446 nucleic acids Human genes 0.000 abstract 2
- 102000012410 DNA Ligases Human genes 0.000 abstract 1
- 108010061982 DNA Ligases Proteins 0.000 abstract 1
- 241000589500 Thermus aquaticus Species 0.000 abstract 1
- 238000010367 cloning Methods 0.000 abstract 1
- 230000037430 deletion Effects 0.000 abstract 1
- 238000012217 deletion Methods 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 238000003780 insertion Methods 0.000 abstract 1
- 230000037431 insertion Effects 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 230000005945 translocation Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/93—Ligases (6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6862—Ligase chain reaction [LCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US51844790A | 1990-05-03 | 1990-05-03 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE459709T1 true ATE459709T1 (de) | 2010-03-15 |
Family
ID=24063976
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT04077667T ATE459709T1 (de) | 1990-05-03 | 1991-04-29 | Hitzestabiles ligase enthaltendes dns- amplifikationssystem zur bestimmung von genetischen krankheiten |
| AT91909119T ATE375392T1 (de) | 1990-05-03 | 1991-04-29 | Ein system der dna amplifikation zur detektion genetischer erkrankungen durch eine thermostabile ligase |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT91909119T ATE375392T1 (de) | 1990-05-03 | 1991-04-29 | Ein system der dna amplifikation zur detektion genetischer erkrankungen durch eine thermostabile ligase |
Country Status (7)
| Country | Link |
|---|---|
| EP (2) | EP1507000B1 (https=) |
| JP (3) | JP3889807B2 (https=) |
| AT (2) | ATE459709T1 (https=) |
| DE (2) | DE69133629D1 (https=) |
| DK (1) | DK0528882T3 (https=) |
| ES (1) | ES2294777T3 (https=) |
| WO (1) | WO1991017239A1 (https=) |
Families Citing this family (39)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE173767T1 (de) * | 1992-04-03 | 1998-12-15 | Perkin Elmer Corp | Proben zusammensetzung und verfahren |
| DE4217134A1 (de) * | 1992-05-23 | 1993-11-25 | Boehringer Mannheim Gmbh | Thermostabile Ligase aus Archaebakterien |
| US5700672A (en) | 1992-07-23 | 1997-12-23 | Stratagene | Purified thermostable pyrococcus furiousus DNA ligase |
| CA2140331C (en) * | 1992-08-03 | 2000-01-18 | John J. Carrino | Detecting and amplifying target nucleic acids using exonucleolytic activity |
| US6180338B1 (en) | 1992-08-04 | 2001-01-30 | Beckman Coulter, Inc. | Method, reagent and kit for the detection and amplification of nucleic acid sequences |
| US6207368B1 (en) | 1992-08-04 | 2001-03-27 | Beckman Coulter, Inc. | Methods and reagents for controlling chain extension and ligation chain reactions |
| US5445933A (en) * | 1992-09-15 | 1995-08-29 | Boehringer Mannheim Corporation | Strand displacement assay and complex useful therefor |
| US5462854A (en) * | 1993-04-19 | 1995-10-31 | Beckman Instruments, Inc. | Inverse linkage oligonucleotides for chemical and enzymatic processes |
| ATE174066T1 (de) * | 1994-04-04 | 1998-12-15 | Ciba Corning Diagnostics Corp | Hybridisation-ligitation-verfahren zum nachweis von spezifischen dns sequenzen |
| CA2202990A1 (en) * | 1994-10-21 | 1996-05-02 | Alan H. Davis | Use of spermidine to relieve inhibition of ligase chain reaction in a clinical test sample |
| WO1996014403A1 (en) * | 1994-11-04 | 1996-05-17 | Genis Hf. | A gene from the bacterium rhodothermus marinus, coding for the amino acid sequence of dna-ligase, produced in escherichia coli or some other suitable host organism |
| US6852487B1 (en) | 1996-02-09 | 2005-02-08 | Cornell Research Foundation, Inc. | Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays |
| EP2332958B1 (en) * | 1996-02-09 | 2016-04-20 | Cornell Research Foundation, Inc. | Detection of nucleic and sequence differences using the ligase detection reaction with addressable arrays |
| EP1736554B1 (en) | 1996-05-29 | 2013-10-09 | Cornell Research Foundation, Inc. | Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions |
| AU776810B2 (en) * | 1996-07-19 | 2004-09-23 | Cornell Research Foundation Inc. | High fidelity detection of nucleic acid differences by ligase detection reaction |
| US6312892B1 (en) * | 1996-07-19 | 2001-11-06 | Cornell Research Foundation, Inc. | High fidelity detection of nucleic acid differences by ligase detection reaction |
| ATE362986T1 (de) * | 1998-10-30 | 2007-06-15 | Cornell Res Foundation Inc | High fidelity thermostabile ligase und ihre verwendung |
| US6949370B1 (en) | 1998-10-30 | 2005-09-27 | Cornell Research Foundation, Inc. | High fidelity thermostable ligase and uses thereof |
| US7014994B1 (en) | 1999-03-19 | 2006-03-21 | Cornell Research Foundation,Inc. | Coupled polymerase chain reaction-restriction-endonuclease digestion-ligase detection reaction process |
| DE50001774D1 (de) | 1999-09-29 | 2003-05-22 | Tecan Trading Ag Maennedorf | Thermocycler sowie Hebeelement für Mikrotiterplatte |
| US6272939B1 (en) | 1999-10-15 | 2001-08-14 | Applera Corporation | System and method for filling a substrate with a liquid sample |
| US7169355B1 (en) * | 2000-02-02 | 2007-01-30 | Applera Corporation | Apparatus and method for ejecting sample well trays |
| EP1303639A2 (en) | 2000-04-14 | 2003-04-23 | Cornell Research Foundation, Inc. | Method of designing addressable array for detection of nucleic acid sequence differences using ligase detection reaction |
| US6719949B1 (en) | 2000-06-29 | 2004-04-13 | Applera Corporation | Apparatus and method for transporting sample well trays |
| US6514750B2 (en) | 2001-07-03 | 2003-02-04 | Pe Corporation (Ny) | PCR sample handling device |
| US6942836B2 (en) | 2001-10-16 | 2005-09-13 | Applera Corporation | System for filling substrate chambers with liquid |
| US7309575B2 (en) * | 2002-01-16 | 2007-12-18 | The University Of North Carolina At Chapel Hill | Protein purification and detection methods |
| US20040258563A1 (en) | 2003-06-23 | 2004-12-23 | Applera Corporation | Caps for sample wells and microcards for biological materials |
| WO2005108618A2 (en) | 2004-04-30 | 2005-11-17 | Applera Corporation | Methods and kits for methylation detection |
| WO2006076017A2 (en) | 2004-04-30 | 2006-07-20 | Applera Corporation | Methods and kits for identifying target nucleotides in mixed populations |
| CN101124337B (zh) | 2004-08-24 | 2010-11-17 | 康乃尔研究基金会有限公司 | 使用内切核酸酶剪切/连接酶重新密封反应和毛细管电泳或微阵列检测核酸差异 |
| JP5203202B2 (ja) | 2005-08-16 | 2013-06-05 | メルロジェン， エルエルシー | メルル遺伝子の識別のための方法 |
| US7285388B1 (en) | 2006-04-07 | 2007-10-23 | Merlogen, Llc | Methods for identification of alport syndrome |
| WO2009087686A2 (en) * | 2008-01-04 | 2009-07-16 | Premas Biotech Pvt.Ltd | Nucleic acid based detection methods |
| WO2009087687A1 (en) * | 2008-01-04 | 2009-07-16 | Premas Biotech Pvt.Ltd | Nucleic acid based detection process and uses thereof |
| DK3204520T3 (da) | 2014-10-08 | 2020-04-20 | Univ Cornell | Fremgangsmåde til identificering og kvantificering af nukleinsyreekspression, splejsningsvariant, translokation, kopiantal eller methyleringsændringer |
| CN118984874A (zh) * | 2022-03-25 | 2024-11-19 | 马里兰大学巴尔的摩县 | 用于产生具有单链粘端的双链dna片段的异源双链体热稳定性连接组装(htla)和/或环状异源双链体热稳定性连接组装(chtla) |
| WO2025197400A1 (ja) * | 2024-03-19 | 2025-09-25 | デンカ株式会社 | 高い正確性で、ミスマッチ塩基対を有さない配列を接合するリガーゼ |
| JP2026007482A (ja) * | 2024-07-03 | 2026-01-16 | デンカ株式会社 | キメラリガーゼ |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4883750A (en) * | 1984-12-13 | 1989-11-28 | Applied Biosystems, Inc. | Detection of specific sequences in nucleic acids |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4800159A (en) | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
| US4889818A (en) * | 1986-08-22 | 1989-12-26 | Cetus Corporation | Purified thermostable enzyme |
| WO1989009835A1 (en) * | 1988-04-08 | 1989-10-19 | The Salk Institute For Biological Studies | Ligase-based amplification method |
| US4879214A (en) * | 1988-11-15 | 1989-11-07 | E. I. Du Pont De Nemours And Company | Differentiation of nucleic acid segments on the basis of nucleotide differences |
| AU627809B2 (en) * | 1988-12-16 | 1992-09-03 | Abbott Laboratories | Isolating thermostable enzymes from engineered mesophiles |
-
1991
- 1991-04-29 DE DE69133629T patent/DE69133629D1/de not_active Expired - Lifetime
- 1991-04-29 WO PCT/US1991/002968 patent/WO1991017239A1/en not_active Ceased
- 1991-04-29 ES ES91909119T patent/ES2294777T3/es not_active Expired - Lifetime
- 1991-04-29 DK DK91909119T patent/DK0528882T3/da active
- 1991-04-29 EP EP04077667A patent/EP1507000B1/en not_active Expired - Lifetime
- 1991-04-29 JP JP50889191A patent/JP3889807B2/ja not_active Expired - Lifetime
- 1991-04-29 DE DE69133582T patent/DE69133582T2/de not_active Expired - Lifetime
- 1991-04-29 AT AT04077667T patent/ATE459709T1/de not_active IP Right Cessation
- 1991-04-29 AT AT91909119T patent/ATE375392T1/de not_active IP Right Cessation
- 1991-04-29 EP EP91909119A patent/EP0528882B1/en not_active Expired - Lifetime
-
2001
- 2001-03-05 JP JP2001060432A patent/JP2001269187A/ja active Pending
-
2003
- 2003-11-28 JP JP2003398813A patent/JP2004089204A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| ATE375392T1 (de) | 2007-10-15 |
| DE69133582D1 (de) | 2007-11-22 |
| JPH05508764A (ja) | 1993-12-09 |
| EP0528882A4 (en) | 1993-09-08 |
| EP0528882A1 (en) | 1993-03-03 |
| WO1991017239A1 (en) | 1991-11-14 |
| JP3889807B2 (ja) | 2007-03-07 |
| JP2004089204A (ja) | 2004-03-25 |
| EP1507000A2 (en) | 2005-02-16 |
| DK0528882T3 (da) | 2008-01-14 |
| JP2001269187A (ja) | 2001-10-02 |
| ES2294777T3 (es) | 2008-04-01 |
| EP1507000B1 (en) | 2010-03-03 |
| EP1507000A3 (en) | 2006-03-29 |
| DE69133629D1 (de) | 2010-04-15 |
| EP0528882B1 (en) | 2007-10-10 |
| DE69133582T2 (de) | 2008-07-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ATE459709T1 (de) | Hitzestabiles ligase enthaltendes dns- amplifikationssystem zur bestimmung von genetischen krankheiten | |
| Shih et al. | Thermal denaturation and template properties of DNA complexes with purified histone fractions | |
| Blackburn | Structure and function of telomeres | |
| Dahlberg et al. | Transcription of DNA from the 70 S RNA of Rous Sarcoma Virus I. Identification of a Specific 4 S RNA Which Serves as Primer | |
| Birnstiel et al. | Localization of the Ribosomal DNA Comple | |
| US5403708A (en) | Methods and compositions for determining the sequence of nucleic acids | |
| Gerbi et al. | Replication initiation point mapping | |
| Reha-Krantz et al. | Genetic and biochemical studies of bacteriophage T4 DNA polymerase 3'--> 5'-exonuclease activity. | |
| DE69133204D1 (en) | Gereinigtes thermostabiles nukleinsäure-polymeraseenzym aus thermotoga maritima | |
| ATE135741T1 (de) | Gereinigtes thermostabiles enzym | |
| DE69131321D1 (de) | Mutationen in der 5'-3'-exonukleaseaktivität thermostabiler dna-polymerasen | |
| DK0624641T3 (da) | Termostabil nukleinsyrepolymerase | |
| ES2173867T3 (es) | Uso de nucleotidos externos a la muestra en clonacion genetica. | |
| ATE238434T1 (de) | Eine in kaskaden verlaufende vervielfältigungsreaktion von nukleinsäuren | |
| EP0188450A4 (en) | METHOD FOR DETECTING PATHOGENS OR GENETIC UNITS UNKNOWN IN VITRO AND DETERMINING. | |
| ATE394504T1 (de) | Verfahren zur dns- oder rns-sequenzierung | |
| ATE106948T1 (de) | Verfahren zur amplifizierung von nukleinsäuren. | |
| NO933136L (no) | Bestemmelse av nukleinsyrer ved polymerasekstensjon av oligonukleotider ved anvendelse av terminatorblandinger | |
| FI904494A0 (fi) | Diagnos- och amplifieringsfoerfaranden foer eliminering av ett primer-maol-opar i primerns 3'-aenda. | |
| EP1831401A4 (en) | METHODS, COMPOSITIONS AND KITS FOR FORMING SELF-COMPLEMENTARY POLYNUCLEOTIDES | |
| Murray et al. | Synthesis of single-stranded plasmid pT181 DNA in vitro: initiation and termination of DNA replication | |
| Spratt | Klenow Fragment− DNA Interaction Required for the Incorporation of Nucleotides Opposite Guanine and O 6-Methylguanine | |
| Palmirotta et al. | Use of a multiplex polymerase chain reaction assay in the sex typing of DNA extracted from archaeological bone | |
| ES2553893T3 (es) | ADN polimerasas mutantes con capacidad mejorada de polimerización activada por pirofosforolisis (PAP) | |
| Nguyen et al. | Comparative analysis of the interactions of Escherichia coli σS and σ70 RNA polymerase holoenzyme with the stationary-phase-specific bolAp1 promoter |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |