JP3889807B2 - 遺伝病を検出するための熱安定リガーゼによるｄｎａ増幅系 - Google Patents

遺伝病を検出するための熱安定リガーゼによるｄｎａ増幅系 Download PDF

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Publication number
JP3889807B2
JP3889807B2 JP50889191A JP50889191A JP3889807B2 JP 3889807 B2 JP3889807 B2 JP 3889807B2 JP 50889191 A JP50889191 A JP 50889191A JP 50889191 A JP50889191 A JP 50889191A JP 3889807 B2 JP3889807 B2 JP 3889807B2
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oligonucleotides
nucleotide sequence
oligonucleotide
dna
ligase
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JP50889191A
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Japanese (ja)
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JPH05508764A (ja
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バーラーニイ、フランシス
ジイバラ、ジョン
ニッカーソン、デバラ・エー
カイザー、ロバート・ジェー、ジュニア
フッド、ラロイ
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California Institute of Technology
Cornell Research Foundation Inc
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California Institute of Technology
Cornell Research Foundation Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6862—Ligase chain reaction [LCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP50889191A 1990-05-03 1991-04-29 遺伝病を検出するための熱安定リガーゼによるｄｎａ増幅系 Expired - Lifetime JP3889807B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US51844790A 1990-05-03 1990-05-03
US518,447 1990-05-03
PCT/US1991/002968 WO1991017239A1 (en) 1990-05-03 1991-04-29 A thermostable ligase mediated dna amplification system for the detection of genetic diseases

Related Child Applications (2)

Application Number Title Priority Date Filing Date
JP2001060432A Division JP2001269187A (ja) 1990-05-03 2001-03-05 遺伝病を検出するための熱安定リガーゼによるｄｎａ増幅系
JP2003398813A Division JP2004089204A (ja) 1990-05-03 2003-11-28 遺伝病を検出するための熱安定リガーゼによるｄｎａ増幅系

Publications (2)

Publication Number Publication Date
JPH05508764A JPH05508764A (ja) 1993-12-09
JP3889807B2 true JP3889807B2 (ja) 2007-03-07

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Family Applications (3)

Application Number Title Priority Date Filing Date
JP50889191A Expired - Lifetime JP3889807B2 (ja) 1990-05-03 1991-04-29 遺伝病を検出するための熱安定リガーゼによるｄｎａ増幅系
JP2001060432A Pending JP2001269187A (ja) 1990-05-03 2001-03-05 遺伝病を検出するための熱安定リガーゼによるｄｎａ増幅系
JP2003398813A Pending JP2004089204A (ja) 1990-05-03 2003-11-28 遺伝病を検出するための熱安定リガーゼによるｄｎａ増幅系

Family Applications After (2)

Application Number Title Priority Date Filing Date
JP2001060432A Pending JP2001269187A (ja) 1990-05-03 2001-03-05 遺伝病を検出するための熱安定リガーゼによるｄｎａ増幅系
JP2003398813A Pending JP2004089204A (ja) 1990-05-03 2003-11-28 遺伝病を検出するための熱安定リガーゼによるｄｎａ増幅系

Country Status (7)

Country Link
EP (2) EP1507000B1 (https=)
JP (3) JP3889807B2 (https=)
AT (2) ATE459709T1 (https=)
DE (2) DE69133629D1 (https=)
DK (1) DK0528882T3 (https=)
ES (1) ES2294777T3 (https=)
WO (1) WO1991017239A1 (https=)

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ATE173767T1 (de) * 1992-04-03 1998-12-15 Perkin Elmer Corp Proben zusammensetzung und verfahren
DE4217134A1 (de) * 1992-05-23 1993-11-25 Boehringer Mannheim Gmbh Thermostabile Ligase aus Archaebakterien
US5700672A (en) 1992-07-23 1997-12-23 Stratagene Purified thermostable pyrococcus furiousus DNA ligase
CA2140331C (en) * 1992-08-03 2000-01-18 John J. Carrino Detecting and amplifying target nucleic acids using exonucleolytic activity
US6180338B1 (en) 1992-08-04 2001-01-30 Beckman Coulter, Inc. Method, reagent and kit for the detection and amplification of nucleic acid sequences
US6207368B1 (en) 1992-08-04 2001-03-27 Beckman Coulter, Inc. Methods and reagents for controlling chain extension and ligation chain reactions
US5445933A (en) * 1992-09-15 1995-08-29 Boehringer Mannheim Corporation Strand displacement assay and complex useful therefor
US5462854A (en) * 1993-04-19 1995-10-31 Beckman Instruments, Inc. Inverse linkage oligonucleotides for chemical and enzymatic processes
ATE174066T1 (de) * 1994-04-04 1998-12-15 Ciba Corning Diagnostics Corp Hybridisation-ligitation-verfahren zum nachweis von spezifischen dns sequenzen
CA2202990A1 (en) * 1994-10-21 1996-05-02 Alan H. Davis Use of spermidine to relieve inhibition of ligase chain reaction in a clinical test sample
WO1996014403A1 (en) * 1994-11-04 1996-05-17 Genis Hf. A gene from the bacterium rhodothermus marinus, coding for the amino acid sequence of dna-ligase, produced in escherichia coli or some other suitable host organism
US6852487B1 (en) 1996-02-09 2005-02-08 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
EP2332958B1 (en) * 1996-02-09 2016-04-20 Cornell Research Foundation, Inc. Detection of nucleic and sequence differences using the ligase detection reaction with addressable arrays
EP1736554B1 (en) 1996-05-29 2013-10-09 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
AU776810B2 (en) * 1996-07-19 2004-09-23 Cornell Research Foundation Inc. High fidelity detection of nucleic acid differences by ligase detection reaction
US6312892B1 (en) * 1996-07-19 2001-11-06 Cornell Research Foundation, Inc. High fidelity detection of nucleic acid differences by ligase detection reaction
ATE362986T1 (de) * 1998-10-30 2007-06-15 Cornell Res Foundation Inc High fidelity thermostabile ligase und ihre verwendung
US6949370B1 (en) 1998-10-30 2005-09-27 Cornell Research Foundation, Inc. High fidelity thermostable ligase and uses thereof
US7014994B1 (en) 1999-03-19 2006-03-21 Cornell Research Foundation,Inc. Coupled polymerase chain reaction-restriction-endonuclease digestion-ligase detection reaction process
DE50001774D1 (de) 1999-09-29 2003-05-22 Tecan Trading Ag Maennedorf Thermocycler sowie Hebeelement für Mikrotiterplatte
US6272939B1 (en) 1999-10-15 2001-08-14 Applera Corporation System and method for filling a substrate with a liquid sample
US7169355B1 (en) * 2000-02-02 2007-01-30 Applera Corporation Apparatus and method for ejecting sample well trays
EP1303639A2 (en) 2000-04-14 2003-04-23 Cornell Research Foundation, Inc. Method of designing addressable array for detection of nucleic acid sequence differences using ligase detection reaction
US6719949B1 (en) 2000-06-29 2004-04-13 Applera Corporation Apparatus and method for transporting sample well trays
US6514750B2 (en) 2001-07-03 2003-02-04 Pe Corporation (Ny) PCR sample handling device
US6942836B2 (en) 2001-10-16 2005-09-13 Applera Corporation System for filling substrate chambers with liquid
US7309575B2 (en) * 2002-01-16 2007-12-18 The University Of North Carolina At Chapel Hill Protein purification and detection methods
US20040258563A1 (en) 2003-06-23 2004-12-23 Applera Corporation Caps for sample wells and microcards for biological materials
WO2005108618A2 (en) 2004-04-30 2005-11-17 Applera Corporation Methods and kits for methylation detection
WO2006076017A2 (en) 2004-04-30 2006-07-20 Applera Corporation Methods and kits for identifying target nucleotides in mixed populations
CN101124337B (zh) 2004-08-24 2010-11-17 康乃尔研究基金会有限公司 使用内切核酸酶剪切/连接酶重新密封反应和毛细管电泳或微阵列检测核酸差异
JP5203202B2 (ja) 2005-08-16 2013-06-05 メルロジェン， エルエルシー メルル遺伝子の識別のための方法
US7285388B1 (en) 2006-04-07 2007-10-23 Merlogen, Llc Methods for identification of alport syndrome
WO2009087686A2 (en) * 2008-01-04 2009-07-16 Premas Biotech Pvt.Ltd Nucleic acid based detection methods
WO2009087687A1 (en) * 2008-01-04 2009-07-16 Premas Biotech Pvt.Ltd Nucleic acid based detection process and uses thereof
DK3204520T3 (da) 2014-10-08 2020-04-20 Univ Cornell Fremgangsmåde til identificering og kvantificering af nukleinsyreekspression, splejsningsvariant, translokation, kopiantal eller methyleringsændringer
CN118984874A (zh) * 2022-03-25 2024-11-19 马里兰大学巴尔的摩县 用于产生具有单链粘端的双链dna片段的异源双链体热稳定性连接组装(htla)和/或环状异源双链体热稳定性连接组装(chtla)
WO2025197400A1 (ja) * 2024-03-19 2025-09-25 デンカ株式会社 高い正確性で、ミスマッチ塩基対を有さない配列を接合するリガーゼ
JP2026007482A (ja) * 2024-07-03 2026-01-16 デンカ株式会社 キメラリガーゼ

Family Cites Families (8)

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US4883750A (en) * 1984-12-13 1989-11-28 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US4889818A (en) * 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
WO1989009835A1 (en) * 1988-04-08 1989-10-19 The Salk Institute For Biological Studies Ligase-based amplification method
US4879214A (en) * 1988-11-15 1989-11-07 E. I. Du Pont De Nemours And Company Differentiation of nucleic acid segments on the basis of nucleotide differences
AU627809B2 (en) * 1988-12-16 1992-09-03 Abbott Laboratories Isolating thermostable enzymes from engineered mesophiles

Also Published As

Publication number Publication date
ATE375392T1 (de) 2007-10-15
DE69133582D1 (de) 2007-11-22
JPH05508764A (ja) 1993-12-09
EP0528882A4 (en) 1993-09-08
EP0528882A1 (en) 1993-03-03
WO1991017239A1 (en) 1991-11-14
JP2004089204A (ja) 2004-03-25
EP1507000A2 (en) 2005-02-16
DK0528882T3 (da) 2008-01-14
JP2001269187A (ja) 2001-10-02
ES2294777T3 (es) 2008-04-01
EP1507000B1 (en) 2010-03-03
EP1507000A3 (en) 2006-03-29
DE69133629D1 (de) 2010-04-15
ATE459709T1 (de) 2010-03-15
EP0528882B1 (en) 2007-10-10
DE69133582T2 (de) 2008-07-24

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