ATE420167T1 - Reinigungsverfahren für plasmid-dna - Google Patents
Reinigungsverfahren für plasmid-dnaInfo
- Publication number
- ATE420167T1 ATE420167T1 AT03790033T AT03790033T ATE420167T1 AT E420167 T1 ATE420167 T1 AT E420167T1 AT 03790033 T AT03790033 T AT 03790033T AT 03790033 T AT03790033 T AT 03790033T AT E420167 T1 ATE420167 T1 AT E420167T1
- Authority
- AT
- Austria
- Prior art keywords
- plasmid dna
- purification procedure
- relates
- dna
- dna product
- Prior art date
Links
- 239000013612 plasmid Substances 0.000 title abstract 2
- 238000000746 purification Methods 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Crystallography & Structural Chemistry (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US43527002P | 2002-12-23 | 2002-12-23 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE420167T1 true ATE420167T1 (de) | 2009-01-15 |
Family
ID=32713038
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT03790033T ATE420167T1 (de) | 2002-12-23 | 2003-11-24 | Reinigungsverfahren für plasmid-dna |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US20040157244A1 (enExample) |
| EP (1) | EP1578763B1 (enExample) |
| JP (1) | JP4723254B2 (enExample) |
| AT (1) | ATE420167T1 (enExample) |
| AU (1) | AU2003293042A1 (enExample) |
| CA (1) | CA2509337C (enExample) |
| DE (1) | DE60325773D1 (enExample) |
| WO (1) | WO2004060277A2 (enExample) |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004024067A2 (en) * | 2002-09-10 | 2004-03-25 | Vical Incorporated | Codon-optimized polynucleotide-based vaccines against bacillus anthracis infection |
| US8080642B2 (en) * | 2003-05-16 | 2011-12-20 | Vical Incorporated | Severe acute respiratory syndrome DNA compositions and methods of use |
| PT1664277E (pt) * | 2003-09-17 | 2012-01-17 | Aventis Pharma Sa | Método de preparação de adn plasmídico de grau farmacêutico |
| JP2007532123A (ja) * | 2004-04-19 | 2007-11-15 | センテリオン | 医薬品グレードのプラスミドｄｎａの作成方法 |
| US20060024670A1 (en) | 2004-05-18 | 2006-02-02 | Luke Catherine J | Influenza virus vaccine composition and methods of use |
| JP2006067890A (ja) * | 2004-09-01 | 2006-03-16 | Shigeji Ikeda | 核酸抽出方法および核酸抽出キット |
| KR100624450B1 (ko) * | 2004-12-10 | 2006-09-18 | 삼성전자주식회사 | 히드로겔을 이용한 생물분자의 분리 및 정제 방법 |
| US20070178578A1 (en) * | 2006-01-27 | 2007-08-02 | Nesa & Associates, Inc. | Biofiltration system for treating airborne volatile organic compounds |
| CN100439497C (zh) * | 2006-05-26 | 2008-12-03 | 吉林大学 | 一种适用于大规模生产质粒dna的纯化方法 |
| WO2013067069A2 (en) * | 2011-10-31 | 2013-05-10 | Leinweber Barbara | Dual digestion method for high yield nucleic acid recovery |
| JP2016520687A (ja) * | 2013-04-26 | 2016-07-14 | 日東電工株式会社 | ポリアニオン性ポリマー結合体からのエンドトキシンの減少 |
| EP4127163A1 (en) | 2020-03-31 | 2023-02-08 | Richter-Helm Biologics GmbH & Co. KG | Methods for producing plasmid dna |
| DK3906982T4 (da) * | 2020-05-08 | 2025-11-10 | Axagarius Gmbh & Co Kg | Fremgangsmåde til plasmidrengøring under samtidig reduktion af endotoksiner |
| CN116348598A (zh) * | 2020-10-05 | 2023-06-27 | 赛多利斯比亚分离有限责任公司 | 通过疏水相互作用色谱法改善dna质粒制剂中rna和污染物的去除 |
| US12472148B2 (en) | 2021-04-26 | 2025-11-18 | Celanese Eva Performance Polymers Llc | Implantable device for sustained release of a macromolecular drug compound |
| GB202202777D0 (en) * | 2022-03-01 | 2022-04-13 | Cytiva Bioprocess R & D Ab | A method for separating supercoiled plasmid DNA |
| FR3143127A1 (fr) | 2022-12-07 | 2024-06-14 | Commissariat à l'Energie Atomique et aux Energies Alternatives | Procédé de détection d'une espèce allergène, contaminante ou de nature frauduleuse dans un échantillon agro-alimentaire |
| WO2025157970A1 (en) | 2024-01-26 | 2025-07-31 | Richter Biologics Gmbh & Co. Kg | Large scale methods for producing plasmid dna |
| WO2025252480A1 (en) | 2024-06-07 | 2025-12-11 | F. Hoffmann-La Roche Ag | Method for purifying plasmid dna |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4900677A (en) * | 1986-09-26 | 1990-02-13 | E. I. Du Pont De Nemours And Company | Process for rapid isolation of high molecular weight DNA |
| US4935342A (en) * | 1986-12-01 | 1990-06-19 | Syngene, Inc. | Method of isolating and purifying nucleic acids from biological samples |
| CA1297431C (en) * | 1987-04-24 | 1992-03-17 | F. Hoffmann-La Roche Ag | Process for the isolation of nucleic acids |
| US4923978A (en) * | 1987-12-28 | 1990-05-08 | E. I. Du Pont De Nemours & Company | Process for purifying nucleic acids |
| US5445732A (en) * | 1992-06-19 | 1995-08-29 | Sepracor Inc. | Passivated porous polymer supports and methods for the preparation and use of same |
| US6087491A (en) * | 1993-01-08 | 2000-07-11 | Hybridon, Inc. | Extremely high purity oligonucleotides and methods of synthesizing them using dimer blocks |
| US5561064A (en) * | 1994-02-01 | 1996-10-01 | Vical Incorporated | Production of pharmaceutical-grade plasmid DNA |
| ES2227557T3 (es) * | 1994-07-15 | 2005-04-01 | MERCK & CO., INC. | Procedimiento para la purificacion a gran escala de plasmidos. |
| US5576196A (en) * | 1995-01-13 | 1996-11-19 | Vical Incorporated | Process for reducing RNA concentration in a mixture of biological material using diatomaceous earth |
| US7026468B2 (en) * | 1996-07-19 | 2006-04-11 | Valentis, Inc. | Process and equipment for plasmid purification |
| US6011148A (en) * | 1996-08-01 | 2000-01-04 | Megabios Corporation | Methods for purifying nucleic acids |
| US7807822B2 (en) * | 1996-08-01 | 2010-10-05 | Robert Bridenbaugh | Methods for purifying nucleic acids |
| US5707812A (en) * | 1996-08-06 | 1998-01-13 | Vical Incorporated | Purification of plasmid DNA during column chromatography |
| US6214586B1 (en) * | 1997-12-08 | 2001-04-10 | Genzyme Corporation | Method for purifying plasmid DNA and plasmid DNA substantially free of genomic DNA |
| US6441160B2 (en) * | 1998-05-11 | 2002-08-27 | Tosoh Corporation | Separating plasmids from contaminants using hydrophobic or hydrophobic and ion exchange chromatography |
| GB9907553D0 (en) * | 1999-04-01 | 1999-05-26 | Cantab Pharma Res | Purification of biological preparations |
| US20010044136A1 (en) * | 1999-12-22 | 2001-11-22 | Lander Russel Jackson | Process for the scaleable purification of plasmid DNA |
| GB0107634D0 (en) * | 2001-03-27 | 2001-05-16 | Fermentas Ab | Nucleic acid purification |
-
2003
- 2003-11-24 EP EP03790033A patent/EP1578763B1/en not_active Expired - Lifetime
- 2003-11-24 AT AT03790033T patent/ATE420167T1/de not_active IP Right Cessation
- 2003-11-24 CA CA2509337A patent/CA2509337C/en not_active Expired - Fee Related
- 2003-11-24 DE DE60325773T patent/DE60325773D1/de not_active Expired - Fee Related
- 2003-11-24 AU AU2003293042A patent/AU2003293042A1/en not_active Abandoned
- 2003-11-24 JP JP2004565105A patent/JP4723254B2/ja not_active Expired - Fee Related
- 2003-11-24 US US10/719,978 patent/US20040157244A1/en not_active Abandoned
- 2003-11-24 WO PCT/US2003/037665 patent/WO2004060277A2/en not_active Ceased
-
2010
- 2010-05-04 US US12/773,780 patent/US20100273254A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| EP1578763A2 (en) | 2005-09-28 |
| US20040157244A1 (en) | 2004-08-12 |
| CA2509337C (en) | 2013-01-22 |
| US20100273254A1 (en) | 2010-10-28 |
| JP4723254B2 (ja) | 2011-07-13 |
| WO2004060277A3 (en) | 2005-02-17 |
| WO2004060277A2 (en) | 2004-07-22 |
| EP1578763B1 (en) | 2009-01-07 |
| AU2003293042A8 (en) | 2004-07-29 |
| EP1578763A4 (en) | 2006-12-06 |
| DE60325773D1 (de) | 2009-02-26 |
| JP2006511228A (ja) | 2006-04-06 |
| AU2003293042A1 (en) | 2004-07-29 |
| CA2509337A1 (en) | 2004-07-22 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |