ATE277175T1 - Ein verfahren für die plasmidreinigung in grossem massstab - Google Patents

Ein verfahren für die plasmidreinigung in grossem massstab

Info

Publication number
ATE277175T1
ATE277175T1 AT95927143T AT95927143T ATE277175T1 AT E277175 T1 ATE277175 T1 AT E277175T1 AT 95927143 T AT95927143 T AT 95927143T AT 95927143 T AT95927143 T AT 95927143T AT E277175 T1 ATE277175 T1 AT E277175T1
Authority
AT
Austria
Prior art keywords
large scale
cleaning
disclosed
plasmid dna
plasmids
Prior art date
Application number
AT95927143T
Other languages
English (en)
Inventor
Ann L Lee
Sangeetha Sagar
Original Assignee
Merck & Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck & Co Inc filed Critical Merck & Co Inc
Application granted granted Critical
Publication of ATE277175T1 publication Critical patent/ATE277175T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Detergent Compositions (AREA)
AT95927143T 1994-07-15 1995-07-11 Ein verfahren für die plasmidreinigung in grossem massstab ATE277175T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US27557194A 1994-07-15 1994-07-15
PCT/US1995/008749 WO1996002658A1 (en) 1994-07-15 1995-07-11 A method for large scale plasmid purification

Publications (1)

Publication Number Publication Date
ATE277175T1 true ATE277175T1 (de) 2004-10-15

Family

ID=23052893

Family Applications (1)

Application Number Title Priority Date Filing Date
AT95927143T ATE277175T1 (de) 1994-07-15 1995-07-11 Ein verfahren für die plasmidreinigung in grossem massstab

Country Status (8)

Country Link
EP (1) EP0771355B1 (de)
JP (1) JPH10503086A (de)
AT (1) ATE277175T1 (de)
AU (1) AU708798B2 (de)
CA (1) CA2192342A1 (de)
DE (1) DE69533552T2 (de)
ES (1) ES2227557T3 (de)
WO (1) WO1996002658A1 (de)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AR003122A1 (es) * 1995-05-19 1998-07-08 Merck & Co Inc Un proceso para aislacion y purificacion de plasmidos en fermentadores de gran escala y el adn aislado y purificado obtenido mediante dicho proceso.
KR100318808B1 (ko) 1996-02-06 2004-05-20 로셰 디아그노스틱스 게엠베하 정제핵산의제조방법및그의용도
US5981735A (en) * 1996-02-12 1999-11-09 Cobra Therapeutics Limited Method of plasmid DNA production and purification
GB9602825D0 (en) * 1996-02-12 1996-04-10 Therexsys Ltd Method of plasmid dna production and purification
US7026468B2 (en) 1996-07-19 2006-04-11 Valentis, Inc. Process and equipment for plasmid purification
US7807822B2 (en) * 1996-08-01 2010-10-05 Robert Bridenbaugh Methods for purifying nucleic acids
EP0853123A1 (de) * 1997-01-10 1998-07-15 Roche Diagnostics GmbH Reinigung von DNA durch Cross-Flow-Filtration
FR2773818B1 (fr) * 1998-01-21 2000-02-18 Pasteur Merieux Serums Vacc Procede et dispositif de lyse de bacteries
EP0964057B1 (de) 1998-05-11 2004-12-22 Tosoh Corporation Methode zur Trennung von Nucleinsäuren mittels Flüssigchromatographie
US6268492B1 (en) * 1998-08-10 2001-07-31 Chiron Corporation Methods for purifying non-chromosomal nucleic acid molecules from cells
US6664049B1 (en) 1999-01-20 2003-12-16 Aventis Pasteur S.A. Method and device for cell lysis
CA2378944A1 (en) 1999-07-23 2001-02-01 Genentech, Inc. Method for rnase- and organic solvent-free plasmid dna purification using tangential flow filtration
US20020012990A1 (en) 1999-12-22 2002-01-31 Lander Russel Jackson Process for the scaleable purification of plasmid DNA
US6406892B1 (en) 2001-05-23 2002-06-18 Bio-Rad Laboratories, Inc. Acetate-free purification of plasmid DNA on hydroxyapatite
EP1321176A1 (de) * 2001-12-18 2003-06-25 Boehringer Ingelheim International GmbH Verfahren und Vorrichtung zur Isolierung und Reinigung eines bestimmten Polynukleotides in industriellem Umfang
JP4723254B2 (ja) * 2002-12-23 2011-07-13 バイカル インコーポレイテッド プラスミドｄｎａを精製するための方法
US8501402B2 (en) 2003-03-24 2013-08-06 Boehringer Ingelheim Rcv Gmbh & Co Kg Methods and devices for producing biomolecules
SI1737945T1 (sl) 2004-04-19 2011-05-31 Aventis Pharma Sa Postopek za čiščenje plazmidne DNA
ES2424847T3 (es) 2004-06-25 2013-10-09 Merial Ltd. Genes del virus de la fiebre aftosa que expresan avipox recombinantes
JP4971997B2 (ja) 2005-01-31 2012-07-11 メルク・シャープ・エンド・ドーム・コーポレイション プラスミドｄｎａの精製方法
EP2088196A1 (de) 2008-02-08 2009-08-12 Boehringer Ingelheim RCV GmbH & Co KG Verfahren und Vorrichtungen zur Herstellung von Biomolekülen
CN120882735A (zh) 2023-01-25 2025-10-31 诺韦尔生物技术美国公司 用于产品制造的表达系统

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3928642A (en) * 1975-01-22 1975-12-23 Lilly Co Eli Process for lysing mycelial waste
US4830969A (en) * 1981-08-31 1989-05-16 The Research Foundation Of State University Of New York Process for the rapid and simple isolation of nucleic acids
US5256549A (en) * 1986-03-28 1993-10-26 Chiron Corporation Purification of synthetic oligomers
CA2044593C (en) * 1989-11-03 2004-04-20 Kenneth L. Brigham Method of in vivo delivery of functioning foreign genes
EP0646178A1 (de) * 1992-06-04 1995-04-05 The Regents Of The University Of California Expression kassette mit im säugetier wirt funktionnellen regulator sequenzen
US5834441A (en) * 1993-09-13 1998-11-10 Rhone-Poulenc Rorer Pharmaceuticals Inc. Adeno-associated viral (AAV) liposomes and methods related thereto
AR003122A1 (es) * 1995-05-19 1998-07-08 Merck & Co Inc Un proceso para aislacion y purificacion de plasmidos en fermentadores de gran escala y el adn aislado y purificado obtenido mediante dicho proceso.

Also Published As

Publication number Publication date
AU3126295A (en) 1996-02-16
JPH10503086A (ja) 1998-03-24
AU708798B2 (en) 1999-08-12
EP0771355A1 (de) 1997-05-07
EP0771355B1 (de) 2004-09-22
EP0771355A4 (de) 1997-08-20
ES2227557T3 (es) 2005-04-01
WO1996002658A1 (en) 1996-02-01
DE69533552D1 (de) 2004-10-28
CA2192342A1 (en) 1996-02-01
DE69533552T2 (de) 2005-09-22

Similar Documents

Publication Publication Date Title
ATE277175T1 (de) Ein verfahren für die plasmidreinigung in grossem massstab
MX9708967A (es) Metodo para la purificacion de plasmidos a gran escala.
DE69720473D1 (de) Verfahren zur reinigung von nukleinsäuren
FI971998L (fi) Menetelmä biologisten tuotteiden tuottamiseksi proteiinittomassa viljelmässä
ATE420192T1 (de) Verfahren zur verringerung der antigenität des adeno-assoziierten virus strukturproteins
ITFI930167A0 (it) Impianto a fotobioreattori tubolari per la coltura industriale di microrganismi fotosintetici.
MX9700257A (es) Nuevas estreptograminas y procedimiento de preparacion de estreptograminas por mutasintesis.
AU1322692A (en) Method for the in vitro production of protein from a dna sequence without cloning
DE69523605D1 (de) Verfahren zur herstellung vom aktieven protein dimerischen
DE59606567D1 (de) Neuer Mikroorganismus, dessen Verwendung und Verfahren zur Herstellung von L-Alpha-Aminosäuren
WO1996039501A3 (en) Oligonucleotides specific for human papillomavirus
DE3673054D1 (de) Kontinuierliches verfahren zur herstellung von ethanol durch bakterielle fermentation.
FI20020288A7 (fi) Menetelmä etsyymikatalysoitujen tuotteiden valmistamiseksi ja samanaikaiseksi erottamiseksi
GEP20074226B (en) Novel selective inhibitors of viral or bacterial neuraminidases
DE69609201D1 (de) 21-[2-oxo-tetrahydrofuran]-thio pregnan derivative, ein verfahren zu ihrer herstellung und pharmazeutische präparate davon
ATE321062T1 (de) Verfahren zur herstellung endotoxinfreier nukleinsäuren
DE3569123D1 (en) Process for the isolation and purification of alpha-interferons
ES8704921A1 (es) Un procedimiento para preparar acidos (6,11-dihidro-11-oxodibenz b,c, oxepinil) pentanoicos
ATE346920T1 (de) Neues immunoregulatorisches protein lst-1
DE3583601D1 (de) Plasmid, verfahren zu dessen herstellung, dieses plasmid enthaltender mikroorganismus und verfahren zur zuechtung des mikroorganismus.
HUT72467A (en) 17alpha and 17beta-substituted estra-1,3,5(10)-triene-3-carboxylic acids, pharmaceutical compositions containing them, intermediates and process for their production
TW327123B (en) Preparation method for 3-Ary-4-hydroxy-dihydrofuranone derivatives and intermediates
AU7134991A (en) Intermediates for use in process to produce 2',3' - dideoxynucleosides
IT1293620B1 (it) Processo per la produzione di esopolisaccaridi batterici mediante fermentazione aerobica su substrato solido

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties