WO2004060277A3 - Process for purification of plasmid dna - Google Patents

Process for purification of plasmid dna Download PDF

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Publication number
WO2004060277A3
WO2004060277A3 PCT/US2003/037665 US0337665W WO2004060277A3 WO 2004060277 A3 WO2004060277 A3 WO 2004060277A3 US 0337665 W US0337665 W US 0337665W WO 2004060277 A3 WO2004060277 A3 WO 2004060277A3
Authority
WO
WIPO (PCT)
Prior art keywords
plasmid dna
purification
relates
dna
dna product
Prior art date
Application number
PCT/US2003/037665
Other languages
French (fr)
Other versions
WO2004060277A2 (en
Inventor
Gregg Budahazi
Blake Goff
Original Assignee
Vical Inc
Gregg Budahazi
Blake Goff
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Vical Inc, Gregg Budahazi, Blake Goff filed Critical Vical Inc
Priority to EP03790033A priority Critical patent/EP1578763B1/en
Priority to CA2509337A priority patent/CA2509337C/en
Priority to JP2004565105A priority patent/JP4723254B2/en
Priority to AU2003293042A priority patent/AU2003293042A1/en
Priority to DE60325773T priority patent/DE60325773D1/en
Publication of WO2004060277A2 publication Critical patent/WO2004060277A2/en
Publication of WO2004060277A3 publication Critical patent/WO2004060277A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

The invention relates to a process for purifying plasmid DNA. The invention also relates to a DNA product made by the process of the invention. The DNA product is suitable for pharmaceutical use.
PCT/US2003/037665 2002-12-23 2003-11-24 Process for purification of plasmid dna WO2004060277A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
EP03790033A EP1578763B1 (en) 2002-12-23 2003-11-24 Process for purification of plasmid dna
CA2509337A CA2509337C (en) 2002-12-23 2003-11-24 Process for purification of plasmid dna
JP2004565105A JP4723254B2 (en) 2002-12-23 2003-11-24 Method for purifying plasmid DNA
AU2003293042A AU2003293042A1 (en) 2002-12-23 2003-11-24 Process for purification of plasmid dna
DE60325773T DE60325773D1 (en) 2002-12-23 2003-11-24 PURIFICATION PROCESS FOR PLASMID DNA

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US43527002P 2002-12-23 2002-12-23
US60/435,270 2002-12-23

Publications (2)

Publication Number Publication Date
WO2004060277A2 WO2004060277A2 (en) 2004-07-22
WO2004060277A3 true WO2004060277A3 (en) 2005-02-17

Family

ID=32713038

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/037665 WO2004060277A2 (en) 2002-12-23 2003-11-24 Process for purification of plasmid dna

Country Status (8)

Country Link
US (2) US20040157244A1 (en)
EP (1) EP1578763B1 (en)
JP (1) JP4723254B2 (en)
AT (1) ATE420167T1 (en)
AU (1) AU2003293042A1 (en)
CA (1) CA2509337C (en)
DE (1) DE60325773D1 (en)
WO (1) WO2004060277A2 (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1575505A4 (en) * 2002-09-10 2007-01-24 Vical Inc Codon-optimized polynucleotide-based vaccines against bacillus anthracis infection
US8080642B2 (en) * 2003-05-16 2011-12-20 Vical Incorporated Severe acute respiratory syndrome DNA compositions and methods of use
BRPI0413907A (en) * 2003-09-17 2006-10-24 Centelion pharmaceutical grade dna plasmid preparation method
WO2005100542A1 (en) * 2004-04-19 2005-10-27 Centelion Method for purifying plasmid dna
US20060024670A1 (en) 2004-05-18 2006-02-02 Luke Catherine J Influenza virus vaccine composition and methods of use
JP2006067890A (en) * 2004-09-01 2006-03-16 Shigeji Ikeda Method for extracting nucleic acid and nucleic acid-extracting kit
KR100624450B1 (en) * 2004-12-10 2006-09-18 삼성전자주식회사 Isolation and purification method of biomolecules using hydrogel
US20070178578A1 (en) * 2006-01-27 2007-08-02 Nesa & Associates, Inc. Biofiltration system for treating airborne volatile organic compounds
CN100439497C (en) * 2006-05-26 2008-12-03 吉林大学 Purification method adapted to mass production of plasmid DNA
WO2013067069A2 (en) * 2011-10-31 2013-05-10 Leinweber Barbara Dual digestion method for high yield nucleic acid recovery
CN105143244A (en) * 2013-04-26 2015-12-09 日东电工株式会社 Reduction of endotoxins from polyanionic polymer conjugates
WO2021198281A1 (en) 2020-03-31 2021-10-07 Richter-Helm Biologics Gmbh & Co. Kg Methods for producing plasmid dna
DK3906982T3 (en) * 2020-05-08 2022-04-25 Axagarius Gmbh & Co Kg Method for plasmid cleaning while simultaneously reducing endotoxins
GB202202777D0 (en) * 2022-03-01 2022-04-13 Cytiva Bioprocess R & D Ab A method for separating supercoiled plasmid DNA
FR3143127A1 (en) 2022-12-07 2024-06-14 Commissariat à l'Energie Atomique et aux Energies Alternatives Method for detecting an allergenic, contaminating or fraudulent species in an agri-food sample

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20010034435A1 (en) * 1996-07-19 2001-10-25 Valentis, Inc. Process and equipment for plasmid purification

Family Cites Families (17)

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Publication number Priority date Publication date Assignee Title
US4900677A (en) * 1986-09-26 1990-02-13 E. I. Du Pont De Nemours And Company Process for rapid isolation of high molecular weight DNA
US4935342A (en) * 1986-12-01 1990-06-19 Syngene, Inc. Method of isolating and purifying nucleic acids from biological samples
CA1297431C (en) * 1987-04-24 1992-03-17 F. Hoffmann-La Roche Ag Process for the isolation of nucleic acids
US4923978A (en) * 1987-12-28 1990-05-08 E. I. Du Pont De Nemours & Company Process for purifying nucleic acids
US5445732A (en) * 1992-06-19 1995-08-29 Sepracor Inc. Passivated porous polymer supports and methods for the preparation and use of same
US6087491A (en) * 1993-01-08 2000-07-11 Hybridon, Inc. Extremely high purity oligonucleotides and methods of synthesizing them using dimer blocks
US5561064A (en) * 1994-02-01 1996-10-01 Vical Incorporated Production of pharmaceutical-grade plasmid DNA
JPH10503086A (en) * 1994-07-15 1998-03-24 メルク エンド カンパニー インコーポレーテッド Large-scale plasmid purification method
US5576196A (en) * 1995-01-13 1996-11-19 Vical Incorporated Process for reducing RNA concentration in a mixture of biological material using diatomaceous earth
US6011148A (en) * 1996-08-01 2000-01-04 Megabios Corporation Methods for purifying nucleic acids
US7807822B2 (en) * 1996-08-01 2010-10-05 Robert Bridenbaugh Methods for purifying nucleic acids
US5707812A (en) * 1996-08-06 1998-01-13 Vical Incorporated Purification of plasmid DNA during column chromatography
US6214586B1 (en) * 1997-12-08 2001-04-10 Genzyme Corporation Method for purifying plasmid DNA and plasmid DNA substantially free of genomic DNA
DE69922740T2 (en) * 1998-05-11 2005-12-08 Tosoh Corp., Shinnanyo Method for the separation of nucleic acids by liquid chromatography
GB9907553D0 (en) * 1999-04-01 1999-05-26 Cantab Pharma Res Purification of biological preparations
ATE371731T1 (en) * 1999-12-22 2007-09-15 Merck & Co Inc METHOD FOR THE SCALABLE PURIFICATION OF PLASMID DNA
GB0107634D0 (en) * 2001-03-27 2001-05-16 Fermentas Ab Nucleic acid purification

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20010034435A1 (en) * 1996-07-19 2001-10-25 Valentis, Inc. Process and equipment for plasmid purification

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DIOGO M.M. ET AL.: "Separation and analysis of plasmid denatured forms using hydrophobic interaction chromatography", ANALYTICAL BIOCHEMISTRY, vol. 275, November 1999 (1999-11-01), pages 122 - 124, XP002178574 *
FERREIRA G.N.M. ET AL.: "Downstream processing of plasmid DNA for gene therapy and DNA vaccine applications", vol. 18, September 2000 (2000-09-01), pages 380 - 388, XP000972180 *

Also Published As

Publication number Publication date
CA2509337C (en) 2013-01-22
WO2004060277A2 (en) 2004-07-22
DE60325773D1 (en) 2009-02-26
CA2509337A1 (en) 2004-07-22
EP1578763A2 (en) 2005-09-28
US20100273254A1 (en) 2010-10-28
EP1578763A4 (en) 2006-12-06
US20040157244A1 (en) 2004-08-12
ATE420167T1 (en) 2009-01-15
JP4723254B2 (en) 2011-07-13
EP1578763B1 (en) 2009-01-07
AU2003293042A1 (en) 2004-07-29
JP2006511228A (en) 2006-04-06
AU2003293042A8 (en) 2004-07-29

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