DE60325773D1 - Reinigungsverfahren für plasmid-dna - Google Patents

Reinigungsverfahren für plasmid-dna

Info

Publication number
DE60325773D1
DE60325773D1 DE60325773T DE60325773T DE60325773D1 DE 60325773 D1 DE60325773 D1 DE 60325773D1 DE 60325773 T DE60325773 T DE 60325773T DE 60325773 T DE60325773 T DE 60325773T DE 60325773 D1 DE60325773 D1 DE 60325773D1
Authority
DE
Germany
Prior art keywords
plasmid dna
purification process
relates
dna
dna product
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE60325773T
Other languages
English (en)
Inventor
Gregg Budahazi
Blake Goff
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fresh Tracks Therapeutics Inc
Original Assignee
Vical Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Vical Inc filed Critical Vical Inc
Application granted granted Critical
Publication of DE60325773D1 publication Critical patent/DE60325773D1/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
DE60325773T 2002-12-23 2003-11-24 Reinigungsverfahren für plasmid-dna Expired - Fee Related DE60325773D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US43527002P 2002-12-23 2002-12-23
PCT/US2003/037665 WO2004060277A2 (en) 2002-12-23 2003-11-24 Process for purification of plasmid dna

Publications (1)

Publication Number Publication Date
DE60325773D1 true DE60325773D1 (de) 2009-02-26

Family

ID=32713038

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60325773T Expired - Fee Related DE60325773D1 (de) 2002-12-23 2003-11-24 Reinigungsverfahren für plasmid-dna

Country Status (8)

Country Link
US (2) US20040157244A1 (de)
EP (1) EP1578763B1 (de)
JP (1) JP4723254B2 (de)
AT (1) ATE420167T1 (de)
AU (1) AU2003293042A1 (de)
CA (1) CA2509337C (de)
DE (1) DE60325773D1 (de)
WO (1) WO2004060277A2 (de)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004024067A2 (en) * 2002-09-10 2004-03-25 Vical Incorporated Codon-optimized polynucleotide-based vaccines against bacillus anthracis infection
US8080642B2 (en) * 2003-05-16 2011-12-20 Vical Incorporated Severe acute respiratory syndrome DNA compositions and methods of use
ES2376173T3 (es) * 2003-09-17 2012-03-09 Aventis Pharma S.A. Método de preparación de adn plasm�?dico de grado farmacéutico.
KR20060135062A (ko) * 2004-04-19 2006-12-28 센텔리옹 에스아에스 플라스미드 ｄｎａ의 정제 방법
CA2566355C (en) * 2004-05-18 2014-04-15 Vical Incorporated Influenza virus vaccine composition and methods of use
JP2006067890A (ja) * 2004-09-01 2006-03-16 Shigeji Ikeda 核酸抽出方法および核酸抽出キット
KR100624450B1 (ko) * 2004-12-10 2006-09-18 삼성전자주식회사 히드로겔을 이용한 생물분자의 분리 및 정제 방법
US20070178578A1 (en) * 2006-01-27 2007-08-02 Nesa & Associates, Inc. Biofiltration system for treating airborne volatile organic compounds
CN100439497C (zh) * 2006-05-26 2008-12-03 吉林大学 一种适用于大规模生产质粒dna的纯化方法
WO2013067069A2 (en) * 2011-10-31 2013-05-10 Leinweber Barbara Dual digestion method for high yield nucleic acid recovery
WO2014175899A1 (en) * 2013-04-26 2014-10-30 Nitto Denko Corporation Reduction of endotoxins from polyanionic polymer conjugates
EP4127163A1 (de) 2020-03-31 2023-02-08 Richter-Helm Biologics GmbH & Co. KG Verfahren zur herstellung von plasmid-dna
DK3906982T3 (da) * 2020-05-08 2022-04-25 Axagarius Gmbh & Co Kg Fremgangsmåde til plasmidrengøring under samtidig reduktion af endotoksiner
GB202202777D0 (en) * 2022-03-01 2022-04-13 Cytiva Bioprocess R & D Ab A method for separating supercoiled plasmid DNA

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4900677A (en) * 1986-09-26 1990-02-13 E. I. Du Pont De Nemours And Company Process for rapid isolation of high molecular weight DNA
US4935342A (en) * 1986-12-01 1990-06-19 Syngene, Inc. Method of isolating and purifying nucleic acids from biological samples
CA1297431C (en) * 1987-04-24 1992-03-17 F. Hoffmann-La Roche Ag Process for the isolation of nucleic acids
US4923978A (en) * 1987-12-28 1990-05-08 E. I. Du Pont De Nemours & Company Process for purifying nucleic acids
US5445732A (en) * 1992-06-19 1995-08-29 Sepracor Inc. Passivated porous polymer supports and methods for the preparation and use of same
US6087491A (en) * 1993-01-08 2000-07-11 Hybridon, Inc. Extremely high purity oligonucleotides and methods of synthesizing them using dimer blocks
US5561064A (en) * 1994-02-01 1996-10-01 Vical Incorporated Production of pharmaceutical-grade plasmid DNA
JPH10503086A (ja) * 1994-07-15 1998-03-24 メルク エンド カンパニー インコーポレーテッド 大規模プラスミド精製方法
US5576196A (en) * 1995-01-13 1996-11-19 Vical Incorporated Process for reducing RNA concentration in a mixture of biological material using diatomaceous earth
US7026468B2 (en) * 1996-07-19 2006-04-11 Valentis, Inc. Process and equipment for plasmid purification
US6011148A (en) * 1996-08-01 2000-01-04 Megabios Corporation Methods for purifying nucleic acids
US7807822B2 (en) * 1996-08-01 2010-10-05 Robert Bridenbaugh Methods for purifying nucleic acids
US5707812A (en) * 1996-08-06 1998-01-13 Vical Incorporated Purification of plasmid DNA during column chromatography
US6214586B1 (en) * 1997-12-08 2001-04-10 Genzyme Corporation Method for purifying plasmid DNA and plasmid DNA substantially free of genomic DNA
US6441160B2 (en) * 1998-05-11 2002-08-27 Tosoh Corporation Separating plasmids from contaminants using hydrophobic or hydrophobic and ion exchange chromatography
GB9907553D0 (en) * 1999-04-01 1999-05-26 Cantab Pharma Res Purification of biological preparations
JP4856833B2 (ja) * 1999-12-22 2012-01-18 メルク・シャープ・エンド・ドーム・コーポレイション プラスミドｄｎａの大規模化可能な精製方法
GB0107634D0 (en) * 2001-03-27 2001-05-16 Fermentas Ab Nucleic acid purification

Also Published As

Publication number Publication date
AU2003293042A1 (en) 2004-07-29
EP1578763B1 (de) 2009-01-07
WO2004060277A3 (en) 2005-02-17
ATE420167T1 (de) 2009-01-15
EP1578763A2 (de) 2005-09-28
CA2509337A1 (en) 2004-07-22
AU2003293042A8 (en) 2004-07-29
US20040157244A1 (en) 2004-08-12
CA2509337C (en) 2013-01-22
JP4723254B2 (ja) 2011-07-13
JP2006511228A (ja) 2006-04-06
US20100273254A1 (en) 2010-10-28
WO2004060277A2 (en) 2004-07-22
EP1578763A4 (de) 2006-12-06

Similar Documents

Publication Publication Date Title
DE60325773D1 (de) Reinigungsverfahren für plasmid-dna
BRPI0410685A (pt) processos de purificação de 1,3-propanodiol e composições
SI1599478T1 (sl) Dihidropteridinoni, postopek za njihovo pripravo in njihova uporaba kot zdravila
BR0307432B1 (pt) difluormetiltiazolilcarboxanilidas
DE602004016796D1 (de) Reinigungverfahren für Synthesegas
WO2002098865A3 (fr) Inhibiteurs des phosphodiesterases des nucleotides cycliques, preparation et utilisations de ces inhibiteurs
YU71102A (sh) Supstituisani amidi benzoeve kiseline i njihova primena kao sredstva za lečenje
FR2832141B1 (fr) Procede de purification de gaz de synthese
DE602004006157D1 (de) Verfahren zur aufreinigung von il-18-bindendem protein
TNSN05294A1 (en) Immunosuppressant compounds and compositions
MXPA05012461A (es) Compuestos y composiciones inmunosupresores.
EP1638551A4 (de) Immunosuppressive verbindungen und zusammensetzungen
CY1109055T1 (el) 11-((4r,6r)-4,5,6-τριϋδροξυ-3-(r)-υδροξυ-εξυλοκαρβαμοϋλο)-ενδεκαϊνικο οξυ
BRPI0606170A2 (pt) purificação diastereomérica de rosuvastatina
CY1107777T1 (el) Μεθοδος για τον κλιμακουμενο καθαρισμο πλασμιδιακου dna
BR0011033A (pt) Métodos de purificação de dna e dna purificado
BRPI0511146A (pt) processos de preparação e purificação de dialquiléster de ácido naftalenodicarboxìlico
DE602005020185D1 (de) Verfahren zur herstellung von 17-hydroxy-6 beta, 7 beta, 15 beta,16 beta-bismethylen-17alpha-pregn-4henprodukte für dieses verfahren
BRPI0605902A (pt) processo de purificação para intermediário de anastrozol
DE502004001922D1 (de) Verfahren zur herstellung von ethylenaminen
YU63696A (sh) Novi fenilamidin-derivati, postupci za njihovo pripremanje i njihova primena kao lekova
EP1728787A4 (de) Verfahren zur aufreinigung von 2-chlor-5-chlormethyl-1,3-thiazol
ATE252592T1 (de) Verfahren zur reinigung von 2-keto-l-gulonic säure
DE60233064D1 (de) Analgetisches antitumorpeptid aus skorpionen und verfahren zu dessen herstellung
ZA200600598B (en) Method for production of $g(alpha),$g(beta)-unsaturated amide compounds

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee