AU2003293042A1 - Process for purification of plasmid dna - Google Patents

Process for purification of plasmid dna

Info

Publication number
AU2003293042A1
AU2003293042A1 AU2003293042A AU2003293042A AU2003293042A1 AU 2003293042 A1 AU2003293042 A1 AU 2003293042A1 AU 2003293042 A AU2003293042 A AU 2003293042A AU 2003293042 A AU2003293042 A AU 2003293042A AU 2003293042 A1 AU2003293042 A1 AU 2003293042A1
Authority
AU
Australia
Prior art keywords
plasmid dna
purification
relates
dna
dna product
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2003293042A
Other languages
English (en)
Other versions
AU2003293042A8 (en
Inventor
Gregg Budahazi
Blake Goff
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fresh Tracks Therapeutics Inc
Original Assignee
Vical Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Vical Inc filed Critical Vical Inc
Publication of AU2003293042A1 publication Critical patent/AU2003293042A1/en
Publication of AU2003293042A8 publication Critical patent/AU2003293042A8/xx
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
AU2003293042A 2002-12-23 2003-11-24 Process for purification of plasmid dna Abandoned AU2003293042A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US43527002P 2002-12-23 2002-12-23
US60/435,270 2002-12-23
PCT/US2003/037665 WO2004060277A2 (en) 2002-12-23 2003-11-24 Process for purification of plasmid dna

Publications (2)

Publication Number Publication Date
AU2003293042A1 true AU2003293042A1 (en) 2004-07-29
AU2003293042A8 AU2003293042A8 (en) 2004-07-29

Family

ID=32713038

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2003293042A Abandoned AU2003293042A1 (en) 2002-12-23 2003-11-24 Process for purification of plasmid dna

Country Status (8)

Country Link
US (2) US20040157244A1 (enExample)
EP (1) EP1578763B1 (enExample)
JP (1) JP4723254B2 (enExample)
AT (1) ATE420167T1 (enExample)
AU (1) AU2003293042A1 (enExample)
CA (1) CA2509337C (enExample)
DE (1) DE60325773D1 (enExample)
WO (1) WO2004060277A2 (enExample)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004024067A2 (en) * 2002-09-10 2004-03-25 Vical Incorporated Codon-optimized polynucleotide-based vaccines against bacillus anthracis infection
US8080642B2 (en) * 2003-05-16 2011-12-20 Vical Incorporated Severe acute respiratory syndrome DNA compositions and methods of use
EP1664277B1 (en) * 2003-09-17 2011-11-09 Aventis Pharma S.A. Method of preparation of pharmaceutically grade plasmid dna
CA2559368A1 (en) * 2004-04-19 2005-10-27 Centelion Method for purifying plasmid dna
AU2005248361B2 (en) * 2004-05-18 2010-03-11 Vical Incorporated Influenza virus vaccine composition and methods of use
JP2006067890A (ja) * 2004-09-01 2006-03-16 Shigeji Ikeda 核酸抽出方法および核酸抽出キット
KR100624450B1 (ko) * 2004-12-10 2006-09-18 삼성전자주식회사 히드로겔을 이용한 생물분자의 분리 및 정제 방법
US20070178578A1 (en) * 2006-01-27 2007-08-02 Nesa & Associates, Inc. Biofiltration system for treating airborne volatile organic compounds
CN100439497C (zh) * 2006-05-26 2008-12-03 吉林大学 一种适用于大规模生产质粒dna的纯化方法
WO2013067069A2 (en) * 2011-10-31 2013-05-10 Leinweber Barbara Dual digestion method for high yield nucleic acid recovery
EP2989116A4 (en) * 2013-04-26 2017-01-18 Nitto Denko Corporation Reduction of endotoxins from polyanionic polymer conjugates
IL296722A (en) 2020-03-31 2022-11-01 Richter Helm Biologics Gmbh & Co Kg Methods for the production of plasmid DNA
DK3906982T4 (da) * 2020-05-08 2025-11-10 Axagarius Gmbh & Co Kg Fremgangsmåde til plasmidrengøring under samtidig reduktion af endotoksiner
WO2022073995A1 (en) * 2020-10-05 2022-04-14 Bia Separations D.O.O. Improved removal of rna and contaminants from dna plasmid preparations by hydrophobic interaction chromatography
US12472148B2 (en) 2021-04-26 2025-11-18 Celanese Eva Performance Polymers Llc Implantable device for sustained release of a macromolecular drug compound
GB202202777D0 (en) * 2022-03-01 2022-04-13 Cytiva Bioprocess R & D Ab A method for separating supercoiled plasmid DNA
FR3143127A1 (fr) 2022-12-07 2024-06-14 Commissariat à l'Energie Atomique et aux Energies Alternatives Procédé de détection d'une espèce allergène, contaminante ou de nature frauduleuse dans un échantillon agro-alimentaire
WO2025157970A1 (en) 2024-01-26 2025-07-31 Richter Biologics Gmbh & Co. Kg Large scale methods for producing plasmid dna
WO2025252480A1 (en) 2024-06-07 2025-12-11 F. Hoffmann-La Roche Ag Method for purifying plasmid dna

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4900677A (en) * 1986-09-26 1990-02-13 E. I. Du Pont De Nemours And Company Process for rapid isolation of high molecular weight DNA
US4935342A (en) * 1986-12-01 1990-06-19 Syngene, Inc. Method of isolating and purifying nucleic acids from biological samples
CA1297431C (en) * 1987-04-24 1992-03-17 F. Hoffmann-La Roche Ag Process for the isolation of nucleic acids
US4923978A (en) * 1987-12-28 1990-05-08 E. I. Du Pont De Nemours & Company Process for purifying nucleic acids
US5445732A (en) * 1992-06-19 1995-08-29 Sepracor Inc. Passivated porous polymer supports and methods for the preparation and use of same
US6087491A (en) * 1993-01-08 2000-07-11 Hybridon, Inc. Extremely high purity oligonucleotides and methods of synthesizing them using dimer blocks
US5561064A (en) * 1994-02-01 1996-10-01 Vical Incorporated Production of pharmaceutical-grade plasmid DNA
ATE277175T1 (de) * 1994-07-15 2004-10-15 Merck & Co Inc Ein verfahren für die plasmidreinigung in grossem massstab
US5576196A (en) * 1995-01-13 1996-11-19 Vical Incorporated Process for reducing RNA concentration in a mixture of biological material using diatomaceous earth
US7026468B2 (en) * 1996-07-19 2006-04-11 Valentis, Inc. Process and equipment for plasmid purification
US7807822B2 (en) * 1996-08-01 2010-10-05 Robert Bridenbaugh Methods for purifying nucleic acids
US6011148A (en) * 1996-08-01 2000-01-04 Megabios Corporation Methods for purifying nucleic acids
US5707812A (en) * 1996-08-06 1998-01-13 Vical Incorporated Purification of plasmid DNA during column chromatography
US6214586B1 (en) * 1997-12-08 2001-04-10 Genzyme Corporation Method for purifying plasmid DNA and plasmid DNA substantially free of genomic DNA
EP0964057B1 (en) * 1998-05-11 2004-12-22 Tosoh Corporation Method for separating nucleic acids by means of liquid chromatography
GB9907553D0 (en) * 1999-04-01 1999-05-26 Cantab Pharma Res Purification of biological preparations
DE60036226T2 (de) * 1999-12-22 2008-05-21 Merck & Co., Inc. Verfahren zur skalierbaren reinigung von plasmid-dns
GB0107634D0 (en) * 2001-03-27 2001-05-16 Fermentas Ab Nucleic acid purification

Also Published As

Publication number Publication date
ATE420167T1 (de) 2009-01-15
EP1578763B1 (en) 2009-01-07
WO2004060277A2 (en) 2004-07-22
US20040157244A1 (en) 2004-08-12
AU2003293042A8 (en) 2004-07-29
JP4723254B2 (ja) 2011-07-13
EP1578763A4 (en) 2006-12-06
WO2004060277A3 (en) 2005-02-17
CA2509337A1 (en) 2004-07-22
EP1578763A2 (en) 2005-09-28
DE60325773D1 (de) 2009-02-26
CA2509337C (en) 2013-01-22
US20100273254A1 (en) 2010-10-28
JP2006511228A (ja) 2006-04-06

Similar Documents

Publication Publication Date Title
AU2003293042A1 (en) Process for purification of plasmid dna
BRPI0410685A (pt) processos de purificação de 1,3-propanodiol e composições
WO2002098865A3 (fr) Inhibiteurs des phosphodiesterases des nucleotides cycliques, preparation et utilisations de ces inhibiteurs
PL392725A1 (pl) Nowe związki pośrednie do syntezy związków triazolopirymidyny oraz sposób ich wytwarzania
WO2004055006A8 (en) Novel compounds having selective inhibiting effect at gsk3
AU2003202585A1 (en) Disubstituted thiazolyl carboxanilides and their use as microbicides
TW200508201A (en) Novel process for the preparation of roflumilast
AU2003294723A1 (en) Process for the purification of crude propene oxide
WO2004014902A3 (en) Compounds having an activity at metabotropic glutamate receptors
PL371582A1 (en) Difluoromethyl thiazolyl carboxanilides
WO2005033099A3 (en) Novel dipeptidyl peptidase iv inhibitors; processes for their preparation and compositions thereof
AU2001266219A1 (en) Novel process for preparing and harvesting crystalline particles
AU2001266218A1 (en) Process for preparing and harvesting crystalline particles
MY139541A (en) Indane acetic acid derivatives and their use as pharmaceutical agents, intermediates, and method of preparation
AU2003251658A1 (en) Purification process of carbon nanotubes
IL165988A0 (en) Process for the preparation of fibrinogen
AU2003279186A1 (en) Process of purifying phosphoramidites
AU2001212914A1 (en) Friedel-craft process for the preparation of thioxanthones
WO2004075860A3 (en) Process for purification of zoledronic acid
AU2003288553A1 (en) Process for purifying oligonucleotide synthons
AU2002358767A1 (en) Purification process of aromatics
AU2001289772A1 (en) Method for purifying 2-chloro-5-chloromethyl thiazole
AU7652100A (en) Method for extracting bacterio-rhodopsin
AU2003300727A1 (en) Process for synthesis of alpha-substituted acroleins
AU2003227622A1 (en) Methylthiophene carboxanilides

Legal Events

Date Code Title Description
MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase