JP4723254B2 - プラスミドｄｎａを精製するための方法 - Google Patents

プラスミドｄｎａを精製するための方法 Download PDF

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JP4723254B2
JP4723254B2 JP2004565105A JP2004565105A JP4723254B2 JP 4723254 B2 JP4723254 B2 JP 4723254B2 JP 2004565105 A JP2004565105 A JP 2004565105A JP 2004565105 A JP2004565105 A JP 2004565105A JP 4723254 B2 JP4723254 B2 JP 4723254B2
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dna
dna product
plasmid dna
plasmid
host cell
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JP2006511228A (ja
JP2006511228A5 (enExample
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ブダハジー，グレッグ
ゴフ，ブレイク
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バイカル インコーポレイテッド
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
JP2004565105A 2002-12-23 2003-11-24 プラスミドｄｎａを精製するための方法 Expired - Fee Related JP4723254B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US43527002P 2002-12-23 2002-12-23
US60/435,270 2002-12-23
PCT/US2003/037665 WO2004060277A2 (en) 2002-12-23 2003-11-24 Process for purification of plasmid dna

Publications (3)

Publication Number Publication Date
JP2006511228A JP2006511228A (ja) 2006-04-06
JP2006511228A5 JP2006511228A5 (enExample) 2007-01-18
JP4723254B2 true JP4723254B2 (ja) 2011-07-13

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JP2004565105A Expired - Fee Related JP4723254B2 (ja) 2002-12-23 2003-11-24 プラスミドｄｎａを精製するための方法

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US (2) US20040157244A1 (enExample)
EP (1) EP1578763B1 (enExample)
JP (1) JP4723254B2 (enExample)
AT (1) ATE420167T1 (enExample)
AU (1) AU2003293042A1 (enExample)
CA (1) CA2509337C (enExample)
DE (1) DE60325773D1 (enExample)
WO (1) WO2004060277A2 (enExample)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1575505A4 (en) * 2002-09-10 2007-01-24 Vical Inc CODON OPTIMIZATION-BASED POLYNUCLEOTIDE VACCINES AGAINST BACILLUS ANTHRACIS INFECTION
US8080642B2 (en) * 2003-05-16 2011-12-20 Vical Incorporated Severe acute respiratory syndrome DNA compositions and methods of use
CA2536931A1 (en) * 2003-09-17 2005-03-24 Centelion Method of preparation of pharmaceutically grade plasmid dna
CN102031258A (zh) * 2004-04-19 2011-04-27 艾文蒂斯药品公司 纯化质粒dna的方法
CA2566355C (en) 2004-05-18 2014-04-15 Vical Incorporated Influenza virus vaccine composition and methods of use
JP2006067890A (ja) * 2004-09-01 2006-03-16 Shigeji Ikeda 核酸抽出方法および核酸抽出キット
KR100624450B1 (ko) * 2004-12-10 2006-09-18 삼성전자주식회사 히드로겔을 이용한 생물분자의 분리 및 정제 방법
US20070178578A1 (en) * 2006-01-27 2007-08-02 Nesa & Associates, Inc. Biofiltration system for treating airborne volatile organic compounds
CN100439497C (zh) * 2006-05-26 2008-12-03 吉林大学 一种适用于大规模生产质粒dna的纯化方法
WO2013067069A2 (en) * 2011-10-31 2013-05-10 Leinweber Barbara Dual digestion method for high yield nucleic acid recovery
EP2989116A4 (en) * 2013-04-26 2017-01-18 Nitto Denko Corporation Reduction of endotoxins from polyanionic polymer conjugates
IL296722A (en) 2020-03-31 2022-11-01 Richter Helm Biologics Gmbh & Co Kg Methods for the production of plasmid DNA
EP3906982B2 (de) * 2020-05-08 2025-08-13 AXAGARIUS GmbH & Co. KG Verfahren zur plasmidreinigung unter gleichzeitiger abreicherung von endotoxinen
WO2022073995A1 (en) * 2020-10-05 2022-04-14 Bia Separations D.O.O. Improved removal of rna and contaminants from dna plasmid preparations by hydrophobic interaction chromatography
WO2022231930A1 (en) 2021-04-26 2022-11-03 Celanese Eva Performance Polymers Llc Implantable device for sustained release of a macromolecular drug compound
GB202202777D0 (en) * 2022-03-01 2022-04-13 Cytiva Bioprocess R & D Ab A method for separating supercoiled plasmid DNA
FR3143127A1 (fr) * 2022-12-07 2024-06-14 Commissariat à l'Energie Atomique et aux Energies Alternatives Procédé de détection d'une espèce allergène, contaminante ou de nature frauduleuse dans un échantillon agro-alimentaire
WO2025157970A1 (en) 2024-01-26 2025-07-31 Richter Biologics Gmbh & Co. Kg Large scale methods for producing plasmid dna

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4900677A (en) * 1986-09-26 1990-02-13 E. I. Du Pont De Nemours And Company Process for rapid isolation of high molecular weight DNA
US4935342A (en) * 1986-12-01 1990-06-19 Syngene, Inc. Method of isolating and purifying nucleic acids from biological samples
CA1297431C (en) * 1987-04-24 1992-03-17 F. Hoffmann-La Roche Ag Process for the isolation of nucleic acids
US4923978A (en) * 1987-12-28 1990-05-08 E. I. Du Pont De Nemours & Company Process for purifying nucleic acids
US5445732A (en) * 1992-06-19 1995-08-29 Sepracor Inc. Passivated porous polymer supports and methods for the preparation and use of same
US6087491A (en) * 1993-01-08 2000-07-11 Hybridon, Inc. Extremely high purity oligonucleotides and methods of synthesizing them using dimer blocks
US5561064A (en) * 1994-02-01 1996-10-01 Vical Incorporated Production of pharmaceutical-grade plasmid DNA
ES2227557T3 (es) * 1994-07-15 2005-04-01 MERCK & CO., INC. Procedimiento para la purificacion a gran escala de plasmidos.
US5576196A (en) * 1995-01-13 1996-11-19 Vical Incorporated Process for reducing RNA concentration in a mixture of biological material using diatomaceous earth
US7026468B2 (en) * 1996-07-19 2006-04-11 Valentis, Inc. Process and equipment for plasmid purification
US6011148A (en) * 1996-08-01 2000-01-04 Megabios Corporation Methods for purifying nucleic acids
US7807822B2 (en) * 1996-08-01 2010-10-05 Robert Bridenbaugh Methods for purifying nucleic acids
US5707812A (en) * 1996-08-06 1998-01-13 Vical Incorporated Purification of plasmid DNA during column chromatography
US6214586B1 (en) * 1997-12-08 2001-04-10 Genzyme Corporation Method for purifying plasmid DNA and plasmid DNA substantially free of genomic DNA
US6441160B2 (en) * 1998-05-11 2002-08-27 Tosoh Corporation Separating plasmids from contaminants using hydrophobic or hydrophobic and ion exchange chromatography
GB9907553D0 (en) * 1999-04-01 1999-05-26 Cantab Pharma Res Purification of biological preparations
DE60036226T2 (de) * 1999-12-22 2008-05-21 Merck & Co., Inc. Verfahren zur skalierbaren reinigung von plasmid-dns
GB0107634D0 (en) * 2001-03-27 2001-05-16 Fermentas Ab Nucleic acid purification

Also Published As

Publication number Publication date
EP1578763A2 (en) 2005-09-28
AU2003293042A8 (en) 2004-07-29
US20040157244A1 (en) 2004-08-12
AU2003293042A1 (en) 2004-07-29
DE60325773D1 (de) 2009-02-26
US20100273254A1 (en) 2010-10-28
CA2509337A1 (en) 2004-07-22
WO2004060277A2 (en) 2004-07-22
ATE420167T1 (de) 2009-01-15
CA2509337C (en) 2013-01-22
WO2004060277A3 (en) 2005-02-17
EP1578763A4 (en) 2006-12-06
JP2006511228A (ja) 2006-04-06
EP1578763B1 (en) 2009-01-07

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