ATE290604T1 - Methode zum nachweis von atp - Google Patents
Methode zum nachweis von atpInfo
- Publication number
- ATE290604T1 ATE290604T1 AT99949447T AT99949447T ATE290604T1 AT E290604 T1 ATE290604 T1 AT E290604T1 AT 99949447 T AT99949447 T AT 99949447T AT 99949447 T AT99949447 T AT 99949447T AT E290604 T1 ATE290604 T1 AT E290604T1
- Authority
- AT
- Austria
- Prior art keywords
- sample
- detecting atp
- luciferin
- luciferase
- light signal
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 2
- 150000003839 salts Chemical class 0.000 abstract 4
- 239000005089 Luciferase Substances 0.000 abstract 3
- IGXWBGJHJZYPQS-SSDOTTSWSA-N D-Luciferin Chemical compound OC(=O)[C@H]1CSC(C=2SC3=CC=C(O)C=C3N=2)=N1 IGXWBGJHJZYPQS-SSDOTTSWSA-N 0.000 abstract 2
- CYCGRDQQIOGCKX-UHFFFAOYSA-N Dehydro-luciferin Natural products OC(=O)C1=CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 CYCGRDQQIOGCKX-UHFFFAOYSA-N 0.000 abstract 2
- BJGNCJDXODQBOB-UHFFFAOYSA-N Fivefly Luciferin Natural products OC(=O)C1CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 BJGNCJDXODQBOB-UHFFFAOYSA-N 0.000 abstract 2
- 108060001084 Luciferase Proteins 0.000 abstract 2
- DDWFXDSYGUXRAY-UHFFFAOYSA-N Luciferin Natural products CCc1c(C)c(CC2NC(=O)C(=C2C=C)C)[nH]c1Cc3[nH]c4C(=C5/NC(CC(=O)O)C(C)C5CC(=O)O)CC(=O)c4c3C DDWFXDSYGUXRAY-UHFFFAOYSA-N 0.000 abstract 2
- 239000011541 reaction mixture Substances 0.000 abstract 2
- 239000007853 buffer solution Substances 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/902—Oxidoreductases (1.)
- G01N2333/90241—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/968—High energy substrates, e.g. fluorescent, chemiluminescent, radioactive
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/975—Kit
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| NL1010224A NL1010224C2 (nl) | 1998-09-30 | 1998-09-30 | Werkwijze voor het detecteren van ATP. |
| PCT/NL1999/000606 WO2000018953A1 (en) | 1998-09-30 | 1999-09-30 | Method for detecting atp |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE290604T1 true ATE290604T1 (de) | 2005-03-15 |
Family
ID=19767908
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT99949447T ATE290604T1 (de) | 1998-09-30 | 1999-09-30 | Methode zum nachweis von atp |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US6503723B1 (de) |
| EP (1) | EP1117825B2 (de) |
| JP (1) | JP4637357B2 (de) |
| AT (1) | ATE290604T1 (de) |
| AU (1) | AU754602B2 (de) |
| CA (1) | CA2345721C (de) |
| DE (1) | DE69924127T3 (de) |
| IL (1) | IL142003A (de) |
| NL (1) | NL1010224C2 (de) |
| WO (1) | WO2000018953A1 (de) |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0030727D0 (en) | 2000-12-15 | 2001-01-31 | Lumitech Uk Ltd | Methods and kits for detecting kinase activity |
| US7083911B2 (en) | 2001-02-16 | 2006-08-01 | Promega Corporation | Method for detection of ATP |
| DE60334923D1 (de) | 2002-09-06 | 2010-12-23 | Promega Corp | Tät |
| JP4485470B2 (ja) | 2002-12-23 | 2010-06-23 | プロメガ コーポレイション | ルシフェラーゼ酵素活性を保護するための方法及びキット |
| AU2003212402A1 (en) * | 2003-03-20 | 2004-10-11 | Csi Biotech Oy | Amp ligation assay (ala) |
| US7132249B1 (en) | 2003-05-12 | 2006-11-07 | Charm Sciences, Inc. | Method of determining allergenic food on surfaces |
| US7494781B1 (en) | 2003-05-12 | 2009-02-24 | Charm Sciences, Inc. | Sensitive method for detecting low levels of ATP |
| RU2268943C2 (ru) * | 2004-02-19 | 2006-01-27 | Наталья Николаевна Угарова | Реагент для определения аденозин-5'-трифосфата |
| CA2572608A1 (en) * | 2004-07-02 | 2006-08-17 | Promega Corporation | Microbial atp extraction and detection system |
| JP4682310B2 (ja) * | 2004-12-28 | 2011-05-11 | 独立行政法人産業技術総合研究所 | 天然型ｌ−システインまたはその誘導体を用いたホタル発光基質の生合成システム及び本システムを含んだ発光基質溶液 |
| EP1724359A1 (de) * | 2005-05-18 | 2006-11-22 | PerkinElmer Life and Analytical Sciences B.V. | Luciferase-Assaysystem |
| GB0513535D0 (en) * | 2005-07-01 | 2005-08-10 | Iseao Technologies Ltd | Improved methods of detecting microbes |
| EP1945257A4 (de) * | 2005-08-08 | 2009-05-06 | Onconon Llc | Antikörperzusammensetzungen, verfahren zur behandlung von neoplastischer erkrankung und verfahren zur regulierung der fertilität |
| CN101283090A (zh) * | 2005-08-19 | 2008-10-08 | 联邦科学及工业研究组织 | 菌蚋荧光素酶 |
| EP1955077B1 (de) | 2005-12-02 | 2012-06-13 | Sirtris Pharmaceuticals, Inc. | Massen-spektronomie-tests für die acetyltransferase-deacetylase-aktivität |
| KR100801975B1 (ko) | 2006-06-29 | 2008-02-12 | 심재희 | 병원균의 유무를 측정하는 고감도 실시간 에이티피측정장치 |
| WO2008045121A1 (en) * | 2006-10-13 | 2008-04-17 | Charm Sciences, Inc. | Method and apparatus for reducing luminescent test result interferences |
| US8232047B2 (en) * | 2006-10-24 | 2012-07-31 | Gene Stream Pty Ltd. | Luciferase signal enhancing compositions |
| KR101478373B1 (ko) | 2006-12-21 | 2014-12-31 | 진 스트림 피티와이 리미티드 | 분비성 루시페라아제를 이용하는 생물 발광 검사 |
| AU2010247461B2 (en) * | 2009-05-12 | 2015-09-03 | Janssen Sciences Ireland Uc | Method for identifying inhibitors against dengue virus |
| WO2011056611A1 (en) | 2009-10-26 | 2011-05-12 | Myrexis, Inc. | Coupled reactions for analysis of nucleotides and their hydrolysis |
| EP2646548B1 (de) * | 2010-12-03 | 2017-03-22 | Gene Stream Pty Ltd. | Verbesserte lichtemittierende moleküle |
| CN110607347A (zh) * | 2019-10-30 | 2019-12-24 | 浙江东鸿生物科技有限公司 | Atp荧光检测试剂 |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SE428379B (sv) * | 1978-05-31 | 1983-06-27 | Lkb Produkter Ab | Bioluminiscens bestemning av atp och reagens herfor |
| GB2116709B (en) * | 1982-03-09 | 1986-02-19 | Wira & Mather | Microbiological testing detecting surface mildew |
| US5283179A (en) * | 1990-09-10 | 1994-02-01 | Promega Corporation | Luciferase assay method |
| GB9100551D0 (en) * | 1991-01-10 | 1991-02-20 | Amersham Int Plc | Method and reagent for eliminating analytical interference in enzymatic analysis from substances used for extraction of intracellular metabolites |
| DE4212555A1 (de) * | 1992-04-15 | 1993-10-21 | Boehringer Mannheim Gmbh | Verfahren zum Nachweis von Nukleinsäuren |
| AT401526B (de) * | 1993-02-10 | 1996-09-25 | Scheirer Winfried | Reagenzlösung zur stabilisierung der lumineszenz bei der luciferasemessung |
| US5561064A (en) * | 1994-02-01 | 1996-10-01 | Vical Incorporated | Production of pharmaceutical-grade plasmid DNA |
| JPH0847399A (ja) * | 1994-08-08 | 1996-02-20 | Eiken Chem Co Ltd | 生物発光の測定方法 |
| US5744320A (en) * | 1995-06-07 | 1998-04-28 | Promega Corporation | Quenching reagents and assays for enzyme-mediated luminescence |
| DE19703437A1 (de) * | 1997-01-30 | 1998-08-06 | Luitpold Pharma Gmbh | Gemische äußerer Membranen und/oder Zellwände von Bakterien zur oralen Immunisierung gegen Schleimhautinfektionen |
| JPH10236985A (ja) * | 1997-02-26 | 1998-09-08 | Taiho Yakuhin Kogyo Kk | 遺伝子治療用製剤 |
-
1998
- 1998-09-30 NL NL1010224A patent/NL1010224C2/nl not_active IP Right Cessation
-
1999
- 1999-09-30 AU AU62319/99A patent/AU754602B2/en not_active Expired
- 1999-09-30 WO PCT/NL1999/000606 patent/WO2000018953A1/en not_active Ceased
- 1999-09-30 IL IL14200399A patent/IL142003A/xx not_active IP Right Cessation
- 1999-09-30 JP JP2000572400A patent/JP4637357B2/ja not_active Expired - Lifetime
- 1999-09-30 EP EP99949447A patent/EP1117825B2/de not_active Expired - Lifetime
- 1999-09-30 DE DE69924127T patent/DE69924127T3/de not_active Expired - Lifetime
- 1999-09-30 US US09/806,165 patent/US6503723B1/en not_active Expired - Lifetime
- 1999-09-30 AT AT99949447T patent/ATE290604T1/de active
- 1999-09-30 CA CA2345721A patent/CA2345721C/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| CA2345721C (en) | 2011-01-11 |
| DE69924127T3 (de) | 2010-01-14 |
| DE69924127D1 (de) | 2005-04-14 |
| AU754602B2 (en) | 2002-11-21 |
| EP1117825A1 (de) | 2001-07-25 |
| IL142003A (en) | 2005-08-31 |
| US6503723B1 (en) | 2003-01-07 |
| EP1117825B1 (de) | 2005-03-09 |
| IL142003A0 (en) | 2002-03-10 |
| NL1010224C2 (nl) | 2000-03-31 |
| CA2345721A1 (en) | 2000-04-06 |
| EP1117825B2 (de) | 2009-06-24 |
| JP2002525123A (ja) | 2002-08-13 |
| WO2000018953A1 (en) | 2000-04-06 |
| JP4637357B2 (ja) | 2011-02-23 |
| DE69924127T2 (de) | 2006-04-06 |
| AU6231999A (en) | 2000-04-17 |
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