ATE269900T1 - Verfahren zur molekularen evolution der antikörperfunktion - Google Patents

Verfahren zur molekularen evolution der antikörperfunktion

Info

Publication number
ATE269900T1
ATE269900T1 AT01925538T AT01925538T ATE269900T1 AT E269900 T1 ATE269900 T1 AT E269900T1 AT 01925538 T AT01925538 T AT 01925538T AT 01925538 T AT01925538 T AT 01925538T AT E269900 T1 ATE269900 T1 AT E269900T1
Authority
AT
Austria
Prior art keywords
framework
molecular evolution
nucleic acid
acid encoding
present
Prior art date
Application number
AT01925538T
Other languages
English (en)
Inventor
Mats Ohlin
Eskil Soderlind
Roland Carlsson
Original Assignee
Bioinvent Int Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bioinvent Int Ab filed Critical Bioinvent Int Ab
Application granted granted Critical
Publication of ATE269900T1 publication Critical patent/ATE269900T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B10/00—Directed molecular evolution of macromolecules, e.g. RNA, DNA or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/565—Complementarity determining region [CDR]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/567—Framework region [FR]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Ecology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Polyesters Or Polycarbonates (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT01925538T 2000-04-05 2001-04-04 Verfahren zur molekularen evolution der antikörperfunktion ATE269900T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0008419.4A GB0008419D0 (en) 2000-04-05 2000-04-05 A method for invitro molecular evolution of antibody function
PCT/EP2001/004065 WO2001075091A2 (en) 2000-04-05 2001-04-04 A method for in vitro molecular evolution of antibody function

Publications (1)

Publication Number Publication Date
ATE269900T1 true ATE269900T1 (de) 2004-07-15

Family

ID=9889301

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01925538T ATE269900T1 (de) 2000-04-05 2001-04-04 Verfahren zur molekularen evolution der antikörperfunktion

Country Status (11)

Country Link
US (2) US20030153038A1 (de)
EP (1) EP1268801B1 (de)
JP (1) JP4842490B2 (de)
AT (1) ATE269900T1 (de)
AU (2) AU2001252253B2 (de)
CA (1) CA2402240C (de)
DE (1) DE60103988T2 (de)
GB (1) GB0008419D0 (de)
IL (2) IL151743A0 (de)
NO (1) NO330262B1 (de)
WO (1) WO2001075091A2 (de)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1457559B1 (de) 2001-11-22 2008-01-16 Keio University Künstliche antikörperbibliothek mit super-repertoire
US7135310B2 (en) 2002-04-24 2006-11-14 The Regents Of The University Of California Method to amplify variable sequences without imposing primer sequences
AU2003238370B2 (en) 2002-05-22 2010-04-29 Novartis Ag Immunoglobulin frameworks which demonstrate enhanced stability in the intracellular environment and methods of identifying same
GB2400851B (en) * 2003-04-25 2004-12-15 Bioinvent Int Ab Identifying binding of a polypeptide to a polypeptide target
DE602004032042D1 (de) * 2003-08-27 2011-05-12 Proterec Ltd Bibliotheken rekombinanter chimärischer proteine
EP1704251A1 (de) * 2003-12-15 2006-09-27 Institut Pasteur Repertoirbestimmung einer b-lymphozytenpopulation
EP1544308B1 (de) * 2003-12-15 2009-01-28 Institut Pasteur Ermittlung des Repertoires von B-Lymphozyten Populationen
GB0500703D0 (en) * 2005-01-14 2005-02-23 Bioinvent Int Ab Molecular biology method
BRPI0807952A2 (pt) * 2007-02-20 2014-06-10 Anaptysbio Inc Sistemas de hipermutação somática
DK2356270T3 (da) 2008-11-07 2016-12-12 Fabrus Llc Kombinatoriske antistofbiblioteker og anvendelser deraf
CA2780221A1 (en) 2009-11-04 2011-05-12 Fabrus Llc Methods for affinity maturation-based antibody optimization
CN105531370A (zh) 2013-04-23 2016-04-27 阿伯丁大学理事会 治疗靶向特异性vnar结构域与icosl的分离
EP3561703B1 (de) * 2018-04-25 2021-01-20 Bayer AG Identifizieren der paarung von variablen domänen aus leichten und schweren ketten von antikörpern
WO2020014143A1 (en) 2018-07-08 2020-01-16 Specifica Inc. Antibody libraries with maximized antibody developability characteristics

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4440859A (en) * 1977-05-27 1984-04-03 The Regents Of The University Of California Method for producing recombinant bacterial plasmids containing the coding sequences of higher organisms
US4704362A (en) * 1977-11-08 1987-11-03 Genentech, Inc. Recombinant cloning vehicle microbial polypeptide expression
NO794196L (no) * 1978-12-22 1980-06-24 Biogen Nv Fremgangsmaate ved fremstilling av et rekombinert dna molekyl
US4530901A (en) * 1980-01-08 1985-07-23 Biogen N.V. Recombinant DNA molecules and their use in producing human interferon-like polypeptides
US4678751A (en) * 1981-09-25 1987-07-07 Genentech, Inc. Hybrid human leukocyte interferons
US4766075A (en) * 1982-07-14 1988-08-23 Genentech, Inc. Human tissue plasminogen activator
US4582800A (en) * 1982-07-12 1986-04-15 Hoffmann-La Roche Inc. Novel vectors and method for controlling interferon expression
US4757006A (en) * 1983-10-28 1988-07-12 Genetics Institute, Inc. Human factor VIII:C gene and recombinant methods for production
US4677063A (en) * 1985-05-02 1987-06-30 Cetus Corporation Human tumor necrosis factor
US4810648A (en) * 1986-01-08 1989-03-07 Rhone Poulenc Agrochimie Haloarylnitrile degrading gene, its use, and cells containing the gene
US5565332A (en) * 1991-09-23 1996-10-15 Medical Research Council Production of chimeric antibodies - a combinatorial approach
CA2229043C (en) * 1995-08-18 2016-06-07 Morphosys Gesellschaft Fur Proteinoptimierung Mbh Protein/(poly)peptide libraries
GB9701425D0 (en) * 1997-01-24 1997-03-12 Bioinvent Int Ab A method for in vitro molecular evolution of protein function
EP1007967A2 (de) * 1997-08-04 2000-06-14 Ixsys, Incorporated Verfahren zur identifizierung ligandspezifischer bindungsmoleküle

Also Published As

Publication number Publication date
US20120077710A1 (en) 2012-03-29
DE60103988D1 (de) 2004-07-29
EP1268801A2 (de) 2003-01-02
AU2001252253B2 (en) 2004-04-08
CA2402240C (en) 2012-02-28
JP4842490B2 (ja) 2011-12-21
JP2003529368A (ja) 2003-10-07
WO2001075091A3 (en) 2002-04-18
GB0008419D0 (en) 2000-05-24
IL151743A (en) 2009-02-11
AU5225301A (en) 2001-10-15
WO2001075091A2 (en) 2001-10-11
IL151743A0 (en) 2003-04-10
CA2402240A1 (en) 2001-10-11
EP1268801B1 (de) 2004-06-23
NO20024723L (no) 2002-12-02
DE60103988T2 (de) 2005-07-14
NO20024723D0 (no) 2002-10-02
US20030153038A1 (en) 2003-08-14
NO330262B1 (no) 2011-03-14

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