ATE251174T1 - Synthese von markierten oligonucleotiden auf festphasenträgern - Google Patents

Synthese von markierten oligonucleotiden auf festphasenträgern

Info

Publication number
ATE251174T1
ATE251174T1 AT00911866T AT00911866T ATE251174T1 AT E251174 T1 ATE251174 T1 AT E251174T1 AT 00911866 T AT00911866 T AT 00911866T AT 00911866 T AT00911866 T AT 00911866T AT E251174 T1 ATE251174 T1 AT E251174T1
Authority
AT
Austria
Prior art keywords
solid
support
label
acid
labelled
Prior art date
Application number
AT00911866T
Other languages
English (en)
Inventor
Ravi S Vinayak
Linda G Lee
Khairuzzaman B Mullah
Barnett B Rosenblum
Original Assignee
Applera Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Applera Corp filed Critical Applera Corp
Application granted granted Critical
Publication of ATE251174T1 publication Critical patent/ATE251174T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
AT00911866T 1999-02-22 2000-02-18 Synthese von markierten oligonucleotiden auf festphasenträgern ATE251174T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/256,340 US6255476B1 (en) 1999-02-22 1999-02-22 Methods and compositions for synthesis of labelled oligonucleotides and analogs on solid-supports
PCT/US2000/004213 WO2000050432A2 (en) 1999-02-22 2000-02-18 Synthesis of labelled oligonucleotides on solid-supports

Publications (1)

Publication Number Publication Date
ATE251174T1 true ATE251174T1 (de) 2003-10-15

Family

ID=22971883

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00911866T ATE251174T1 (de) 1999-02-22 2000-02-18 Synthese von markierten oligonucleotiden auf festphasenträgern

Country Status (10)

Country Link
US (5) US6255476B1 (de)
EP (1) EP1155027B1 (de)
JP (4) JP4634615B2 (de)
AT (1) ATE251174T1 (de)
AU (1) AU773864B2 (de)
CA (1) CA2353047C (de)
DE (1) DE60005646T2 (de)
DK (1) DK1155027T3 (de)
ES (1) ES2204537T3 (de)
WO (1) WO2000050432A2 (de)

Families Citing this family (95)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6881570B1 (en) * 1998-12-15 2005-04-19 Fuji Photo Film Co., Ltd. Test piece and quantitative method and apparatus for an organism-oriented substance
US6255476B1 (en) * 1999-02-22 2001-07-03 Pe Corporation (Ny) Methods and compositions for synthesis of labelled oligonucleotides and analogs on solid-supports
JP3957118B2 (ja) * 1999-05-18 2007-08-15 富士フイルム株式会社 試験片およびこの試験片からの画像情報読取装置
AU5285800A (en) 1999-05-24 2000-12-12 Invitrogen Corporation Method for deblocking of labeled oligonucleotides
US7183405B2 (en) * 1999-06-25 2007-02-27 Syn Gen, Inc. Compositions and methods for labeling oligonucleotides
US7211390B2 (en) 1999-09-16 2007-05-01 454 Life Sciences Corporation Method of sequencing a nucleic acid
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
US6451588B1 (en) * 2000-06-30 2002-09-17 Pe Corporation (Ny) Multipartite high-affinity nucleic acid probes
JP3398366B2 (ja) * 2000-08-09 2003-04-21 富士写真フイルム株式会社 Ｄｎａ分析用マイクロアレイの製造方法
US6559279B1 (en) 2000-09-08 2003-05-06 Isis Pharmaceuticals, Inc. Process for preparing peptide derivatized oligomeric compounds
DE60140317D1 (de) * 2000-12-14 2009-12-10 Gen Probe Inc Verfahren und kits zur verbesserung der hybridisierungsraten von polynukleotiden
US6534646B2 (en) 2001-06-04 2003-03-18 Barrskogen, Inc. Oligonucleotide labeling reagents
US6448016B1 (en) * 2001-06-12 2002-09-10 The United States Of America As Represented By The Secretary Of The Army Nucleotide sequences for detection of Bacillus anthracis
US20050009022A1 (en) * 2001-07-06 2005-01-13 Weiner Michael P. Method for isolation of independent, parallel chemical micro-reactions using a porous filter
US20030054396A1 (en) * 2001-09-07 2003-03-20 Weiner Michael P. Enzymatic light amplification
US6902921B2 (en) 2001-10-30 2005-06-07 454 Corporation Sulfurylase-luciferase fusion proteins and thermostable sulfurylase
US6956114B2 (en) 2001-10-30 2005-10-18 '454 Corporation Sulfurylase-luciferase fusion proteins and thermostable sulfurylase
US20050124022A1 (en) * 2001-10-30 2005-06-09 Maithreyan Srinivasan Novel sulfurylase-luciferase fusion proteins and thermostable sulfurylase
US6946245B2 (en) 2001-12-04 2005-09-20 Quest Diagnostics Investments Incorporated Oligonucleotides and methods for detecting hepatitis C viral nucleic acids
US6686162B2 (en) 2001-12-04 2004-02-03 Quest Diagnostics Investments, Incorporated Oligonucleotides and methods for detecting Borrelia burgdorferi
DE10206616A1 (de) * 2002-02-15 2003-09-04 Qiagen Gmbh Verfahren zur Reduktion der Background-Kontamination nach Markierungsreaktionen
CA2498320A1 (en) * 2002-09-20 2004-04-01 Integrated Dna Technologies, Inc. Anthraquinone quencher dyes, their methods of preparation and use
US6913890B2 (en) * 2002-12-18 2005-07-05 Palo Alto Research Center Incorporated Process for preparing albumin protein conjugated oligonucleotide probes
US20060292438A1 (en) * 2002-12-23 2006-12-28 Applera Corporation; Applied Biosystems Group Heteroconfigurational Polynucleotides and Methods of Use
US20080015349A1 (en) * 2003-01-24 2008-01-17 Third Wave Technologies, Inc. Oligonucleotide production
JP4480715B2 (ja) * 2003-01-29 2010-06-16 ４５４ コーポレーション 二重末端シーケンシング
US7575865B2 (en) * 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
EP1466919A1 (de) * 2003-04-05 2004-10-13 Roche Diagnostics GmbH Sechsgliedrige Ringe Nukleotidanalogue
CA2463719A1 (en) * 2003-04-05 2004-10-05 F. Hoffmann-La Roche Ag Nucleotide analogs with six membered rings
US7439341B2 (en) * 2003-11-14 2008-10-21 Integrated Dna Technologies, Inc. Fluorescence quenching azo dyes, their methods of preparation and use
US20050153300A1 (en) * 2004-01-09 2005-07-14 Quest Diagnostics Incorporated Methods and compositions for the detection of mucolipidosis IV mutations
US20050214779A1 (en) * 2004-03-29 2005-09-29 Peck Bill J Methods for in situ generation of nucleic acid arrays
EP1627926A1 (de) * 2004-08-19 2006-02-22 Roche Diagnostics GmbH Eine Methode zur Reduktion von falsch-positiven Messergebnissen
WO2006052842A2 (en) 2004-11-09 2006-05-18 The Trustees Of The University Of Pennsylvania Methods for diagnosis of myelodysplastic syndromes (mds)
CA2601554A1 (en) * 2005-05-20 2006-11-30 Integrated Dna Technologies, Inc. Compounds and methods for labeling oligonucleotides
WO2007024798A2 (en) 2005-08-22 2007-03-01 Applera Corporation Apparatus, system, and method using immiscible-fluid-discrete-volumes
ES2332139T3 (es) 2005-11-23 2010-01-27 F. Hoffmann-La Roche Ag Polinucleotidos con mimetico de fosfato.
WO2007106907A2 (en) * 2006-03-15 2007-09-20 Integrated Dna Technologies, Inc. Nucleic acid monomers with 2'-chemical moieties
EP2001871B1 (de) * 2006-03-31 2014-07-16 Applied Biosystems, LLC Rhodamin-markierte oligonukleotide
CA2649770C (en) 2006-04-18 2017-08-15 The Trustees Of The University Of Pennsylvania Mutated acvr1 for diagnosis and treatment of fibrodysplasia ossificans progressiva (fop)
US8859752B2 (en) 2006-04-18 2014-10-14 The Trustees Of The University Of Pennsylvania SIRNA-based therapy of Fibrodyplasia Ossificans Progressiva (FOP)
US7759062B2 (en) * 2006-06-09 2010-07-20 Third Wave Technologies, Inc. T-structure invasive cleavage assays, consistent nucleic acid dispensing, and low level target nucleic acid detection
US20080153135A1 (en) * 2006-12-20 2008-06-26 Liu Timothy Z Methods and apparatus for conducting amplification reactions on high density hydrophilic patterned microplates
US20080153134A1 (en) * 2006-12-22 2008-06-26 Willy Wiyatno Methods and apparatus for generating hydrophilic patterning of high density microplates using an amphiphilic polymer
US20080268440A1 (en) * 2007-04-26 2008-10-30 Liu Timothy Z Biomolecule immobilization on surface via hydrophobic interactions
EP2036897B1 (de) * 2007-09-04 2017-10-18 Roche Diagnostics GmbH Stabiles Reagens zur Rhodaminmarkierung
JP2010540900A (ja) 2007-09-18 2010-12-24 アプライド バイオシステムズ インコーポレイテッド 集光機構のための方法、システムおよび装置
US20090139311A1 (en) * 2007-10-05 2009-06-04 Applied Biosystems Inc. Biological Analysis Systems, Devices, and Methods
JP5365005B2 (ja) * 2008-01-17 2013-12-11 富士通株式会社 中性で切断可能な核酸合成用レジン
WO2009117327A2 (en) 2008-03-15 2009-09-24 Hologic, Inc. Compositions and methods for analysis of nucleic acid molecules during amplification reactions
JP5438922B2 (ja) * 2008-06-25 2014-03-12 日東電工株式会社 核酸の製造方法
US8097412B2 (en) * 2008-07-12 2012-01-17 Biodiagnostics, Inc. DNA-based test for detection of annual and intermediate ryegrass
EP2192198A1 (de) 2008-12-01 2010-06-02 Biotype AG Neuartige Kombination von Fluoreszenzfarbstoffen zur Erkennung von Nukleinsäuren
US9347092B2 (en) * 2009-02-25 2016-05-24 Roche Molecular System, Inc. Solid support for high-throughput nucleic acid analysis
US20110146418A1 (en) * 2009-10-02 2011-06-23 Brevnov Maxim G Sample Preparation Devices and Methods
WO2011066330A2 (en) * 2009-11-24 2011-06-03 Life Technologies Corporation Selective amplification of polynucleotide sequences
US9506057B2 (en) 2010-03-26 2016-11-29 Integrated Dna Technologies, Inc. Modifications for antisense compounds
EP2553123B1 (de) 2010-03-26 2016-08-24 Integrated DNA Technologies, Inc. Verfahren zur verstärkung einer nukleinsäurehybridisierung
EP3578205A1 (de) 2010-08-06 2019-12-11 ModernaTX, Inc. Pharmazeutische zusammensetzungen enthaltenbearbeitete nukleinsäuren und ihre medizinische verwendung
WO2012033848A1 (en) 2010-09-07 2012-03-15 Integrated Dna Technologies, Inc. Modifications for antisense compounds
HUE058896T2 (hu) 2010-10-01 2022-09-28 Modernatx Inc N1-metil-pszeudo-uracilt tartalmazó ribonukleinsavak és azok felhasználásai
JP2014511687A (ja) 2011-03-31 2014-05-19 モデルナ セラピューティクス インコーポレイテッド 工学操作された核酸の送達および製剤
US9777332B2 (en) 2011-03-31 2017-10-03 St. Jude Children's Research Hospital Methods and compositions for identifying minimal residual disease in acute lymphoblastic leukemia
JP5808025B2 (ja) * 2011-08-12 2015-11-10 国立研究開発法人産業技術総合研究所 アミノ化オリゴヌクレオチド用固相担体
US9464124B2 (en) 2011-09-12 2016-10-11 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
RU2648950C2 (ru) 2011-10-03 2018-04-02 Модерна Терапьютикс, Инк. Модифицированные нуклеозиды, нуклеотиды и нуклеиновые кислоты и их применение
EP2791160B1 (de) 2011-12-16 2022-03-02 ModernaTX, Inc. Modifizierte mrna zusammensetzungen
WO2013119956A1 (en) 2012-02-09 2013-08-15 Life Technologies Corporation Conjugated polymeric particle and method of making same
US9254311B2 (en) 2012-04-02 2016-02-09 Moderna Therapeutics, Inc. Modified polynucleotides for the production of proteins
AU2013243951A1 (en) 2012-04-02 2014-10-30 Moderna Therapeutics, Inc. Modified polynucleotides for the production of secreted proteins
US9283287B2 (en) 2012-04-02 2016-03-15 Moderna Therapeutics, Inc. Modified polynucleotides for the production of nuclear proteins
US9572897B2 (en) 2012-04-02 2017-02-21 Modernatx, Inc. Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins
US8859098B2 (en) * 2012-05-18 2014-10-14 Lord Corporation Acrylic adhesion promoters
EP2906725A4 (de) 2012-10-15 2016-07-20 Sudhir Sinha Verfahren zur genetischen erkennung mit vermischten genetischen elementen: multiplexiertes dna-analysesystem
EP2922554B1 (de) 2012-11-26 2022-02-23 ModernaTX, Inc. Terminal modifizierte rns
WO2014158628A1 (en) 2013-03-14 2014-10-02 Hologic, Inc. Compositions and methods for analysis of nucleic acid molecules
US8980864B2 (en) 2013-03-15 2015-03-17 Moderna Therapeutics, Inc. Compositions and methods of altering cholesterol levels
WO2015034928A1 (en) 2013-09-03 2015-03-12 Moderna Therapeutics, Inc. Chimeric polynucleotides
WO2015048744A2 (en) 2013-09-30 2015-04-02 Moderna Therapeutics, Inc. Polynucleotides encoding immune modulating polypeptides
EA201690675A1 (ru) 2013-10-03 2016-08-31 Модерна Терапьютикс, Инк. Полинуклеотиды, кодирующие рецептор липопротеинов низкой плотности
US20150132256A1 (en) * 2013-10-19 2015-05-14 Trovagene, Inc. Detecting and monitoring mutations in histiocytosis
CA2926722A1 (en) * 2013-10-19 2015-04-23 Trovagene, Inc. Detecting mutations in disease over time
EP3146094B1 (de) * 2014-05-23 2019-08-28 Centrillion Technology Holdings Corporation Oligonukleotidsondeninversionsverfahren für in-situ-synthetisierte sondenarrays
US20170204152A1 (en) 2014-07-16 2017-07-20 Moderna Therapeutics, Inc. Chimeric polynucleotides
WO2016014846A1 (en) 2014-07-23 2016-01-28 Moderna Therapeutics, Inc. Modified polynucleotides for the production of intrabodies
EP3317426B1 (de) 2015-07-02 2020-01-15 Life Technologies Corporation Konjugation von funktionellen hydrophilen carboxylkügelchen
WO2017007774A1 (en) * 2015-07-06 2017-01-12 Life Technologies Corporation Substrates and methods useful in sequencing
US10695735B2 (en) 2015-08-18 2020-06-30 Centrillion Technology Holdings Corporation Probe inversion process for in situ synthesized probe arrays
CN106467913B (zh) * 2015-08-18 2021-07-20 生捷科技控股公司 用于原位合成探针阵列的探针倒转方法
BR112020021218A2 (pt) 2018-04-18 2021-03-02 St. Jude Children's Research Hospital ensaios de genotipagem para identificar mutações em xaf1
US11384376B2 (en) 2018-05-31 2022-07-12 Roche Molecular Systems, Inc. Reagents and methods for post-synthetic modification of nucleic acids
BR112022026056A2 (pt) * 2020-06-22 2023-03-07 Illumina Cambridge Ltd Nucleosídeos e nucleotídeos com grupo bloqueador 3? acetal
KR20230056683A (ko) 2020-07-23 2023-04-27 라이프 테크놀로지스 코포레이션 생물학적 분석에 사용하기 위한 에너지 전달 염료 접합체
CN116323965A (zh) 2020-07-23 2023-06-23 生命技术公司 使用染料的用于生物分析的组合物、系统和方法
CN116333006A (zh) * 2023-04-07 2023-06-27 苏州欧利生物医药科技有限公司 一种带有荧光标记物的寡核苷酸的固相合成方法

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5367066A (en) 1984-10-16 1994-11-22 Chiron Corporation Oligonucleotides with selectably cleavable and/or abasic sites
US4965349A (en) 1987-12-24 1990-10-23 Applied Biosystems, Inc. Method of synthesizing oligonucleotides labeled with ammonia-labile groups on solid phase supports
US5141813A (en) 1989-08-28 1992-08-25 Clontech Laboratories, Inc. Multifunctional controlled pore glass reagent for solid phase oligonucleotide synthesis
US5290925A (en) * 1990-12-20 1994-03-01 Abbott Laboratories Methods, kits, and reactive supports for 3' labeling of oligonucleotides
US5371241A (en) 1991-07-19 1994-12-06 Pharmacia P-L Biochemicals Inc. Fluorescein labelled phosphoramidites
JPH0649100A (ja) * 1992-05-06 1994-02-22 Eastman Kodak Co カルボキシル化基体への化合物の結合方法
US5552471A (en) 1994-08-17 1996-09-03 The Perkin-Elmer Corporation Solid support reagents for the synthesis of 3'-Nitrogen containing polynucleotides
WO1996007667A1 (de) * 1994-09-02 1996-03-14 Novartis Ag Oligonukleotidkonjugate, zusammensetzungen und verfahren zur spaltung von ribonukleinsäuren
US5908926A (en) * 1995-03-16 1999-06-01 Duke University 5'to 3' nucleic acid synthesis using 3'-photoremovable protecting group
US5736626A (en) * 1996-01-29 1998-04-07 The Perkin-Elmer Corporation Solid support reagents for the direct synthesis of 3'-labeled polynucleotides
US6255476B1 (en) * 1999-02-22 2001-07-03 Pe Corporation (Ny) Methods and compositions for synthesis of labelled oligonucleotides and analogs on solid-supports
JP2007161641A (ja) * 2005-12-14 2007-06-28 Pola Chem Ind Inc 液体洗浄料

Also Published As

Publication number Publication date
JP2007131642A (ja) 2007-05-31
US20020165389A1 (en) 2002-11-07
EP1155027A2 (de) 2001-11-21
ES2204537T3 (es) 2004-05-01
CA2353047C (en) 2009-01-20
DE60005646D1 (de) 2003-11-06
AU773864B2 (en) 2004-06-10
US6316610B2 (en) 2001-11-13
WO2000050432A2 (en) 2000-08-31
US20030191303A1 (en) 2003-10-09
JP2007131641A (ja) 2007-05-31
JP4634615B2 (ja) 2011-02-16
WO2000050432A3 (en) 2001-02-01
DK1155027T3 (da) 2003-12-29
JP2002537401A (ja) 2002-11-05
US20050191660A1 (en) 2005-09-01
AU3369100A (en) 2000-09-14
US6255476B1 (en) 2001-07-03
JP2011084573A (ja) 2011-04-28
CA2353047A1 (en) 2000-08-31
EP1155027B1 (de) 2003-10-01
DE60005646T2 (de) 2004-08-05
US6525183B2 (en) 2003-02-25
US20010014735A1 (en) 2001-08-16
US6835827B2 (en) 2004-12-28

Similar Documents

Publication Publication Date Title
ATE251174T1 (de) Synthese von markierten oligonucleotiden auf festphasenträgern
Sinha et al. The preparation and application of functionalised synthetic oligonucleotides: III. Use of H-phosphonate derivatives of protected amino-hexanol and mercapto-propanol or-hexanol
CA1310600C (en) Oligonucleotide functionalizing reagents and methods
CA2627216C (en) Polynucleotide labelling reagent
US4962029A (en) Covalent oligonucleotide-horseradish peroxidase conjugate
EP0954606B1 (de) Nicht-Nukleotid verknüpfende Reagentien
US5916750A (en) Multifunctional linking reagents for synthesis of branched oligomers
US20030036066A1 (en) Linker phosphoramidites for oligonucleotide synthesis
EP0563287A1 (de) Verfahren, testsätze und reaktive träger zur 3'-markierung von oligonukleotiden
EP1152008A3 (de) Fluoreszente Nucleotide, enthaltend einen Cyanin, Merocyanin- oder Styryl-Farbstoff, zur Erkennung von Nucleinsäuren
Thuong et al. Solid phase synthesis of oligo-α-and oligo-β-deoxynucleotidescovalently linked to an acridine
IL127556A0 (en) Enzymatically cleavable linkers for solid-phase syntheses
EP1152010A3 (de) Oligonukleotidmarkierungsstoffe und ihre Verwendung
Vo-Quang et al. Towards new inhibitors of D-alanine: D-alanine ligase: The synthesis of 3-amino butenylphosphonic and aminophosphonamidic acids.
AU2006316903B8 (en) Polynucleotide labelling reagent
US6022714A (en) Methods for attachment of a polynucleotide to a preselected material
DE69823657D1 (de) Zur herstellung von sulfurierten oligonukleotidanaloga
EP1809647A2 (de) Amino- oder thiollinkerbaustein für die synthese von amino- oder thiolfunktionalisierten nukleinsäuren und verfahren zu deren herstellung und anwendung
WO1989002932A1 (en) A covalent oligonucleotide-horseradish peroxidase conjugate
Acedo et al. Preparation of oligonucleotide-dexamethasone conjugates
Semenyuk et al. A base-stable dithiomethyl linker for solid-phase synthesis of oligonucleotides
JPH08511678A (ja) ５’（ｏｈ）および／または３’（ｏｈ）部位に１以上の放射活性のない標識要素を導入するために５’（ｏｈ）および／または３’（ｏｈ）が化学的に修飾された核酸プローブ、およびそれらの製造方法
EP1308452A3 (de) Oligonukleotidmarkierungsreagenzien auf der Basis von azyklischen Nukleosiden, und deren derivatisierte Konjugate
WO2000031102A1 (en) Oligonucleotide conjugation
JP2003171396A (ja) 新規な機能性ペプチド核酸およびその製法

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties