ATE216727T1 - Immobilisierte fehlpaarung - bildendes protein zur detektion oder reinigung von mutationen oder polymorphismen - Google Patents

Immobilisierte fehlpaarung - bildendes protein zur detektion oder reinigung von mutationen oder polymorphismen

Info

Publication number
ATE216727T1
ATE216727T1 AT95901158T AT95901158T ATE216727T1 AT E216727 T1 ATE216727 T1 AT E216727T1 AT 95901158 T AT95901158 T AT 95901158T AT 95901158 T AT95901158 T AT 95901158T AT E216727 T1 ATE216727 T1 AT E216727T1
Authority
AT
Austria
Prior art keywords
mutations
detection
mismatch
base
polymorphisms
Prior art date
Application number
AT95901158T
Other languages
English (en)
Inventor
Robert E Wagner Jr
Original Assignee
Valigene Us Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Valigene Us Inc filed Critical Valigene Us Inc
Application granted granted Critical
Publication of ATE216727T1 publication Critical patent/ATE216727T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6858Allele-specific amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6827Hybridisation assays for detection of mutation or polymorphism
AT95901158T 1993-11-04 1994-11-04 Immobilisierte fehlpaarung - bildendes protein zur detektion oder reinigung von mutationen oder polymorphismen ATE216727T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/147,785 US6027877A (en) 1993-11-04 1993-11-04 Use of immobilized mismatch binding protein for detection of mutations and polymorphisms, purification of amplified DNA samples and allele identification
PCT/US1994/012768 WO1995012689A1 (en) 1993-11-04 1994-11-04 Immobilized mismatch binding protein for detection or purification of mutations or polymorphisms

Publications (1)

Publication Number Publication Date
ATE216727T1 true ATE216727T1 (de) 2002-05-15

Family

ID=22522893

Family Applications (1)

Application Number Title Priority Date Filing Date
AT95901158T ATE216727T1 (de) 1993-11-04 1994-11-04 Immobilisierte fehlpaarung - bildendes protein zur detektion oder reinigung von mutationen oder polymorphismen

Country Status (8)

Country Link
US (2) US6027877A (de)
EP (1) EP0733126B1 (de)
JP (1) JPH09504699A (de)
AT (1) ATE216727T1 (de)
AU (1) AU700959B2 (de)
CA (1) CA2175222A1 (de)
DE (1) DE69430485T2 (de)
WO (1) WO1995012689A1 (de)

Families Citing this family (88)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6120992A (en) 1993-11-04 2000-09-19 Valigene Corporation Use of immobilized mismatch binding protein for detection of mutations and polymorphisms, and allele identification in a diseased human
US6406855B1 (en) 1994-02-17 2002-06-18 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US5877280A (en) * 1995-06-06 1999-03-02 The Mount Sinai School Of Medicine Of The City University Of New York Thermostable muts proteins
EP0870060B1 (de) * 1995-12-15 2003-04-02 Duke University Verfahren zum nachweis und zur entfernung von mutantsequenzen, die während enzymatischer amplifikation entstehen
SE9602062D0 (sv) * 1996-05-29 1996-05-29 Pharmacia Biotech Ab Method for detection of mutations
US6599704B2 (en) 1997-03-04 2003-07-29 Christoph Wagener Method for detecting mutated alleles
DE19708758A1 (de) * 1997-03-04 1998-09-17 Christoph Prof Dr Wagener Verfahren zum Nachweis von mutierten Allelen
JP3318579B2 (ja) 1997-07-07 2002-08-26 理化学研究所 Dnaの塩基配列決定方法
GB9724480D0 (en) * 1997-11-19 1998-01-14 Hexagen Technology Limited Screening process
US6221585B1 (en) * 1998-01-15 2001-04-24 Valigen, Inc. Method for identifying genes underlying defined phenotypes
US6297010B1 (en) 1998-01-30 2001-10-02 Genzyme Corporation Method for detecting and identifying mutations
US6214552B1 (en) * 1998-09-17 2001-04-10 Igen International, Inc. Assays for measuring nucleic acid damaging activities
US6703228B1 (en) * 1998-09-25 2004-03-09 Massachusetts Institute Of Technology Methods and products related to genotyping and DNA analysis
ES2336982T3 (es) * 1998-10-09 2010-04-19 Medical Research Council Procedimiento para la generacion de diversidad.
US7122339B2 (en) 1998-10-09 2006-10-17 Medical Research Council Method for generating diversity
EP1124990B1 (de) 1998-10-27 2006-01-18 Affymetrix, Inc. Komplexitätsmanagement und analyse genomischer dna
DE19911130A1 (de) 1999-03-12 2000-09-21 Hager Joerg Verfahren zur Identifikation chromosomaler Regionen und Gene
US7166432B2 (en) 1999-03-12 2007-01-23 Integragen Compositions and methods for genetic analysis
CA2368875A1 (en) * 1999-04-14 2000-10-19 The Government Of The United States Of America High sensitivity phage display biomolecule detection
JP2000300265A (ja) * 1999-04-19 2000-10-31 Amersham Pharmacia Biotech Kk 2本鎖核酸中のミスマッチ検出方法および変異を有する核酸の検出方法、並びにミスマッチを有する2本鎖核酸の分離方法
US6958225B2 (en) 1999-10-27 2005-10-25 Affymetrix, Inc. Complexity management of genomic DNA
AU2001227679A1 (en) * 2000-02-25 2001-09-03 General Atomics Mutant nucleic binding enzymes and use thereof in diagnostic, detection and purification methods
US6340566B1 (en) * 2000-03-28 2002-01-22 The Regents Of The University Of California Detection and quantitation of single nucleotide polymorphisms, DNA sequence variations, DNA mutations, DNA damage and DNA mismatches
US6365355B1 (en) * 2000-03-28 2002-04-02 The Regents Of The University Of California Chimeric proteins for detection and quantitation of DNA mutations, DNA sequence variations, DNA damage and DNA mismatches
DE60137132D1 (en) * 2000-05-04 2009-02-05 Blood Res Center Are analytische systeme
US20030228600A1 (en) * 2000-07-14 2003-12-11 Eppendorf 5 Prime, Inc. DNA isolation method and kit
DE10038237A1 (de) * 2000-08-04 2002-02-14 Aventis Res & Tech Gmbh & Co Verfahren zum Nachweis von Mutationen in Nucleotidsequenzen
US6632610B2 (en) * 2000-10-12 2003-10-14 Gensat S.A. Methods of identification and isolation of polynucleotides containing nucleic acid differences
EP1368495A4 (de) * 2001-02-19 2005-12-14 Genvartec Pty Ltd Verfahren zur identifizierung von unterschieden zwischen nukleinsäuremolekülen
CA2460496A1 (en) * 2001-09-13 2003-03-20 Genvartec Pty Ltd. Methods of detection of conformational change in a nucleic acid duplex by treatment with oxidising or reactive agent as a result of exposure to environmental or chemical conditions
US20030215837A1 (en) * 2002-01-14 2003-11-20 Diversa Corporation Methods for purifying double-stranded nucleic acids lacking base pair mismatches or nucleotide gaps
US20050130156A1 (en) * 2002-01-14 2005-06-16 Gerhard Frey Methods for making polynucleotides and purifying double-stranded polynucleotides
US20040009498A1 (en) * 2002-01-14 2004-01-15 Diversa Corporation Chimeric antigen binding molecules and methods for making and using them
US6673552B2 (en) * 2002-01-14 2004-01-06 Diversa Corporation Methods for purifying annealed double-stranded oligonucleotides lacking base pair mismatches or nucleotide gaps
EP1483409B1 (de) * 2002-02-28 2009-04-15 Wisconsin Alumni Research Foundation Verfahren zur fehlerreduzierung in nukleinsäurepopulationen
US7998699B2 (en) 2002-08-15 2011-08-16 University Of South Florida Early detection of pathogens in blood
US7563600B2 (en) 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
US7879580B2 (en) * 2002-12-10 2011-02-01 Massachusetts Institute Of Technology Methods for high fidelity production of long nucleic acid molecules
US7452699B2 (en) * 2003-01-15 2008-11-18 Dana-Farber Cancer Institute, Inc. Amplification of DNA in a hairpin structure, and applications
DE602004020999D1 (de) 2003-08-15 2009-06-18 Univ South Florida Materialien und verfahren zum einfangen von krankheitserregern und zur abtrennung von aurintricarbonsäure aus einer probe
SG110153A1 (en) * 2003-09-22 2005-04-28 Agency Science Tech & Res Device and method of detecting mutations and polymorphisms in dna
WO2005063977A1 (ja) 2003-12-25 2005-07-14 Riken 核酸の増幅法およびこれを利用した変異核酸の検出法
EP1623996A1 (de) * 2004-08-06 2006-02-08 Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts Verbessertes Verfahren zur Auswahl eines Proteins von einer Bibliothek
US7820412B2 (en) * 2004-08-27 2010-10-26 Wisconsin Alumni Research Foundation Method of error reduction in nucleic acid populations
US20070122817A1 (en) * 2005-02-28 2007-05-31 George Church Methods for assembly of high fidelity synthetic polynucleotides
CA2584984A1 (en) * 2004-10-18 2006-04-27 Codon Devices, Inc. Methods for assembly of high fidelity synthetic polynucleotides
KR100681945B1 (ko) 2005-08-18 2007-02-12 학교법인 포항공과대학교 MutS 돌연변이체-형광물질 결합체의 형광발광 변화를이용한 잘못 짝지어진 DNA의 검출방법
US20070196834A1 (en) * 2005-09-09 2007-08-23 Francesco Cerrina Method and system for the generation of large double stranded DNA fragments
EP2025762A3 (de) 2006-01-17 2009-09-30 Health Research Inc. Heteroduplex-Trackingassay
US20070231805A1 (en) * 2006-03-31 2007-10-04 Baynes Brian M Nucleic acid assembly optimization using clamped mismatch binding proteins
WO2007136834A2 (en) * 2006-05-19 2007-11-29 Codon Devices, Inc. Combined extension and ligation for nucleic acid assembly
WO2008027558A2 (en) * 2006-08-31 2008-03-06 Codon Devices, Inc. Iterative nucleic acid assembly using activation of vector-encoded traits
US8603950B2 (en) 2007-02-20 2013-12-10 Anaptysbio, Inc. Methods of generating libraries and uses thereof
CA2622649C (en) 2007-03-09 2018-04-24 Riken Nucleic acid amplification method using primer exhibiting exciton effect
US9388457B2 (en) 2007-09-14 2016-07-12 Affymetrix, Inc. Locus specific amplification using array probes
US9074244B2 (en) 2008-03-11 2015-07-07 Affymetrix, Inc. Array-based translocation and rearrangement assays
WO2010061922A1 (ja) * 2008-11-27 2010-06-03 独立行政法人理化学研究所 新規MutSタンパク質およびそれを用いた変異の判定方法
ES2548400T3 (es) 2010-11-12 2015-10-16 Gen9, Inc. Métodos y dispositivos para la síntesis de ácidos nucleicos
WO2012064975A1 (en) 2010-11-12 2012-05-18 Gen9, Inc. Protein arrays and methods of using and making the same
US8672333B2 (en) * 2011-06-13 2014-03-18 John Hartman Torque transmitting apparatus for a wheel
EP2944693B1 (de) 2011-08-26 2019-04-24 Gen9, Inc. Zusammensetzungen und verfahren zur hochqualitativen anordnung von nukleinsäuren
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
EP4001427A1 (de) 2012-04-24 2022-05-25 Gen9, Inc. Verfahren zum sortieren von nukleinsäuren und zur multiplexierten, vorbereitenden in-vitro-klonung
JP6509727B2 (ja) 2012-06-25 2019-05-15 ギンゴー バイオワークス, インコーポレイテッド 核酸アセンブリおよび高処理シークエンシングのための方法
US9279146B2 (en) * 2012-12-21 2016-03-08 Roche Molecular Systems, Inc. Compounds and methods for the enrichment of mutated nucleic acid from a mixture
TWI721929B (zh) 2013-08-05 2021-03-11 美商扭轉生物科技有限公司 重新合成之基因庫
CA2975852A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
WO2016126987A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Compositions and methods for synthetic gene assembly
WO2016172377A1 (en) 2015-04-21 2016-10-27 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
IL258164B (en) 2015-09-18 2022-09-01 Twist Bioscience Corp Methods to regulate the activity of proteins and cells and a method for the production of nucleic acids
WO2017053450A1 (en) 2015-09-22 2017-03-30 Twist Bioscience Corporation Flexible substrates for nucleic acid synthesis
CN108603307A (zh) 2015-12-01 2018-09-28 特韦斯特生物科学公司 功能化表面及其制备
WO2018038772A1 (en) 2016-08-22 2018-03-01 Twist Bioscience Corporation De novo synthesized nucleic acid libraries
JP6871364B2 (ja) 2016-09-21 2021-05-12 ツイスト バイオサイエンス コーポレーション 核酸に基づくデータ保存
EA201991262A1 (ru) 2016-12-16 2020-04-07 Твист Байосайенс Корпорейшн Библиотеки вариантов иммунологического синапса и их синтез
SG11201907713WA (en) 2017-02-22 2019-09-27 Twist Bioscience Corp Nucleic acid based data storage
EP3595674A4 (de) 2017-03-15 2020-12-16 Twist Bioscience Corporation Variante bibliotheken der immunologischen synapse und synthese davon
CN111566209A (zh) 2017-06-12 2020-08-21 特韦斯特生物科学公司 无缝核酸装配方法
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
US11407837B2 (en) 2017-09-11 2022-08-09 Twist Bioscience Corporation GPCR binding proteins and synthesis thereof
KR20240024357A (ko) 2017-10-20 2024-02-23 트위스트 바이오사이언스 코포레이션 폴리뉴클레오타이드 합성을 위한 가열된 나노웰
US10936953B2 (en) 2018-01-04 2021-03-02 Twist Bioscience Corporation DNA-based digital information storage with sidewall electrodes
KR20210013128A (ko) 2018-05-18 2021-02-03 트위스트 바이오사이언스 코포레이션 핵산 하이브리드화를 위한 폴리뉴클레오타이드, 시약 및 방법
SG11202109283UA (en) 2019-02-26 2021-09-29 Twist Bioscience Corp Variant nucleic acid libraries for antibody optimization
AU2020229349A1 (en) 2019-02-26 2021-10-14 Twist Bioscience Corporation Variant nucleic acid libraries for GLP1 receptor
EP3987019A4 (de) 2019-06-21 2023-04-19 Twist Bioscience Corporation Barcode-basierte nukleinsäuresequenzanordnung
CN114457067A (zh) * 2022-02-10 2022-05-10 中国科学院天津工业生物技术研究所 一种低成本快速去除dna合成中错误的方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5459039A (en) * 1989-05-12 1995-10-17 Duke University Methods for mapping genetic mutations
US5556750A (en) * 1989-05-12 1996-09-17 Duke University Methods and kits for fractionating a population of DNA molecules based on the presence or absence of a base-pair mismatch utilizing mismatch repair systems
ATE151114T1 (de) * 1991-07-19 1997-04-15 Upstate Biotech Inc Verfahren zum nachweis von mutationen
WO1993020233A1 (en) * 1992-03-27 1993-10-14 University Of Maryland At Baltimore Detection of gene mutations with mismatch repair enzymes
WO1993022457A1 (en) * 1992-04-24 1993-11-11 Massachusetts Institute Of Technology Screening for genetic variation

Also Published As

Publication number Publication date
CA2175222A1 (en) 1995-05-11
EP0733126B1 (de) 2002-04-24
EP0733126A4 (de) 1999-03-03
WO1995012689A1 (en) 1995-05-11
US6027877A (en) 2000-02-22
AU1050895A (en) 1995-05-23
JPH09504699A (ja) 1997-05-13
AU700959B2 (en) 1999-01-14
US6114115A (en) 2000-09-05
DE69430485T2 (de) 2002-12-19
EP0733126A1 (de) 1996-09-25
DE69430485D1 (de) 2002-05-29

Similar Documents

Publication Publication Date Title
ATE216727T1 (de) Immobilisierte fehlpaarung - bildendes protein zur detektion oder reinigung von mutationen oder polymorphismen
US6238866B1 (en) Detector for nucleic acid typing and methods of using the same
Hayes et al. The missing nucleoside experiment: a new technique to study recognition of DNA by protein
US6830884B1 (en) Method of amplification
US4725537A (en) Assay, reagent and kit employing nucleic acid strand displacement and restriction endonuclease cleavage
DE60010058D1 (de) Biochemische reinigungsgeräte mit immobilisierten fangsonden und ihre anwendungen
ATE151114T1 (de) Verfahren zum nachweis von mutationen
AU2002311761B2 (en) Mutation detection using MutS and RecA
KR102592367B1 (ko) 게놈 및 치료학적 적용을 위한 핵산 분자의 클론 복제 및 증폭을 위한 시스템 및 방법
EP1961826A3 (de) Verfahren zur direkten Sequenzierung von Nukleinsäuren
WO2000077260A8 (en) Genomic profiling: a rapid method for testing a complex biological sample for the presence of many types of organisms
AU2002311761A1 (en) Mutation detection using MutS and RecA
EP0758402A1 (de) Nachweisreaktionen für mutierte nukleinsäuren
AU781962B2 (en) A method of amplification of a circularised nucleic acid probe
Wu et al. Polymerase chain reaction with sequence-specific primers-based genotyping of the human Dombrock blood group DO1 and DO2 alleles and the DO gene frequencies in Chinese blood donors.
US7049070B2 (en) Materials and methods for detection and characterization of nucleic acid sequence variability
Żaczek et al. Genotyping of clinical Mycobacterium tuberculosis isolates based on IS6110 and MIRU-VNTR polymorphisms
EP1192273B1 (de) Verfahren zum nachweis von fehlpaarungen oder mutationen in nukleinsäuren
Bellanné-Chantelot et al. Search for DNA sequence variations using a MutS-based technology
Rubin et al. Strong, specific binding of six different DNA sequences by a single conformation-switching DNA macrocycle
Holm et al. In vitro amplification of a set of VNTR loci for forensic science
JP2000308489A (ja) 微小変異dna検出方法
JP2009124960A (ja) RecA様相同組換え蛋白質を用いたDNA相同塩基配列の検出方法
ATE40409T1 (de) Photoimmunologisches testverfahren fuer dns und rns.
Sun The incision pattern and incision efficiency of (+) anti-BPDE-DNA adduct by the Escherichia coli UvrABC excision repair system

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties